FastQCFastQC Report
Thu 26 May 2016
SRR936242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936242_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887381
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133101.4999194258159685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120371.3564635708900687No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119241.3437294690781074No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38010.42833912378110417No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31780.3581325270656009No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29410.3314247206104255No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19550.22031123046357765No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16980.1913496006788516No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12430.14007511993157393No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG10670.12024147463152805No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT10540.11877648946732013No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10150.11438153397469633No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10140.11426884280821879No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51300.076.9823461
TATAACG950.062.661672
CACGCAG2300.054.352195
ATCACGC1251.0913936E-1147.622873
TATTCGC255.328176E-447.6094544-45
GTATCAA83350.047.351472
TATCACG1301.8189894E-1145.791222
GTACATG81950.043.2477041
ACATGGG83300.042.234573
TCACGCA1455.820766E-1141.0541954
TACATGG86100.040.9993632
GGGTATG3400.040.2443777
ATGGGAT21600.039.9613155
CATGGGG20150.038.996394
CATGGGA60800.038.869824
CGGGAGT5100.038.5184944
ATGGGTA4700.037.996975
GTCGGGA5500.035.717152
ATGGGAG19350.035.686395
TCGGGAG5750.035.199513
Produced by FastQC (version 0.11.5)