##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936241_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 900362 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.026878077928654 31.0 30.0 33.0 22.0 34.0 2 28.96854487417283 31.0 30.0 34.0 20.0 34.0 3 29.18074729941957 31.0 30.0 34.0 22.0 34.0 4 32.49831179014663 35.0 33.0 37.0 20.0 37.0 5 32.892274440724954 35.0 33.0 37.0 26.0 37.0 6 32.7287402178235 36.0 35.0 37.0 23.0 37.0 7 32.608931740788705 35.0 33.0 37.0 23.0 37.0 8 32.493118323518765 35.0 33.0 37.0 21.0 37.0 9 33.75411001352789 38.0 34.0 39.0 17.0 39.0 10-11 33.707758657073484 38.0 34.0 39.0 17.5 39.0 12-13 33.700288328472325 38.0 34.0 39.0 18.0 39.0 14-15 34.74251912008725 39.0 34.0 41.0 18.0 41.0 16-17 34.808609203853564 39.0 34.0 41.0 20.0 41.0 18-19 34.863599863166144 39.0 34.0 41.0 21.5 41.0 20-21 35.03720947796553 39.0 34.0 41.0 23.5 41.0 22-23 34.97194739449243 39.0 34.0 41.0 24.0 41.0 24-25 34.98047007759101 39.0 34.0 41.0 24.5 41.0 26-27 35.101764623562524 39.0 34.0 41.0 26.0 41.0 28-29 34.98846963776792 39.0 34.0 41.0 25.5 41.0 30-31 34.888534278434676 39.0 34.0 41.0 25.0 41.0 32-33 34.710561418629396 39.0 33.0 41.0 24.5 41.0 34-35 34.6384693045686 38.5 33.0 40.0 24.5 41.0 36-37 34.474319773602176 38.0 33.0 40.0 24.0 41.0 38-39 34.18958152387595 38.0 33.0 40.0 23.0 41.0 40-41 34.2530471077189 38.0 33.0 40.0 23.5 41.0 42-43 34.11935532596889 38.0 33.0 40.0 22.5 41.0 44-45 33.93963539109825 38.0 33.0 40.0 21.5 41.0 46-47 33.87134119387535 38.0 33.0 40.0 22.0 41.0 48-49 33.724513584536 38.0 32.5 40.0 20.5 41.0 50-51 33.57189386046946 38.0 32.0 40.0 19.5 41.0 52-53 33.38911071324645 38.0 32.0 40.0 18.0 41.0 54-55 33.18164638223293 38.0 32.0 40.0 16.0 41.0 56-57 32.9299853836568 37.0 31.0 40.0 13.5 41.0 58-59 32.650555554321485 37.0 31.0 40.0 9.5 41.0 60-61 32.36786148238153 36.5 31.0 40.0 7.5 41.0 62-63 32.084454363911405 36.0 31.0 40.0 6.5 41.0 64-65 31.77430244723789 36.0 30.5 39.0 2.0 41.0 66-67 31.463108727378543 35.0 30.0 39.0 2.0 41.0 68-69 31.171580430982203 35.0 30.0 39.0 2.0 40.0 70-71 30.808811900102405 35.0 30.0 38.0 2.0 40.0 72-73 30.492211466054762 35.0 30.0 37.0 2.0 40.0 74-75 30.158024772258273 34.5 29.0 37.0 2.0 39.0 76-77 29.815604168101274 34.0 29.0 36.0 2.0 39.0 78-79 29.48817919903328 34.0 29.0 36.0 2.0 38.5 80-81 29.18672545042994 34.0 29.0 35.5 2.0 37.0 82-83 28.89919721178815 34.0 29.0 35.0 2.0 37.0 84-85 28.94533309935337 34.0 29.0 35.0 2.0 36.5 86-87 28.82942305428261 34.0 29.0 35.0 2.0 36.0 88-89 28.646774297449248 34.0 29.0 35.0 2.0 36.0 90-91 28.481607953245472 34.0 29.0 35.0 2.0 36.0 92-93 28.346063027982076 34.0 29.0 35.0 2.0 35.0 94-95 28.22190907657142 34.0 29.0 35.0 2.0 35.0 96-97 28.112388683662793 34.0 29.0 35.0 2.0 35.0 98-99 27.990563795451163 34.0 28.0 35.0 2.0 35.0 100-101 27.84703319331558 34.0 28.0 35.0 2.0 35.0 102-103 26.850397951046354 32.5 25.5 34.0 2.0 35.0 104-105 27.49288786066049 34.0 27.0 35.0 2.0 35.0 106-107 27.582726725472643 34.0 27.0 35.0 2.0 35.0 108-109 27.49953463162595 34.0 27.0 35.0 2.0 35.0 110-111 27.364679428940804 34.0 27.0 35.0 2.0 35.0 112-113 27.26993531490667 34.0 27.0 35.0 2.0 35.0 114-115 27.114719412858385 34.0 26.5 35.0 2.0 35.0 116-117 26.950999709005934 34.0 25.0 35.0 2.0 35.0 118-119 26.703174389856525 34.0 25.0 35.0 2.0 35.0 120-121 26.456877344890167 34.0 25.0 35.0 2.0 35.0 122-123 26.088089568418035 34.0 24.5 35.0 2.0 35.0 124-125 24.054505854311934 31.5 12.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3425257107587534 1101 2 0.3335071752932244 1101 3 0.21753432205767353 1101 4 0.5259912451222206 1101 5 0.2693585980079618 1101 6 0.26001703348919136 1101 7 0.41011994171156374 1101 8 0.48379451281034136 1101 9 0.38384777934154357 1101 10-11 0.5035602417810807 1101 12-13 0.4717624454585092 1101 14-15 0.5909224487998088 1101 16-17 0.4892984810126393 1101 18-19 0.5322115089627957 1101 20-21 0.5504762595580814 1101 22-23 0.6993673522863233 1101 24-25 0.8313526543650696 1101 26-27 0.6770080818602366 1101 28-29 0.8827299277384313 1101 30-31 0.8922645190350096 1101 32-33 0.8846783747918039 1101 34-35 0.8712588777199244 1101 36-37 0.7451998946411393 1101 38-39 0.8967928433313759 1101 40-41 0.7586188644716501 1101 42-43 0.8877876612556506 1101 44-45 0.9037086972873922 1101 46-47 0.8431583794064963 1101 48-49 0.7797674292165837 1101 50-51 0.8501707611729614 1101 52-53 0.8348663226717008 1101 54-55 0.7484589295192592 1101 56-57 0.8077740712854151 1101 58-59 0.9129777359557778 1101 60-61 1.01743995959054 1101 62-63 0.8197013480686408 1101 64-65 0.9132774987794967 1101 66-67 0.9306890927134859 1101 68-69 0.8750361300297698 1101 70-71 0.8701823411451919 1101 72-73 0.883376190433566 1101 74-75 0.8498528900524285 1101 76-77 0.8669514252029806 1101 78-79 0.8027643541731315 1101 80-81 0.8586252139904822 1101 82-83 0.8037721058422491 1101 84-85 0.5406671787893877 1101 86-87 0.8147888970749051 1101 88-89 0.77103630008531 1101 90-91 0.6945109013138477 1101 92-93 0.7399504100212759 1101 94-95 1.014820232026704 1101 96-97 0.9531977379269811 1101 98-99 1.0242646282575372 1101 100-101 0.9943293933627331 1101 102-103 1.0487155488888256 1101 104-105 1.0275421635698017 1101 106-107 0.9284169015369237 1101 108-109 0.8801793588441669 1101 110-111 0.9410820734310619 1101 112-113 0.9348808502456691 1101 114-115 0.8312449000845064 1101 116-117 0.9737408644402201 1101 118-119 0.9838605191421514 1101 120-121 1.1944463292928837 1101 122-123 1.1074966875995074 1101 124-125 0.929017360347526 1102 1 0.5916131320721227 1102 2 0.6089616022310551 1102 3 0.5205663181838673 1102 4 0.5842752247102538 1102 5 0.5838909479912004 1102 6 0.4738304949560259 1102 7 0.5306268884840151 1102 8 0.5819013776369459 1102 9 0.7990524577041214 1102 10-11 0.8680399788094277 1102 12-13 0.7774297453430421 1102 14-15 0.8034461944697142 1102 16-17 0.6640549714957444 1102 18-19 0.7633034372974308 1102 20-21 0.8084520110386961 1102 22-23 0.8896493204764298 1102 24-25 0.9885652851990514 1102 26-27 0.8005623896041172 1102 28-29 0.7196786557154908 1102 30-31 0.7965252371785567 1102 32-33 0.9075231060466891 1102 34-35 0.7783271721289822 1102 36-37 0.8497460264249526 1102 38-39 0.8683771916756982 1102 40-41 0.6271167413293952 1102 42-43 0.8796955094323522 1102 44-45 0.9100851080595405 1102 46-47 0.6486720308386822 1102 48-49 0.6232043122947744 1102 50-51 0.3901868709803864 1102 52-53 0.6377446703455618 1102 54-55 0.6429070987746641 1102 56-57 0.6565351060379001 1102 58-59 0.7409380294708754 1102 60-61 0.7492233059607116 1102 62-63 0.749409509129471 1102 64-65 0.729323053217616 1102 66-67 0.594832199950794 1102 68-69 0.5806037912209661 1102 70-71 0.5489755955125339 1102 72-73 0.4715866981253001 1102 74-75 0.4685020873556667 1102 76-77 0.3574764373086161 1102 78-79 0.42451911261914077 1102 80-81 0.534273331098543 1102 82-83 0.5431028504319606 1102 84-85 0.5712571888463778 1102 86-87 0.5552976131325664 1102 88-89 0.4961964669568424 1102 90-91 0.3688972387072269 1102 92-93 0.5525761223913719 1102 94-95 0.47230384285845517 1102 96-97 0.6953802909124214 1102 98-99 0.6524470136264817 1102 100-101 0.6483549828140696 1102 102-103 0.5871230059603789 1102 104-105 0.5706894425953912 1102 106-107 0.320960761186047 1102 108-109 0.5081778316716736 1102 110-111 0.5725120283608476 1102 112-113 0.5555949896280943 1102 114-115 0.45422651814239146 1102 116-117 0.326163853973501 1102 118-119 0.367562608761105 1102 120-121 0.35273449929325196 1102 122-123 0.6080158331278724 1102 124-125 0.6617063013837701 1103 1 0.26279547118131674 1103 2 0.3433496578436852 1103 3 0.33273900042024707 1103 4 0.4184737955843971 1103 5 0.22524427995476515 1103 6 0.3508206554473148 1103 7 0.16966531050990596 1103 8 0.23818519381391923 1103 9 0.3596259344066297 1103 10-11 0.5220591099191694 1103 12-13 0.40914218513027123 1103 14-15 0.38280605566643544 1103 16-17 0.2142866907843768 1103 18-19 0.39387440275642405 1103 20-21 0.2795886911747587 1103 22-23 0.3233250961082419 1103 24-25 0.19076833065255272 1103 26-27 0.19783198301096405 1103 28-29 0.3048554700889383 1103 30-31 0.28605106874260855 1103 32-33 0.3149061620017619 1103 34-35 0.27040762323963463 1103 36-37 0.1602064971689643 1103 38-39 0.20274926664773574 1103 40-41 0.34916545945372945 1103 42-43 0.3896080724988167 1103 44-45 0.3691609704433958 1103 46-47 0.1767888264919506 1103 48-49 0.08339881952030481 1103 50-51 0.10201711091828258 1103 52-53 0.07535396651249471 1103 54-55 0.2807264262179885 1103 56-57 0.27499959477155045 1103 58-59 0.1872750383515509 1103 60-61 0.16640477754941685 1103 62-63 0.07239423620295327 1103 64-65 0.06864912677422197 1103 66-67 0.05790093953483577 1103 68-69 0.06462771652288524 1103 70-71 0.2875163347313112 1103 72-73 0.09368792406873183 1103 74-75 0.177588223008474 1103 76-77 0.0654941529901869 1103 78-79 -0.041878166106062764 1103 80-81 -0.04752928516061772 1103 82-83 0.14026286230329177 1103 84-85 0.13309313615592444 1103 86-87 0.1133575633642856 1103 88-89 0.2722483355503158 1103 90-91 0.27095874134403175 1103 92-93 0.1524551261125815 1103 94-95 0.1848235332906185 1103 96-97 0.18389477622164563 1103 98-99 0.035213034218678274 1103 100-101 0.1884389953246277 1103 102-103 0.14610755039797496 1103 104-105 0.24258177873674924 1103 106-107 0.14425825222547317 1103 108-109 0.07710918340556816 1103 110-111 0.16798265822638214 1103 112-113 0.16831557954202836 1103 114-115 0.15810068201244576 1103 116-117 0.3219015888318424 1103 118-119 0.36185523711989376 1103 120-121 0.3987450465160478 1103 122-123 0.4367010829966027 1103 124-125 0.30749311963822734 1104 1 0.37796132231339996 1104 2 0.516796470301653 1104 3 0.4212604478201989 1104 4 0.4721877632567626 1104 5 0.4125167870343276 1104 6 0.4178686779370011 1104 7 0.4033838647590571 1104 8 0.47755399839225277 1104 9 0.6036694398911919 1104 10-11 0.7527690759321146 1104 12-13 0.6136488833360971 1104 14-15 0.6660372217134025 1104 16-17 0.6622421855291378 1104 18-19 0.5347797105595262 1104 20-21 0.6140748528541664 1104 22-23 0.6947659254025496 1104 24-25 0.6208287034021112 1104 26-27 0.6073841733590584 1104 28-29 0.5864442293683751 1104 30-31 0.6255270652279563 1104 32-33 0.734130102901922 1104 34-35 0.6565502348570789 1104 36-37 0.7867230951658186 1104 38-39 1.0845238080544704 1104 40-41 0.7577851317453934 1104 42-43 0.9683294482657132 1104 44-45 0.9810587567759157 1104 46-47 0.8134976742047186 1104 48-49 0.8769134514732855 1104 50-51 0.756752493781498 1104 52-53 0.8890518553988045 1104 54-55 0.8359606637253663 1104 56-57 0.8784248400116823 1104 58-59 0.8097567371371426 1104 60-61 0.9267254771827496 1104 62-63 0.9078647081823235 1104 64-65 0.9578124372519774 1104 66-67 0.9497559006391185 1104 68-69 0.7590802776622141 1104 70-71 0.7184705746092774 1104 72-73 0.7930695092205902 1104 74-75 0.7824989522484174 1104 76-77 0.7024745878904319 1104 78-79 0.5701085173608753 1104 80-81 0.6365369986041891 1104 82-83 0.6881959395098107 1104 84-85 0.727130070982728 1104 86-87 0.6937216035715608 1104 88-89 0.6127805330617129 1104 90-91 0.5675260235911459 1104 92-93 0.653913554462779 1104 94-95 0.6672966258029014 1104 96-97 0.6480725807974004 1104 98-99 0.7412356218210086 1104 100-101 0.668052176837115 1104 102-103 0.6967657757019623 1104 104-105 0.71768104720352 1104 106-107 0.6678734538931295 1104 108-109 0.6398777751461253 1104 110-111 0.7284347710149426 1104 112-113 0.7371348452259276 1104 114-115 0.5988026859342241 1104 116-117 0.6303091963651681 1104 118-119 0.722536678747975 1104 120-121 0.8547739471032827 1104 122-123 0.8540958043105249 1104 124-125 0.7736640237349697 1105 1 0.1305374066651872 1105 2 0.20594402984093918 1105 3 0.0958595677961327 1105 4 0.26958338149151473 1105 5 0.20548526218613716 1105 6 0.14799140203466976 1105 7 0.19971182923178077 1105 8 0.15938552935880068 1105 9 0.37399335604927586 1105 10-11 0.44124998919927094 1105 12-13 0.27456199753018495 1105 14-15 0.1982106160263868 1105 16-17 0.20584849967632124 1105 18-19 0.16674864968694436 1105 20-21 0.16947277567106056 1105 22-23 -0.1780247094282501 1105 24-25 0.15021696940799245 1105 26-27 0.24148332137746564 1105 28-29 0.14424386328652616 1105 30-31 0.3891243547946175 1105 32-33 0.28616369269649766 1105 34-35 0.4969385467506555 1105 36-37 0.47381131681359534 1105 38-39 0.4401205775605703 1105 40-41 0.44600066116195336 1105 42-43 0.6530794367937887 1105 44-45 0.761847617282406 1105 46-47 0.546925332249593 1105 48-49 0.6055295295027676 1105 50-51 0.5766529684642379 1105 52-53 0.6221967942406934 1105 54-55 0.6641550848010738 1105 56-57 0.6485868745702206 1105 58-59 0.7343352076491882 1105 60-61 0.7739240332498696 1105 62-63 0.6469347684991504 1105 64-65 0.4700866315404859 1105 66-67 0.6077141020453212 1105 68-69 0.5992460342228796 1105 70-71 0.6441014029841874 1105 72-73 0.599167219424487 1105 74-75 0.6046339028681551 1105 76-77 0.49260465401970066 1105 78-79 0.45411436039436026 1105 80-81 0.5084914576137258 1105 82-83 0.5057551218930136 1105 84-85 0.4762525962337101 1105 86-87 0.5259824394414601 1105 88-89 0.5883468637284572 1105 90-91 0.514686339910341 1105 92-93 0.35415191555816605 1105 94-95 0.3457630589521763 1105 96-97 0.361276000960455 1105 98-99 0.4357163515373692 1105 100-101 0.4277778193923467 1105 102-103 0.2681848782405716 1105 104-105 0.3732650701269762 1105 106-107 0.49802931910566883 1105 108-109 0.4911753772589975 1105 110-111 0.6007249750112535 1105 112-113 0.4725861507309226 1105 114-115 0.59014903097912 1105 116-117 0.6327305372036882 1105 118-119 0.5981817832954448 1105 120-121 0.610439548155643 1105 122-123 0.5509846184194629 1105 124-125 0.643369730634948 1106 1 -0.24765420729593046 1106 2 -0.265119500907705 1106 3 -0.0705873055062014 1106 4 -0.009504351447091608 1106 5 -0.2220307737582985 1106 6 -0.19158735227023982 1106 7 -0.37757120581055403 1106 8 -0.14251336569360262 1106 9 -0.22523113720491494 1106 10-11 0.08676540713062053 1106 12-13 -0.09984004988737638 1106 14-15 -0.16872020139560107 1106 16-17 -0.26690983228643006 1106 18-19 -0.17534202878177751 1106 20-21 -0.10824810170816335 1106 22-23 -0.23480516027876774 1106 24-25 -4.0425400667208535 1106 26-27 -0.9689351831305331 1106 28-29 -0.6194802636453858 1106 30-31 -0.45813570411598903 1106 32-33 -5.406094198184327 1106 34-35 -1.3201549975045594 1106 36-37 -0.41526231147147286 1106 38-39 -0.2289715412773674 1106 40-41 -0.7623803937465112 1106 42-43 -0.2555149323095449 1106 44-45 -0.27950294067375836 1106 46-47 -0.30011493329667616 1106 48-49 -0.31537276960559524 1106 50-51 -0.2266254912876633 1106 52-53 -0.2429709414889345 1106 54-55 -0.2964455840485698 1106 56-57 -0.18814278955959907 1106 58-59 0.01713849508010412 1106 60-61 -0.005234472155258629 1106 62-63 -0.006295845078199136 1106 64-65 0.1503194330835207 1106 66-67 0.05685262170461769 1106 68-69 -0.003808395784645313 1106 70-71 -0.01234412464448198 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-0.6688542421551098 2216 114-115 -0.7361145773839013 2216 116-117 -0.5869506687066348 2216 118-119 -0.5049926734345753 2216 120-121 -0.7191610366313803 2216 122-123 -0.4779069830737299 2216 124-125 -0.5027383130253646 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31795.0 3 4685.0 4 3388.0 5 3427.0 6 4979.0 7 5483.0 8 5299.0 9 5256.0 10 6037.0 11 6665.0 12 6399.0 13 5954.0 14 5738.0 15 5601.0 16 5032.0 17 4840.0 18 4166.0 19 3983.0 20 3875.0 21 3923.0 22 4616.0 23 6286.0 24 9445.0 25 13464.0 26 15269.0 27 14492.0 28 16691.0 29 20527.0 30 24523.0 31 29852.0 32 37555.0 33 48599.0 34 65269.0 35 93257.0 36 145341.0 37 178281.0 38 50302.0 39 68.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.480017459663394 17.584502216333224 9.137557046562435 21.797923277440937 2 18.362392015655928 22.25193866467043 36.47499561287571 22.910673706797933 3 18.008312212199094 30.77973081938154 28.00362520852724 23.208331759892133 4 11.306686984442907 16.939205496461977 34.59467924532493 37.15942827377019 5 11.225213998447282 44.586425162463335 32.1063755821302 12.081985256959184 6 29.972522041232487 36.097419026653846 19.704384256040374 14.225674676073297 7 29.224041992185327 35.39403215165523 19.75358690654162 15.628338949617818 8 28.791325228409143 33.994255618320715 19.198363317702512 18.01605583556763 9 29.257427062184295 15.177663057723834 17.472533086912286 38.09237679317959 10-11 24.500808569054907 28.912396874125346 26.813893615414347 19.7729009414054 12-13 25.4922508257863 27.111558342607463 26.67845092041575 20.71773991119049 14-15 23.469830327000793 27.726566183465422 24.116134900946797 24.687468588586988 16-17 23.30837088806884 30.12359048261653 24.54982909471568 22.01820953459895 18-19 22.53575409159645 28.492483213500503 26.815915784002136 22.15584691090091 20-21 24.52251902038096 27.397900816349196 25.909201977008937 22.170378186260898 22-23 24.248813359963133 28.03777903898138 25.614137289485694 22.09927031156979 24-25 23.622110648761847 27.800080746062516 25.879390705983816 22.698417899191817 26-27 23.55270976448179 27.711846993685825 25.78477378367422 22.95066945815817 28-29 23.337213280080324 27.809555331446674 26.18181030614557 22.671421082327434 30-31 23.135587808176197 27.604035063849636 26.497196946768366 22.7631801812058 32-33 22.87088030325833 28.177123566328593 26.04757348355564 22.904422646857434 34-35 23.150663098450448 28.13407800132503 25.928784875768653 22.78647402445587 36-37 23.667601663557726 27.699046880718203 25.679853436321824 22.95349801940225 38-39 23.20980633782285 27.598290232813337 25.98126408816952 23.210639341194295 40-41 22.57606991012444 28.26487522713238 26.247673142623583 22.911381720119596 42-43 23.14916369238185 28.15456988426251 25.95788446021104 22.7383819631446 44-45 22.931595946492276 28.192737095104604 25.968505791042656 22.907161167360464 46-47 23.036524482009874 28.450277945921332 25.57824413703484 22.934953435033957 48-49 23.108769330472995 28.11632464587756 25.786421979220524 22.98848404442892 50-51 22.898899379189665 28.306763272951628 25.93681051540197 22.857526832456735 52-53 23.051326364238143 28.159750143276657 25.736653057235138 23.05227043525006 54-55 22.3397313616677 28.532729571819132 25.873391121618074 23.254147944895102 56-57 22.905644387849453 28.432300832616487 25.701209912317868 22.96084486721619 58-59 22.959465590650836 27.733508374719623 25.75518529563502 23.55184073899452 60-61 22.849257100651684 27.873816789441975 26.30332951077766 22.97359659912868 62-63 22.376297336215902 28.10704006033179 26.087113464751937 23.429549138700366 64-65 22.330586645075233 28.31271373466303 25.76555743999325 23.59114218026849 66-67 22.45909687135909 28.360020836133142 25.77316381576682 23.407718476740943 68-69 22.80206339342586 27.740746355769318 25.989116593723743 23.468073657081074 70-71 22.483334406980333 28.133753462511578 25.859213779407746 23.52369835110034 72-73 22.753386004828066 27.99173220060187 25.674604783306243 23.580277011263824 74-75 22.514703832520418 28.021775722057995 25.802775665969353 23.66074477945223 76-77 22.232829090585987 27.99580167932827 26.310087076280595 23.461282153805143 78-79 22.71185970056422 27.841030254564842 26.225009996001596 23.22210004886934 80-81 22.50841606866579 28.099395686841167 25.94142793834917 23.45076030614387 82-83 22.388285149909233 28.115762390995176 25.86161225241195 23.63434020668365 84-85 22.345425890281785 27.57660538384048 26.28251334742398 23.795455378453752 86-87 22.44655199414457 27.58560177528791 26.21442954548231 23.753416685085206 88-89 22.622771656464273 27.449449414984333 26.40353502846915 23.524243900082244 90-91 22.26812356377053 27.88020582855099 26.190328101728085 23.66134250595039 92-93 22.029521524723446 27.563530143498156 26.526167310853438 23.880781020924964 94-95 21.856455355129775 27.72115851308531 26.442338128817216 23.9800480029677 96-97 22.153934836174134 27.756240132435693 26.493191901253 23.596633130137175 98-99 22.25955907710403 28.15974472475778 25.847840006486283 23.73285619165191 100-101 21.70370740592652 27.980641076902575 26.317473010795684 23.99817850637523 102-103 21.799248966245685 27.807907734581427 26.43140125216719 23.961442047005693 104-105 21.4710227020303 28.058054555955398 26.47552090275001 23.995401839264293 106-107 21.416310466751927 28.419505298155574 26.42441555354772 23.73976868154478 108-109 21.67483006797281 28.72356612910391 25.84910480252344 23.75249900039984 110-111 21.48023341826251 28.76600196810602 25.994049033828766 23.7597155798027 112-113 21.623382176704677 28.675331339318337 25.833471240980003 23.867815242996976 114-115 20.99103636821029 29.04880878270426 25.77317641100426 24.18697843808119 116-117 20.999100359856058 28.837020747256652 26.194188991070238 23.969689901817052 118-119 21.104795097944482 29.120837623298314 25.691142513469657 24.083224765287547 120-121 21.09410619413002 28.919194033945296 25.836484385976505 24.150215385948183 122-123 21.141198442179547 28.705557385069646 25.792835203232485 24.36040896951832 124-125 21.51830126010497 28.80370828828199 25.302281361430936 24.37570909018211 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 142.0 1 208.5 2 328.0 3 396.5 4 370.5 5 335.5 6 318.5 7 291.0 8 311.5 9 319.5 10 312.5 11 316.5 12 320.0 13 327.5 14 339.5 15 386.0 16 471.0 17 551.0 18 656.0 19 801.0 20 975.5 21 1241.5 22 1651.0 23 2234.0 24 3004.5 25 3753.5 26 4775.0 27 5667.5 28 6564.5 29 7987.0 30 10003.5 31 12558.5 32 14619.5 33 15872.5 34 17222.5 35 17798.0 36 18901.0 37 21085.0 38 22284.0 39 23020.0 40 24064.5 41 25944.0 42 27532.5 43 28869.5 44 31060.0 45 33426.5 46 34518.5 47 35187.5 48 35424.0 49 34044.0 50 34429.0 51 33412.0 52 32083.5 53 32578.0 54 31274.0 55 28582.5 56 25141.5 57 22357.0 58 20645.0 59 19729.0 60 18007.5 61 15394.0 62 12908.0 63 10292.5 64 7917.5 65 6182.5 66 4694.5 67 3796.0 68 2942.0 69 2059.5 70 1541.0 71 1226.5 72 1013.5 73 905.5 74 774.5 75 528.5 76 333.5 77 246.0 78 190.5 79 138.0 80 83.5 81 51.5 82 41.5 83 27.5 84 18.0 85 17.0 86 14.5 87 12.5 88 8.5 89 5.5 90 7.5 91 8.0 92 5.5 93 4.0 94 2.5 95 1.0 96 1.0 97 1.0 98 2.5 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.1106643772171639E-4 2 0.0 3 0.0 4 1.1106643772171639E-4 5 5.553321886085818E-4 6 4.4426575088686555E-4 7 4.4426575088686555E-4 8 4.4426575088686555E-4 9 6.663986263302983E-4 10-11 6.663986263302983E-4 12-13 8.885315017737311E-4 14-15 0.0013883304715214546 16-17 0.0010551311583563057 18-19 0.0011661975960780221 20-21 0.0013327972526605966 22-23 0.09646120116131067 24-25 9.440647206345892E-4 26-27 7.774650640520146E-4 28-29 6.663986263302983E-4 30-31 4.997989697477236E-4 32-33 8.329982829128728E-4 34-35 6.108654074694401E-4 36-37 2.776660943042909E-4 38-39 6.108654074694401E-4 40-41 6.663986263302983E-4 42-43 6.108654074694401E-4 44-45 6.663986263302983E-4 46-47 7.774650640520146E-4 48-49 2.776660943042909E-4 50-51 7.219318451911565E-4 52-53 6.663986263302983E-4 54-55 7.219318451911565E-4 56-57 8.329982829128728E-4 58-59 3.887325320260073E-4 60-61 4.997989697477236E-4 62-63 8.329982829128728E-4 64-65 5.553321886085818E-4 66-67 3.3319931316514915E-4 68-69 5.553321886085819E-5 70-71 4.4426575088686555E-4 72-73 1.6659965658257457E-4 74-75 1.6659965658257457E-4 76-77 2.2213287544343277E-4 78-79 2.2213287544343277E-4 80-81 1.1106643772171639E-4 82-83 5.553321886085819E-5 84-85 1.1106643772171639E-4 86-87 1.1106643772171639E-4 88-89 1.6659965658257457E-4 90-91 1.1106643772171639E-4 92-93 2.2213287544343277E-4 94-95 1.1106643772171639E-4 96-97 5.553321886085819E-5 98-99 5.553321886085819E-5 100-101 2.2213287544343277E-4 102-103 3.3319931316514915E-4 104-105 2.2213287544343277E-4 106-107 1.6659965658257457E-4 108-109 2.2213287544343277E-4 110-111 4.4426575088686555E-4 112-113 1.1106643772171639E-4 114-115 1.6659965658257457E-4 116-117 2.2213287544343277E-4 118-119 5.553321886085818E-4 120-121 1.6659965658257457E-4 122-123 1.6659965658257457E-4 124-125 5.553321886085819E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 900362.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.814076321544015 #Duplication Level Percentage of deduplicated Percentage of total 1 60.5645422145886 19.87369510608885 2 11.41092699409168 7.488780585673825 3 7.464887536979322 7.348601681105467 4 5.180411290975231 6.799616459161983 5 3.59722374423306 5.901978724446698 6 2.600461992271531 5.119905497140345 7 1.818079997098065 4.17610310484139 8 1.3071324913985878 3.4313876268098586 9 0.9770276763488748 2.8854234665975502 >10 4.792063823380619 26.371903464596986 >50 0.18912682669634057 4.289589248094209 >100 0.09473212187136422 5.406429484340078 >500 0.002368303046649256 0.4617302604747105 >1k 0.0010149870199925384 0.4448552906280752 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1523 0.16915418465017404 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1299 0.14427530260050958 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 1185 0.1316137287002339 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.6659965658257457E-4 0.0 16-17 0.0 0.0 0.0 2.2213287544343275E-4 0.0 18-19 0.0 0.0 0.0 2.776660943042909E-4 0.0 20-21 0.0 0.0 0.0 3.3319931316514915E-4 0.0 22-23 0.0 0.0 0.0 4.442657508868655E-4 0.0 24-25 0.0 0.0 0.0 7.219318451911565E-4 0.0 26-27 0.0 0.0 0.0 0.001166197596078022 0.0 28-29 0.0 0.0 0.0 0.0018881294412691784 0.0 30-31 0.0 0.0 0.0 0.005497788667224961 0.0 32-33 0.0 0.0 0.0 0.015493768062179433 0.0 34-35 0.0 0.0 0.0 0.02765554299270738 0.0 36-37 0.0 0.0 0.0 0.04481530762071256 0.0 38-39 0.0 0.0 0.0 0.07696904134114946 0.0 40-41 0.0 0.0 0.0 0.11856342226793223 0.0 42-43 0.0 0.0 0.0 0.1598801371004107 0.0 44-45 0.0 0.0 0.0 0.2098600340751831 0.0 46-47 0.0 0.0 0.0 0.2669481830641453 0.0 48-49 0.0 0.0 0.0 0.338752635051235 0.0 50-51 0.0 0.0 0.0 0.40739169356325566 0.0 52-53 0.0 0.0 0.0 0.48408306881010077 0.0 54-55 0.0 0.0 0.0 0.5661056330675883 0.0 56-57 0.0 0.0 0.0 0.6730626125936012 0.0 58-59 0.0 0.0 0.0 0.8083415337386518 0.0 60-61 0.0 0.0 0.0 0.9424542572876242 0.0 62-63 0.0 0.0 0.0 1.075733982553684 0.0 64-65 0.0 0.0 0.0 1.2160664266150727 0.0 66-67 0.0 0.0 0.0 1.3998813810445132 0.0 68-69 0.0 0.0 0.0 1.6265679804345363 0.0 70-71 0.0 0.0 0.0 1.8584747023974801 0.0 72-73 0.0 0.0 0.0 2.0830510394707904 0.0 74-75 0.0 0.0 0.0 2.3231211446062803 0.0 76-77 0.0 0.0 0.0 2.594345385522712 0.0 78-79 0.0 0.0 0.0 2.9360968143924335 0.0 80-81 0.0 0.0 0.0 3.296507404799403 0.0 82-83 0.0 0.0 0.0 3.664026247220562 0.0 84-85 0.0 0.0 0.0 4.040763603972624 0.0 86-87 0.0 0.0 0.0 4.436770987669404 0.0 88-89 0.0 0.0 0.0 4.914523269529367 0.0 90-91 0.0 0.0 0.0 5.408991050266449 0.0 92-93 0.0 0.0 0.0 5.911122415206329 0.0 94-95 0.0 0.0 0.0 6.433190205717256 0.0 96-97 0.0 0.0 0.0 6.976749351927336 0.0 98-99 0.0 0.0 0.0 7.586670694676141 0.0 100-101 0.0 0.0 0.0 8.282279794127252 0.0 102-103 0.0 0.0 0.0 8.948567354019827 0.0 104-105 0.0 0.0 0.0 9.632792143604462 0.0 106-107 0.0 0.0 0.0 10.354835055233341 0.0 108-109 0.0 0.0 0.0 11.118139148475835 0.0 110-111 0.0 0.0 0.0 11.952803427954535 0.0 112-113 0.0 0.0 0.0 12.787856439965259 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGAT 2615 0.0 46.41273 5 TACATGG 11755 0.0 46.31025 2 GTACATG 11970 0.0 46.22399 1 TACCTGG 665 0.0 43.838238 2 ACATGGG 12430 0.0 43.173183 3 ATGGGAG 2550 0.0 41.52967 5 CATGGGA 9335 0.0 40.534187 4 CATGGGG 2935 0.0 38.919903 4 TGGGATT 1210 0.0 31.4683 6 CATGGGT 815 0.0 29.199877 4 ATGGGGG 1430 0.0 29.123306 5 GGTATCA 3960 0.0 28.695702 1 TGGGATA 755 0.0 28.368359 6 CGGGAGT 420 0.0 28.330833 4 GTCGGGA 420 0.0 28.330833 2 AGTACAT 7310 0.0 28.323084 2 ATGGGAC 1445 0.0 27.5858 5 GAACAAA 1555 0.0 27.547411 1 ATGGGAA 4155 0.0 27.205782 5 GAGTACA 8125 0.0 27.166222 1 >>END_MODULE