##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936240_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2544746 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.104387235504053 31.0 30.0 33.0 23.0 34.0 2 29.246905192109548 31.0 30.0 34.0 23.0 34.0 3 29.541786488710464 31.0 30.0 34.0 23.0 34.0 4 32.79298876980256 35.0 35.0 37.0 23.0 37.0 5 33.295474283091515 37.0 35.0 37.0 27.0 37.0 6 33.095936883288154 37.0 35.0 37.0 26.0 37.0 7 33.00418666538822 37.0 35.0 37.0 26.0 37.0 8 32.95469331713263 37.0 35.0 37.0 25.0 37.0 9 34.200519816123105 39.0 35.0 39.0 21.0 39.0 10-11 34.134580229225236 38.5 34.5 39.0 23.0 39.0 12-13 34.13060321147965 39.0 35.0 39.0 22.5 39.0 14-15 35.24054188512331 39.0 35.0 41.0 23.5 41.0 16-17 35.31414608766455 39.0 35.0 41.0 25.0 41.0 18-19 35.37784321107097 39.5 35.0 41.0 25.0 41.0 20-21 35.506822684857354 40.0 35.0 41.0 25.0 41.0 22-23 35.45199717378473 39.0 35.0 41.0 25.5 41.0 24-25 35.46738927971593 39.0 34.5 41.0 26.5 41.0 26-27 35.57472847977755 39.0 35.0 41.0 27.0 41.0 28-29 35.47509279904556 39.0 34.0 41.0 27.0 41.0 30-31 35.400082562267514 39.0 34.0 41.0 27.0 41.0 32-33 35.23304545915388 39.0 34.0 41.0 26.5 41.0 34-35 35.18812977798177 39.0 34.0 41.0 26.0 41.0 36-37 35.078944421172096 39.0 34.0 41.0 25.5 41.0 38-39 34.82993116012364 39.0 34.0 40.5 25.0 41.0 40-41 34.92400145240428 39.0 33.5 40.5 25.5 41.0 42-43 34.845773212729284 39.0 34.0 41.0 24.5 41.0 44-45 34.737795638543105 39.0 33.0 41.0 24.0 41.0 46-47 34.705013781336135 39.0 33.0 41.0 24.0 41.0 48-49 34.605168452961514 39.0 33.0 40.5 24.0 41.0 50-51 34.47571918769103 38.5 33.0 40.0 24.0 41.0 52-53 34.315886929383126 38.0 33.0 40.0 24.0 41.0 54-55 34.13269300747501 38.0 33.0 40.0 23.0 41.0 56-57 33.92185565867871 38.0 33.0 40.0 23.0 41.0 58-59 33.674680891531025 38.0 32.0 40.0 22.0 41.0 60-61 33.409461494388836 37.0 32.0 40.0 21.5 41.0 62-63 33.15342041995547 37.0 32.0 40.0 20.0 41.0 64-65 32.854364443445434 36.5 31.0 40.0 19.0 41.0 66-67 32.5557690237061 36.0 31.0 39.0 18.0 41.0 68-69 32.256456243570085 35.5 31.0 39.0 17.0 41.0 70-71 31.91184955197886 35.0 31.0 39.0 13.0 40.0 72-73 31.589167445395336 35.0 31.0 38.0 12.0 40.0 74-75 31.24909774885195 35.0 30.0 37.0 9.0 39.5 76-77 30.922382233826088 35.0 30.0 37.0 7.0 39.0 78-79 30.58799581569241 35.0 30.0 36.0 6.5 39.0 80-81 30.269420995258464 34.0 30.0 36.0 2.0 38.0 82-83 29.967273354590205 34.0 30.0 35.5 2.0 37.0 84-85 29.989261010725627 34.0 30.0 35.0 2.0 37.0 86-87 29.8524756498291 34.0 30.0 35.0 2.0 36.5 88-89 29.668016179217886 34.0 30.0 35.0 2.0 36.0 90-91 29.490563105315815 34.0 30.0 35.0 2.0 36.0 92-93 29.35011765417845 34.0 30.0 35.0 2.0 36.0 94-95 29.220282692260838 34.0 30.0 35.0 2.0 35.0 96-97 29.10227916656515 34.0 30.0 35.0 2.0 35.0 98-99 28.97795732855067 34.0 29.0 35.0 2.0 35.0 100-101 28.852918916072568 34.0 29.0 35.0 2.0 35.0 102-103 27.87752451521684 32.5 27.5 34.5 2.0 35.0 104-105 28.513315081348 34.0 29.0 35.0 2.0 35.0 106-107 28.61151584480337 34.0 29.0 35.0 2.0 35.0 108-109 28.54446927119642 34.0 29.0 35.0 2.0 35.0 110-111 28.408780876362513 34.0 29.0 35.0 2.0 35.0 112-113 28.335252712844426 34.0 29.0 35.0 2.0 35.0 114-115 28.19750713823698 34.0 29.0 35.0 2.0 35.0 116-117 28.05471214022932 34.0 29.0 35.0 2.0 35.0 118-119 27.836653638516378 34.0 28.0 35.0 2.0 35.0 120-121 27.60878885358303 34.0 27.5 35.0 2.0 35.0 122-123 27.281127861091047 34.0 27.0 35.0 2.0 35.0 124-125 25.275246920517805 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5918226854239848 1101 2 0.6608106435793033 1101 3 0.6373526751646601 1101 4 0.6480471267187156 1101 5 0.5750176173709818 1101 6 0.5904858972911455 1101 7 0.6107814540085172 1101 8 0.6025725326240376 1101 9 0.694651309870693 1101 10-11 0.7423746188173368 1101 12-13 0.6919184313557025 1101 14-15 0.8407184996051456 1101 16-17 0.8064876439161779 1101 18-19 0.8492461258251609 1101 20-21 0.6987699195470753 1101 22-23 0.810146242241153 1101 24-25 0.96291740215527 1101 26-27 0.8044316275210974 1101 28-29 0.8983520384741936 1101 30-31 0.8990953152038443 1101 32-33 0.9070492193811646 1101 34-35 0.861563301519034 1101 36-37 0.8548022343559154 1101 38-39 0.9471119371684296 1101 40-41 0.9361183445081878 1101 42-43 1.0377574949057546 1101 44-45 1.0047795835414988 1101 46-47 0.9262173950838246 1101 48-49 0.9377625749180467 1101 50-51 0.9186983938391862 1101 52-53 0.7810873585621678 1101 54-55 0.843403893155525 1101 56-57 0.9006677991837293 1101 58-59 0.9301596574575939 1101 60-61 0.8685056106073148 1101 62-63 0.8342636220960244 1101 64-65 0.8382802430844478 1101 66-67 0.8033738115471323 1101 68-69 0.799583670170243 1101 70-71 0.7894594026663668 1101 72-73 0.8357399320425536 1101 74-75 0.7832278216176967 1101 76-77 0.8268591635308695 1101 78-79 0.8566918832636539 1101 80-81 0.8298744677023571 1101 82-83 0.7726585045948795 1101 84-85 0.6095864295238904 1101 86-87 0.7027290074873953 1101 88-89 0.7311696756410271 1101 90-91 0.6518468570824538 1101 92-93 0.7508800468385033 1101 94-95 0.9028983137080182 1101 96-97 0.892337770852702 1101 98-99 0.8408965302736569 1101 100-101 0.8060240212316394 1101 102-103 0.8959332422462225 1101 104-105 0.9092265683192338 1101 106-107 0.834088974252392 1101 108-109 0.8081885050949893 1101 110-111 0.8720662027330839 1101 112-113 0.9096633568391539 1101 114-115 0.9138805161287564 1101 116-117 0.902817493124477 1101 118-119 1.0168235590360624 1101 120-121 1.0356337550250885 1101 122-123 0.9830640237776294 1101 124-125 0.8238949005018554 1102 1 0.27063557949581707 1102 2 0.3348062581832707 1102 3 0.3690280319446835 1102 4 0.4840210772402287 1102 5 0.392326947288133 1102 6 0.2949261543810948 1102 7 0.3448667064032236 1102 8 0.4806121390561273 1102 9 0.42277238458925126 1102 10-11 0.5377317462999116 1102 12-13 0.4644117614079306 1102 14-15 0.6030667551972542 1102 16-17 0.5500954725465022 1102 18-19 0.5577030732911794 1102 20-21 0.49713617218939277 1102 22-23 0.5911142207008524 1102 24-25 0.7197260375354801 1102 26-27 0.5894399487776241 1102 28-29 0.6206277209040962 1102 30-31 0.5491389465941907 1102 32-33 0.5873369011934528 1102 34-35 0.559589705853611 1102 36-37 0.5922655966514796 1102 38-39 0.6323968731369973 1102 40-41 0.5731409694091951 1102 42-43 0.6978691734036389 1102 44-45 0.6780249489403545 1102 46-47 0.5729874686456427 1102 48-49 0.5184772631620547 1102 50-51 0.4797312678434409 1102 52-53 0.5590815509509568 1102 54-55 0.5972977339744503 1102 56-57 0.5717633758252063 1102 58-59 0.6482379771114779 1102 60-61 0.7093819614029968 1102 62-63 0.7385986852190314 1102 64-65 0.6533765018087152 1102 66-67 0.6230047765707809 1102 68-69 0.5367862278969824 1102 70-71 0.5643990139894157 1102 72-73 0.5832690041120365 1102 74-75 0.5599577607193353 1102 76-77 0.6497254169057882 1102 78-79 0.5435374603760934 1102 80-81 0.6020656188921869 1102 82-83 0.6345008425815521 1102 84-85 0.6066014800685551 1102 86-87 0.6274845520439136 1102 88-89 0.6259106819824538 1102 90-91 0.6019518661558436 1102 92-93 0.6039351965391369 1102 94-95 0.630088117965748 1102 96-97 0.6848198384454598 1102 98-99 0.6904018632665974 1102 100-101 0.7046298610352579 1102 102-103 0.6384715488766233 1102 104-105 0.7211181682282799 1102 106-107 0.6335515410172796 1102 108-109 0.6857128864498776 1102 110-111 0.6443629176769754 1102 112-113 0.6887916095441291 1102 114-115 0.6524407158305401 1102 116-117 0.6995672877007593 1102 118-119 0.7266455442193234 1102 120-121 0.6473344750145422 1102 122-123 0.5852966239161574 1102 124-125 0.602194760310681 1103 1 0.26352872758923596 1103 2 0.3303307561350337 1103 3 0.43939544449168366 1103 4 0.48384813854909225 1103 5 0.34591185289661297 1103 6 0.42233651747257994 1103 7 0.3486640687409306 1103 8 0.4035612477720534 1103 9 0.46029684870966037 1103 10-11 0.556524371561494 1103 12-13 0.4760218611925211 1103 14-15 0.5019256073821907 1103 16-17 0.4025498885892702 1103 18-19 0.4779274177503936 1103 20-21 0.32222408899399113 1103 22-23 0.5100536272467693 1103 24-25 0.5459615383624907 1103 26-27 0.3711718262811985 1103 28-29 0.4395364316918773 1103 30-31 0.47270998135066833 1103 32-33 0.6542842534298856 1103 34-35 0.5568892492817099 1103 36-37 0.45804753464577885 1103 38-39 0.6049118610422966 1103 40-41 0.5579128409276422 1103 42-43 0.5489926491536394 1103 44-45 0.6489349764001275 1103 46-47 0.5449283207252336 1103 48-49 0.6025309680864268 1103 50-51 0.4987275344305928 1103 52-53 0.4820083461061486 1103 54-55 0.48670980156067856 1103 56-57 0.4405450265281061 1103 58-59 0.41330750396635096 1103 60-61 0.4285963301533968 1103 62-63 0.4107707731755781 1103 64-65 0.3782980880367788 1103 66-67 0.4531731714095457 1103 68-69 0.3971056411899241 1103 70-71 0.3397650629681266 1103 72-73 0.37428610778297156 1103 74-75 0.36658748062192004 1103 76-77 0.3959397786423686 1103 78-79 0.3291377666735471 1103 80-81 0.35062115105307967 1103 82-83 0.3465004704367445 1103 84-85 0.36092903873946725 1103 86-87 0.39263405015039154 1103 88-89 0.3723351143756659 1103 90-91 0.32787274921449594 1103 92-93 0.4078551424970023 1103 94-95 0.4655440359277563 1103 96-97 0.4849793477424136 1103 98-99 0.3234815210969124 1103 100-101 0.423851174838461 1103 102-103 0.34776920737613537 1103 104-105 0.27839209335071047 1103 106-107 0.26501028540949534 1103 108-109 0.3436349294011052 1103 110-111 0.292866128147967 1103 112-113 0.33520459979093786 1103 114-115 0.3966881836024001 1103 116-117 0.41939264919075825 1103 118-119 0.37243739919891894 1103 120-121 0.3362942340033328 1103 122-123 0.455606220617625 1103 124-125 0.4623177180010032 1104 1 0.1771934326588145 1104 2 0.2795000420723497 1104 3 0.3303083976690253 1104 4 0.33820159484398715 1104 5 0.3494560279977108 1104 6 0.28328493280023537 1104 7 0.12550044129858406 1104 8 0.2900495918816617 1104 9 0.30601636752543726 1104 10-11 0.37593803572258366 1104 12-13 0.2691894406600568 1104 14-15 0.4260663750923186 1104 16-17 0.36983348461124166 1104 18-19 0.30415621743357235 1104 20-21 0.31136601538459274 1104 22-23 0.4493391103403681 1104 24-25 0.3795250438441897 1104 26-27 0.3109862633311167 1104 28-29 0.2887011889836799 1104 30-31 0.3434231355835564 1104 32-33 0.39253928607421074 1104 34-35 0.5067679550192992 1104 36-37 0.3925823597938205 1104 38-39 0.5444852463149772 1104 40-41 0.36207652929590495 1104 42-43 0.4811392597684616 1104 44-45 0.3863722009415227 1104 46-47 0.23758553680227124 1104 48-49 0.26733749020633724 1104 50-51 0.2324677930828969 1104 52-53 0.28846641220859226 1104 54-55 0.3112573585944176 1104 56-57 0.2633598666682815 1104 58-59 0.28246419129342826 1104 60-61 0.3043149296405332 1104 62-63 0.14646032133371278 1104 64-65 0.2104207226694612 1104 66-67 0.19030637832159414 1104 68-69 0.062283906628927355 1104 70-71 0.24267960674721678 1104 72-73 0.30598678960931025 1104 74-75 0.2034807645182184 1104 76-77 0.1601903925254291 1104 78-79 0.0893056638262415 1104 80-81 0.04533509297109006 1104 82-83 0.023123425474501857 1104 84-85 0.05130921649084286 1104 86-87 0.037822085496127755 1104 88-89 0.08850851226871725 1104 90-91 -0.03037663418254155 1104 92-93 0.0403494388515675 1104 94-95 0.0796301362227041 1104 96-97 0.0143832447166794 1104 98-99 0.07055109305967378 1104 100-101 -0.05375215715667636 1104 102-103 -0.02888932404804123 1104 104-105 0.030532660331690664 1104 106-107 0.07728385313355446 1104 108-109 0.08371658373571478 1104 110-111 -0.02992518479476658 1104 112-113 0.005006979492158337 1104 114-115 -0.04594700122617468 1104 116-117 0.020889250938679993 1104 118-119 0.09065514735509339 1104 120-121 0.040454277695925356 1104 122-123 0.101754043223103 1104 124-125 -8.246152877227075E-4 1105 1 0.3751563134633109 1105 2 0.6179243248289303 1105 3 0.43402771148150876 1105 4 0.46829254426723566 1105 5 0.48179853021598973 1105 6 0.3117380531788214 1105 7 0.2912982821740684 1105 8 0.32588958005084123 1105 9 0.4363439333343706 1105 10-11 0.5077666367282063 1105 12-13 0.42215638830867874 1105 14-15 0.53899154516035 1105 16-17 0.5117755875393684 1105 18-19 0.3924307663835904 1105 20-21 0.33874881633106213 1105 22-23 0.3440503644072166 1105 24-25 0.5299797909604251 1105 26-27 0.48196748190550665 1105 28-29 0.5523472996018413 1105 30-31 0.5908647209133377 1105 32-33 0.5442270263877305 1105 34-35 0.5269709275126502 1105 36-37 0.5466288697021326 1105 38-39 0.6855123093140918 1105 40-41 0.5272033408901109 1105 42-43 0.7514198720133649 1105 44-45 0.747598539302956 1105 46-47 0.6831748594878775 1105 48-49 0.5997539350451433 1105 50-51 0.632619375454972 1105 52-53 0.5807264684049827 1105 54-55 0.6776356490410294 1105 56-57 0.6437410408770958 1105 58-59 0.6351409141564872 1105 60-61 0.6494642937339918 1105 62-63 0.582732873832974 1105 64-65 0.5789784718560966 1105 66-67 0.5960665084606092 1105 68-69 0.5965618569394877 1105 70-71 0.5045048803349061 1105 72-73 0.4781584843741733 1105 74-75 0.6059485994782889 1105 76-77 0.5317391207123521 1105 78-79 0.5495411710726188 1105 80-81 0.5938728776501812 1105 82-83 0.5756115946552178 1105 84-85 0.5355538926221648 1105 86-87 0.48168115086140517 1105 88-89 0.4768588524047743 1105 90-91 0.4876043625639852 1105 92-93 0.429025126517935 1105 94-95 0.46378238407836747 1105 96-97 0.45984871351289414 1105 98-99 0.4505274314211043 1105 100-101 0.5269636074100177 1105 102-103 0.48582525743672633 1105 104-105 0.514026818864668 1105 106-107 0.5367170829472165 1105 108-109 0.5101418277463594 1105 110-111 0.6189952529455489 1105 112-113 0.5308998366350188 1105 114-115 0.47489002923818546 1105 116-117 0.4952496472711232 1105 118-119 0.49000445229446044 1105 120-121 0.4950146888168909 1105 122-123 0.5967943419512878 1105 124-125 0.5597343909234596 1106 1 -0.06446314578535706 1106 2 -0.24077741701430355 1106 3 -0.033980217804856494 1106 4 0.1464151882292768 1106 5 0.09227103769031686 1106 6 0.024079792422291746 1106 7 -0.04795006047509531 1106 8 -0.09079181862327346 1106 9 0.03243551009246204 1106 10-11 0.11544602058366138 1106 12-13 0.03276749580899718 1106 14-15 0.038728643821471564 1106 16-17 0.04354911542186102 1106 18-19 -0.06080870456402465 1106 20-21 -0.09190161263033758 1106 22-23 -0.18119612590865586 1106 24-25 -3.7969251875915013 1106 26-27 -0.8627169714936898 1106 28-29 -0.5137030058706671 1106 30-31 -0.5567987393752318 1106 32-33 -5.350690593526476 1106 34-35 -1.1943651897368 1106 36-37 -0.4349831477163022 1106 38-39 0.010676744514924508 1106 40-41 -0.6162236381817721 1106 42-43 -0.20519831984937298 1106 44-45 -0.24465854505945117 1106 46-47 -0.29787479604313205 1106 48-49 -0.06437579327931076 1106 50-51 0.005435247026532863 1106 52-53 -0.06734011810222995 1106 54-55 -0.25185967138013154 1106 56-57 -0.14085875764103406 1106 58-59 0.053938522371751674 1106 60-61 0.10055294575531093 1106 62-63 0.13233380974874365 1106 64-65 0.1594085706472015 1106 66-67 0.10169565183770146 1106 68-69 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2216 124-125 -1.223677558762077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76629.0 3 12471.0 4 9384.0 5 8327.0 6 9670.0 7 9431.0 8 9250.0 9 10309.0 10 12546.0 11 14827.0 12 15121.0 13 14895.0 14 14847.0 15 14173.0 16 13202.0 17 12455.0 18 11014.0 19 9957.0 20 9428.0 21 9665.0 22 11710.0 23 16722.0 24 25431.0 25 37691.0 26 43693.0 27 38105.0 28 42615.0 29 53138.0 30 61765.0 31 75578.0 32 97447.0 33 127080.0 34 174778.0 35 251950.0 36 413129.0 37 576660.0 38 198935.0 39 718.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.37821653938441 18.052231703224457 9.222521139700826 21.347030617690308 2 18.611145168236963 21.57926298283835 37.74662598673973 22.062965862184956 3 17.585055640130683 30.967452154360398 28.698306235671456 22.74918596983746 4 10.686439748830823 16.841687890192276 36.01037277300344 36.461499587973464 5 10.814528092426489 44.81541227441079 32.888426057825505 11.481633575337218 6 29.408024571526926 37.436722266452996 19.91375129974292 13.241501862277158 7 29.36341329996762 35.34122310911029 20.225697992084026 15.06966559883807 8 28.244549289232502 34.60443884853072 19.358560529788416 17.792451332448362 9 29.286482548258086 14.884526799394665 17.807186143137333 38.021804509209915 10-11 24.208170757442208 29.00103705374563 27.606948377987422 19.183843810824737 12-13 24.832804357765763 27.628695501647837 27.236546883648494 20.301953256937903 14-15 22.697201017811704 28.294595577038322 24.82053700374311 24.187666401406858 16-17 22.560204033513052 30.599093009730105 25.212030589307886 21.628672367448953 18-19 21.753069148183663 29.294960466541966 27.2047565902196 21.747213795054783 20-21 23.621744464695958 28.29664089511158 26.594433218347714 21.487181421844753 22-23 23.366862880103305 28.61685555430345 26.25796766775442 21.75831389783882 24-25 22.58589898689015 28.477796603475593 26.8350618022356 22.101242607398664 26-27 22.460188933342817 28.59633741723507 26.633046555065082 22.31042709435703 28-29 22.434798122538034 28.53888770124085 26.79039907070034 22.23591510552078 30-31 22.35967253732487 28.269464095719858 27.08306521254324 22.28779815441203 32-33 22.343707693982363 28.40773559822109 26.825605974449672 22.422950733346877 34-35 22.542429462375775 28.469261984651773 26.874412878255733 22.113895674716723 36-37 22.692195771692038 28.050604199778405 26.67157747021835 22.585622558311208 38-39 22.477866925293576 28.24792973375077 26.97246574378881 22.30173759716684 40-41 22.273066210819493 28.322963792569887 27.10437819596516 22.299591800645455 42-43 22.22812335074592 28.250123491429875 27.04210812151164 22.479645036312572 44-45 22.27197901844257 28.33412124205853 27.006720998966088 22.387178740532814 46-47 22.508081483676307 28.31896887106015 26.545031135227408 22.627918510036128 48-49 22.354776798240135 28.19001406038657 26.88270855388649 22.572500587486807 50-51 22.333568592437967 28.308311510300577 26.93485549641767 22.42326440084379 52-53 22.43226106504286 28.21843079056609 26.942334788440903 22.406973355950146 54-55 22.124084205090902 28.460509632609227 26.929532987086375 22.4858731752135 56-57 22.376325711468674 28.644150620196303 26.71900644413125 22.260517224203777 58-59 22.666114151386044 28.023979791930447 26.587695492559902 22.722210564123603 60-61 22.446090859058643 28.285276011539967 27.01898812895635 22.24964500044504 62-63 22.013663612624594 28.249627168464837 27.061065777503572 22.675643441406994 64-65 21.996008995871065 28.435983012689398 26.847499708219715 22.72050828321982 66-67 22.162591559014807 28.703617287198664 26.550370499758618 22.583420654027908 68-69 22.313783603125035 28.2671596048478 26.76620135408672 22.652855437940442 70-71 21.947109154737937 28.462708148187737 26.900008900742574 22.690173796331756 72-73 22.192858843843503 28.418480144549036 26.62930073068311 22.759360280924355 74-75 21.883608450064408 28.632594003783495 26.7272701551201 22.756527391032005 76-77 21.8923059420887 28.557436103895235 26.909701652803736 22.640556301212325 78-79 22.220670001387173 28.602065986283083 26.522993892109252 22.654270120220485 80-81 21.833008872440104 28.67525643151701 26.61429446063911 22.87744023540378 82-83 21.96707957034544 28.562895570552925 26.63851581024717 22.83150904885446 84-85 21.915227012609357 28.273977178392663 26.916233992700622 22.894561816297358 86-87 21.773129841486597 28.396406309098683 26.927020959922192 22.90344288949253 88-89 21.923753886950024 28.165449970586305 27.06429318910864 22.846502953355028 90-91 21.821495607505447 28.49119860449315 26.86551093560301 22.821794852398387 92-93 21.67974529391446 28.280663188376636 27.008311664427403 23.031279853281497 94-95 21.526983245613053 28.259055505353892 27.078835077816503 23.135126171216548 96-97 21.89816303000628 28.407625315651853 26.8543391961596 22.83987245818227 98-99 21.629256367518536 28.579144793861357 26.548046424544204 23.243552414075904 100-101 21.411001514305145 28.46980123443279 26.930247847366218 23.188949403895855 102-103 21.560717571929803 28.257779194217992 26.84768327209216 23.333819961760042 104-105 21.421089580998853 28.158089825229037 27.103989990295634 23.31683060347648 106-107 21.145610057485438 28.58148295802524 27.045034024218275 23.227872960271053 108-109 21.294991310464482 29.021869728684113 26.467824157989412 23.215314802861997 110-111 21.265418672287712 28.74697953372528 26.64885333295608 23.338748461030924 112-113 21.42844792422312 28.630516981342645 26.671314172719036 23.269720921715198 114-115 21.055512656524154 28.941756214301297 26.603713156772706 23.399017972401843 116-117 20.904640168316845 28.927158912487382 26.768182567503867 23.400018351691905 118-119 21.1418877391426 28.9940606338782 26.355671909949873 23.508379717029328 120-121 20.91680579425726 28.891100870761555 26.63736161823284 23.554731716748343 122-123 20.967985956843307 28.595424970930083 26.640834164136272 23.795754908090338 124-125 21.081680198197418 28.741710091284535 26.104009630863406 24.072600079654645 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1711.0 1 2067.0 2 2539.5 3 2383.5 4 1845.0 5 1427.0 6 1217.0 7 1182.5 8 1161.5 9 1079.5 10 1043.5 11 1023.5 12 1013.0 13 1045.5 14 1166.0 15 1275.5 16 1358.0 17 1622.0 18 1999.0 19 2420.5 20 3094.0 21 4103.5 22 5577.0 23 7477.0 24 9707.0 25 12865.5 26 17163.5 27 21057.0 28 24238.5 29 27777.0 30 32368.5 31 37633.0 32 43913.5 33 49860.5 34 56241.5 35 61119.5 36 65892.5 37 73289.0 38 75682.0 39 74242.5 40 73318.5 41 75547.0 42 79833.5 43 83066.5 44 87696.0 45 91294.5 46 91569.5 47 91212.5 48 92055.5 49 92836.5 50 92374.5 51 88400.5 52 84487.0 53 83711.0 54 81828.5 55 75633.0 56 68137.5 57 61924.0 58 56487.0 59 50865.0 60 43008.5 61 35892.5 62 29559.0 63 23032.0 64 17553.5 65 13606.0 66 10627.5 67 8214.0 68 6293.5 69 4657.0 70 3605.5 71 2850.5 72 2306.0 73 2142.5 74 1771.5 75 1341.5 76 929.5 77 624.0 78 455.5 79 286.0 80 190.0 81 126.5 82 93.0 83 76.0 84 54.0 85 35.0 86 26.0 87 19.0 88 12.5 89 8.5 90 7.0 91 8.5 92 7.0 93 4.5 94 3.5 95 1.0 96 1.0 97 3.0 98 3.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.1437322231766943E-4 2 7.859330557941736E-5 3 0.0 4 2.7507656952796073E-4 5 8.252297085838822E-4 6 4.7155983347650415E-4 7 7.073397502147562E-4 8 0.0012574928892706777 9 0.0016897560699574733 10-11 0.0014539761532192212 12-13 0.0018665910075111624 14-15 0.0027900623480693163 16-17 0.002593579084120773 18-19 0.002593579084120773 20-21 0.0031437322231766943 22-23 0.09279904556289703 24-25 0.0022202608826185407 26-27 0.0015325694587986383 28-29 0.001336086194850095 30-31 0.0011199546045066973 32-33 0.0016897560699574733 34-35 8.841746877684452E-4 36-37 6.483947710301932E-4 38-39 9.82416319742717E-4 40-41 0.0013164378684552408 42-43 0.0013753828476398037 44-45 0.0013753828476398037 46-47 0.0015718661115883472 48-49 3.1437322231766943E-4 50-51 0.001336086194850095 52-53 0.0012378445628758233 54-55 0.0011985479100861147 56-57 0.0010806579517169887 58-59 7.859330557941736E-4 60-61 9.627679933478626E-4 62-63 0.0012181962364809689 64-65 9.038230141632995E-4 66-67 2.947248959228151E-4 68-69 1.964832639485434E-4 70-71 5.698014654507759E-4 72-73 1.5718661115883472E-4 74-75 3.1437322231766943E-4 76-77 3.7331820150223247E-4 78-79 1.964832639485434E-4 80-81 4.7155983347650415E-4 82-83 1.5718661115883472E-4 84-85 3.3402154871252376E-4 86-87 3.3402154871252376E-4 88-89 5.108564862662128E-4 90-91 2.947248959228151E-4 92-93 3.536698751073781E-4 94-95 3.7331820150223247E-4 96-97 4.7155983347650415E-4 98-99 5.698014654507759E-4 100-101 5.698014654507759E-4 102-103 0.0010020646461375714 104-105 6.090981182404844E-4 106-107 5.698014654507759E-4 108-109 7.269880766096106E-4 110-111 9.038230141632995E-4 112-113 4.7155983347650415E-4 114-115 4.322631806867955E-4 116-117 8.448780349787367E-4 118-119 0.0011788995836912604 120-121 7.662847293993192E-4 122-123 7.466364030044649E-4 124-125 4.126148542919411E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2544746.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.05982019014105 #Duplication Level Percentage of deduplicated Percentage of total 1 53.17538705761506 14.389164123202173 2 12.3141307031373 6.664363252495812 3 8.55180371388604 6.9423081239741 4 6.470119948203437 7.003211296281191 5 4.592802161157569 6.214020032490752 6 3.294686789848517 5.349217926968033 7 2.3027393551893276 4.361819902732919 8 1.664175804501701 3.602583842767948 9 1.2539630068834173 3.0538812142218488 >10 5.9084133141630915 27.25924889513084 >50 0.29986226375799335 5.577505762601482 >100 0.16412693026874536 7.991539135633515 >500 0.006907183306274907 1.214657794015526 >1k 8.817680816520649E-4 0.37647869748377594 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2640 0.1037431633648309 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.964832639485434E-5 0.0 18-19 0.0 0.0 0.0 1.375382847639804E-4 0.0 20-21 0.0 0.0 0.0 1.5718661115883472E-4 0.0 22-23 0.0 0.0 0.0 2.947248959228151E-4 0.0 24-25 0.0 0.0 0.0 4.7155983347650415E-4 0.0 26-27 0.0 0.0 0.0 6.876914238199018E-4 0.0 28-29 0.0 0.0 0.0 9.038230141632997E-4 0.0 30-31 0.0 0.0 0.0 0.002593579084120773 0.0 32-33 0.0 0.0 0.0 0.008841746877684452 0.0 34-35 0.0 0.0 0.0 0.018037163630476284 0.0 36-37 0.0 0.0 0.0 0.032321496919535384 0.0 38-39 0.0 0.0 0.0 0.05874849592061447 0.0 40-41 0.0 0.0 0.0 0.0881227438809217 0.0 42-43 0.0 0.0 0.0 0.12246801841912709 0.0 44-45 0.0 0.0 0.0 0.16268814254939393 0.0 46-47 0.0 0.0 0.0 0.213125396404985 0.0 48-49 0.0 0.0 0.0 0.27258123207581425 0.0 50-51 0.0 0.0 0.0 0.33182093615630004 0.0 52-53 0.0 0.0 0.0 0.39381140593206554 0.0 54-55 0.0 0.0 0.0 0.46745333325997956 0.0 56-57 0.0 0.0 0.0 0.5700372453675141 0.0 58-59 0.0 0.0 0.0 0.6904421895151814 0.0 60-61 0.0 0.0 0.0 0.8069371167102728 0.0 62-63 0.0 0.0 0.0 0.9229604840718877 0.0 64-65 0.0 0.0 0.0 1.0486508280197708 0.0 66-67 0.0 0.0 0.0 1.2136378247573627 0.0 68-69 0.0 0.0 0.0 1.4313412812123487 0.0 70-71 0.0 0.0 0.0 1.6431305914224836 0.0 72-73 0.0 0.0 0.0 1.8387886256624433 0.0 74-75 0.0 0.0 0.0 2.0485345099275136 0.0 76-77 0.0 0.0 0.0 2.293667030029716 0.0 78-79 0.0 0.0 0.0 2.607293615944381 0.0 80-81 0.0 0.0 0.0 2.943299645622785 0.0 82-83 0.0 0.0 0.0 3.281309804593464 0.0 84-85 0.0 0.0 0.0 3.611833951207704 0.0 86-87 0.0 0.0 0.0 3.984601999570881 0.0 88-89 0.0 0.0 0.0 4.433055401207036 0.0 90-91 0.0 0.0 0.0 4.91744559181938 0.0 92-93 0.0 0.0 0.0 5.394467660033653 0.0 94-95 0.0 0.0 0.0 5.875537283485267 0.0 96-97 0.0 0.0 0.0 6.379340020575727 0.0 98-99 0.0 0.0 0.0 6.952737129756762 0.0 100-101 0.0 0.0 0.0 7.6043935229685005 0.0 102-103 0.0 0.0 0.0 8.229996235380662 0.0 104-105 0.0 0.0 0.0 8.865992912455702 0.0 106-107 0.0 0.0 0.0 9.516313219472593 0.0 108-109 0.0 0.0 0.0 10.205871234300005 0.0 110-111 0.0 0.0 0.0 10.962312152175501 0.0 112-113 0.0 0.0 0.0 11.716532809168381 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5150 0.0 58.10599 1 GTACATG 28140 0.0 54.71424 1 TACATGG 28160 0.0 53.810246 2 ACATGGG 28765 0.0 50.73432 3 CATGGGA 21030 0.0 48.37551 4 ATGGGAT 7835 0.0 44.648514 5 CATGGGG 6955 0.0 44.48104 4 ATGGGAG 7070 0.0 43.841663 5 TACCTGG 2340 0.0 42.204712 2 GTATCAA 7055 0.0 42.16399 2 ATGGGGG 3960 0.0 33.652824 5 TCAACGC 8925 0.0 33.462948 5 ATCAACG 9140 0.0 32.74089 4 CAACGCA 9165 0.0 32.65158 6 GAGTACA 16345 0.0 32.648834 1 AGTACAT 15715 0.0 32.55763 2 TATTCCG 370 0.0 32.15859 5 AACGCAG 9315 0.0 31.934185 7 ATGGGAC 4115 0.0 31.373182 5 ATGGGAA 8060 0.0 30.11576 5 >>END_MODULE