##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936242_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 887381 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.827590403671028 31.0 28.0 31.0 12.0 34.0 2 28.09317869100195 31.0 28.0 34.0 12.0 34.0 3 28.440978565013225 31.0 28.0 34.0 16.0 34.0 4 31.359072371394024 35.0 32.0 37.0 12.0 37.0 5 32.24508300267867 35.0 32.0 37.0 22.0 37.0 6 31.653519739548177 35.0 32.0 37.0 13.0 37.0 7 31.73943097722399 35.0 32.0 37.0 13.0 37.0 8 31.32576649714159 35.0 32.0 37.0 10.0 37.0 9 32.408830029040516 38.0 32.0 39.0 8.0 39.0 10-11 32.572337023217756 38.0 32.0 39.0 9.5 39.0 12-13 32.56078223446299 38.0 32.0 39.0 9.0 39.0 14-15 33.56179927224045 39.0 32.0 41.0 9.0 41.0 16-17 33.65018971557876 39.0 32.0 41.0 11.0 41.0 18-19 33.70694774848684 39.0 32.0 41.0 12.5 41.0 20-21 33.83040937320047 39.0 32.0 41.0 14.5 41.0 22-23 33.72809819006717 39.0 32.0 41.0 15.5 41.0 24-25 33.71563736433392 39.0 32.0 41.0 16.5 41.0 26-27 33.77273572456476 39.0 32.0 41.0 17.0 41.0 28-29 33.622409652674556 39.0 32.0 41.0 16.0 41.0 30-31 33.51150464118569 38.0 31.5 41.0 15.0 41.0 32-33 33.32022209175089 38.0 31.0 40.0 11.5 41.0 34-35 33.2482603301175 38.0 31.0 40.0 9.0 41.0 36-37 33.09278089118428 38.0 31.0 40.0 8.0 41.0 38-39 32.81233258318581 38.0 30.0 40.0 7.5 41.0 40-41 32.876263408840174 38.0 30.5 40.0 2.0 41.0 42-43 32.75149118586042 38.0 30.0 40.0 2.0 41.0 44-45 32.656462669360735 38.0 30.0 40.0 2.0 41.0 46-47 32.61138845659305 38.0 30.0 40.0 2.0 41.0 48-49 32.482024068579335 38.0 30.0 40.0 2.0 41.0 50-51 32.32937205101304 38.0 30.0 40.0 2.0 41.0 52-53 32.167256229285954 38.0 30.0 40.0 2.0 41.0 54-55 31.959839122090735 37.0 30.0 40.0 2.0 41.0 56-57 31.748108197042757 37.0 30.0 40.0 2.0 41.0 58-59 31.484201261915683 37.0 30.0 40.0 2.0 41.0 60-61 31.22283889332767 36.0 30.0 40.0 2.0 41.0 62-63 30.9460564289747 36.0 29.5 40.0 2.0 41.0 64-65 30.652999106359047 35.5 29.0 39.0 2.0 41.0 66-67 30.369575751565563 35.0 29.0 39.0 2.0 41.0 68-69 30.093025994471372 35.0 29.0 39.0 2.0 40.5 70-71 29.760157136562537 35.0 29.0 38.0 2.0 40.0 72-73 29.45745626737557 35.0 28.5 37.5 2.0 40.0 74-75 29.129059558408393 34.5 28.0 37.0 2.0 39.0 76-77 28.81354119594627 34.0 28.0 36.5 2.0 39.0 78-79 28.493766488126298 34.0 28.0 36.0 2.0 39.0 80-81 28.19840012350952 34.0 27.5 35.5 2.0 37.5 82-83 27.91117062456825 34.0 27.0 35.0 2.0 37.0 84-85 27.89364264053434 34.0 27.5 35.0 2.0 37.0 86-87 27.76972687041981 34.0 27.0 35.0 2.0 36.0 88-89 27.580308796334382 34.0 27.0 35.0 2.0 36.0 90-91 27.40001532599864 34.0 27.0 35.0 2.0 36.0 92-93 27.249663898595983 34.0 27.0 35.0 2.0 35.5 94-95 27.113341394508108 34.0 26.0 35.0 2.0 35.0 96-97 27.004133511986396 34.0 26.0 35.0 2.0 35.0 98-99 26.87404451977223 34.0 25.5 35.0 2.0 35.0 100-101 26.74504581459373 34.0 25.0 35.0 2.0 35.0 102-103 25.839651739219118 32.5 24.0 34.5 2.0 35.0 104-105 26.420218598324734 33.5 25.0 35.0 2.0 35.0 106-107 26.5077869596036 34.0 25.0 35.0 2.0 35.0 108-109 26.43551247998323 34.0 25.0 35.0 2.0 35.0 110-111 26.29827886781439 34.0 24.5 35.0 2.0 35.0 112-113 26.224675759341253 34.0 24.0 35.0 2.0 35.0 114-115 26.083957736304924 34.0 24.0 35.0 2.0 35.0 116-117 25.93654304070067 34.0 23.5 35.0 2.0 35.0 118-119 25.727618689153815 34.0 21.5 35.0 2.0 35.0 120-121 25.51366267702374 34.0 19.5 35.0 2.0 35.0 122-123 25.192047158999348 33.5 9.5 35.0 2.0 35.0 124-125 23.349845782138676 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6476654610175068 1101 2 0.6774381667198952 1101 3 0.5106337017148199 1101 4 0.6888943578195246 1101 5 0.6527028990812127 1101 6 0.6878290199825088 1101 7 0.6184923390510022 1101 8 0.6199704843993779 1101 9 0.5429112825935007 1101 10-11 0.6898913526241515 1101 12-13 0.6152027034163297 1101 14-15 0.7283190605358882 1101 16-17 0.763779016928332 1101 18-19 0.811844892171365 1101 20-21 0.5953077651823477 1101 22-23 0.791345931950751 1101 24-25 0.922661281395861 1101 26-27 0.704336449807009 1101 28-29 0.8066932649373655 1101 30-31 0.9059741424661851 1101 32-33 0.9893781535266157 1101 34-35 0.9374298855435796 1101 36-37 0.7775546596115035 1101 38-39 0.8330802617250441 1101 40-41 0.8453421575908848 1101 42-43 0.9164672963750107 1101 44-45 0.9584367445059101 1101 46-47 0.7384066434088368 1101 48-49 0.8064144901665031 1101 50-51 0.8881143078453917 1101 52-53 0.73083382382422 1101 54-55 0.6523929126092902 1101 56-57 0.7782557639480459 1101 58-59 0.8354890205583736 1101 60-61 0.6840874200035323 1101 62-63 0.5941603663055446 1101 64-65 0.776291835037032 1101 66-67 0.7956292940365657 1101 68-69 0.8816218547828036 1101 70-71 0.8808231264736257 1101 72-73 0.8310546605001932 1101 74-75 0.9037284123089258 1101 76-77 0.8846697859836326 1101 78-79 0.7567999125106262 1101 80-81 0.6552194570663659 1101 82-83 0.590360624973453 1101 84-85 0.43922762477849275 1101 86-87 0.6735650324459748 1101 88-89 0.5375832179713562 1101 90-91 0.5323317114550079 1101 92-93 0.33311758633270117 1101 94-95 0.5108979636326652 1101 96-97 0.5961854993936093 1101 98-99 0.5996366753756739 1101 100-101 0.6571798104494242 1101 102-103 0.6796013879130314 1101 104-105 0.7261360150447587 1101 106-107 0.6217542413063697 1101 108-109 0.6861633615306886 1101 110-111 0.6789077534811909 1101 112-113 0.6236793659719808 1101 114-115 0.8319735978014116 1101 116-117 0.8025581346918251 1101 118-119 0.7172149961544321 1101 120-121 0.8272301978782473 1101 122-123 0.5800010276273326 1101 124-125 0.3207887041865547 1102 1 0.4377879264860347 1102 2 0.3040359408457931 1102 3 0.29577076778033984 1102 4 0.4607425116108175 1102 5 0.45193434161022594 1102 6 0.44880964146172175 1102 7 0.21625798637737859 1102 8 0.3281276172461105 1102 9 0.5316347839438365 1102 10-11 0.6515073681449195 1102 12-13 0.5017998291809533 1102 14-15 0.6988253558744191 1102 16-17 0.4404385100594439 1102 18-19 0.4345918991113251 1102 20-21 0.48223854762970575 1102 22-23 0.6034847510853965 1102 24-25 0.7222503201005424 1102 26-27 0.5167722682758296 1102 28-29 0.5805393689095766 1102 30-31 0.5928725068502061 1102 32-33 0.8220443397409554 1102 34-35 0.6268024962699599 1102 36-37 0.61028568195254 1102 38-39 0.7174624856540675 1102 40-41 0.6379603151975317 1102 42-43 0.7429194385855737 1102 44-45 0.715926439302315 1102 46-47 0.7845652275873221 1102 48-49 0.7209911476646766 1102 50-51 0.7478403502731581 1102 52-53 0.7254287351857513 1102 54-55 0.8282187627730089 1102 56-57 0.7691517742819229 1102 58-59 0.759321783250666 1102 60-61 0.7765921143884746 1102 62-63 0.652916859269574 1102 64-65 0.6892947202446678 1102 66-67 0.6216976562510688 1102 68-69 0.5819901738563438 1102 70-71 0.6111252877609701 1102 72-73 0.694406538628229 1102 74-75 0.5967605236475144 1102 76-77 0.6327270711952622 1102 78-79 0.5794703629471485 1102 80-81 0.6183142350150987 1102 82-83 0.6723853075881436 1102 84-85 0.6266855748097875 1102 86-87 0.7163142338394515 1102 88-89 0.6954237759610393 1102 90-91 0.7634101606746064 1102 92-93 0.7025555817991567 1102 94-95 0.7114981965439782 1102 96-97 0.8412962320169015 1102 98-99 0.8477286229781136 1102 100-101 0.9419261141667796 1102 102-103 0.8641693922819904 1102 104-105 0.8313792627362204 1102 106-107 0.5980027731421558 1102 108-109 0.6044309402255372 1102 110-111 0.501567481019837 1102 112-113 0.579735932998819 1102 114-115 0.6492451938424146 1102 116-117 0.6771929950821871 1102 118-119 0.798502541882602 1102 120-121 0.8550281884570587 1102 122-123 0.849729289445591 1102 124-125 1.0331345001871632 1103 1 0.2874497134623084 1103 2 0.2130844012193407 1103 3 0.4311594603598472 1103 4 0.43632555048007404 1103 5 0.24717159552238854 1103 6 0.36118218058337703 1103 7 0.27001114134962023 1103 8 0.3559418524808393 1103 9 0.5217483629443436 1103 10-11 0.4712032288213521 1103 12-13 0.4409505106549574 1103 14-15 0.7447579656674463 1103 16-17 0.5375447695243665 1103 18-19 0.478337482150188 1103 20-21 0.3271363265292493 1103 22-23 0.456702822766367 1103 24-25 0.5384163978390575 1103 26-27 0.3599234545453882 1103 28-29 0.2641461331700512 1103 30-31 0.326452769343895 1103 32-33 0.39662283545019505 1103 34-35 0.34737922519474296 1103 36-37 0.30428492475919455 1103 38-39 0.41301397480096114 1103 40-41 0.37835279374372277 1103 42-43 0.5676481109732592 1103 44-45 0.4994776760514341 1103 46-47 0.3612083371279624 1103 48-49 0.3637864530659911 1103 50-51 0.3151535759167743 1103 52-53 0.353862606967688 1103 54-55 0.23524677892647006 1103 56-57 0.24861416701286743 1103 58-59 0.15916655861664353 1103 60-61 0.1259447140855947 1103 62-63 0.15644159425190907 1103 64-65 0.24496483634764843 1103 66-67 0.3001201701329457 1103 68-69 0.1663932531092449 1103 70-71 0.10311544424759234 1103 72-73 0.2692437420608371 1103 74-75 0.1874382116838582 1103 76-77 0.13021192732852782 1103 78-79 0.17245622867152832 1103 80-81 0.1813783440509411 1103 82-83 0.16539767507931202 1103 84-85 0.23558512049378422 1103 86-87 0.1724336179888688 1103 88-89 0.17717415455770436 1103 90-91 0.24361460287551395 1103 92-93 0.31609293667043303 1103 94-95 0.3308760360189922 1103 96-97 0.31284216841266144 1103 98-99 0.25744088951016764 1103 100-101 0.29024135899262404 1103 102-103 0.17452401116134197 1103 104-105 0.020823987622641482 1103 106-107 0.061632253202731135 1103 108-109 0.05649429206803802 1103 110-111 0.1625076627462363 1103 112-113 0.3158010516257761 1103 114-115 0.3619622559322693 1103 116-117 0.3280372656526076 1103 118-119 0.3934192506155689 1103 120-121 0.3530090062258644 1103 122-123 0.3886843626409444 1103 124-125 0.3282808908989274 1104 1 0.21739159171253775 1104 2 0.15572996051372456 1104 3 0.13929293644835283 1104 4 -0.04392427107595509 1104 5 0.019084005025803208 1104 6 0.024232042926602304 1104 7 -0.027683872416059074 1104 8 0.12485181475268803 1104 9 0.022818516915997122 1104 10-11 -0.1163232828539833 1104 12-13 -0.104083903538978 1104 14-15 0.0389052584175289 1104 16-17 -0.013319154203585981 1104 18-19 0.03346953068695058 1104 20-21 0.011479397804869507 1104 22-23 0.14174666899864263 1104 24-25 0.38650704737510466 1104 26-27 0.134938494822741 1104 28-29 0.27096014357078957 1104 30-31 0.2619266655404928 1104 32-33 0.3261242120179588 1104 34-35 0.3326461263877718 1104 36-37 0.4267446474062879 1104 38-39 0.50417717455624 1104 40-41 0.3042554653048555 1104 42-43 0.4099992377194539 1104 44-45 0.6163834576360117 1104 46-47 0.4571897789552253 1104 48-49 0.1550995457548865 1104 50-51 0.2504260853488134 1104 52-53 0.42734659799450014 1104 54-55 0.48097865411765284 1104 56-57 0.2985575747828264 1104 58-59 0.13440898749397334 1104 60-61 0.14922171010884355 1104 62-63 0.01252118026189919 1104 64-65 0.12439509024792272 1104 66-67 0.07942219929579153 1104 68-69 0.12942297677601644 1104 70-71 0.16884629975896104 1104 72-73 0.07562515329770747 1104 74-75 0.10367380870639664 1104 76-77 0.010657272490146141 1104 78-79 -0.05502771137129514 1104 80-81 -0.11656292239139887 1104 82-83 -0.08342994804702641 1104 84-85 0.03203561503849883 1104 86-87 0.012552155768009499 1104 88-89 0.009928054833018507 1104 90-91 -0.020981603915554814 1104 92-93 0.002309669616291643 1104 94-95 0.003256733479986451 1104 96-97 -0.0889297801954747 1104 98-99 -0.11028420481021683 1104 100-101 -0.13487717878651395 1104 102-103 -7.627737881854557E-4 1104 104-105 0.01903648889724252 1104 106-107 -0.032326143860590406 1104 108-109 -0.12983307613834327 1104 110-111 0.042225466355905183 1104 112-113 0.04627775194187578 1104 114-115 -0.0991367039943114 1104 116-117 0.10867278110075418 1104 118-119 0.03604084743416536 1104 120-121 0.08574072846507974 1104 122-123 0.24475757565205924 1104 124-125 0.2117625876461453 1105 1 0.6668169897846035 1105 2 0.717644460537187 1105 3 0.6742563461729958 1105 4 0.725356692348587 1105 5 0.5601251298773064 1105 6 0.5846967628868853 1105 7 0.5439335338360252 1105 8 0.44862598495735995 1105 9 0.685739959582996 1105 10-11 0.8822005100867969 1105 12-13 0.6875286124839306 1105 14-15 0.7565337936457013 1105 16-17 0.8339350315115084 1105 18-19 0.6903098004892882 1105 20-21 0.5035438895544644 1105 22-23 0.4744116710115449 1105 24-25 0.5414440794999535 1105 26-27 0.5836765440164697 1105 28-29 0.6008861232837432 1105 30-31 0.7887031400804219 1105 32-33 0.916632721757658 1105 34-35 0.9284531253430401 1105 36-37 0.8153390522925648 1105 38-39 0.9212381468227164 1105 40-41 0.8970613948312192 1105 42-43 0.9593621495298592 1105 44-45 0.8356887487157891 1105 46-47 0.8893398250172311 1105 48-49 0.885124811938951 1105 50-51 0.8084098784377858 1105 52-53 0.7319236549162795 1105 54-55 0.7405520186694226 1105 56-57 0.864657172450336 1105 58-59 0.8381692773511951 1105 60-61 0.9854590492314195 1105 62-63 0.9668481686463721 1105 64-65 0.9142938032285173 1105 66-67 0.9977647573796098 1105 68-69 0.850620885338607 1105 70-71 0.8339125277868895 1105 72-73 0.8230956946065611 1105 74-75 0.6944092942345286 1105 76-77 0.6068048586186912 1105 78-79 0.7747226646576877 1105 80-81 0.9338301483020501 1105 82-83 0.7911156681588949 1105 84-85 0.6561535831729763 1105 86-87 0.6421728666295046 1105 88-89 0.7453192361197409 1105 90-91 0.7031524180015616 1105 92-93 0.6507184927752299 1105 94-95 0.5822539401218094 1105 96-97 0.7538715801773641 1105 98-99 0.6285629854073456 1105 100-101 0.6649138750578736 1105 102-103 0.7904718608894719 1105 104-105 0.6725936366587248 1105 106-107 0.6854335384226999 1105 108-109 0.6623704660670526 1105 110-111 0.8606453295776788 1105 112-113 0.7430699591888015 1105 114-115 0.7425962153984109 1105 116-117 0.7243459656031739 1105 118-119 0.6785947325730568 1105 120-121 0.6156881955486533 1105 122-123 0.6323768984788103 1105 124-125 0.7757574109186969 1106 1 -0.19333279055053154 1106 2 -0.4326218588127624 1106 3 -0.21541357334801958 1106 4 -0.18651152817161076 1106 5 0.012468545763162808 1106 6 -0.23334831700891456 1106 7 -0.38005707694893687 1106 8 -0.35204370129122964 1106 9 -0.4894996306351125 1106 10-11 -0.26439468450128345 1106 12-13 -0.2052834379806754 1106 14-15 -0.43192140832933745 1106 16-17 -0.31024214861367483 1106 18-19 -0.3504827425689143 1106 20-21 -0.2877854230694652 1106 22-23 -0.4099304035578726 1106 24-25 -4.3231004560935276 1106 26-27 -1.3773618584562115 1106 28-29 -1.0036809133789077 1106 30-31 -0.9945624794522487 1106 32-33 -5.361414885256064 1106 34-35 -1.401487147196903 1106 36-37 -0.9544329725120662 1106 38-39 -0.4712757523258446 1106 40-41 -1.0169762592257783 1106 42-43 -0.8451227558004177 1106 44-45 -0.6340616497912634 1106 46-47 -0.7010950336585537 1106 48-49 -0.42721675720688523 1106 50-51 -0.4375831817076232 1106 52-53 -0.3544760768204398 1106 54-55 -0.4139887748456097 1106 56-57 -0.38390188435149497 1106 58-59 -0.22527766289218576 1106 60-61 -0.18819453150027954 1106 62-63 -0.2106089675458449 1106 64-65 -0.08020169655764775 1106 66-67 -0.31452068058936433 1106 68-69 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-0.9741318600306954 2216 82-83 -1.0350323540752093 2216 84-85 -1.102359485920207 2216 86-87 -1.0325007843434904 2216 88-89 -1.0795824052090595 2216 90-91 -1.0445923942090332 2216 92-93 -1.061392486070087 2216 94-95 -1.1823528269547694 2216 96-97 -1.2905033126369005 2216 98-99 -1.140066399119572 2216 100-101 -0.9786726351764727 2216 102-103 -0.8526990209086271 2216 104-105 -1.0443217849721123 2216 106-107 -1.0704377176427506 2216 108-109 -1.2545479819844374 2216 110-111 -1.2493509378368515 2216 112-113 -1.218557548957314 2216 114-115 -1.152257860685804 2216 116-117 -1.1448271658342755 2216 118-119 -1.3374553849529462 2216 120-121 -1.2239589238032913 2216 122-123 -1.1146246752599254 2216 124-125 -0.9721710041448084 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44090.0 3 8462.0 4 8271.0 5 8077.0 6 8807.0 7 6477.0 8 5517.0 9 5545.0 10 6506.0 11 7028.0 12 6726.0 13 6057.0 14 5896.0 15 5588.0 16 5205.0 17 4830.0 18 4296.0 19 3957.0 20 3736.0 21 3931.0 22 4729.0 23 7879.0 24 10959.0 25 13611.0 26 16998.0 27 13692.0 28 14969.0 29 18221.0 30 21011.0 31 25356.0 32 32612.0 33 41634.0 34 56599.0 35 81497.0 36 129410.0 37 178218.0 38 60833.0 39 151.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.50658172757812 20.592507615105575 9.09282484073921 20.808085816577098 2 17.833489786236125 25.241919761635646 35.568712875303845 21.355877576824387 3 16.07032379552864 35.31944001505554 27.142907048945155 21.467329140470664 4 9.843573391812537 19.79273840661452 34.60520340192093 35.75848479965201 5 10.24095706677425 47.22530871849426 31.341547795866028 11.192186418865466 6 26.885888796231605 41.268678581892765 18.45962270954946 13.385809912326174 7 27.257063200586895 38.63154608899672 19.964141670826848 14.147249039589536 8 26.050003493437973 38.1970253939133 18.765509174218877 16.987461938429846 9 27.13328637836315 19.65392308775884 17.301366389632342 35.91142414424567 10-11 22.302690519791522 32.97647555993802 26.160078884349907 18.56075503592055 12-13 23.310577050939063 31.71808053875818 25.717059548735932 19.254282861566825 14-15 21.76062278172607 32.41459205171216 23.11585820401443 22.708926962547338 16-17 21.332654174348527 34.151911486952486 24.07164075494832 20.443793583750672 18-19 20.693294356819912 32.82039550492916 25.708327510897345 20.777982627353577 20-21 22.51695034154241 31.540792103420046 25.521892465556984 20.420365089480562 22-23 22.27969631720888 31.830635146399157 25.220255745936925 20.669412790455038 24-25 21.787049083615862 31.97824816720355 25.527525895240277 20.70717685394031 26-27 21.388231674711943 31.83563629069383 25.745810529613518 21.03032150498071 28-29 21.3964099752585 30.939044406792842 26.31721294987297 21.34733266807569 30-31 21.34772329266671 31.580960988440097 26.057779210212555 21.01353650868063 32-33 20.928356106493872 31.935878292717284 25.99740808564587 21.138357515142978 34-35 21.25993657719709 31.504161698847614 26.226534802530598 21.00936692142469 36-37 21.621764770182754 31.39656223552732 25.36497933802806 21.61669365626187 38-39 21.323764323791934 31.69543774161758 26.013364083082923 20.967433851507558 40-41 21.150975768486354 31.58695143836755 26.592008474409145 20.67006431873695 42-43 21.133677606204802 31.606165358035334 25.81049215243794 21.449664883321923 44-45 20.99522413221874 31.83333370897182 25.965372141297173 21.206070017512264 46-47 21.30779815118039 31.751499080999395 25.770726534494358 21.16997623332586 48-49 21.00554441163876 31.896200049584166 25.64493227253262 21.45332326624445 50-51 21.22443023025189 31.732320910189145 25.671305061214188 21.371943798344784 52-53 21.2095668631825 31.724788435110053 25.77724987899711 21.28839482271034 54-55 21.13516641470672 32.0616446344928 25.711634238679977 21.0915547121205 56-57 21.23427879823277 32.17529425221425 25.248076774627233 21.342350174925752 58-59 21.041243840019114 31.485179421240705 25.786499823638326 21.687076915101855 60-61 21.191891406100773 32.298188092017 25.400688206117 21.109232295765228 62-63 20.804223681578584 32.28006119154475 25.622860620198278 21.292854506678385 64-65 20.491582514348437 32.01354776256819 25.928887206030343 21.56598251705303 66-67 20.668463344559285 32.49915340522674 25.21725509464113 21.615128155572847 68-69 20.943822326599285 32.11405247576858 25.381882190400738 21.560243007231392 70-71 20.69985761463093 31.68432256512285 25.82009633973251 21.795723480513715 72-73 20.814678249816033 32.35825423352539 25.02329889866923 21.80376861798934 74-75 20.53785239936397 32.473480951248675 25.337876289891266 21.650790359496092 76-77 20.31675233073505 32.20296580611935 26.070763291077903 21.40951857206769 78-79 20.498804910179505 31.960229033526748 25.779851044816148 21.761115011477592 80-81 20.350514801711327 32.666482979961806 25.185982788668447 21.797019429658416 82-83 20.673983328468832 32.02733662316412 25.374388227829986 21.924291820537064 84-85 20.280071356046612 31.720478576845796 25.7537630397766 22.245687027330987 86-87 20.45485535525327 32.072807508837805 25.838901216050374 21.633435919858552 88-89 20.790968474064957 31.541711813590673 25.92518091168332 21.742138800661046 90-91 20.511651703157945 31.749778280129952 25.88532997663912 21.85324004007298 92-93 20.44465680468705 31.850355146211157 25.55041182986789 22.154576219233903 94-95 20.164799654714074 31.385240040771688 26.22843301154353 22.221527292970716 96-97 20.80470508158277 31.623620519258356 25.76542657550703 21.806247823651848 98-99 20.37126146633911 31.836642700984925 25.649270887331244 22.14282494534472 100-101 19.99768982848385 31.90369402059997 25.915278685568754 22.183337465347428 102-103 20.325114381662875 31.976999707002633 25.625267641821992 22.072618269512496 104-105 19.64740001318489 32.68336715013137 25.655596055577124 22.01363678110662 106-107 19.595596252115076 32.728801230137464 25.92692762574792 21.748674891999542 108-109 20.12727347513271 32.98156765896929 24.84514816361189 22.04601070228611 110-111 19.818634530096762 33.682545066682295 24.848106137227646 21.650714265993297 112-113 19.479964074056127 33.282040070724975 25.597629428621975 21.64036642659692 114-115 19.498727153274636 33.660400662173295 25.03935738989228 21.80151479465979 116-117 19.441389258265907 33.518954675561766 25.19523766593793 21.844418400234396 118-119 19.3316497235116 33.15336513485219 25.23200346188044 22.282981679755775 120-121 19.34041785931619 33.12616917216975 25.303872072843653 22.2295408956704 122-123 19.500552187338005 33.26230025468232 25.06699497396831 22.170152584011362 124-125 19.671493795502606 33.15595733735416 24.559081476322728 22.61346739082051 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5636.0 1 6798.5 2 7604.5 3 6001.5 4 3749.0 5 2342.0 6 1802.0 7 1599.0 8 1476.5 9 1380.0 10 1200.5 11 1066.0 12 1051.0 13 1025.0 14 1036.0 15 1009.0 16 1017.0 17 1065.5 18 1126.0 19 1327.5 20 1578.0 21 1872.5 22 2200.0 23 2721.0 24 3283.0 25 3944.5 26 4809.0 27 5835.0 28 7052.0 29 8154.0 30 10227.5 31 12789.0 32 15878.0 33 19326.5 34 22748.0 35 25693.5 36 29097.5 37 31817.5 38 31959.5 39 32019.5 40 30683.5 41 29937.5 42 29217.0 43 28536.5 44 29444.5 45 30306.0 46 29138.5 47 26753.5 48 25961.0 49 24947.0 50 24779.0 51 23473.0 52 21728.5 53 22031.5 54 21933.0 55 20487.5 56 19019.5 57 17758.5 58 16793.0 59 16365.5 60 15286.5 61 13637.5 62 11518.5 63 8653.0 64 6333.5 65 5237.0 66 4102.5 67 3333.5 68 2578.5 69 1621.5 70 1245.5 71 1023.0 72 911.0 73 913.0 74 702.0 75 482.5 76 313.5 77 209.0 78 161.5 79 105.5 80 59.5 81 39.0 82 28.0 83 22.0 84 12.5 85 9.5 86 10.0 87 6.5 88 4.5 89 5.0 90 4.0 91 1.5 92 2.0 93 3.5 94 3.5 95 2.5 96 1.5 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 3.3807349943259997E-4 6 1.1269116647753332E-4 7 0.0 8 3.3807349943259997E-4 9 6.761469988651999E-4 10-11 6.761469988651999E-4 12-13 7.888381653427334E-4 14-15 8.451837485814998E-4 16-17 0.0010142204982978 18-19 0.0010142204982978 20-21 0.0010705660815365666 22-23 0.0899275508490716 24-25 8.451837485814998E-4 26-27 8.451837485814998E-4 28-29 6.198014156264332E-4 30-31 3.3807349943259997E-4 32-33 6.761469988651999E-4 34-35 3.3807349943259997E-4 36-37 2.2538233295506664E-4 38-39 2.817279161938333E-4 40-41 3.944190826713667E-4 42-43 3.944190826713667E-4 44-45 3.3807349943259997E-4 46-47 4.507646659101333E-4 48-49 1.1269116647753332E-4 50-51 5.634558323876666E-4 52-53 6.198014156264332E-4 54-55 5.071102491489E-4 56-57 5.071102491489E-4 58-59 0.0 60-61 1.6903674971629999E-4 62-63 3.944190826713667E-4 64-65 2.2538233295506664E-4 66-67 2.817279161938333E-4 68-69 0.0 70-71 5.634558323876666E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.634558323876666E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 5.634558323876666E-5 90-91 0.0 92-93 0.0 94-95 5.634558323876666E-5 96-97 0.0 98-99 1.1269116647753332E-4 100-101 1.1269116647753332E-4 102-103 1.1269116647753332E-4 104-105 1.6903674971629999E-4 106-107 5.634558323876666E-5 108-109 5.634558323876666E-5 110-111 1.6903674971629999E-4 112-113 0.0 114-115 0.0 116-117 1.1269116647753332E-4 118-119 2.2538233295506664E-4 120-121 1.1269116647753332E-4 122-123 1.1269116647753332E-4 124-125 5.634558323876666E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 887381.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.657466202628555 #Duplication Level Percentage of deduplicated Percentage of total 1 65.69109649457458 20.79613667090606 2 9.903290703761602 6.270261814982769 3 6.425044240720046 6.1020186270296435 4 4.440347309160309 5.622805794707006 5 3.1313648815678734 4.956553895316645 6 2.1625438955615617 4.10763961712645 7 1.4847403945188515 3.290218320141055 8 1.1144515998336182 2.8224571084958474 9 0.8312351560106154 2.3683318972058456 >10 4.327700964509804 24.518363925953736 >50 0.31952598579978564 7.03413276566008 >100 0.1601187094760711 8.726370923906108 >500 0.005693109670251445 1.1990889063353485 >1k 0.002490735480735007 1.3765022485127252 >5k 3.558193543907153E-4 0.8091174837206959 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7183 0.8094606488081219 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3578 0.4032089936566142 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2009 0.22639655345336446 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1702 0.19180036534476172 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1619 0.18244699852712645 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1146 0.1291440767832532 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1145 0.12903138561677566 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1021 0.11505768097356153 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 962 0.10840890215138707 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 930 0.10480278482410599 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1269116647753332E-4 0.0 12-13 0.0 0.0 0.0 1.1269116647753332E-4 0.0 14-15 0.0 0.0 0.0 2.2538233295506664E-4 0.0 16-17 0.0 0.0 0.0 2.2538233295506664E-4 0.0 18-19 0.0 0.0 0.0 4.5076466591013333E-4 0.0 20-21 0.0 0.0 0.0 6.198014156264333E-4 0.0 22-23 0.0 0.0 0.0 0.0011832572480141 0.0 24-25 0.0 0.0 0.0 0.0020284409965955997 0.0 26-27 0.0 0.0 0.0 0.0028736247451770997 0.0 28-29 0.0 0.0 0.0 0.0043949554926238 0.0 30-31 0.0 0.0 0.0 0.009578749150590333 0.0 32-33 0.0 0.0 0.0 0.022256505379312834 0.0 34-35 0.0 0.0 0.0 0.0351596439409904 0.0 36-37 0.0 0.0 0.0 0.05431714224217106 0.0 38-39 0.0 0.0 0.0 0.08536355860673149 0.0 40-41 0.0 0.0 0.0 0.12277702587727256 0.0 42-43 0.0 0.0 0.0 0.16554332355549645 0.0 44-45 0.0 0.0 0.0 0.2187335541328922 0.0 46-47 0.0 0.0 0.0 0.2820659896932659 0.0 48-49 0.0 0.0 0.0 0.35035683657865113 0.0 50-51 0.0 0.0 0.0 0.42011266862824426 0.0 52-53 0.0 0.0 0.0 0.493587309171596 0.0 54-55 0.0 0.0 0.0 0.5735416917874059 0.0 56-57 0.0 0.0 0.0 0.6881486080950572 0.0 58-59 0.0 0.0 0.0 0.8214622580379791 0.0 60-61 0.0 0.0 0.0 0.9470565630771901 0.0 62-63 0.0 0.0 0.0 1.0652695967121226 0.0 64-65 0.0 0.0 0.0 1.1957096219098673 0.0 66-67 0.0 0.0 0.0 1.3730855179455048 0.0 68-69 0.0 0.0 0.0 1.58573374908861 0.0 70-71 0.0 0.0 0.0 1.7986073625646708 0.0 72-73 0.0 0.0 0.0 2.004944888385034 0.0 74-75 0.0 0.0 0.0 2.229482037591519 0.0 76-77 0.0 0.0 0.0 2.479712772754882 0.0 78-79 0.0 0.0 0.0 2.8017841265476724 0.0 80-81 0.0 0.0 0.0 3.123799134757224 0.0 82-83 0.0 0.0 0.0 3.4453070327176265 0.0 84-85 0.0 0.0 0.0 3.778985576657602 0.0 86-87 0.0 0.0 0.0 4.145231867709586 0.0 88-89 0.0 0.0 0.0 4.5620764925099815 0.0 90-91 0.0 0.0 0.0 5.0211803047394525 0.0 92-93 0.0 0.0 0.0 5.473409955813794 0.0 94-95 0.0 0.0 0.0 5.942374245110049 0.0 96-97 0.0 0.0 0.0 6.452808883670036 0.0 98-99 0.0 0.0 0.0 7.015982988141509 0.0 100-101 0.0 0.0 0.0 7.637418425681866 0.0 102-103 0.0 0.0 0.0 8.252993922565391 0.0 104-105 0.0 0.0 0.0 8.88074006542849 0.0 106-107 0.0 0.0 0.0 9.511866943285916 0.0 108-109 0.0 0.0 0.0 10.16316553994282 0.0 110-111 0.0 0.0 0.0 10.910364319272105 0.0 112-113 0.0 0.0 0.0 11.635475629971793 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9100 0.0 54.132896 1 TACATGG 9355 0.0 50.240692 2 GAACAAA 1765 0.0 46.853546 1 ACATGGG 9615 0.0 45.78833 3 CATGGGG 2405 0.0 43.290768 4 ATGGGAT 2340 0.0 42.967808 5 CATGGGA 6910 0.0 42.015945 4 GTCGGGA 560 0.0 41.433243 2 GGTATCA 2370 0.0 41.168766 1 ATGGGAG 2095 0.0 39.75725 5 CGCAGAA 205 0.0 37.727833 2 TCGGGAG 605 0.0 37.368057 3 CGGGAGT 630 0.0 35.8852 4 GAGAACA 1600 0.0 34.95266 1 ATGGGTA 430 0.0 33.20589 5 GTACAAG 910 0.0 32.68895 1 TACCTGG 405 0.0 32.31767 2 GTATCAA 3030 0.0 32.20131 2 GTATAGG 425 0.0 32.196693 1 ATGGGGG 1430 0.0 32.035168 5 >>END_MODULE