##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936242_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 887381 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56888867352355 31.0 31.0 34.0 30.0 34.0 2 31.28813891665474 31.0 31.0 34.0 28.0 34.0 3 31.63384611570453 31.0 31.0 34.0 30.0 34.0 4 35.50417464426216 37.0 35.0 37.0 33.0 37.0 5 35.1674883730889 37.0 35.0 37.0 33.0 37.0 6 35.39793617397713 37.0 35.0 37.0 33.0 37.0 7 35.20986363241945 37.0 35.0 37.0 32.0 37.0 8 35.30264001595707 37.0 35.0 37.0 32.0 37.0 9 36.918460052671854 39.0 37.0 39.0 33.0 39.0 10-11 36.809602639677884 39.0 37.0 39.0 32.0 39.0 12-13 36.756602293716 39.0 37.0 39.0 32.0 39.0 14-15 37.73560398521041 40.0 37.0 41.0 32.0 41.0 16-17 37.678666773347636 39.5 36.5 41.0 32.0 41.0 18-19 37.92105025913334 40.0 37.0 41.0 32.0 41.0 20-21 38.00999063536406 40.0 37.0 41.0 33.0 41.0 22-23 37.853523458356676 40.0 37.0 41.0 32.0 41.0 24-25 37.78934583904771 40.0 37.0 41.0 32.0 41.0 26-27 37.54012594364765 40.0 36.0 41.0 32.0 41.0 28-29 37.33612281534087 39.5 36.0 41.0 32.0 41.0 30-31 36.963427208831376 39.0 35.5 41.0 31.0 41.0 32-33 36.52376149590762 39.0 35.0 41.0 30.5 41.0 34-35 36.18637203185554 38.0 35.0 40.5 30.0 41.0 36-37 35.86619107238041 38.0 35.0 40.0 30.0 41.0 38-39 35.75644001843627 38.5 35.0 40.5 29.5 41.0 40-41 35.659725642086094 39.0 35.0 41.0 29.0 41.0 42-43 35.42889300086434 39.0 35.0 41.0 27.0 41.0 44-45 35.05901072932596 39.0 35.0 41.0 25.0 41.0 46-47 34.46396474569548 38.5 34.0 41.0 21.5 41.0 48-49 33.955990155299695 38.0 33.5 41.0 16.5 41.0 50-51 33.743816917423295 38.0 33.0 40.5 14.5 41.0 52-53 33.63756267037496 38.0 33.0 40.0 13.5 41.0 54-55 33.46183488264905 38.0 33.0 40.0 11.0 41.0 56-57 33.272727272727266 38.0 33.0 40.0 8.5 41.0 58-59 33.05933358951792 37.0 33.0 40.0 8.0 41.0 60-61 32.84583792080291 37.0 33.0 40.0 7.0 41.0 62-63 32.61834037465306 36.5 33.0 40.0 6.0 41.0 64-65 32.376405963165766 36.0 32.0 40.0 2.0 41.0 66-67 32.092637773402856 36.0 32.0 39.0 2.0 41.0 68-69 31.800243074846094 35.0 32.0 39.0 2.0 41.0 70-71 31.476916904914575 35.0 31.0 39.0 2.0 40.5 72-73 31.203217670876434 35.0 31.0 37.5 2.0 40.0 74-75 30.99219782708893 35.0 31.0 37.0 2.0 39.5 76-77 30.686783918069015 35.0 31.0 37.0 2.0 39.0 78-79 30.352661370933117 35.0 31.0 36.0 2.0 39.0 80-81 30.074438150016736 35.0 31.0 36.0 2.0 38.0 82-83 29.813564861091233 35.0 31.0 35.5 2.0 37.0 84-85 29.541827580261465 35.0 30.5 35.0 2.0 37.0 86-87 29.334447661151184 35.0 30.0 35.0 2.0 36.0 88-89 29.15154820759065 34.0 30.0 35.0 2.0 36.0 90-91 28.98903345913424 34.0 30.0 35.0 2.0 36.0 92-93 28.82585157897228 34.0 30.0 35.0 2.0 36.0 94-95 28.676934146663044 34.0 30.0 35.0 2.0 35.0 96-97 28.585185506563697 34.0 30.0 35.0 2.0 35.0 98-99 28.48160260361671 34.0 29.0 35.0 2.0 35.0 100-101 28.352976906199252 34.0 29.0 35.0 2.0 35.0 102-103 27.337883051361253 33.0 26.5 34.5 2.0 35.0 104-105 28.021999569519743 34.0 29.0 35.0 2.0 35.0 106-107 28.129314803900467 34.0 29.0 35.0 2.0 35.0 108-109 28.05481974484466 34.0 29.0 35.0 2.0 35.0 110-111 27.97604073109521 34.0 29.0 35.0 2.0 35.0 112-113 27.862632285343047 34.0 29.0 35.0 2.0 35.0 114-115 27.79999008317735 34.0 29.0 35.0 2.0 35.0 116-117 27.667264117667607 34.0 29.0 35.0 2.0 35.0 118-119 27.50159458000566 34.0 29.0 35.0 2.0 35.0 120-121 27.278243505326348 34.0 28.0 35.0 2.0 35.0 122-123 27.024057310219625 34.0 27.0 35.0 2.0 35.0 124-125 25.084158890037088 31.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16231826151729933 1101 2 0.5607501052352148 1101 3 0.4803883315945967 1101 4 0.2832134200213119 1101 5 0.5059450758665491 1101 6 0.24163970349373898 1101 7 0.3534489043818496 1101 8 0.40694849425733537 1101 9 0.28005524082966105 1101 10-11 0.4813815695279118 1101 12-13 0.41528653624064304 1101 14-15 0.49465726883619965 1101 16-17 0.5675918485791982 1101 18-19 0.5299471939021316 1101 20-21 0.3514065302825671 1101 22-23 0.3164446903572795 1101 24-25 0.2563586923360859 1101 26-27 0.36157193760453055 1101 28-29 0.3073615011436672 1101 30-31 0.0989679762945741 1101 32-33 0.11674757270819214 1101 34-35 -0.04458927447791439 1101 36-37 0.23222827172935467 1101 38-39 0.16574105285921092 1101 40-41 0.09737673530699453 1101 42-43 0.11760532425289227 1101 44-45 0.040690300118043865 1101 46-47 0.07996567354305739 1101 48-49 0.3424967097576257 1101 50-51 0.16452845295040675 1101 52-53 0.25443625298053973 1101 54-55 0.30134314509756877 1101 56-57 0.14703216269257524 1101 58-59 0.2220950292197159 1101 60-61 0.4512838256900338 1101 62-63 0.2922846452727015 1101 64-65 0.17826395859495392 1101 66-67 0.16688627848002113 1101 68-69 0.2646790506475192 1101 70-71 0.20918589839860147 1101 72-73 0.0686004753474947 1101 74-75 0.2342624959569548 1101 76-77 0.1728411488819681 1101 78-79 0.23158382489071982 1101 80-81 0.22645059540825585 1101 82-83 0.029293763066476686 1101 84-85 0.0010745741516586804 1101 86-87 -0.0713611613390519 1101 88-89 -0.008011181624208774 1101 90-91 0.03304340775144965 1101 92-93 0.016227522406996542 1101 94-95 0.04503387704319195 1101 96-97 0.039861939856706385 1101 98-99 -0.023217277877392917 1101 100-101 -0.044078883033787264 1101 102-103 -0.26564944623893894 1101 104-105 -0.24330722246839187 1101 106-107 -0.12341876941713181 1101 108-109 -0.28351403851808143 1101 110-111 -0.19357994915042553 1101 112-113 -0.1667817824159954 1101 114-115 -0.4102418028970476 1101 116-117 -0.28296667277951215 1101 118-119 -0.15072848290048668 1101 120-121 -0.18147212644269217 1101 122-123 -0.03184722050953326 1101 124-125 -0.0798708910698096 1102 1 0.16406833819848643 1102 2 0.5859663711231811 1102 3 0.36888715082898216 1102 4 0.1875108083722523 1102 5 0.4447128403418361 1102 6 0.28991618468752023 1102 7 0.5408283029519652 1102 8 0.47863730079880185 1102 9 0.40401443396900305 1102 10-11 0.3182142249912303 1102 12-13 0.3657230579320725 1102 14-15 0.29464754341719157 1102 16-17 0.3926924069179947 1102 18-19 0.44508318767785937 1102 20-21 0.44237460846495935 1102 22-23 0.35626703937015236 1102 24-25 0.5221578848769397 1102 26-27 0.5498039312406249 1102 28-29 0.5022845830541414 1102 30-31 0.46762594328301077 1102 32-33 0.43570590552199917 1102 34-35 0.3764891996786588 1102 36-37 0.4938979017395013 1102 38-39 0.22150261550961403 1102 40-41 0.12428248103466188 1102 42-43 0.2343183321254969 1102 44-45 0.261374520750401 1102 46-47 0.38575005352266345 1102 48-49 0.3362836632327131 1102 50-51 0.24892937960827766 1102 52-53 0.3854583446438582 1102 54-55 0.3530187849179214 1102 56-57 0.41169896734381695 1102 58-59 0.3409678282202435 1102 60-61 0.3673636838485379 1102 62-63 0.29438284208027454 1102 64-65 0.3590013947251336 1102 66-67 0.29066866848926765 1102 68-69 0.2507397821486528 1102 70-71 0.2346472947194016 1102 72-73 0.2627253247905301 1102 74-75 0.37814184091257985 1102 76-77 0.28863049131895835 1102 78-79 0.16180918030491398 1102 80-81 0.12163601426210136 1102 82-83 0.20226557680724966 1102 84-85 0.2011987602712395 1102 86-87 0.1732363684045204 1102 88-89 0.10459470699231233 1102 90-91 0.19284301424603711 1102 92-93 0.16833980860362985 1102 94-95 0.21641471198288187 1102 96-97 0.032304042442419245 1102 98-99 0.1686585060786605 1102 100-101 0.15288479127938714 1102 102-103 0.03944368088225758 1102 104-105 0.062441497641525956 1102 106-107 -0.0784671346739465 1102 108-109 0.025418883521467706 1102 110-111 0.19945839643111896 1102 112-113 0.32259631444419057 1102 114-115 0.36879792419273016 1102 116-117 0.37733666054551307 1102 118-119 0.45476413450901276 1102 120-121 0.37420789230991147 1102 122-123 0.45232353789859303 1102 124-125 0.7177344115278785 1103 1 0.1291390095765763 1103 2 0.5888134180691686 1103 3 0.29675943755286127 1103 4 0.20995401887200416 1103 5 0.3770374238855041 1103 6 0.09388640174960727 1103 7 0.2032387017404531 1103 8 0.23711786616982522 1103 9 0.21964038046067458 1103 10-11 0.2937064006600778 1103 12-13 0.2560902967003713 1103 14-15 0.3758237170668721 1103 16-17 0.45394682387912866 1103 18-19 0.40480302614328423 1103 20-21 0.27649121623881 1103 22-23 0.2982041923232046 1103 24-25 0.27914425539687926 1103 26-27 0.18386501150311574 1103 28-29 0.18356676377094772 1103 30-31 -0.03975795373868607 1103 32-33 0.24301282122114287 1103 34-35 0.2627474026064647 1103 36-37 0.3156078966915459 1103 38-39 0.1862211919861423 1103 40-41 0.22870449012098248 1103 42-43 0.23779511152984156 1103 44-45 0.07196134558634526 1103 46-47 0.24644540940352755 1103 48-49 0.11358426898738117 1103 50-51 0.12337284250580183 1103 52-53 0.12952068689524765 1103 54-55 0.06761368648278676 1103 56-57 0.04562501479460224 1103 58-59 0.1128040220617379 1103 60-61 -0.009501535737527433 1103 62-63 0.007096118203442359 1103 64-65 0.026527896491920444 1103 66-67 -0.009154653065500895 1103 68-69 -0.15721705783115425 1103 70-71 -0.14260914142396075 1103 72-73 -0.18330193416959162 1103 74-75 -0.12430263157606092 1103 76-77 -0.03954719671738616 1103 78-79 -0.14678370207772318 1103 80-81 -0.044733243688430235 1103 82-83 0.029592684328704877 1103 84-85 -0.006072819738854207 1103 86-87 -0.04268488348846233 1103 88-89 0.10617976504380167 1103 90-91 0.058036098546892845 1103 92-93 0.08142789542846529 1103 94-95 -0.039406842787439444 1103 96-97 0.02850293189219144 1103 98-99 0.06216557321646832 1103 100-101 0.07269296896742361 1103 102-103 0.21060597265409342 1103 104-105 0.04661615690452692 1103 106-107 0.1754425069212111 1103 108-109 0.05322933278951325 1103 110-111 0.2021476947652907 1103 112-113 0.14821468900249712 1103 114-115 0.20236556679747864 1103 116-117 0.01706785691098034 1103 118-119 0.08788755702289563 1103 120-121 0.052414606090831484 1103 122-123 0.003055491457399029 1103 124-125 -0.06740819321720437 1104 1 0.08937812449449467 1104 2 0.37191936280252236 1104 3 0.17760934068327572 1104 4 0.045273688495740316 1104 5 0.28099938982819594 1104 6 0.08210349158340335 1104 7 0.38280992637591993 1104 8 0.1646339261270029 1104 9 0.2159710626264726 1104 10-11 0.22402658012372711 1104 12-13 0.23180432749470015 1104 14-15 0.18694128615018002 1104 16-17 0.33856852266571025 1104 18-19 0.19507171177599503 1104 20-21 0.26700870374773444 1104 22-23 0.29095503355905805 1104 24-25 0.19843353856196444 1104 26-27 0.23543786762629537 1104 28-29 0.2070968953096397 1104 30-31 0.12088673882784207 1104 32-33 0.03402689280267168 1104 34-35 0.06612330757231177 1104 36-37 0.16270665268216788 1104 38-39 -0.03538607833097984 1104 40-41 -0.1217003227677651 1104 42-43 -0.12958150721187423 1104 44-45 -0.27005532617872063 1104 46-47 -0.3497422626674407 1104 48-49 -0.4400383984950267 1104 50-51 -0.4540165222336725 1104 52-53 -0.4456564824735665 1104 54-55 -0.4856174239485327 1104 56-57 -0.5046250616813879 1104 58-59 -0.7396179724319936 1104 60-61 -0.6472001183825782 1104 62-63 -0.6582852420494376 1104 64-65 -0.7721658936579701 1104 66-67 -0.794415181259648 1104 68-69 -0.6730743536402706 1104 70-71 -0.606857637396228 1104 72-73 -0.6869360730499849 1104 74-75 -0.4811449380918198 1104 76-77 -0.42656259388267515 1104 78-79 -0.4442541313629249 1104 80-81 -0.5437143286277575 1104 82-83 -0.5449097119188764 1104 84-85 -0.4943531297215209 1104 86-87 -0.5364974663374475 1104 88-89 -0.47717702092540293 1104 90-91 -0.6358134476955826 1104 92-93 -0.5830507932632827 1104 94-95 -0.5859218320807962 1104 96-97 -0.6089998722273151 1104 98-99 -0.5467477030147947 1104 100-101 -0.47760579721999363 1104 102-103 -0.4753894897707056 1104 104-105 -0.5123468287179911 1104 106-107 -0.65136130668132 1104 108-109 -0.6142630196118297 1104 110-111 -0.544976043380931 1104 112-113 -0.4663820276284838 1104 114-115 -0.5014411528925393 1104 116-117 -0.5005728589042526 1104 118-119 -0.49821140290369215 1104 120-121 -0.4692681205330089 1104 122-123 -0.33033902266516435 1104 124-125 -0.13740691861742604 1105 1 0.16254731407484968 1105 2 0.6468855881136584 1105 3 0.37577822153703977 1105 4 0.21945476034505873 1105 5 0.46166174420186223 1105 6 0.37011230387516747 1105 7 0.42681507346561887 1105 8 0.3473254786265727 1105 9 0.3412098317706054 1105 10-11 0.2926930541450119 1105 12-13 0.2584370589346747 1105 14-15 0.30620928305797435 1105 16-17 0.34700910761614523 1105 18-19 0.24597208200091814 1105 20-21 0.27315211589279187 1105 22-23 0.37362065302512804 1105 24-25 0.32003896622239125 1105 26-27 0.2678862670641635 1105 28-29 0.41779316021187896 1105 30-31 0.4054632523908168 1105 32-33 0.23261984049944573 1105 34-35 0.34913766459361284 1105 36-37 0.35079202916806906 1105 38-39 0.3957503565931617 1105 40-41 0.3534301450888506 1105 42-43 0.21858478033853856 1105 44-45 0.5045280781617265 1105 46-47 0.3601811978365461 1105 48-49 0.3204070569260864 1105 50-51 0.45381401897638796 1105 52-53 0.37205034581764096 1105 54-55 0.2354646503977662 1105 56-57 0.1860983217644474 1105 58-59 0.3263371411917433 1105 60-61 0.3484866006935192 1105 62-63 0.3943155942291483 1105 64-65 0.3662164778068018 1105 66-67 0.39032631579263466 1105 68-69 0.4803447315904954 1105 70-71 0.3898846796338731 1105 72-73 0.2799407746786535 1105 74-75 0.1915290540393606 1105 76-77 0.23492635741014922 1105 78-79 0.27396147834738827 1105 80-81 0.21598474939115775 1105 82-83 0.22024765219820353 1105 84-85 0.14989109523889965 1105 86-87 0.18955436515217272 1105 88-89 -2.0678957804776132E-4 1105 90-91 -0.06324047090441454 1105 92-93 -0.049408733340687405 1105 94-95 0.07094782849284087 1105 96-97 0.04658993139787526 1105 98-99 0.016534085327194248 1105 100-101 -0.00729714472511489 1105 102-103 0.120582492673865 1105 104-105 0.1920684554710128 1105 106-107 0.006344632477315315 1105 108-109 -0.04715499741315554 1105 110-111 0.06967982526455074 1105 112-113 -0.024276660478470546 1105 114-115 0.12036235374755222 1105 116-117 0.18339385789961327 1105 118-119 0.17881563413609314 1105 120-121 0.1929076856756069 1105 122-123 0.18965954484345815 1105 124-125 0.41801073906159303 1106 1 0.1600940657563541 1106 2 0.5158880569134716 1106 3 0.20244563337950439 1106 4 0.06795161116734505 1106 5 0.24558878825147445 1106 6 0.18013038248797386 1106 7 0.2094907081609989 1106 8 0.28121898976533544 1106 9 0.13931935317334876 1106 10-11 0.20563457080454128 1106 12-13 0.23109631596200586 1106 14-15 0.08826553087104116 1106 16-17 0.18113783511508075 1106 18-19 0.16006586723477767 1106 20-21 0.1193002050028582 1106 22-23 0.07366366262817792 1106 24-25 0.08299457642416286 1106 26-27 0.2394027322093777 1106 28-29 -0.008724568492297635 1106 30-31 0.022402961189079917 1106 32-33 -0.019236376210635342 1106 34-35 0.002362169861683583 1106 36-37 0.11676560134644376 1106 38-39 0.021317500172337134 1106 40-41 0.07455320601183502 1106 42-43 0.18114382742069068 1106 44-45 0.207517124077512 1106 46-47 0.1444690851659729 1106 48-49 0.19012319354597196 1106 50-51 0.11906104475299628 1106 52-53 -0.0023312392684786687 1106 54-55 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-0.6125612080494989 2216 98-99 -0.6689204326144349 2216 100-101 -0.8543332700996302 2216 102-103 -0.7476811993575687 2216 104-105 -0.6239377789555327 2216 106-107 -0.4831872306881202 2216 108-109 -0.4603348071521758 2216 110-111 -0.5579324801618242 2216 112-113 -0.4608232118721389 2216 114-115 -0.6129186897622887 2216 116-117 -0.46652543754965947 2216 118-119 -0.4839127345222991 2216 120-121 -0.4351437029470695 2216 122-123 -0.5957783277845863 2216 124-125 -0.8448061524008779 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45.0 3 56.0 4 166.0 5 400.0 6 1109.0 7 1905.0 8 2913.0 9 4886.0 10 7946.0 11 9819.0 12 10425.0 13 13172.0 14 18334.0 15 12903.0 16 5700.0 17 4952.0 18 4842.0 19 4541.0 20 4722.0 21 4855.0 22 5472.0 23 5964.0 24 7177.0 25 8481.0 26 10431.0 27 12636.0 28 15340.0 29 18899.0 30 23365.0 31 28251.0 32 35122.0 33 44194.0 34 59101.0 35 85868.0 36 131705.0 37 202389.0 38 79085.0 39 210.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.611691540886 18.01769292790329 12.128282184721384 20.242333346489325 2 19.097858069343694 22.86085846263764 36.80645317604581 21.23483029197286 3 17.378124366111475 30.133088417588855 32.49295679415808 19.995830422141587 4 10.887431666893928 17.442226056226133 37.05837740497036 34.611964871909585 5 9.632274920475266 41.96357592371038 35.14705733110367 13.257091824710685 6 27.38327730704173 39.11397697268704 18.125021833913504 15.37772388635772 7 24.399553292216083 35.672726821962605 23.559891410791984 16.367828475029327 8 27.86784932289513 32.30359901778379 20.816312271729956 19.012239387591126 9 27.40040636434632 10.968456615591274 20.88708232427785 40.74405469578456 10-11 23.84471833406395 26.422134348154852 29.394420209583032 20.33872710819817 12-13 26.148463850364163 26.158493364180664 29.83571881750905 17.857323967946126 14-15 22.879969257849787 26.269888582243706 30.04949396031693 20.800648199589578 16-17 19.536309657294893 27.860468051490848 31.039485857822065 21.563736433392194 18-19 18.52913235690194 24.365971324605777 36.954701531811025 20.150194786681254 20-21 19.778426628471873 23.409505049127713 38.153960925464936 18.658107396935478 22-23 19.475062008314353 23.706897037461925 38.04442511164877 18.773615842574948 24-25 19.15625869834941 24.099344024719933 37.96599205978041 18.778405217150244 26-27 18.922086454409097 24.015332760110933 38.08978330615598 18.972797479323987 28-29 18.92287529257444 23.00533818055604 38.836474975236115 19.2353115516334 30-31 19.0983917843632 23.44077684782523 38.387006257740474 19.0738251100711 32-33 18.746344580287385 24.104415127211425 37.90750534437857 19.24173494812262 34-35 19.178818544479252 24.038010773287656 37.71963533097433 19.06353535125876 36-37 19.68145587971796 23.726561646012254 36.60485180548152 19.987130668788268 38-39 19.48165444155329 24.078045394255682 36.936952673090815 19.503347491100214 40-41 19.36362135521346 24.05788723101308 36.94672127683671 19.631770136936748 42-43 19.622453544836883 24.11983397963174 35.80510074253883 20.452611732992548 44-45 19.556935026792058 24.511873990261428 35.37622429481013 20.55496668813638 46-47 20.154391809649177 24.680036245277744 34.19830990614096 20.967262038932116 48-49 20.391828482201372 25.151428234934798 33.141215246452305 21.315528036411525 50-51 20.488081405504946 25.189420620278575 32.70195004908088 21.620547925135604 52-53 20.511787441192396 25.43869386687914 32.53172609927743 21.517792592651027 54-55 20.495616570816132 25.628494374627387 32.38807013035898 21.487818924197498 56-57 20.76199771109925 25.465457715347977 32.01778828626023 21.754756287292544 58-59 20.64608466830827 24.739475626729178 32.613864956696666 22.000574748265894 60-61 21.09414751100881 25.415630115257944 32.369873472642084 21.12034890109116 62-63 20.906358608192356 25.360844027929915 32.84869568745182 20.88410167642591 64-65 21.00460917778579 25.20668049044356 33.0151235124414 20.773586819329246 66-67 21.05985886128873 25.52521316182719 32.45508556900628 20.9598424078778 68-69 21.040176071059502 25.50055275764648 32.53054817105398 20.928723000240034 70-71 21.151660527625125 25.069208607392657 32.672773590232076 21.106357274750142 72-73 21.08025049613516 25.80883272722765 31.832043175310105 21.278873601327085 74-75 20.589516341239268 25.93400847333952 31.962937732392803 21.513537453028412 76-77 20.090863655495685 25.898339312589343 32.75700705509011 21.253789976824866 78-79 20.29128494774214 25.675176939716255 32.40009759082511 21.633440521716494 80-81 19.838862174701397 26.3200984474468 31.87061418089493 21.970425196956874 82-83 20.510188155336195 25.585530864225355 31.6824218752201 22.221859105218346 84-85 19.800412000297506 25.513929802181256 31.886467773598177 22.79919042392306 86-87 19.9287562431258 25.86896648095058 31.840448693676638 22.361828582246986 88-89 20.20896617357952 25.61314718744242 31.8060647859373 22.37182185304076 90-91 20.17594818808752 25.88245966844478 31.675970445031826 22.265621698435872 92-93 20.09496532756953 25.937750210874416 31.267618648904943 22.699665812651112 94-95 19.897167746170556 25.421485461209702 31.692900630234938 22.988446162384808 96-97 20.416772785975574 25.662128141037634 31.30940333526414 22.611695737722652 98-99 20.223616231588117 25.72471301381557 31.102354884132126 22.949315870464186 100-101 20.225384491856577 25.675012466579705 31.150108888882627 22.949494152681087 102-103 20.5974611535304 25.32158177268483 30.931577135393688 23.14937993839108 104-105 20.024758444579174 25.77836402689283 31.047393770833942 23.14948375769405 106-107 20.10709132062204 25.6370062887837 31.26530147677387 22.99060091382039 108-109 20.56529747620365 25.74682475868391 30.169257928031435 23.518619837081005 110-111 20.31409618181337 26.218049599294098 30.004287944789752 23.463566274102785 112-113 20.00211883867354 25.831686443433487 30.71104506645884 23.45514965143413 114-115 19.94945908598042 26.21161809824595 30.081086381680834 23.7578364340928 116-117 19.869320974017484 26.264410310049907 30.06930699765107 23.796961718281537 118-119 19.548878959650214 25.94022057336 30.16950145867391 24.34139900831588 120-121 19.604979260594117 26.209300923398025 29.92717963802256 24.258540177985292 122-123 19.71298838294362 26.67245941693827 29.333856258649682 24.280695941468426 124-125 19.743803436502798 26.47368246074021 28.898550871635187 24.883963231121804 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 578.0 1 488.5 2 1376.0 3 2491.0 4 1785.5 5 1422.0 6 2404.5 7 5973.5 8 9249.0 9 9513.0 10 6883.0 11 3494.0 12 2715.0 13 2603.5 14 2570.0 15 2591.5 16 2615.0 17 2678.0 18 2813.5 19 2944.5 20 3013.5 21 3166.0 22 3463.0 23 3839.5 24 4234.0 25 4701.5 26 5386.5 27 6107.0 28 7005.0 29 8134.5 30 10441.5 31 12562.5 32 14825.5 33 18659.0 34 22160.5 35 24492.5 36 27659.0 37 30427.5 38 30561.5 39 31070.5 40 29808.0 41 28503.5 42 28217.5 43 27583.5 44 28853.5 45 30582.5 46 29283.0 47 27014.0 48 26569.0 49 25417.0 50 25300.0 51 23597.5 52 21149.5 53 21168.0 54 20782.5 55 19221.0 56 17707.5 57 16544.0 58 15567.0 59 14895.0 60 13608.0 61 12061.0 62 10450.0 63 8107.5 64 6124.0 65 5027.0 66 3767.5 67 2992.0 68 2256.5 69 1327.5 70 943.5 71 833.0 72 810.5 73 807.5 74 645.5 75 428.0 76 263.0 77 152.5 78 94.0 79 45.5 80 25.0 81 13.0 82 8.0 83 5.5 84 4.0 85 4.0 86 3.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06603702355583453 2 4.507646659101333E-4 3 1.1269116647753332E-4 4 0.0 5 0.06198014156264332 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 1.1269116647753332E-4 36-37 0.0 38-39 0.0 40-41 5.634558323876666E-5 42-43 0.0022538233295506666 44-45 0.009522403567351567 46-47 0.010480278482410598 48-49 0.008958947734963899 50-51 0.007888381653427333 52-53 0.006705124405413232 54-55 0.0069868523216070665 56-57 0.0062543597395031 58-59 0.0040568819931912 60-61 0.002648242412222033 62-63 0.0019157498301180665 64-65 0.0023665144960282 66-67 0.0030426614948934 68-69 0.0013522939977303999 70-71 0.0030990070781321665 72-73 0.002479205662505733 74-75 0.0010705660815365666 76-77 8.451837485814998E-4 78-79 2.817279161938333E-4 80-81 4.507646659101333E-4 82-83 2.817279161938333E-4 84-85 3.3807349943259997E-4 86-87 5.634558323876666E-4 88-89 0.0014086395809691666 90-91 0.0019720954133568332 92-93 5.071102491489E-4 94-95 0.0015213307474467 96-97 0.0016903674971629997 98-99 0.0014086395809691666 100-101 9.578749150590333E-4 102-103 0.0010705660815365666 104-105 7.888381653427334E-4 106-107 8.451837485814998E-4 108-109 8.451837485814998E-4 110-111 0.0010705660815365666 112-113 0.011550844563947166 114-115 0.020171718799478468 116-117 0.019044807134703134 118-119 0.011550844563947166 120-121 0.006817815571890767 122-123 0.0073249258210396654 124-125 0.0061416685730255665 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 887381.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.960666235448784 #Duplication Level Percentage of deduplicated Percentage of total 1 54.48938571028123 13.056019843786812 2 12.243821549389374 5.867402431826283 3 8.405255544385252 6.041865681680112 4 5.9646863523548435 5.716714355516435 5 4.319559019663453 5.1749755977239165 6 3.0669653902867022 4.409192044339956 7 2.122724376175276 3.5603323201170918 8 1.5236954295582983 2.9207006105700124 9 1.1443095893464525 2.4676578126318427 >10 6.0123697396028914 25.83785767300436 >50 0.46054191712067555 7.662507037431753 >100 0.23355384059716464 9.92072660416355 >500 0.007034754234854351 1.044606382877095 >1k 0.004689836156569567 2.1312397437227824 >5k 0.0 0.0 >10k+ 0.0014069508469708703 4.188201860608026 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13310 1.4999194258159685 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12037 1.3564635708900687 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11924 1.3437294690781074 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3801 0.42833912378110417 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3178 0.3581325270656009 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2941 0.3314247206104255 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1955 0.22031123046357765 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1698 0.1913496006788516 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1243 0.14007511993157393 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1067 0.12024147463152805 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1054 0.11877648946732013 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1015 0.11438153397469633 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1014 0.11426884280821879 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1269116647753332E-4 0.0 12-13 0.0 0.0 0.0 1.1269116647753332E-4 0.0 14-15 0.0 0.0 0.0 2.2538233295506664E-4 0.0 16-17 0.0 0.0 0.0 2.2538233295506664E-4 0.0 18-19 0.0 0.0 0.0 3.3807349943259997E-4 0.0 20-21 0.0 0.0 0.0 3.944190826713666E-4 0.0 22-23 0.0 0.0 0.0 9.578749150590332E-4 0.0 24-25 0.0 0.0 0.0 0.0018030586636405333 0.0 26-27 0.0 0.0 0.0 0.002648242412222033 0.0 28-29 0.0 0.0 0.0 0.004169573159668732 0.0 30-31 0.0 0.0 0.0 0.009353366817635265 0.0 32-33 0.0 0.0 0.0 0.021974777463118995 0.0 34-35 0.0 0.0 0.0 0.0349906071912741 0.0 36-37 0.0 0.0 0.0 0.05414810549245476 0.0 38-39 0.0 0.0 0.0 0.08547624977320903 0.0 40-41 0.0 0.0 0.0 0.12300240821022762 0.0 42-43 0.0 0.0 0.0 0.16571236030521275 0.0 44-45 0.0 0.0 0.0 0.2185645173831759 0.0 46-47 0.0 0.0 0.0 0.28189695294354966 0.0 48-49 0.0 0.0 0.0 0.35052587332836743 0.0 50-51 0.0 0.0 0.0 0.42045074212767686 0.0 52-53 0.0 0.0 0.0 0.494545184086655 0.0 54-55 0.0 0.0 0.0 0.5755701327840015 0.0 56-57 0.0 0.0 0.0 0.6919237621720546 0.0 58-59 0.0 0.0 0.0 0.8254627944479316 0.0 60-61 0.0 0.0 0.0 0.952634775817828 0.0 62-63 0.0 0.0 0.0 1.071411265285148 0.0 64-65 0.0 0.0 0.0 1.20382338589625 0.0 66-67 0.0 0.0 0.0 1.3833404140949603 0.0 68-69 0.0 0.0 0.0 1.5966647922369308 0.0 70-71 0.0 0.0 0.0 1.8125810672078848 0.0 72-73 0.0 0.0 0.0 2.021679526606948 0.0 74-75 0.0 0.0 0.0 2.2494847196412815 0.0 76-77 0.0 0.0 0.0 2.5046738661296555 0.0 78-79 0.0 0.0 0.0 2.8308584474988763 0.0 80-81 0.0 0.0 0.0 3.156085153953037 0.0 82-83 0.0 0.0 0.0 3.4841854851523753 0.0 84-85 0.0 0.0 0.0 3.8230478227503184 0.0 86-87 0.0 0.0 0.0 4.194759635376462 0.0 88-89 0.0 0.0 0.0 4.616900745001301 0.0 90-91 0.0 0.0 0.0 5.08248429930323 0.0 92-93 0.0 0.0 0.0 5.5386581412042855 0.0 94-95 0.0 0.0 0.0 6.015229084237774 0.0 96-97 0.0 0.0 0.0 6.536425729196365 0.0 98-99 0.0 0.0 0.0 7.111263369398263 0.0 100-101 0.0 0.0 0.0 7.743742541253418 0.0 102-103 0.0 0.0 0.0 8.372502904614816 0.0 104-105 0.0 0.0 0.0 9.01146181854243 0.0 106-107 0.0 0.0 0.0 9.655717217294487 0.0 108-109 0.0 0.0 0.0 10.320257026012502 0.0 110-111 0.0 0.0 0.0 11.082049311400628 0.0 112-113 0.0 0.0 0.0 11.822655657490976 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5130 0.0 76.982346 1 TATAACG 95 0.0 62.66167 2 CACGCAG 230 0.0 54.35219 5 ATCACGC 125 1.0913936E-11 47.62287 3 TATTCGC 25 5.328176E-4 47.60945 44-45 GTATCAA 8335 0.0 47.35147 2 TATCACG 130 1.8189894E-11 45.79122 2 GTACATG 8195 0.0 43.247704 1 ACATGGG 8330 0.0 42.23457 3 TCACGCA 145 5.820766E-11 41.054195 4 TACATGG 8610 0.0 40.999363 2 GGGTATG 340 0.0 40.244377 7 ATGGGAT 2160 0.0 39.961315 5 CATGGGG 2015 0.0 38.99639 4 CATGGGA 6080 0.0 38.86982 4 CGGGAGT 510 0.0 38.518494 4 ATGGGTA 470 0.0 37.99697 5 GTCGGGA 550 0.0 35.71715 2 ATGGGAG 1935 0.0 35.68639 5 TCGGGAG 575 0.0 35.19951 3 >>END_MODULE