##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936241_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 900362 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.573584846983767 31.0 31.0 34.0 30.0 34.0 2 31.30253831236769 31.0 31.0 34.0 28.0 34.0 3 31.66361196940786 31.0 31.0 34.0 30.0 34.0 4 35.515320504419336 37.0 35.0 37.0 33.0 37.0 5 35.14358669068663 37.0 35.0 37.0 33.0 37.0 6 35.36508537677068 37.0 35.0 37.0 33.0 37.0 7 35.185410979139505 37.0 35.0 37.0 32.0 37.0 8 35.26207347711254 37.0 35.0 37.0 32.0 37.0 9 36.903950855322634 39.0 37.0 39.0 33.0 39.0 10-11 36.78857892714264 39.0 37.0 39.0 32.0 39.0 12-13 36.73195503586335 39.0 37.0 39.0 32.0 39.0 14-15 37.787528793973976 40.0 37.0 41.0 32.0 41.0 16-17 37.7089326293202 40.0 37.0 41.0 32.0 41.0 18-19 37.87586715121252 40.0 37.0 41.0 32.0 41.0 20-21 37.94675808174934 40.0 37.0 41.0 32.0 41.0 22-23 37.845283341589266 40.0 37.0 41.0 32.0 41.0 24-25 37.80764736850289 40.0 37.0 41.0 32.0 41.0 26-27 37.60531763890524 40.0 36.5 41.0 32.0 41.0 28-29 37.41734380171531 39.5 36.0 41.0 31.5 41.0 30-31 37.16963454699332 39.0 36.0 41.0 31.0 41.0 32-33 36.87124456607454 39.0 35.0 41.0 31.0 41.0 34-35 36.612651355788 39.0 35.0 41.0 30.0 41.0 36-37 36.358063756577906 39.0 35.0 41.0 30.0 41.0 38-39 36.30155592972604 39.0 35.0 41.0 30.0 41.0 40-41 36.27055339963259 39.0 35.0 41.0 30.0 41.0 42-43 36.082012568278095 39.0 35.0 41.0 30.0 41.0 44-45 35.76990255030755 39.0 35.0 41.0 28.5 41.0 46-47 35.27371823777547 39.0 35.0 41.0 25.5 41.0 48-49 34.8350835552811 39.0 35.0 41.0 23.5 41.0 50-51 34.57977735621894 39.0 34.0 41.0 23.0 41.0 52-53 34.395785806153526 38.5 34.0 41.0 22.0 41.0 54-55 34.19660369940091 38.0 34.0 40.0 21.5 41.0 56-57 33.96939342175703 38.0 34.0 40.0 20.5 41.0 58-59 33.72627121091294 38.0 33.0 40.0 20.0 41.0 60-61 33.477946092793786 37.0 33.0 40.0 19.0 41.0 62-63 33.25036540858011 37.0 33.0 40.0 18.0 41.0 64-65 32.98648376986145 36.5 33.0 40.0 18.0 41.0 66-67 32.68439361056997 36.0 32.0 39.0 17.5 41.0 68-69 32.38126609075017 35.5 32.0 39.0 14.0 41.0 70-71 32.04052481113152 35.0 32.0 39.0 13.0 40.0 72-73 31.77790099982007 35.0 32.0 37.5 12.5 40.0 74-75 31.606833140447954 35.0 32.0 37.0 12.0 39.5 76-77 31.302529427052676 35.0 32.0 37.0 9.5 39.0 78-79 30.98549583389792 35.0 31.0 36.0 8.0 39.0 80-81 30.731290858565778 35.0 31.0 36.0 7.0 37.5 82-83 30.502760556309575 35.0 31.0 35.5 7.0 37.0 84-85 30.27705245223588 35.0 31.0 35.0 6.0 37.0 86-87 30.094911269022905 35.0 31.0 35.0 2.0 36.0 88-89 29.95382301785282 34.0 31.0 35.0 2.0 36.0 90-91 29.823319953529804 34.0 31.0 35.0 2.0 36.0 92-93 29.70950684280323 34.0 31.0 35.0 2.0 35.5 94-95 29.618658384072184 34.0 31.0 35.0 2.0 35.0 96-97 29.562017832827237 34.0 31.0 35.0 2.0 35.0 98-99 29.476367283381574 34.0 31.0 35.0 2.0 35.0 100-101 29.391902923490775 34.0 31.0 35.0 2.0 35.0 102-103 28.365505763237454 33.5 28.0 34.5 2.0 35.0 104-105 29.11203438172646 34.0 30.0 35.0 2.0 35.0 106-107 29.267805615963358 34.0 30.0 35.0 2.0 35.0 108-109 29.24145510361388 34.0 30.5 35.0 2.0 35.0 110-111 29.20414622118659 34.0 30.5 35.0 2.0 35.0 112-113 29.121810449574724 34.0 30.0 35.0 2.0 35.0 114-115 29.084406605343183 34.0 31.0 35.0 2.0 35.0 116-117 28.975947452246984 34.0 30.0 35.0 2.0 35.0 118-119 28.836725672562814 34.0 30.0 35.0 2.0 35.0 120-121 28.634594196556495 34.0 30.0 35.0 2.0 35.0 122-123 28.368878850951063 34.0 30.0 35.0 2.0 35.0 124-125 26.249235307576285 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18164857464731554 1101 2 0.7106947009563669 1101 3 0.4045986679140583 1101 4 0.28893149861943357 1101 5 0.5581382419548575 1101 6 0.33739529292286363 1101 7 0.5204392803680022 1101 8 0.43984189281487573 1101 9 0.3413543771872227 1101 10-11 0.3423953081930122 1101 12-13 0.5407435685143369 1101 14-15 0.3981317631299959 1101 16-17 0.5404645467018412 1101 18-19 0.4171706272568727 1101 20-21 0.3459652239072213 1101 22-23 0.3925023791307467 1101 24-25 0.4602481219094656 1101 26-27 0.4432153713007807 1101 28-29 0.48681679442039894 1101 30-31 0.3450025325204109 1101 32-33 0.27570590430599395 1101 34-35 0.18234560225702268 1101 36-37 0.44610400829575525 1101 38-39 0.30006187342294766 1101 40-41 0.3134948499887287 1101 42-43 0.26266633045797505 1101 44-45 0.2224809200760305 1101 46-47 0.3605679385113518 1101 48-49 0.07580472128637439 1101 50-51 -0.06950634963817492 1101 52-53 -0.05091473228068111 1101 54-55 -0.1556391522692664 1101 56-57 -0.18780519587904365 1101 58-59 -0.10042675549546232 1101 60-61 0.05243643583458635 1101 62-63 -0.09750725878591027 1101 64-65 0.01723174830028995 1101 66-67 -0.09950252406182614 1101 68-69 -0.1599497575426554 1101 70-71 -0.06481159832122785 1101 72-73 0.05536498586633698 1101 74-75 0.09394748353308202 1101 76-77 0.19127280086975418 1101 78-79 0.13790904642976187 1101 80-81 0.05127652744768696 1101 82-83 0.023740871346486614 1101 84-85 -0.12113457631916802 1101 86-87 -0.054886325598229035 1101 88-89 -0.0664820015965013 1101 90-91 0.05023904103533994 1101 92-93 0.10472179332521847 1101 94-95 0.06287436574172034 1101 96-97 0.09165356562192528 1101 98-99 0.0706599401049246 1101 100-101 0.021031991659477 1101 102-103 0.30217916454945026 1101 104-105 0.08811385461767074 1101 106-107 0.12912667244206943 1101 108-109 0.05675562066069162 1101 110-111 0.029008762693084833 1101 112-113 0.012057631050009121 1101 114-115 0.1137426059004838 1101 116-117 0.18073317920088527 1101 118-119 0.2397014422289665 1101 120-121 0.3044998371923917 1101 122-123 0.43106038096795274 1101 124-125 0.4444835943492542 1102 1 0.17182438572490355 1102 2 0.536690156686987 1102 3 0.20415727781460546 1102 4 0.11121145541162747 1102 5 0.35798086869154844 1102 6 0.001030894666072868 1102 7 0.30707745223077865 1102 8 0.19524752472905504 1102 9 0.37464021918074053 1102 10-11 0.32380850147345086 1102 12-13 0.33223097865326423 1102 14-15 0.20786609098785647 1102 16-17 0.33783483015995586 1102 18-19 0.30705590307912445 1102 20-21 0.37662740217667334 1102 22-23 0.38012669469672744 1102 24-25 0.25058084064899333 1102 26-27 0.33764026048766027 1102 28-29 0.18265105895647338 1102 30-31 0.21793759044121686 1102 32-33 0.19312684519324108 1102 34-35 0.184247583856461 1102 36-37 0.3051618173619488 1102 38-39 0.23106673430125824 1102 40-41 -0.045439574714208675 1102 42-43 0.00992859439807603 1102 44-45 0.2045087071658358 1102 46-47 0.37001499032346885 1102 48-49 0.42269171052166854 1102 50-51 0.31759462998711285 1102 52-53 0.36111221672665295 1102 54-55 0.33502786825443565 1102 56-57 0.3325834881452394 1102 58-59 0.2934184599848493 1102 60-61 0.2863941406060704 1102 62-63 0.2656827624455076 1102 64-65 0.32308063271941023 1102 66-67 0.17778637227668526 1102 68-69 0.18147339960712117 1102 70-71 0.12182471113606397 1102 72-73 0.26164501715474486 1102 74-75 0.22051888815929743 1102 76-77 0.20057086024324278 1102 78-79 0.18182922373076238 1102 80-81 0.15987339115685373 1102 82-83 0.16551648873390334 1102 84-85 0.29580213914252695 1102 86-87 0.18553392619899256 1102 88-89 0.2002843037634996 1102 90-91 0.37443029145438445 1102 92-93 0.3726264940366555 1102 94-95 0.23251855317420933 1102 96-97 0.07166762678332361 1102 98-99 0.19745483338908798 1102 100-101 0.1603585458368535 1102 102-103 0.2052712232524705 1102 104-105 0.1451826146280979 1102 106-107 0.04011300238582649 1102 108-109 0.15585887242066576 1102 110-111 0.21205295757519238 1102 112-113 0.18793156482287188 1102 114-115 0.2929805095768714 1102 116-117 0.3154293277463438 1102 118-119 0.28455515027828326 1102 120-121 0.4151453469327713 1102 122-123 0.4953436342178499 1102 124-125 0.4263065168359397 1103 1 0.09109018943697933 1103 2 0.5243713657365987 1103 3 0.21324797936472706 1103 4 0.13432859350719184 1103 5 0.31118955302823537 1103 6 0.2415030084482339 1103 7 0.23019687327499838 1103 8 0.21119163814594089 1103 9 0.2073183462496644 1103 10-11 0.3286620503171349 1103 12-13 0.31925469848980725 1103 14-15 0.25031295535787734 1103 16-17 0.3836262143117253 1103 18-19 0.37247151388204003 1103 20-21 0.4281124602777169 1103 22-23 0.33795606711414905 1103 24-25 0.2473048093269341 1103 26-27 0.1790529491162829 1103 28-29 0.1650449589176688 1103 30-31 -0.02870761650807907 1103 32-33 0.08415713992191343 1103 34-35 0.17396746605631108 1103 36-37 0.0581536215762668 1103 38-39 0.2060159386936533 1103 40-41 0.10747004334847787 1103 42-43 0.1876333178188503 1103 44-45 0.16639716229447998 1103 46-47 0.2848039317201767 1103 48-49 0.2691314520918837 1103 50-51 0.18367229590040068 1103 52-53 -0.04301763739292852 1103 54-55 0.07926228142248704 1103 56-57 0.11799088033299654 1103 58-59 0.21147430090898922 1103 60-61 0.08365460221828869 1103 62-63 -0.0936990150583199 1103 64-65 -0.10527815549144748 1103 66-67 -0.13026558763473872 1103 68-69 0.02740404362693738 1103 70-71 0.07119575878120088 1103 72-73 0.042593810616381234 1103 74-75 0.034956255462908814 1103 76-77 0.08430713401444123 1103 78-79 -0.005936454419135373 1103 80-81 0.13684528805888974 1103 82-83 0.22854185229347834 1103 84-85 0.15813348631052904 1103 86-87 0.19215904320011035 1103 88-89 0.2382953988939711 1103 90-91 0.20403785258033125 1103 92-93 0.26065699423070754 1103 94-95 0.38325023821861137 1103 96-97 0.3031631864481845 1103 98-99 0.28714503725640483 1103 100-101 0.31524063153874593 1103 102-103 0.28113360710745994 1103 104-105 0.3402879727085981 1103 106-107 0.33481427855412704 1103 108-109 0.35282711660485333 1103 110-111 0.49415920600566565 1103 112-113 0.4276891156755518 1103 114-115 0.5066873333104098 1103 116-117 0.36933202847460933 1103 118-119 0.3893265129703387 1103 120-121 0.3017079111407064 1103 122-123 0.2710599093588222 1103 124-125 0.2798165503952852 1104 1 0.18645285109448295 1104 2 0.4289314022230748 1104 3 0.3063616723578271 1104 4 0.08443076079355905 1104 5 0.35486458633245377 1104 6 0.13610914260818419 1104 7 0.33633438296993745 1104 8 0.32901718847073624 1104 9 0.414615126112011 1104 10-11 0.21033919138529455 1104 12-13 0.23920270010798816 1104 14-15 0.2119276880609462 1104 16-17 0.3332153549469936 1104 18-19 0.31818319341946477 1104 20-21 0.12041989491255123 1104 22-23 0.17530935463324226 1104 24-25 0.1338566054355539 1104 26-27 0.0716427931984498 1104 28-29 0.18966714609638302 1104 30-31 0.2024594611467947 1104 32-33 0.17566218129128686 1104 34-35 -0.008918676039968432 1104 36-37 0.20816199594401041 1104 38-39 0.1614601795765438 1104 40-41 0.16639029102361746 1104 42-43 0.12985662226705585 1104 44-45 0.1590720384182518 1104 46-47 0.20913443322885428 1104 48-49 0.1576639794245267 1104 50-51 0.1815583478111762 1104 52-53 0.2764910320102203 1104 54-55 0.35470013201861406 1104 56-57 0.3307124467778024 1104 58-59 0.3342816981812575 1104 60-61 0.21042655401284804 1104 62-63 0.13758040796062687 1104 64-65 0.15152814409447046 1104 66-67 0.3247184495319999 1104 68-69 0.3032727232339312 1104 70-71 0.1703588373533833 1104 72-73 0.05577068499313498 1104 74-75 0.03324685375350356 1104 76-77 0.13650289323462417 1104 78-79 0.2004198195283351 1104 80-81 0.04449946016889683 1104 82-83 0.20985902438544457 1104 84-85 0.18097463872659958 1104 86-87 0.24010397335788625 1104 88-89 0.25614182071989333 1104 90-91 0.12219992365166021 1104 92-93 0.08952305330478438 1104 94-95 0.17772006343911428 1104 96-97 0.09725268653708241 1104 98-99 0.23621960520344842 1104 100-101 0.2321826216492653 1104 102-103 0.2679845613988725 1104 104-105 0.18243732504788213 1104 106-107 0.1371408067474036 1104 108-109 0.17922157766919966 1104 110-111 0.16519780797583294 1104 112-113 0.221207355048481 1104 114-115 0.09758400907292497 1104 116-117 0.03466704183494329 1104 118-119 0.06007368694331916 1104 120-121 0.16319251905280652 1104 122-123 0.19778522236692098 1104 124-125 -0.0011947640197078613 1105 1 0.023050839173880178 1105 2 0.4554626491957592 1105 3 0.11735840413403764 1105 4 0.06147722463660443 1105 5 0.29305487751538806 1105 6 0.16846098907814167 1105 7 0.25208355061809584 1105 8 0.180618924045433 1105 9 0.14823194344328527 1105 10-11 0.1119610665605606 1105 12-13 0.15964210030480075 1105 14-15 0.10534994155478472 1105 16-17 0.15283234563178638 1105 18-19 0.04183779162168122 1105 20-21 -0.010982331150636071 1105 22-23 0.07849217631874694 1105 24-25 0.12054891822650404 1105 26-27 0.020473676638602 1105 28-29 0.03672192071130809 1105 30-31 -0.10287447382524562 1105 32-33 0.04927191152261656 1105 34-35 0.035921252375231916 1105 36-37 0.18423079689847555 1105 38-39 0.006309540462893892 1105 40-41 -0.21697516369796688 1105 42-43 -0.1788541373782806 1105 44-45 -0.013595974287532897 1105 46-47 0.12670891151122987 1105 48-49 0.02503017854654388 1105 50-51 0.050003417687946694 1105 52-53 -0.004285081913629085 1105 54-55 0.052487325272103647 1105 56-57 -0.09511824801291624 1105 58-59 -0.08983356151761512 1105 60-61 -0.09171716625574078 1105 62-63 -0.09594106493248944 1105 64-65 0.01072123442695272 1105 66-67 0.09318442380428849 1105 68-69 0.26747496561275597 1105 70-71 0.1859940505527291 1105 72-73 0.22664780283784225 1105 74-75 0.1932304871413919 1105 76-77 0.13429569498447336 1105 78-79 0.1648749541067538 1105 80-81 0.1827310502795889 1105 82-83 0.1698214984461508 1105 84-85 0.21902344284221442 1105 86-87 0.2084939779976409 1105 88-89 0.13255681885889103 1105 90-91 0.08303579355493795 1105 92-93 0.1490806959008033 1105 94-95 0.07197135466793725 1105 96-97 0.06066719010867416 1105 98-99 0.06703140955870168 1105 100-101 0.09742243585507282 1105 102-103 0.010451840921231081 1105 104-105 -0.06945426781607367 1105 106-107 0.07629601423627363 1105 108-109 0.12238023183401836 1105 110-111 0.05059175385894221 1105 112-113 0.05033839501666293 1105 114-115 0.27148181207194 1105 116-117 0.3182300685874715 1105 118-119 0.20579109010752816 1105 120-121 0.2532369281467304 1105 122-123 0.3723677717854237 1105 124-125 0.3029195779252163 1106 1 0.1911982452633012 1106 2 0.6075048335729853 1106 3 0.29079679969231975 1106 4 0.09135965263060086 1106 5 0.3053822450288308 1106 6 0.2543069941717704 1106 7 0.3022552486090504 1106 8 0.15408174108863903 1106 9 0.18943447729694896 1106 10-11 0.29254797963502455 1106 12-13 0.19554866071309362 1106 14-15 0.19146358618728954 1106 16-17 0.23524289571327728 1106 18-19 0.2077528746298114 1106 20-21 0.2053454251129736 1106 22-23 0.23935525153193993 1106 24-25 0.1883766022224549 1106 26-27 0.20846453602567294 1106 28-29 0.1409787350532028 1106 30-31 0.10841547897650372 1106 32-33 0.11368307961718926 1106 34-35 0.08033595909313362 1106 36-37 0.017302222265911382 1106 38-39 -0.0702739934754959 1106 40-41 0.17478806751368836 1106 42-43 0.1342754011553282 1106 44-45 0.20878980543066916 1106 46-47 -0.0038159510556212695 1106 48-49 0.1081045942999026 1106 50-51 0.22800364695086728 1106 52-53 0.14562894449147734 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-0.8762982457942528 2216 12-13 -0.3677629309453465 2216 14-15 -0.3589321566761896 2216 16-17 -0.44281865574501467 2216 18-19 -0.670629621642 2216 20-21 -0.5315210827533647 2216 22-23 -0.5314361920024453 2216 24-25 -0.5449115069269084 2216 26-27 -1.0036717347231132 2216 28-29 -1.5284080240745794 2216 30-31 -1.3241636074793561 2216 32-33 -0.9831352724629312 2216 34-35 -1.3515427307056882 2216 36-37 -1.1114280111011965 2216 38-39 -0.8400790684915478 2216 40-41 -0.8070794412541602 2216 42-43 -0.6425521582811058 2216 44-45 -0.8116412432375242 2216 46-47 -0.5629858731915789 2216 48-49 -0.5635352480499094 2216 50-51 -0.3937439505339029 2216 52-53 -0.33377850305964074 2216 54-55 -0.6902409238206744 2216 56-57 -1.2921413224975637 2216 58-59 -1.2731503353479212 2216 60-61 -1.0666685522861528 2216 62-63 -0.8615119656275141 2216 64-65 -0.7588182694503303 2216 66-67 -0.7906743189036121 2216 68-69 -0.6354156139225431 2216 70-71 -0.5891323930810906 2216 72-73 -0.4704514453607196 2216 74-75 -0.4704992333815099 2216 76-77 -0.3287761401396523 2216 78-79 -0.28751150684357185 2216 80-81 -0.43401581169458936 2216 82-83 -0.4718029414878018 2216 84-85 -0.4956844711808479 2216 86-87 -0.4265710062235186 2216 88-89 -0.44178044617358836 2216 90-91 -0.45289974739671024 2216 92-93 -0.3629386645238739 2216 94-95 -0.38078958761509796 2216 96-97 -0.38665726733103867 2216 98-99 -0.48452329121108306 2216 100-101 -0.5600922666179713 2216 102-103 -0.5413824788853567 2216 104-105 -0.31659893636579284 2216 106-107 -0.30257145769537175 2216 108-109 -0.4746459179869049 2216 110-111 -0.6591310400604122 2216 112-113 -0.4201298604160364 2216 114-115 -0.5872881007304471 2216 116-117 -0.5649615627128277 2216 118-119 -0.4830832895025168 2216 120-121 -0.5995277991655676 2216 122-123 -0.6698658212955451 2216 124-125 -0.9619745122247174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35.0 3 42.0 4 104.0 5 236.0 6 625.0 7 1331.0 8 2141.0 9 3795.0 10 6302.0 11 8045.0 12 8630.0 13 11361.0 14 15878.0 15 11242.0 16 4899.0 17 4209.0 18 4029.0 19 3714.0 20 3404.0 21 3520.0 22 3810.0 23 4570.0 24 5626.0 25 7221.0 26 9338.0 27 11840.0 28 14911.0 29 18890.0 30 23706.0 31 29688.0 32 37048.0 33 47162.0 34 63154.0 35 89316.0 36 148832.0 37 216222.0 38 75252.0 39 234.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.74344384270779 17.719767549119084 11.055531044017412 21.481257564155708 2 19.17004125025823 21.679265358890575 36.31522127864694 22.835472112204254 3 18.07987915944733 28.673863787818206 31.350904082811322 21.89535296992314 4 11.984637273271499 16.72162610330745 35.5567377973946 35.73699882602645 5 10.738152102520445 41.929604434758346 34.07253381581039 13.259709646910817 6 29.838775959003154 36.419906659765736 18.327406087773586 15.413911293457522 7 26.608963950055642 35.007696904134114 21.604310266315103 16.779028879495137 8 28.564732851897347 32.21326533105573 20.14734073628163 19.074661080765292 9 28.296618471237124 11.494932038446757 19.362323154464537 40.84612633585158 10-11 24.94385591573167 26.095059542717262 28.24952630164312 20.711558239907948 12-13 26.672105219900438 25.204029046094796 28.68529547004427 19.438570263960493 14-15 23.676045677208542 25.68396138662082 27.601857698268972 23.038135237901667 16-17 21.52162130343129 28.204211195052658 27.956255372838925 22.31791212867713 18-19 20.839506776163365 25.22324353982065 32.48382317334583 21.453426510670155 20-21 22.366114962648357 24.500256563471137 32.462109684771235 20.67151878910927 22-23 21.894915600613977 25.047980701095778 32.341547066624315 20.71555663166593 24-25 21.448261921316092 25.07169338554937 32.361539025414224 21.118505667720317 26-27 21.568269207274408 24.91853276793112 32.33371688276493 21.179481142029537 28-29 21.399959904993715 24.72712349428535 32.641389042068106 21.23152755865283 30-31 21.24884213238675 24.7552651044802 32.74138624242249 21.25450652071056 32-33 21.22746184312532 25.057310281864403 32.28984563986486 21.425382235145417 34-35 21.37720314407221 25.130086709664234 32.00832777074973 21.48438237551382 36-37 22.08034101839038 24.496924570339484 31.520321826109942 21.902412585160192 38-39 21.642961386642263 24.649529855769124 31.621781016968733 22.085727740619884 40-41 21.273832080874136 25.176540102758665 31.522987420615262 22.026640395751933 42-43 21.976888705254353 25.148249714829635 30.7695767927414 22.105284787174607 44-45 21.919033102424077 25.401443881719217 30.350395959486004 22.3291270563707 46-47 22.367153890328577 25.677601522208317 29.168605897557516 22.786638689905594 48-49 22.732255975581175 25.4432743263552 28.546166841980742 23.27830285608288 50-51 22.666386017448755 26.086881666923063 28.14826498247516 23.09846733315302 52-53 22.614131965058565 26.146762241525522 27.825857083115586 23.413248710300323 54-55 22.06448502835715 26.508216131920175 27.83993593260928 23.58736290711339 56-57 22.699171514033388 26.338611844494775 27.671975537505123 23.290241103966714 58-59 22.801545875047136 25.33533481834191 27.908276007226227 23.954843299384727 60-61 22.698041410930514 25.52768836730931 28.59885465745239 23.175415564307787 62-63 22.462399490866645 25.689520838204103 28.569500757202587 23.278578913726662 64-65 22.44225430486084 25.956504620155417 28.419273203653916 23.181967871329825 66-67 22.701767324489307 26.013390437432804 28.447571150582444 22.837271087495445 68-69 22.94445786511394 25.729977980846392 28.317575616216985 23.007988537822683 70-71 22.5246323337491 26.281431146643325 28.038654243225626 23.15528227638195 72-73 22.740926637281518 26.059722894035207 27.814501497487342 23.38484897119593 74-75 22.499723720241615 26.38024646931129 27.545064144107524 23.574965666339573 76-77 22.047439991958758 26.411479427361435 28.209374376984282 23.33170620369553 78-79 22.40157003690742 26.103862783927777 28.24294921703628 23.251617962128524 80-81 22.215169487760452 26.423708294459992 27.946987871518058 23.414134346261495 82-83 22.1462280129859 26.353318280112088 27.690504142227397 23.809949564674614 84-85 22.165621437190993 25.799443667286603 27.911930832150013 24.123004063372395 86-87 22.109692972299367 26.0035308157806 27.55119210847679 24.33558410344324 88-89 22.24054218704098 25.7772086186148 27.941580723571352 24.040668470772868 90-91 22.018215138557228 26.233853501416114 27.704837007830292 24.043094352196366 92-93 21.932213480001245 25.914193941063314 27.83937686870527 24.314215710230176 94-95 21.687414061580224 26.050086965793458 27.742587915419936 24.519911057206386 96-97 22.14060604041195 25.888749128813437 27.664997875831965 24.30564695494265 98-99 22.242128160800494 26.163955948022178 27.05811269118355 24.53580319999378 100-101 21.977207891771638 25.673858825763823 27.435662913149304 24.91327036931523 102-103 22.20181050908281 25.284318565631647 27.434638245268943 25.079232680016595 104-105 22.07919862854325 25.249942661601267 27.55025370506016 25.120605004795323 106-107 21.95711148071738 25.54690775608578 27.563495611465445 24.932485151731395 108-109 22.416942253173065 25.538664023257557 27.01641856939365 25.027975154175724 110-111 21.986771884423366 25.68009285226383 27.152900800239905 25.1802344630729 112-113 22.20392526250429 25.146761364078152 26.99737079168384 25.65194258173372 114-115 21.696144006274277 25.610681383674212 27.015673479725788 25.677501130325727 116-117 21.755230811102038 25.33712140147413 27.367848077361018 25.539799710062823 118-119 21.669394580790783 25.72688682886787 26.81518293871607 25.788535651625278 120-121 21.62571329399514 25.633599444637277 26.902658799028117 25.838028462339462 122-123 21.590452177583277 25.660550254912195 26.673867889949015 26.07512967755551 124-125 21.65714964840162 25.464586362359036 26.43235660328529 26.445907385954058 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 12.0 1 13.0 2 36.0 3 77.5 4 102.5 5 130.5 6 202.0 7 457.0 8 776.0 9 1021.5 10 1125.5 11 1157.0 12 1302.5 13 1459.0 14 1606.0 15 1769.5 16 1939.5 17 2071.0 18 2229.0 19 2395.5 20 2545.5 21 2729.5 22 2949.5 23 3403.0 24 4004.5 25 4504.5 26 5446.0 27 6295.5 28 7098.5 29 8487.5 30 10531.0 31 12756.0 32 14398.0 33 15516.0 34 16878.0 35 17489.0 36 18468.0 37 20159.0 38 21399.5 39 22476.0 40 23252.0 41 25009.0 42 27036.5 43 28730.5 44 30816.0 45 33034.5 46 34162.0 47 34884.0 48 35151.5 49 33617.5 50 34460.0 51 33699.0 52 31630.0 53 31606.5 54 30399.5 55 27961.5 56 24025.0 57 21040.5 58 19495.0 59 18668.5 60 17188.5 61 14594.5 62 12090.5 63 9597.0 64 7260.0 65 5627.0 66 4307.5 67 3398.5 68 2558.5 69 1776.0 70 1331.5 71 1079.5 72 901.5 73 776.5 74 659.0 75 471.0 76 274.0 77 184.5 78 110.5 79 41.5 80 19.5 81 12.0 82 7.0 83 3.0 84 1.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06208613868643946 2 4.4426575088686555E-4 3 2.2213287544343277E-4 4 1.1106643772171639E-4 5 0.06230827156188289 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.553321886085819E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 5.553321886085819E-5 30-31 0.0 32-33 0.0 34-35 1.1106643772171639E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0025545280675994767 44-45 0.008940848236598168 46-47 0.01010704583267619 48-49 0.008107849953685294 50-51 0.007608050983937572 52-53 0.00616418729355526 54-55 0.006663986263302982 56-57 0.004997989697477237 58-59 0.0033875263505123493 60-61 0.0022213287544343274 62-63 9.440647206345892E-4 64-65 0.001499396909243171 66-67 0.0019991958789908944 68-69 0.0010551311583563057 70-71 0.0023323951921560442 72-73 0.0022768619732951856 74-75 7.219318451911565E-4 76-77 3.887325320260073E-4 78-79 1.1106643772171639E-4 80-81 2.2213287544343277E-4 82-83 1.1106643772171639E-4 84-85 1.6659965658257457E-4 86-87 3.887325320260073E-4 88-89 9.440647206345892E-4 90-91 0.0013327972526605966 92-93 6.663986263302983E-4 94-95 8.885315017737311E-4 96-97 0.0010551311583563057 98-99 0.0018325962224083202 100-101 7.219318451911565E-4 102-103 8.329982829128728E-4 104-105 6.108654074694401E-4 106-107 4.997989697477236E-4 108-109 0.0010551311583563057 110-111 7.774650640520146E-4 112-113 0.01010704583267619 114-115 0.01988089235218723 116-117 0.018548095099526637 118-119 0.011273243428754211 120-121 0.00549778866722496 122-123 0.005775454761529252 124-125 0.004997989697477237 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 900362.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.6340190185291 #Duplication Level Percentage of deduplicated Percentage of total 1 47.20565129903072 12.100705631814064 2 14.3160665791091 7.339566459188229 3 10.003622320606203 7.692991344618048 4 7.12675775832665 7.307497756695806 5 5.022465868738543 6.437299279957854 6 3.5126081647730136 5.402535870025921 7 2.5659940217804267 4.6043717689026 8 1.8437987527008735 3.781117783445954 9 1.4499675316429905 3.3451645754147497 >10 6.538278901557343 28.114082865123684 >50 0.2616495263453853 4.634647999617566 >100 0.14613862279956544 6.745371770190443 >500 0.004812948655071282 0.8319976021557245 >1k 0.00218770393412331 1.6626492928493477 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4996 0.5548879228576951 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3673 0.40794702575186426 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3276 0.36385364997634284 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1562 0.17348577572132096 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1317 0.14627449847950047 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 988 0.10973364046905577 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.6659965658257457E-4 0.0 16-17 0.0 0.0 0.0 2.2213287544343275E-4 0.0 18-19 0.0 0.0 0.0 2.776660943042909E-4 0.0 20-21 0.0 0.0 0.0 3.3319931316514915E-4 0.0 22-23 0.0 0.0 0.0 4.442657508868655E-4 0.0 24-25 0.0 0.0 0.0 7.219318451911565E-4 0.0 26-27 0.0 0.0 0.0 0.001166197596078022 0.0 28-29 0.0 0.0 0.0 0.001777063003547462 0.0 30-31 0.0 0.0 0.0 0.005331189010642386 0.0 32-33 0.0 0.0 0.0 0.01560483449990115 0.0 34-35 0.0 0.0 0.0 0.028099808743594246 0.0 36-37 0.0 0.0 0.0 0.04570383912248629 0.0 38-39 0.0 0.0 0.0 0.07841290503153175 0.0 40-41 0.0 0.0 0.0 0.12000728595831454 0.0 42-43 0.0 0.0 0.0 0.16193486619826247 0.0 44-45 0.0 0.0 0.0 0.21174816351645226 0.0 46-47 0.0 0.0 0.0 0.2691695118185796 0.0 48-49 0.0 0.0 0.0 0.34080736414908674 0.0 50-51 0.0 0.0 0.0 0.4096685555365509 0.0 52-53 0.0 0.0 0.0 0.48685972975314373 0.0 54-55 0.0 0.0 0.0 0.5700484916067092 0.0 56-57 0.0 0.0 0.0 0.6782272019476611 0.0 58-59 0.0 0.0 0.0 0.8151165864396764 0.0 60-61 0.0 0.0 0.0 0.951172972648779 0.0 62-63 0.0 0.0 0.0 1.0879512907030728 0.0 64-65 0.0 0.0 0.0 1.2307827296132001 0.0 66-67 0.0 0.0 0.0 1.4166524131404925 0.0 68-69 0.0 0.0 0.0 1.6463933395678625 0.0 70-71 0.0 0.0 0.0 1.8833535844471445 0.0 72-73 0.0 0.0 0.0 2.1128168447802107 0.0 74-75 0.0 0.0 0.0 2.3567187420171 0.0 76-77 0.0 0.0 0.0 2.6322190407858175 0.0 78-79 0.0 0.0 0.0 2.9819117199526413 0.0 80-81 0.0 0.0 0.0 3.348153298340001 0.0 82-83 0.0 0.0 0.0 3.7231691253073764 0.0 84-85 0.0 0.0 0.0 4.10773666591882 0.0 86-87 0.0 0.0 0.0 4.510741235192067 0.0 88-89 0.0 0.0 0.0 4.994268971813559 0.0 90-91 0.0 0.0 0.0 5.494789873406474 0.0 92-93 0.0 0.0 0.0 6.004362689673709 0.0 94-95 0.0 0.0 0.0 6.53503812910807 0.0 96-97 0.0 0.0 0.0 7.088037922524496 0.0 98-99 0.0 0.0 0.0 7.707066713166482 0.0 100-101 0.0 0.0 0.0 8.416503584113945 0.0 102-103 0.0 0.0 0.0 9.098007245974397 0.0 104-105 0.0 0.0 0.0 9.792783347142594 0.0 106-107 0.0 0.0 0.0 10.527210166577444 0.0 108-109 0.0 0.0 0.0 11.304619697410597 0.0 110-111 0.0 0.0 0.0 12.154333479200588 0.0 112-113 0.0 0.0 0.0 13.005657724337544 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGGAT 30 0.004238306 59.492725 8 GGTATCA 5380 0.0 55.25677 1 ATACCGG 55 1.3114279E-5 54.084293 6 ATGGGAT 2340 0.0 43.50203 5 TACATGG 9820 0.0 40.67619 2 GTACATG 9950 0.0 40.35562 1 TATAACG 75 8.163691E-5 39.686058 2 GTATCAA 7480 0.0 39.633003 2 ACATGGG 9915 0.0 39.385864 3 ATGGGAG 2065 0.0 37.764217 5 TATTCCG 195 1.8189894E-12 36.633286 5 CATGGGA 7805 0.0 35.770844 4 CATGGGG 2355 0.0 33.87218 4 CCGTTGC 160 6.9849193E-9 33.464657 9 TCAACGC 10000 0.0 30.71701 5 ATCAACG 10035 0.0 30.669199 4 CAACGCA 10115 0.0 30.408047 6 AACGCAG 10160 0.0 30.390476 7 TATCAAC 10225 0.0 30.099306 3 CGTTACG 40 0.0053589265 29.754625 108-109 >>END_MODULE