FastQCFastQC Report
Thu 26 May 2016
SRR936240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544746
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26400.1037431633648309No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51500.058.105991
GTACATG281400.054.714241
TACATGG281600.053.8102462
ACATGGG287650.050.734323
CATGGGA210300.048.375514
ATGGGAT78350.044.6485145
CATGGGG69550.044.481044
ATGGGAG70700.043.8416635
TACCTGG23400.042.2047122
GTATCAA70550.042.163992
ATGGGGG39600.033.6528245
TCAACGC89250.033.4629485
ATCAACG91400.032.740894
CAACGCA91650.032.651586
GAGTACA163450.032.6488341
AGTACAT157150.032.557632
TATTCCG3700.032.158595
AACGCAG93150.031.9341857
ATGGGAC41150.031.3731825
ATGGGAA80600.030.115765
Produced by FastQC (version 0.11.5)