##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936243_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2212016 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.63351621326428 31.0 31.0 34.0 30.0 34.0 2 31.375811929027638 31.0 31.0 34.0 30.0 34.0 3 31.73890695184845 31.0 31.0 34.0 30.0 34.0 4 35.57418888470969 37.0 35.0 37.0 33.0 37.0 5 35.21594916130805 37.0 35.0 37.0 33.0 37.0 6 35.42682964318522 37.0 35.0 37.0 33.0 37.0 7 35.2495004556929 37.0 35.0 37.0 32.0 37.0 8 35.325258045149766 37.0 35.0 37.0 32.0 37.0 9 36.98347570722816 39.0 37.0 39.0 33.0 39.0 10-11 36.86825728204498 39.0 37.0 39.0 32.0 39.0 12-13 36.819631503569596 39.0 37.0 39.0 32.0 39.0 14-15 37.92520103832884 40.0 37.0 41.0 32.5 41.0 16-17 37.83585041880348 40.0 37.0 41.0 32.0 41.0 18-19 37.99890868782143 40.0 37.0 41.0 33.0 41.0 20-21 38.06458881852572 40.0 37.0 41.0 33.0 41.0 22-23 37.9653664349625 40.0 37.0 41.0 32.5 41.0 24-25 37.939479868138385 40.0 37.0 41.0 33.0 41.0 26-27 37.762033818923555 40.0 37.0 41.0 32.0 41.0 28-29 37.6037490235152 40.0 37.0 41.0 32.0 41.0 30-31 37.39393792811626 39.5 36.0 41.0 32.0 41.0 32-33 37.144103614078745 39.0 35.5 41.0 31.0 41.0 34-35 36.920504417689564 39.0 35.0 41.0 31.0 41.0 36-37 36.697134650020615 39.0 35.0 41.0 30.0 41.0 38-39 36.66944746330949 39.0 35.0 41.0 30.5 41.0 40-41 36.672583968651224 39.0 35.0 41.0 30.0 41.0 42-43 36.544207410796304 39.0 35.0 41.0 30.0 41.0 44-45 36.32528019688827 39.5 35.0 41.0 30.0 41.0 46-47 35.95851657492532 39.0 35.0 41.0 28.5 41.0 48-49 35.628015122856254 39.0 35.0 41.0 27.0 41.0 50-51 35.44017606563425 39.0 35.0 41.0 26.0 41.0 52-53 35.31290573847567 39.0 35.0 41.0 26.0 41.0 54-55 35.14520261155435 39.0 35.0 41.0 26.0 41.0 56-57 34.95935042965331 39.0 34.5 41.0 25.5 41.0 58-59 34.753899384091255 38.0 34.0 40.0 25.0 41.0 60-61 34.54140747625695 38.0 34.0 40.0 25.0 41.0 62-63 34.32974919711249 38.0 34.0 40.0 24.0 41.0 64-65 34.0924349100549 37.0 34.0 40.0 23.5 41.0 66-67 33.81554744631142 37.0 33.5 40.0 23.0 41.0 68-69 33.510068869302934 36.5 33.0 39.0 23.0 41.0 70-71 33.18317950683901 36.0 33.0 39.0 22.0 41.0 72-73 32.91016272034199 35.5 33.0 39.0 22.5 40.5 74-75 32.70933618924998 35.0 33.0 38.0 22.0 40.0 76-77 32.400493712522874 35.0 33.0 37.0 22.0 39.5 78-79 32.05911417458101 35.0 32.5 37.0 21.5 39.0 80-81 31.769092990285785 35.0 32.0 36.0 21.0 39.0 82-83 31.501262423056616 35.0 32.0 36.0 20.0 37.5 84-85 31.229756701579014 35.0 32.0 35.5 20.0 37.0 86-87 31.02627535243868 35.0 32.0 35.0 20.0 37.0 88-89 30.85708331223644 35.0 32.0 35.0 19.5 36.0 90-91 30.711167550325136 35.0 32.0 35.0 19.0 36.0 92-93 30.573885089438775 35.0 32.0 35.0 18.0 36.0 94-95 30.44593800406507 35.0 32.0 35.0 18.0 36.0 96-97 30.37797692240924 35.0 32.0 35.0 17.0 35.0 98-99 30.291686633369743 34.0 31.0 35.0 16.0 35.0 100-101 30.21418674186805 34.0 31.0 35.0 14.0 35.0 102-103 29.204449244490092 33.5 29.5 34.5 10.0 35.0 104-105 29.94806841361003 34.0 31.0 35.0 10.0 35.0 106-107 30.089646503461097 34.0 31.0 35.0 9.0 35.0 108-109 30.058505453848436 34.0 31.0 35.0 7.0 35.0 110-111 30.02207059080947 34.0 31.0 35.0 6.0 35.0 112-113 29.942146440170415 34.0 31.0 35.0 2.0 35.0 114-115 29.91632768479071 34.0 31.0 35.0 2.0 35.0 116-117 29.81710507518933 34.0 31.0 35.0 2.0 35.0 118-119 29.691760819089914 34.0 31.0 35.0 2.0 35.0 120-121 29.496852644827165 34.0 31.0 35.0 2.0 35.0 122-123 29.282524403078458 34.0 31.0 35.0 2.0 35.0 124-125 27.247878405942814 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11625656916310234 1101 2 0.6823342155308438 1101 3 0.38664926738562144 1101 4 0.2143575263226296 1101 5 0.5446036670616579 1101 6 0.34377313295919976 1101 7 0.46726103454261647 1101 8 0.359660753129198 1101 9 0.2907806748060864 1101 10-11 0.4385835260641713 1101 12-13 0.41947150199419525 1101 14-15 0.5074640166086937 1101 16-17 0.5590427532314308 1101 18-19 0.5485689086823697 1101 20-21 0.429961814010305 1101 22-23 0.45224872445646014 1101 24-25 0.4502694614780296 1101 26-27 0.5105254204162861 1101 28-29 0.522510691877649 1101 30-31 0.4363383337036524 1101 32-33 0.4130657876583115 1101 34-35 0.4214153205147113 1101 36-37 0.43261220668868816 1101 38-39 0.3725818519542017 1101 40-41 0.4008636530117968 1101 42-43 0.5027725409553483 1101 44-45 0.5505822713367365 1101 46-47 0.4903182950800655 1101 48-49 0.5359885298844347 1101 50-51 0.4888519564433622 1101 52-53 0.4027421111408245 1101 54-55 0.4462757181405763 1101 56-57 0.1649897108489924 1101 58-59 0.34776442599627444 1101 60-61 0.4506083009409281 1101 62-63 0.491971755577552 1101 64-65 0.4884504815583881 1101 66-67 0.4796942274516667 1101 68-69 0.5011438182560113 1101 70-71 0.44186930907186195 1101 72-73 0.5149307420654097 1101 74-75 0.44174733063314164 1101 76-77 0.4660911012411546 1101 78-79 0.5834501833406165 1101 80-81 0.5081478774448946 1101 82-83 0.42092879761483815 1101 84-85 0.41142164567493467 1101 86-87 0.40941275899801255 1101 88-89 0.3837164432573239 1101 90-91 0.3463413655440384 1101 92-93 0.39718690654276756 1101 94-95 0.3775764416079923 1101 96-97 0.37140833201708645 1101 98-99 0.30486075677887925 1101 100-101 0.32278438335510273 1101 102-103 0.4131583867508475 1101 104-105 0.36841594580475956 1101 106-107 0.4836511627066109 1101 108-109 0.34677655437972277 1101 110-111 0.40377523828476214 1101 112-113 0.3896300425152681 1101 114-115 0.368879496987784 1101 116-117 0.370330576954391 1101 118-119 0.45337416264997543 1101 120-121 0.559943738894038 1101 122-123 0.5183330925832514 1101 124-125 0.4816390451647621 1102 1 0.29198278077493356 1102 2 0.665272807051764 1102 3 0.41892920039912696 1102 4 0.2617031237237555 1102 5 0.47946358723189775 1102 6 0.28096372966779626 1102 7 0.4478810373127331 1102 8 0.35939129619823973 1102 9 0.41014405132808207 1102 10-11 0.47000950725254853 1102 12-13 0.41735576142833963 1102 14-15 0.5126161842260188 1102 16-17 0.5772084314696357 1102 18-19 0.4667230027908289 1102 20-21 0.4405310732555776 1102 22-23 0.4397134369308091 1102 24-25 0.4271282736010775 1102 26-27 0.4314811111200214 1102 28-29 0.4358061249604077 1102 30-31 0.2589390967452445 1102 32-33 0.3977456501597345 1102 34-35 0.46938520177738496 1102 36-37 0.5132964258301982 1102 38-39 0.4378256852938307 1102 40-41 0.3564166063353085 1102 42-43 0.31206616086998196 1102 44-45 0.3597276899393336 1102 46-47 0.25589718678104134 1102 48-49 0.18117985689686122 1102 50-51 0.20992713996367485 1102 52-53 0.355085756867922 1102 54-55 0.4360470574926154 1102 56-57 0.29957047867533504 1102 58-59 0.3050786706984283 1102 60-61 0.3053658526602874 1102 62-63 0.25222962837877105 1102 64-65 0.24807236045387526 1102 66-67 0.20341185169775144 1102 68-69 0.11939360734930204 1102 70-71 0.2544130328659122 1102 72-73 0.25932163089554194 1102 74-75 0.2798241132670185 1102 76-77 0.29000124868697696 1102 78-79 0.35600599492222784 1102 80-81 0.28299898360337394 1102 82-83 0.17692131829629076 1102 84-85 0.27716687036927823 1102 86-87 0.15223412416251847 1102 88-89 0.20071653139744328 1102 90-91 0.16999439567536712 1102 92-93 0.19047528841612404 1102 94-95 0.10522927027619389 1102 96-97 0.09914917488843855 1102 98-99 0.21710190814064134 1102 100-101 0.27685821959076407 1102 102-103 0.28803857914813236 1102 104-105 0.2067916493517714 1102 106-107 0.11151540174260433 1102 108-109 0.14377148002723672 1102 110-111 0.19860922007753246 1102 112-113 0.14829748985155078 1102 114-115 0.12460419762616937 1102 116-117 0.22292680707564472 1102 118-119 0.16897383023581725 1102 120-121 0.30648310604799534 1102 122-123 0.3242185859790361 1102 124-125 0.32399226039033735 1103 1 0.14316375005705595 1103 2 0.561360186102764 1103 3 0.3341140569504475 1103 4 0.20589038869185572 1103 5 0.39806670549783263 1103 6 0.2533041192397718 1103 7 0.34405376727428205 1103 8 0.35058578641668703 1103 9 0.25088601270890365 1103 10-11 0.35390438197262597 1103 12-13 0.38619803125551755 1103 14-15 0.3432584287014464 1103 16-17 0.45728828949980027 1103 18-19 0.3823908102864948 1103 20-21 0.4049541095508431 1103 22-23 0.36627447128243773 1103 24-25 0.24412240044436118 1103 26-27 0.20997479430758403 1103 28-29 0.25088703931373146 1103 30-31 0.1396785229505113 1103 32-33 0.17869718605359708 1103 34-35 0.16533272364326734 1103 36-37 0.07846184603555173 1103 38-39 0.09129887581227081 1103 40-41 0.16142019783401906 1103 42-43 0.022592632328134243 1103 44-45 -0.07257720717478833 1103 46-47 0.01642957364883557 1103 48-49 -0.07009195617619213 1103 50-51 -0.02666642914098105 1103 52-53 -0.03691111641555267 1103 54-55 -9.200274780170048E-4 1103 56-57 -0.10284469857569434 1103 58-59 -0.0729950184045478 1103 60-61 -0.08267530168965465 1103 62-63 -0.19379479381246512 1103 64-65 -0.1482845922651066 1103 66-67 -0.1397427517585328 1103 68-69 -0.21628856614120906 1103 70-71 -0.12077942921208518 1103 72-73 -8.693523300493666E-4 1103 74-75 -0.039507022914158085 1103 76-77 -0.02294969566591476 1103 78-79 -0.011904175357059898 1103 80-81 4.642305717261763E-4 1103 82-83 -0.028779023952413496 1103 84-85 0.005433655344138089 1103 86-87 -0.06508915297442286 1103 88-89 -5.442959148496129E-4 1103 90-91 0.002256617334218447 1103 92-93 -0.0030139582142041377 1103 94-95 -0.018511803793185067 1103 96-97 -0.033865148853479354 1103 98-99 0.033221821944675156 1103 100-101 0.05235279008977045 1103 102-103 0.12003333978530506 1103 104-105 0.02887387354579829 1103 106-107 0.011933705710159614 1103 108-109 -0.007834976219857737 1103 110-111 -0.0020482354512623147 1103 112-113 0.02677891969056745 1103 114-115 0.02755345186202618 1103 116-117 0.065666909327728 1103 118-119 0.010063533197747887 1103 120-121 0.04214964595588455 1103 122-123 -0.008876440851569356 1103 124-125 0.10299255616081737 1104 1 0.18019098699697977 1104 2 0.438340922167054 1104 3 0.2521763874160037 1104 4 0.1275404047409765 1104 5 0.34692954620905425 1104 6 0.144467123435831 1104 7 0.28078380441179007 1104 8 0.24112778106476185 1104 9 0.32894445147027795 1104 10-11 0.1571383216246005 1104 12-13 0.19503369656230518 1104 14-15 0.23934518709481267 1104 16-17 0.35604843246171924 1104 18-19 0.28606880336458573 1104 20-21 0.2764070457086021 1104 22-23 0.24199747175310193 1104 24-25 0.15333717428479332 1104 26-27 0.06041626989545534 1104 28-29 0.18693644399940723 1104 30-31 0.1059951579901437 1104 32-33 0.13566391863530214 1104 34-35 0.18850100326055497 1104 36-37 0.23912395131446118 1104 38-39 0.1770280464634979 1104 40-41 0.22394242129433906 1104 42-43 0.222078644645201 1104 44-45 0.17591600479502745 1104 46-47 0.20693739115118603 1104 48-49 0.18338022562294043 1104 50-51 0.09941505715739396 1104 52-53 0.07520497278763116 1104 54-55 0.0963813085074463 1104 56-57 0.19725883073432726 1104 58-59 0.13737179966160795 1104 60-61 0.08338845573410936 1104 62-63 0.026849865322475353 1104 64-65 -0.001981212303391544 1104 66-67 -0.015197158115654474 1104 68-69 0.01787951556462275 1104 70-71 0.011785652887908782 1104 72-73 -0.05617346240208576 1104 74-75 -0.011460475471125164 1104 76-77 0.05722340201420195 1104 78-79 0.04505598789493348 1104 80-81 -0.05788414015134791 1104 82-83 -0.07343632946283307 1104 84-85 -0.10032149326407946 1104 86-87 -0.057870623805765575 1104 88-89 -0.019161111070541637 1104 90-91 -0.04821620809291716 1104 92-93 -0.0628131529308007 1104 94-95 -0.07277721136604143 1104 96-97 -0.10876024746826474 1104 98-99 -0.14354146099053366 1104 100-101 -0.15381772309961406 1104 102-103 -0.15955916219796862 1104 104-105 -0.09987625742501649 1104 106-107 -0.11955059691807435 1104 108-109 -0.17019645899241453 1104 110-111 -0.16248199415562325 1104 112-113 -0.14287069388316453 1104 114-115 -0.13019461517507125 1104 116-117 -0.11129337034277498 1104 118-119 -0.08345786036497316 1104 120-121 -0.19178884183431322 1104 122-123 -0.18381203647357225 1104 124-125 -0.21814089436713857 1105 1 0.14440669691250818 1105 2 0.48627306695597383 1105 3 0.22088912564572993 1105 4 0.17540329328031135 1105 5 0.4255218305269892 1105 6 0.23954564281028468 1105 7 0.3161707438685113 1105 8 0.3009054238483344 1105 9 0.34114418746838027 1105 10-11 0.2318107671691294 1105 12-13 0.2509506078992061 1105 14-15 0.2401712282240851 1105 16-17 0.36634938618441737 1105 18-19 0.37958727091296396 1105 20-21 0.2613654895231079 1105 22-23 0.21838446008703727 1105 24-25 0.2791171596021442 1105 26-27 0.24068691812258436 1105 28-29 0.26435957240406793 1105 30-31 0.18329174962460826 1105 32-33 0.24685157968855975 1105 34-35 0.2194734454437608 1105 36-37 0.07772717373549654 1105 38-39 0.020084129086448854 1105 40-41 0.06990612972049348 1105 42-43 0.09478513016415491 1105 44-45 0.2096958826149944 1105 46-47 0.06505682984592909 1105 48-49 -0.031247895921865165 1105 50-51 0.020395462591430658 1105 52-53 -0.014767582901136223 1105 54-55 0.09466102548726951 1105 56-57 0.08626127930405403 1105 58-59 0.13621222433151559 1105 60-61 0.019275802040851886 1105 62-63 0.08810234957581997 1105 64-65 0.016372586941827194 1105 66-67 0.1529034295638212 1105 68-69 0.1580651255611656 1105 70-71 0.0548733108396533 1105 72-73 0.002840981382547625 1105 74-75 0.055525297687623265 1105 76-77 0.0569535624532449 1105 78-79 0.035527894945744265 1105 80-81 0.05177830017907681 1105 82-83 0.005049512952165713 1105 84-85 -0.021376675961739977 1105 86-87 0.0955288431565684 1105 88-89 0.09165664204237345 1105 90-91 0.05028128041404045 1105 92-93 0.0014002998642865805 1105 94-95 0.10016534621772877 1105 96-97 0.038753006619028696 1105 98-99 0.07581451596994881 1105 100-101 0.07168996255787974 1105 102-103 0.08502480422517422 1105 104-105 0.06243144687401525 1105 106-107 -0.00898547079294687 1105 108-109 -0.035987427880037615 1105 110-111 0.052922763561984 1105 112-113 0.023118836650393604 1105 114-115 0.04465395071717637 1105 116-117 0.047774391822983375 1105 118-119 0.06741428123150328 1105 120-121 0.04194876775173029 1105 122-123 0.09430831481231294 1105 124-125 0.22776149020753778 1106 1 0.13197422416762805 1106 2 0.5159353270548905 1106 3 0.24542457036955057 1106 4 0.12305638407909925 1106 5 0.3229487592073639 1106 6 0.14455569468443485 1106 7 0.20444235075195394 1106 8 0.23723420840662612 1106 9 0.2295397409371489 1106 10-11 0.10989827135758645 1106 12-13 0.0784696424668141 1106 14-15 0.06572416614195475 1106 16-17 0.0710106504282706 1106 18-19 0.05266449698838471 1106 20-21 0.0536749271021435 1106 22-23 -0.06939268556854472 1106 24-25 0.008704211434036324 1106 26-27 -5.311436510169187E-6 1106 28-29 -0.0717600139116783 1106 30-31 -0.04995027208155989 1106 32-33 -0.10549668694591219 1106 34-35 -0.09312970029471757 1106 36-37 -0.035855852983551983 1106 38-39 -0.10105669015199936 1106 40-41 -0.09308451877627988 1106 42-43 -0.07059383328849123 1106 44-45 -0.02535855490303618 1106 46-47 -0.018083857042775264 1106 48-49 -0.02732923655527486 1106 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52-53 -0.6799754536059197 2216 54-55 -0.8287795714424107 2216 56-57 -1.336169528854441 2216 58-59 -1.4155534169117843 2216 60-61 -0.9670506267962438 2216 62-63 -0.9762919287130813 2216 64-65 -0.7711150441310224 2216 66-67 -0.6244346318546476 2216 68-69 -0.6738098993757831 2216 70-71 -0.636519914876601 2216 72-73 -0.6839395903351289 2216 74-75 -0.6786014184718212 2216 76-77 -0.5010799363841976 2216 78-79 -0.5239698849654069 2216 80-81 -0.5695774829449682 2216 82-83 -0.5582518872790558 2216 84-85 -0.44423077223106233 2216 86-87 -0.3032797417991624 2216 88-89 -0.5141820441506404 2216 90-91 -0.42034887117251074 2216 92-93 -0.4586267648963549 2216 94-95 -0.4562910001338487 2216 96-97 -0.5785674406833046 2216 98-99 -0.5532226583799797 2216 100-101 -0.723579376341501 2216 102-103 -0.7443098013290559 2216 104-105 -0.4722738307543075 2216 106-107 -0.4102094552122999 2216 108-109 -0.4126795950347173 2216 110-111 -0.5244635912187867 2216 112-113 -0.4285386381877707 2216 114-115 -0.4808217933463723 2216 116-117 -0.34437583620733747 2216 118-119 -0.4038380039270315 2216 120-121 -0.5909308290056039 2216 122-123 -0.4874950176815922 2216 124-125 -0.6904448473475426 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 94.0 3 100.0 4 248.0 5 496.0 6 1311.0 7 2488.0 8 3982.0 9 7250.0 10 12190.0 11 15532.0 12 16778.0 13 21154.0 14 28821.0 15 21742.0 16 10202.0 17 8505.0 18 8034.0 19 7774.0 20 7706.0 21 7770.0 22 8717.0 23 10297.0 24 12876.0 25 16739.0 26 21276.0 27 27171.0 28 34167.0 29 43355.0 30 54890.0 31 68941.0 32 86537.0 33 109816.0 34 148158.0 35 207764.0 36 343724.0 37 583100.0 38 251121.0 39 1190.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.48465267435948 18.18404305176816 10.829712813293355 21.501591460579007 2 18.813172068531212 21.46604858739592 37.75649702036244 21.964282323710428 3 17.522493076445265 28.74299168088448 32.244642212933556 21.4898730297367 4 10.798023160772798 16.759688899176137 37.84809874792949 34.594189192121576 5 9.856083279350615 42.60032642957698 35.588423692562685 11.955166598509715 6 29.289706765231355 38.1344438738237 18.84308250934894 13.73276685159601 7 26.2579927089135 34.9544035847842 22.730622201647726 16.056981504654576 8 27.8940116165525 33.12891950148643 20.397049569261704 18.580019312699367 9 27.85798113576032 11.18106740638404 19.959756168128983 41.00119528972665 10-11 23.97007978242472 26.14108577876471 30.199984991066977 19.688849447743596 12-13 25.367899689694827 25.649249372518103 30.00102621319195 18.98182472459512 14-15 22.56303751871596 26.64512824500365 28.316386499916817 22.47544773636357 16-17 20.705772471808523 28.696243607641176 28.973524603800332 21.62445931674997 18-19 19.917532242081432 26.674309769911247 32.656296337820336 20.751861650186978 20-21 21.31783856897961 25.99176497819184 32.93615416886677 19.75424228396178 22-23 20.87588878199796 26.305212078031985 32.59137818171297 20.227520958257085 24-25 20.370557898315383 26.417937302442656 32.930729253314624 20.280775545927334 26-27 20.28805397429313 26.395062241864437 32.941104404308106 20.375779379534325 28-29 20.294880326362918 26.2249911393046 32.95014593022835 20.52998260410413 30-31 20.56341364619424 26.059847668371294 33.07455732689095 20.30218135854352 32-33 20.437035717643994 26.330550954423476 32.80611442231883 20.426298905613702 34-35 20.717545187327026 26.4228210128869 32.703228822240725 20.15640497754535 36-37 20.94548592776906 26.22788442759908 32.15519236750548 20.671437277126387 38-39 20.703918958994873 26.619970199130567 32.19287292677811 20.483237915096456 40-41 20.72560070216506 26.455329992036674 32.197446175218936 20.621623130579326 42-43 20.685658097729956 26.513085099770002 31.836638279356 20.96461852314404 44-45 20.840516413139554 26.798686135017036 31.53795898806173 20.82283846378168 46-47 21.133126621436006 26.947215221808374 30.59819855012311 21.321459606632505 48-49 21.200566865153867 27.294796781012504 30.08816068805078 21.41647566578285 50-51 21.235471398525394 27.292792800938948 30.024133709248936 21.447602091286726 52-53 21.303820728944782 27.388337055299626 29.926146151997735 21.38169606375786 54-55 21.161894105081124 27.659585530294038 29.90552079977847 21.27299956484637 56-57 21.24779261973201 27.707629375314774 29.69931253611152 21.345265468841692 58-59 21.395928776532994 27.171041522609457 29.758160202844607 21.674869498012946 60-61 21.612998382661587 27.353815099001228 29.880276269881907 21.152910248455278 62-63 21.230418901742844 27.341712980617196 30.204411283703585 21.223456833936375 64-65 21.014794471004418 27.23109438397812 30.361645136133998 21.392466008883464 66-67 21.17415323075574 27.679720825705267 29.74904739095771 21.397078552581284 68-69 21.463557646078073 27.445220792918246 29.795295510588073 21.29592605041561 70-71 21.213106216302478 27.112788760003582 30.090354337650172 21.583750686043768 72-73 21.481533220131176 27.38097498521681 29.539524008441337 21.597967786210674 74-75 21.122573093969095 27.656664324139463 29.540842817027226 21.679919764864216 76-77 21.031712215515128 27.63333224080895 29.700938403098547 21.63401714057737 78-79 21.264381975968956 27.46341737968597 29.4396937450879 21.83250689925717 80-81 21.15820609970945 27.529004202970427 29.224432834073312 22.088356863246815 82-83 21.33115372035478 27.471916423656545 29.319735164364957 21.877194691623718 84-85 21.0457647531112 27.389412696518278 29.469762315506998 22.095060234863524 86-87 20.790403834343312 27.535789306415413 29.42063739809318 22.253169461148094 88-89 21.267039513271076 27.235599059851513 29.382691882561907 22.114669544315504 90-91 21.29514076393427 27.365507021187092 29.40620940436491 21.933142810513733 92-93 21.18903303899286 27.464961126940118 29.173002351935967 22.17300348213105 94-95 21.013283760999446 27.269379808601908 29.366250652976404 22.351085777422238 96-97 21.31187116732908 27.32377766490784 29.14144328703782 22.222907880725256 98-99 20.986867921525796 27.29465530554415 29.097987873351933 22.620488899578117 100-101 20.918780215895314 27.37111425959698 29.120663761902136 22.58944176260557 102-103 21.151102813946018 27.106345590789875 29.002389466005994 22.740162129258106 104-105 20.924876475105034 27.035603695925438 29.20081274735777 22.838707081611762 106-107 20.904283261437808 27.1236606048989 29.094948205384615 22.877107928278676 108-109 20.999174275366165 27.282163421144656 28.583295678813236 23.135366624675942 110-111 20.94434793812394 27.221827280936616 28.66963305873298 23.16419172220646 112-113 20.886693471635276 27.101600114658837 28.902028638485127 23.10967777522076 114-115 20.746462445709348 27.387901427546634 28.650702446631758 23.214933680112264 116-117 20.65996627800614 27.391207232006686 28.56860580942507 23.380220680562104 118-119 20.739376983154546 27.16963550195908 28.572164897181153 23.518822617705222 120-121 20.559587825368304 27.33667950944853 28.58267951487379 23.521053150309378 122-123 20.603253169032232 27.254310146057144 28.38145391645275 23.76098276845788 124-125 20.4074242188602 27.15093747626471 28.385150115556968 24.056488189318124 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 118.0 1 124.5 2 608.5 3 1161.5 4 940.0 5 902.0 6 1445.5 7 3128.0 8 4830.0 9 5517.5 10 4845.5 11 3507.0 12 3250.5 13 3387.0 14 3629.0 15 3954.0 16 4322.0 17 4697.5 18 5086.0 19 5497.0 20 6025.0 21 6703.0 22 7799.5 23 9268.5 24 10768.0 25 13268.5 26 17198.5 27 21220.5 28 24239.5 29 27627.5 30 33351.0 31 39112.0 32 44448.0 33 50972.5 34 58396.0 35 63595.5 36 69834.0 37 76499.5 38 76256.0 39 75264.0 40 73759.0 41 72353.5 42 72827.0 43 73666.5 44 76950.0 45 79102.5 46 78316.0 47 76299.5 48 75197.0 49 73206.0 50 71123.5 51 68279.0 52 63898.5 53 61075.0 54 57277.5 55 52311.0 56 47521.0 57 43464.0 58 40078.5 59 35218.0 60 29214.5 61 23857.5 62 19369.0 63 14554.0 64 10213.5 65 7635.5 66 6040.5 67 4597.0 68 3242.5 69 2192.0 70 1517.0 71 1207.5 72 1082.0 73 931.0 74 654.0 75 420.5 76 264.0 77 184.0 78 112.0 79 39.5 80 14.5 81 4.0 82 2.0 83 1.5 84 2.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06636480025460938 2 9.041525920246508E-5 3 9.041525920246508E-5 4 0.0 5 0.064737325588965 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 9.041525920246508E-5 36-37 0.0 38-39 0.0 40-41 2.260381480061627E-5 42-43 0.002418608183665941 44-45 0.009990886141872391 46-47 0.010940246363498276 48-49 0.00915454499424959 50-51 0.007775712291411997 52-53 0.0066681253661818 54-55 0.006939371143789195 56-57 0.00533450029294544 58-59 0.0034583836644942892 60-61 0.0021925700356597785 62-63 0.00133362507323636 64-65 0.0018535128136505342 66-67 0.002915892109279499 68-69 0.0010171716660277322 70-71 0.0026220425168714872 72-73 0.001989135702454232 74-75 7.911335180215695E-4 76-77 6.103029996166393E-4 78-79 1.1301907400308134E-4 80-81 4.068686664110929E-4 82-83 1.1301907400308134E-4 84-85 3.6166103680986033E-4 86-87 3.390572220092441E-4 88-89 0.0011301907400308134 90-91 0.0014692479620400576 92-93 3.842648516104766E-4 94-95 0.00133362507323636 96-97 0.0013788327028375924 98-99 0.0012658136288345111 100-101 8.137373328221858E-4 102-103 7.007182588191044E-4 104-105 5.650953700154067E-4 106-107 4.5207629601232537E-4 108-109 8.815487772240346E-4 110-111 6.103029996166393E-4 112-113 0.010714208215492111 114-115 0.019190638765723213 116-117 0.018218674729296714 118-119 0.011844398955522925 120-121 0.006238652884970091 122-123 0.006419483403375021 124-125 0.005424915552147905 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2212016.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.170392863202 #Duplication Level Percentage of deduplicated Percentage of total 1 40.785776696816335 10.265940226896964 2 15.986957453667028 8.047959995921893 3 12.023355163559136 9.078977190017754 4 8.94994342189067 9.010943681296746 5 6.298100134675617 7.926282734078536 6 4.2711254135513075 6.450354277625552 7 2.813099065816681 4.956476605479868 8 1.9089347417222724 3.8438909919491717 9 1.3352551896992537 3.0248007927623584 >10 5.172924189860443 21.560165066528466 >50 0.27901278526313655 4.890216740243371 >100 0.16605988381762354 7.620946463044059 >500 0.007091894745243625 1.1915489460450956 >1k 0.0018184345500624676 0.727067760455051 >5k 3.636869100124936E-4 0.8381075078182209 >10k+ 1.818434550062468E-4 0.5663210198369749 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12373 0.5593540010560503 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9214 0.41654309914575666 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9097 0.4112538064824124 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2506 0.11329031978068874 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.3562288880369762E-4 0.0 14-15 0.0 0.0 0.0 1.8083051840493016E-4 0.0 16-17 0.0 0.0 0.0 1.8083051840493016E-4 0.0 18-19 0.0 0.0 0.0 1.8083051840493016E-4 0.0 20-21 0.0 0.0 0.0 2.2603814800616268E-4 0.0 22-23 0.0 0.0 0.0 2.7124577760739523E-4 0.0 24-25 0.0 0.0 0.0 4.068686664110929E-4 0.0 26-27 0.0 0.0 0.0 5.424915552147906E-4 0.0 28-29 0.0 0.0 0.0 8.36341147622802E-4 0.0 30-31 0.0 0.0 0.0 0.0024638158132671735 0.0 32-33 0.0 0.0 0.0 0.008159977143022475 0.0 34-35 0.0 0.0 0.0 0.01618433139724125 0.0 36-37 0.0 0.0 0.0 0.028277372315570953 0.0 38-39 0.0 0.0 0.0 0.04936673152454593 0.0 40-41 0.0 0.0 0.0 0.07400488965721766 0.0 42-43 0.0 0.0 0.0 0.10259871537999725 0.0 44-45 0.0 0.0 0.0 0.14163550354066154 0.0 46-47 0.0 0.0 0.0 0.1893521565847625 0.0 48-49 0.0 0.0 0.0 0.23779213170248314 0.0 50-51 0.0 0.0 0.0 0.28645814496821 0.0 52-53 0.0 0.0 0.0 0.3403456394528792 0.0 54-55 0.0 0.0 0.0 0.4064165901150805 0.0 56-57 0.0 0.0 0.0 0.4946392792818859 0.0 58-59 0.0 0.0 0.0 0.5970571641434781 0.0 60-61 0.0 0.0 0.0 0.6988195383758526 0.0 62-63 0.0 0.0 0.0 0.7990448532017851 4.520762960123254E-5 64-65 0.0 0.0 0.0 0.9157031413877657 4.520762960123254E-5 66-67 0.0 0.0 0.0 1.0629217871841794 4.520762960123254E-5 68-69 0.0 0.0 0.0 1.2491320135116564 4.520762960123254E-5 70-71 0.0 0.0 0.0 1.4330140469146697 4.520762960123254E-5 72-73 0.0 0.0 0.0 1.6082162154342465 4.520762960123254E-5 74-75 0.0 0.0 0.0 1.8009137366095 4.520762960123254E-5 76-77 0.0 0.0 0.0 2.0331679336858324 4.520762960123254E-5 78-79 0.0 0.0 0.0 2.318880152765622 4.520762960123254E-5 80-81 0.0 0.0 0.0 2.610220721730765 4.520762960123254E-5 82-83 0.0 0.0 0.0 2.895865129366153 4.520762960123254E-5 84-85 0.0 0.0 0.0 3.1950266182523093 4.520762960123254E-5 86-87 0.0 0.0 0.0 3.54717144903111 4.520762960123254E-5 88-89 0.0 0.0 0.0 3.951915356850945 4.520762960123254E-5 90-91 0.0 0.0 0.0 4.375985526325307 4.520762960123254E-5 92-93 0.0 0.0 0.0 4.797591879986402 4.520762960123254E-5 94-95 0.0 0.0 0.0 5.227358210790519 4.520762960123254E-5 96-97 0.0 0.0 0.0 5.720347411591959 4.520762960123254E-5 98-99 0.0 0.0 0.0 6.260555981511888 4.520762960123254E-5 100-101 0.0 0.0 0.0 6.848549015920319 4.520762960123254E-5 102-103 0.0 0.0 0.0 7.423228403411187 4.520762960123254E-5 104-105 0.0 0.0 0.0 8.0289202248085 4.520762960123254E-5 106-107 0.0 0.0 0.0 8.68070122458427 4.520762960123254E-5 108-109 0.0 0.0 0.0 9.361437711119631 4.520762960123254E-5 110-111 0.0 0.0 0.0 10.09976871776696 4.520762960123254E-5 112-113 0.0 0.0 0.0 10.803493283954547 4.520762960123254E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7090 0.0 85.455025 1 GTATCAA 11285 0.0 53.4903 2 GTACATG 18685 0.0 48.814064 1 TACATGG 19230 0.0 46.96182 2 ACATGGG 19120 0.0 46.76391 3 TACCTGG 1950 0.0 44.57146 2 CATGGGA 14395 0.0 42.717678 4 CATGGGG 4555 0.0 41.42761 4 TCAACGC 16035 0.0 38.90553 5 ATCAACG 16120 0.0 38.70038 4 CAACGCA 16295 0.0 38.482185 6 ATGGGAT 6365 0.0 37.59643 5 AACGCAG 16730 0.0 37.588287 7 TATCAAC 16945 0.0 37.027794 3 ATGGGAG 5000 0.0 35.597557 5 ACGCAGA 19380 0.0 32.387108 8 GTCGGGA 590 0.0 32.287663 2 CGCAGAG 19625 0.0 31.800892 9 GAGTACA 11610 0.0 31.762985 1 AGTACAT 11425 0.0 31.57585 2 >>END_MODULE