FastQCFastQC Report
Thu 26 May 2016
SRR936241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936241_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900362
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49960.5548879228576951No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36730.40794702575186426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32760.36385364997634284No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15620.17348577572132096No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13170.14627449847950047No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG9880.10973364046905577No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAT300.00423830659.4927258
GGTATCA53800.055.256771
ATACCGG551.3114279E-554.0842936
ATGGGAT23400.043.502035
TACATGG98200.040.676192
GTACATG99500.040.355621
TATAACG758.163691E-539.6860582
GTATCAA74800.039.6330032
ACATGGG99150.039.3858643
ATGGGAG20650.037.7642175
TATTCCG1951.8189894E-1236.6332865
CATGGGA78050.035.7708444
CATGGGG23550.033.872184
CCGTTGC1606.9849193E-933.4646579
TCAACGC100000.030.717015
ATCAACG100350.030.6691994
CAACGCA101150.030.4080476
AACGCAG101600.030.3904767
TATCAAC102250.030.0993063
CGTTACG400.005358926529.754625108-109
Produced by FastQC (version 0.11.5)