FastQCFastQC Report
Thu 26 May 2016
SRR936241_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936241_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900362
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15230.16915418465017404No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12990.14427530260050958No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG11850.1316137287002339No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT26150.046.412735
TACATGG117550.046.310252
GTACATG119700.046.223991
TACCTGG6650.043.8382382
ACATGGG124300.043.1731833
ATGGGAG25500.041.529675
CATGGGA93350.040.5341874
CATGGGG29350.038.9199034
TGGGATT12100.031.46836
CATGGGT8150.029.1998774
ATGGGGG14300.029.1233065
GGTATCA39600.028.6957021
TGGGATA7550.028.3683596
CGGGAGT4200.028.3308334
GTCGGGA4200.028.3308332
AGTACAT73100.028.3230842
ATGGGAC14450.027.58585
GAACAAA15550.027.5474111
ATGGGAA41550.027.2057825
GAGTACA81250.027.1662221
Produced by FastQC (version 0.11.5)