FastQCFastQC Report
Thu 26 May 2016
SRR936240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544746
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156640.6155427692979968No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126580.4974170310121324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119700.4703809338928128No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29970.11777206841075691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90900.082.048931
GTATCAA146700.050.7715572
GTACATG246150.047.046671
TACATGG249300.045.9492
ACATGGG251450.045.0115283
CATGGGA185200.042.4673464
TACCTGG19950.039.393482
ATGGGAG61100.039.3671045
ATGGGAT72600.038.54385
ATCAACG211050.036.8426744
TCAACGC210750.036.8386155
CAACGCA214600.036.234596
CATGGGG63250.036.1463134
TATCAAC216950.035.9230583
AACGCAG218850.035.5037357
GACGCGT750.00391311431.7304486
ACGCAGA252400.030.595878
CGCAGAG255700.030.1544729
AGTACAT151900.029.5141642
GAGTACA160100.029.2086031
Produced by FastQC (version 0.11.5)