FastQCFastQC Report
Thu 26 May 2016
SRR936242_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936242_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887381
Sequences flagged as poor quality0
Sequence length125
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71830.8094606488081219No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35780.4032089936566142No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20090.22639655345336446No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG17020.19180036534476172No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16190.18244699852712645No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT11460.1291440767832532No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11450.12903138561677566No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10210.11505768097356153No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9620.10840890215138707No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9300.10480278482410599No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG91000.054.1328961
TACATGG93550.050.2406922
GAACAAA17650.046.8535461
ACATGGG96150.045.788333
CATGGGG24050.043.2907684
ATGGGAT23400.042.9678085
CATGGGA69100.042.0159454
GTCGGGA5600.041.4332432
GGTATCA23700.041.1687661
ATGGGAG20950.039.757255
CGCAGAA2050.037.7278332
TCGGGAG6050.037.3680573
CGGGAGT6300.035.88524
GAGAACA16000.034.952661
ATGGGTA4300.033.205895
GTACAAG9100.032.688951
TACCTGG4050.032.317672
GTATCAA30300.032.201312
GTATAGG4250.032.1966931
ATGGGGG14300.032.0351685