##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936240_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2544746 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.646835479847496 31.0 31.0 34.0 30.0 34.0 2 31.379747919831683 31.0 31.0 34.0 30.0 34.0 3 31.745328610399625 31.0 31.0 34.0 30.0 34.0 4 35.57886720324936 37.0 35.0 37.0 33.0 37.0 5 35.19888743316622 37.0 35.0 37.0 33.0 37.0 6 35.422389110740326 37.0 35.0 37.0 33.0 37.0 7 35.25098182686995 37.0 35.0 37.0 32.0 37.0 8 35.328499583062516 37.0 35.0 37.0 32.0 37.0 9 36.98707847462969 39.0 37.0 39.0 33.0 39.0 10-11 36.86547007049033 39.0 37.0 39.0 32.0 39.0 12-13 36.82047127689758 39.0 37.0 39.0 32.0 39.0 14-15 37.912137203477286 40.0 37.0 41.0 32.5 41.0 16-17 37.823015342199184 40.0 37.0 41.0 32.0 41.0 18-19 37.98662911740504 40.0 37.0 41.0 33.0 41.0 20-21 38.05166095162346 40.0 37.0 41.0 33.0 41.0 22-23 37.94195275284842 40.0 37.0 41.0 32.5 41.0 24-25 37.90734222590388 40.0 37.0 41.0 32.5 41.0 26-27 37.72434773450867 40.0 37.0 41.0 32.0 41.0 28-29 37.555132417930906 40.0 36.5 41.0 32.0 41.0 30-31 37.31909667998299 39.0 36.0 41.0 32.0 41.0 32-33 37.042799752902646 39.0 35.5 41.0 31.0 41.0 34-35 36.799985931798304 39.0 35.0 41.0 30.5 41.0 36-37 36.5603476732059 39.0 35.0 41.0 30.0 41.0 38-39 36.51265941669621 39.0 35.0 41.0 30.0 41.0 40-41 36.5001253563224 39.0 35.0 41.0 30.0 41.0 42-43 36.34731619580107 39.0 35.0 41.0 30.0 41.0 44-45 36.09266818770911 39.0 35.0 41.0 29.5 41.0 46-47 35.67310666762027 39.0 35.0 41.0 27.5 41.0 48-49 35.28785741288129 39.0 35.0 41.0 25.5 41.0 50-51 35.079829774759446 39.0 35.0 41.0 24.0 41.0 52-53 34.92997277527895 39.0 35.0 41.0 24.0 41.0 54-55 34.74789035919498 39.0 34.0 41.0 24.0 41.0 56-57 34.53659952702549 38.0 34.0 40.0 23.0 41.0 58-59 34.312124274878514 38.0 34.0 40.0 23.0 41.0 60-61 34.084319613823936 38.0 34.0 40.0 22.5 41.0 62-63 33.869049799076215 37.0 33.5 40.0 22.0 41.0 64-65 33.612159327492805 37.0 33.0 40.0 22.0 41.0 66-67 33.323787521426496 36.0 33.0 39.5 22.0 41.0 68-69 33.015740667241445 36.0 33.0 39.0 21.0 41.0 70-71 32.68196845579087 35.5 32.5 39.0 20.0 41.0 72-73 32.410629587393004 35.0 32.0 38.5 20.0 40.0 74-75 32.223883248072696 35.0 32.0 37.5 20.0 40.0 76-77 31.918073748814223 35.0 32.0 37.0 19.5 39.0 78-79 31.591552555736406 35.0 32.0 36.5 18.5 39.0 80-81 31.32089784206361 35.0 32.0 36.0 18.0 38.5 82-83 31.06869565764127 35.0 32.0 36.0 18.0 37.0 84-85 30.828806882887328 35.0 32.0 35.0 16.0 37.0 86-87 30.63000334807482 35.0 32.0 35.0 15.0 36.5 88-89 30.469773604123947 35.0 31.5 35.0 12.5 36.0 90-91 30.333833907195455 35.0 31.0 35.0 10.5 36.0 92-93 30.20048346671927 35.0 31.0 35.0 9.0 36.0 94-95 30.090185621669118 34.0 31.0 35.0 7.0 35.0 96-97 30.02363300698773 34.0 31.0 35.0 7.0 35.0 98-99 29.936995283615733 34.0 31.0 35.0 6.0 35.0 100-101 29.848261673267196 34.0 31.0 35.0 2.0 35.0 102-103 28.832735565749978 33.5 29.0 34.5 2.0 35.0 104-105 29.577772594985902 34.0 30.5 35.0 2.0 35.0 106-107 29.723540974226896 34.0 31.0 35.0 2.0 35.0 108-109 29.687865115025232 34.0 31.0 35.0 2.0 35.0 110-111 29.648390841364915 34.0 31.0 35.0 2.0 35.0 112-113 29.566367527446747 34.0 31.0 35.0 2.0 35.0 114-115 29.53655826554006 34.0 31.0 35.0 2.0 35.0 116-117 29.43276794619188 34.0 31.0 35.0 2.0 35.0 118-119 29.30272923112955 34.0 31.0 35.0 2.0 35.0 120-121 29.103915086220788 34.0 31.0 35.0 2.0 35.0 122-123 28.877933003922593 34.0 31.0 35.0 2.0 35.0 124-125 26.78660954767195 32.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1831574677023795 1101 2 0.653202970052174 1101 3 0.39406805920942034 1101 4 0.24873808899549488 1101 5 0.44192446519916473 1101 6 0.3086952246280319 1101 7 0.4357886629415262 1101 8 0.34308773095172995 1101 9 0.25048853746584854 1101 10-11 0.31011652261199174 1101 12-13 0.3472167504326791 1101 14-15 0.5091018559482237 1101 16-17 0.5026024928047974 1101 18-19 0.4128201313038744 1101 20-21 0.39019920842773104 1101 22-23 0.28791837688601163 1101 24-25 0.20156225198861222 1101 26-27 0.2398898655462034 1101 28-29 0.3217252523200145 1101 30-31 0.24376018802878718 1101 32-33 0.2889339789768499 1101 34-35 0.31817222420058755 1101 36-37 0.2640247284682786 1101 38-39 0.2671432971673724 1101 40-41 0.23052382644722513 1101 42-43 0.1879021989088301 1101 44-45 0.2794275596957263 1101 46-47 0.3837051847428583 1101 48-49 0.36784941102444435 1101 50-51 0.26483985253189957 1101 52-53 0.21783821367236555 1101 54-55 0.1643683698716245 1101 56-57 0.006102629591637765 1101 58-59 0.12413072555590787 1101 60-61 0.25181667281571407 1101 62-63 0.20418210400922732 1101 64-65 0.18090757712498373 1101 66-67 0.1652684909062785 1101 68-69 0.1917566554865502 1101 70-71 0.1292355031325485 1101 72-73 0.23008285716511523 1101 74-75 0.2345457962445252 1101 76-77 0.21759171340026384 1101 78-79 0.2697363858291375 1101 80-81 0.26095450919145335 1101 82-83 0.21926372335848043 1101 84-85 0.15478032552778842 1101 86-87 0.2259148044126782 1101 88-89 0.18372577744582586 1101 90-91 0.24133535917409787 1101 92-93 0.27827065396967754 1101 94-95 0.24249344382335636 1101 96-97 0.2712399533753249 1101 98-99 0.289065798671551 1101 100-101 0.26952658568900034 1101 102-103 0.32264823764142037 1101 104-105 0.2268844583371461 1101 106-107 0.2970042931626402 1101 108-109 0.26221188180472055 1101 110-111 0.3010865931715898 1101 112-113 0.3878693499352508 1101 114-115 0.3724631086890646 1101 116-117 0.392227528152425 1101 118-119 0.3730963277955972 1101 120-121 0.45167809376328805 1101 122-123 0.4972041402083036 1101 124-125 0.5383662952241259 1102 1 0.22558677086089673 1102 2 0.6211870812071076 1102 3 0.30333444274939936 1102 4 0.167529211676829 1102 5 0.3594606141566672 1102 6 0.21578007353615902 1102 7 0.4151608216421536 1102 8 0.3356773368221653 1102 9 0.34853249622244675 1102 10-11 0.3524826131061545 1102 12-13 0.38951404657026956 1102 14-15 0.3986735524707825 1102 16-17 0.4669328631200784 1102 18-19 0.4234336143977373 1102 20-21 0.43300590375127257 1102 22-23 0.3921139884060949 1102 24-25 0.39004434637349306 1102 26-27 0.4272525515569683 1102 28-29 0.3506117305380485 1102 30-31 0.2873323775543071 1102 32-33 0.3657173069645481 1102 34-35 0.32236581933435815 1102 36-37 0.21837444004480489 1102 38-39 0.19277143464528734 1102 40-41 0.20889780415156878 1102 42-43 0.20565689902509376 1102 44-45 0.30324973367972774 1102 46-47 0.2942552179504361 1102 48-49 0.14783556982316526 1102 50-51 0.08320394785018692 1102 52-53 0.14104654803192318 1102 54-55 0.0967113877432979 1102 56-57 0.1634383581221286 1102 58-59 0.32378611187843376 1102 60-61 0.2672143987366198 1102 62-63 0.24224655873965162 1102 64-65 0.21974289843614514 1102 66-67 0.1565841801849146 1102 68-69 0.20556384332465427 1102 70-71 0.24070311770079655 1102 72-73 0.20458725696889246 1102 74-75 0.1910432383294065 1102 76-77 0.26521031581291155 1102 78-79 0.19734595427292945 1102 80-81 0.20963855896870243 1102 82-83 0.12780919646306188 1102 84-85 0.1342261974512553 1102 86-87 0.09837202091626196 1102 88-89 0.11817836100533086 1102 90-91 0.11371826940004226 1102 92-93 0.22000420617046856 1102 94-95 0.20880029135266653 1102 96-97 0.15359413811998834 1102 98-99 0.16827246717506128 1102 100-101 0.26758258694655623 1102 102-103 0.2943346852438786 1102 104-105 0.13337370580014607 1102 106-107 0.12628959724381517 1102 108-109 0.11000335620244073 1102 110-111 0.1085256423135732 1102 112-113 0.030732308716338252 1102 114-115 0.022076600922943612 1102 116-117 0.06429543557810646 1102 118-119 0.03828329948406761 1102 120-121 0.08542171868585058 1102 122-123 0.11262394172193524 1102 124-125 -0.029007485795343513 1103 1 0.12581091040667403 1103 2 0.6196157802006788 1103 3 0.30331615181825455 1103 4 0.2227773817929446 1103 5 0.32704874034428855 1103 6 0.1978492536884957 1103 7 0.2822527272449946 1103 8 0.26558855154215166 1103 9 0.24926651266475375 1103 10-11 0.268522219435809 1103 12-13 0.29670998768887813 1103 14-15 0.3399157583680861 1103 16-17 0.4159774232664972 1103 18-19 0.3407083399203117 1103 20-21 0.22632653015927673 1103 22-23 0.2494161200697178 1103 24-25 0.17393840447511622 1103 26-27 0.09906036805574558 1103 28-29 0.23730267830121932 1103 30-31 0.10504843023933574 1103 32-33 0.11593232854990987 1103 34-35 0.1193932473420034 1103 36-37 -0.012094318013346594 1103 38-39 -0.033977560006754004 1103 40-41 0.06678868375694691 1103 42-43 0.12061648088064914 1103 44-45 0.12567164412484289 1103 46-47 0.11531933619072277 1103 48-49 -0.06545893218422094 1103 50-51 -0.035416110039925286 1103 52-53 0.017240839253524598 1103 54-55 0.07816630891832688 1103 56-57 0.04396536523206862 1103 58-59 0.13746897986342788 1103 60-61 0.06795626102572072 1103 62-63 -0.06992668855937723 1103 64-65 0.005137878717007993 1103 66-67 -0.03527032494707072 1103 68-69 -0.009000984479982321 1103 70-71 0.01337699603694631 1103 72-73 0.0774834732628662 1103 74-75 0.05818974167410573 1103 76-77 0.008869723272010077 1103 78-79 0.011560531374907868 1103 80-81 0.013406263868045443 1103 82-83 0.03469752675272275 1103 84-85 0.07778891052169712 1103 86-87 0.08844857190127087 1103 88-89 0.11822725142656054 1103 90-91 0.20292094020605234 1103 92-93 0.16720547366323757 1103 94-95 0.15485631276158074 1103 96-97 0.13823996724446275 1103 98-99 0.09285184108336253 1103 100-101 0.09813914835051918 1103 102-103 0.18363752296494695 1103 104-105 0.060458318409914114 1103 106-107 0.0806577466540439 1103 108-109 0.014914821907012765 1103 110-111 0.11943676722922447 1103 112-113 0.10788235610085195 1103 114-115 0.07069155798120264 1103 116-117 0.10305415935519946 1103 118-119 0.014564190700749435 1103 120-121 -0.009335000240159985 1103 122-123 0.039950737262195446 1103 124-125 0.10588586226058183 1104 1 0.10679998290743242 1104 2 0.33157624580527667 1104 3 0.214911279485257 1104 4 0.103005315457672 1104 5 0.34994478331447 1104 6 0.16014725977890976 1104 7 0.3354243294553001 1104 8 0.26855501835003537 1104 9 0.2955075192915402 1104 10-11 0.22246691447436717 1104 12-13 0.15777882690863265 1104 14-15 0.1372981740591186 1104 16-17 0.31900783509308894 1104 18-19 0.24198352762110176 1104 20-21 0.25866554701073596 1104 22-23 0.1900030107697006 1104 24-25 0.07446405374417253 1104 26-27 0.09744365520505482 1104 28-29 0.1790526013278395 1104 30-31 0.03423931767979127 1104 32-33 0.12927510540111342 1104 34-35 0.04591801372917104 1104 36-37 0.13764731799995644 1104 38-39 0.08521944994485153 1104 40-41 -0.003959434883221036 1104 42-43 0.11225705003755593 1104 44-45 -0.007722057549202077 1104 46-47 0.05228297048722652 1104 48-49 0.035980007530660885 1104 50-51 0.017660839399177064 1104 52-53 0.002016090600150733 1104 54-55 0.025533860316407697 1104 56-57 -0.09016393534922429 1104 58-59 -0.08765644865795252 1104 60-61 -0.08361913643432928 1104 62-63 -0.1655838535021701 1104 64-65 -0.07100367994213741 1104 66-67 -0.04927288329179902 1104 68-69 -0.0995991899939952 1104 70-71 0.010617514168394848 1104 72-73 -0.09303091015048892 1104 74-75 -0.11724631385116169 1104 76-77 -0.09121327469676288 1104 78-79 -0.08179878795198547 1104 80-81 -0.11666432316408759 1104 82-83 -0.12217407404322955 1104 84-85 -0.12874976003117666 1104 86-87 -0.0568289483919564 1104 88-89 -0.13333132340733655 1104 90-91 -0.11236404079267359 1104 92-93 -0.079755370008467 1104 94-95 -0.018798679519321126 1104 96-97 -0.036177551025158294 1104 98-99 -0.12445201824738916 1104 100-101 -0.21650515850288699 1104 102-103 -0.17610223359247712 1104 104-105 -0.1779584807162351 1104 106-107 -0.18614971600979047 1104 108-109 -0.16669610298112048 1104 110-111 -0.09050287530969214 1104 112-113 -0.134299134604138 1104 114-115 -0.11817920725925646 1104 116-117 -0.17044706297110324 1104 118-119 -0.18337263727817898 1104 120-121 -0.22816187161792456 1104 122-123 -0.19178338582339904 1104 124-125 -0.22457987289225656 1105 1 0.09536475664301491 1105 2 0.48569484976860267 1105 3 0.17855369297238965 1105 4 0.1215057591133899 1105 5 0.2918517634978528 1105 6 0.19374308941511487 1105 7 0.3001093338918608 1105 8 0.15338199261800867 1105 9 0.23825744534892124 1105 10-11 0.06976616025964688 1105 12-13 0.16127446429401715 1105 14-15 0.08205933939481724 1105 16-17 0.2512135434633933 1105 18-19 0.14879734010261814 1105 20-21 0.10387406520062115 1105 22-23 0.20561229796284408 1105 24-25 0.1843797591567693 1105 26-27 0.06389958540322027 1105 28-29 0.1394581766014369 1105 30-31 0.1314905007617142 1105 32-33 0.13569147629729628 1105 34-35 0.12858771204327724 1105 36-37 0.2541864336462183 1105 38-39 0.12433879333438114 1105 40-41 0.0774754216680904 1105 42-43 -0.030189711275660613 1105 44-45 0.042788146532437565 1105 46-47 0.08889750760641846 1105 48-49 -0.023268365731688334 1105 50-51 0.03506323514007903 1105 52-53 -0.013558073848237484 1105 54-55 -0.028991428059811142 1105 56-57 -0.01390506223476251 1105 58-59 -0.04794556430421437 1105 60-61 -0.19544256145650962 1105 62-63 -0.13653734359385794 1105 64-65 -0.0718152002024226 1105 66-67 -0.1332606088167907 1105 68-69 -0.08173650144031086 1105 70-71 -0.0969394216494166 1105 72-73 -0.15574534375002003 1105 74-75 0.014774537967817025 1105 76-77 -0.0411485749038043 1105 78-79 0.004860114739525301 1105 80-81 0.060474467134643106 1105 82-83 0.019244884844667354 1105 84-85 0.036389991521620146 1105 86-87 0.14527841629304916 1105 88-89 0.20169159880503074 1105 90-91 0.15766331797692246 1105 92-93 0.08499987991758928 1105 94-95 0.16366028232628338 1105 96-97 0.10762236024369898 1105 98-99 0.135654089413066 1105 100-101 0.14211100535396426 1105 102-103 0.019607245851474886 1105 104-105 0.054803645873533924 1105 106-107 0.05189006512005179 1105 108-109 0.06821405673494141 1105 110-111 0.0999585266423928 1105 112-113 0.11101844895102175 1105 114-115 0.11715612252482899 1105 116-117 0.122049609610972 1105 118-119 0.05601659667627601 1105 120-121 0.007909187240404236 1105 122-123 0.05717032609614847 1105 124-125 0.1711795178205513 1106 1 0.07045607342450566 1106 2 0.5273944468048697 1106 3 0.26856994151001956 1106 4 0.12493810020269791 1106 5 0.25680919232925703 1106 6 0.13918472466794185 1106 7 0.20996699670220664 1106 8 0.1012794318484751 1106 9 -0.02369920355268107 1106 10-11 0.12448327321948227 1106 12-13 0.13185924916337655 1106 14-15 0.04587092135243864 1106 16-17 0.05772498243388924 1106 18-19 0.13155504190945777 1106 20-21 0.1688896637967403 1106 22-23 0.01813082129574184 1106 24-25 0.027062067748993 1106 26-27 0.0929483960102715 1106 28-29 0.032827310879348204 1106 30-31 0.14466335270503805 1106 32-33 0.10423771963539252 1106 34-35 0.2216594980825377 1106 36-37 0.1947916916729966 1106 38-39 0.2485029685586042 1106 40-41 0.21661246183447957 1106 42-43 0.23054927221581778 1106 44-45 0.1652738478193143 1106 46-47 0.1520066652822294 1106 48-49 0.2348518409497089 1106 50-51 0.2714700335282245 1106 52-53 0.27047790041407893 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48-49 -0.6123607479675997 2216 50-51 -0.41309329162157127 2216 52-53 -0.497766615755566 2216 54-55 -0.8036464787593331 2216 56-57 -1.1684848658543245 2216 58-59 -1.1645320102033239 2216 60-61 -0.7626739823267457 2216 62-63 -0.6525437933435683 2216 64-65 -0.511570205978316 2216 66-67 -0.5346019000502693 2216 68-69 -0.5020074871931044 2216 70-71 -0.38303555821725155 2216 72-73 -0.5665992135998437 2216 74-75 -0.5116806667555771 2216 76-77 -0.40148662521827205 2216 78-79 -0.38017470107359586 2216 80-81 -0.3898488225585943 2216 82-83 -0.4502728279008039 2216 84-85 -0.4728867380381274 2216 86-87 -0.41644504667704396 2216 88-89 -0.4648017910037261 2216 90-91 -0.4869538685203203 2216 92-93 -0.3875774529023275 2216 94-95 -0.4638771005513931 2216 96-97 -0.48832167774885704 2216 98-99 -0.545000672491696 2216 100-101 -0.6783444881641998 2216 102-103 -0.6466992295688812 2216 104-105 -0.4960202214092284 2216 106-107 -0.4840007062828491 2216 108-109 -0.4419314637373972 2216 110-111 -0.584631063910809 2216 112-113 -0.46313522195129764 2216 114-115 -0.4953912077280229 2216 116-117 -0.5034630010936851 2216 118-119 -0.5014294949679332 2216 120-121 -0.5637560354524496 2216 122-123 -0.6054885110236015 2216 124-125 -0.7726579228160837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96.0 3 125.0 4 297.0 5 701.0 6 1725.0 7 3359.0 8 5345.0 9 9215.0 10 15770.0 11 19708.0 12 21232.0 13 26815.0 14 37668.0 15 28071.0 16 12743.0 17 10763.0 18 10547.0 19 10076.0 20 9544.0 21 10019.0 22 11014.0 23 12808.0 24 15587.0 25 19909.0 26 25334.0 27 32148.0 28 40178.0 29 51234.0 30 63640.0 31 79838.0 32 100346.0 33 128316.0 34 172146.0 35 244526.0 36 407160.0 37 647453.0 38 258060.0 39 1230.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.38410497073551 17.65007264512806 10.793276934237973 21.172545449898454 2 18.88062258717604 21.696356714409667 37.35051494536172 22.072505753052578 3 18.028232331607683 29.00478712576756 31.44310896743167 21.523871575193084 4 11.247020821339662 16.50872680759762 36.78722229535769 35.457030075705035 5 10.3005090147199 42.41542899748009 34.50757230179728 12.776489686002723 6 29.43957056600956 36.97305623942674 19.028507767968893 14.558865426594805 7 26.93219676934358 34.66479562203851 22.055207081571208 16.347800527046708 8 28.53439989688558 32.407085029311375 20.44038972848371 18.618125345319335 9 28.60517316855985 11.233576946382861 19.72633025064191 40.43491963441538 10-11 24.629550454151417 26.066550453365483 29.10229547467606 20.20160361780704 12-13 26.060361230551106 25.483034456091097 29.279110763903354 19.177493549454443 14-15 23.134116332239053 25.912330739492273 28.155756998930343 22.79779592933833 16-17 21.24765791765301 28.370397965374906 28.468168114356494 21.913776002615588 18-19 20.390683469132767 25.765562803824587 32.757578311858694 21.086175415183956 20-21 21.948005812760883 24.944945389441617 32.83832649702564 20.268722300771866 22-23 21.590406272374533 25.446331382385512 32.524385537888655 20.438876807351303 24-25 21.053633643593507 25.390451542118548 32.91766643900806 20.63824837527989 26-27 20.956197593001423 25.572552231146055 32.724248313977114 20.747001861875408 28-29 20.98502581102904 25.492608199916262 32.83277247174619 20.68959351730851 30-31 20.95602075806387 25.112447371957753 33.08070825143256 20.850823618545817 32-33 20.879942438262994 25.557973172961074 32.643179319271944 20.91890506950399 34-35 21.22119697053649 25.631803000776898 32.36967927187875 20.777320756807864 36-37 21.60995635713741 25.291286438803716 31.87414382417734 21.22461337988153 38-39 21.3480638146204 25.495727274942176 32.04490742887502 21.111301481562403 40-41 21.180208162229157 25.701582790581064 31.90383244535997 21.21437660182981 42-43 21.231266315895784 25.789519393590005 31.57729790099427 21.401916389519943 44-45 21.423618159277545 25.96442005890755 31.09490663276866 21.517055149046246 46-47 21.87622569729649 26.21559696125323 29.955570315232645 21.952607026217642 48-49 22.034048511770308 26.311894617307452 29.62792734886962 22.026129522052617 50-51 22.032926135291824 26.592721524956925 29.400709911088196 21.973642428663055 52-53 21.93353196494584 26.716449672470056 29.385636470351745 21.964381892232364 54-55 21.77584206353339 26.91095834557346 29.23752268312788 22.075676907765267 56-57 22.030225867751366 26.978491976270615 29.129412245537967 21.861869910440053 58-59 22.289952412025908 26.2933233987862 29.09397755413257 22.322746635055317 60-61 22.138780444050422 26.432586935493283 29.68754328869722 21.741089331759074 62-63 21.808989834784548 26.384820259302888 29.797452162969613 22.00873774294295 64-65 21.739217572550906 26.450428656311008 29.655055063551792 22.15529870758629 66-67 22.020225003458204 26.83145411275009 29.146762333220156 22.00155855057155 68-69 22.058394149110292 26.476623831348757 29.31522175244537 22.149760267095584 70-71 21.79276003927427 26.754545827875027 29.31743368537002 22.135260447480682 72-73 22.08118914989144 26.678593533555368 28.952656036625502 22.287561279927694 74-75 21.73236963951865 26.855777005154785 29.008094202806 22.40375915252056 76-77 21.658887842281043 26.83839811626307 29.19523061198265 22.307483429473237 78-79 21.89915950205551 26.783467004408894 28.841573888742726 22.475799604792865 80-81 21.639337047143595 26.794076084906255 28.81220291613747 22.754383951812684 82-83 21.75678320238358 26.74510814848814 28.75069456942983 22.74741407969845 84-85 21.684055159302222 26.502114067909137 28.804256311601367 23.009574461187267 86-87 21.523234630232764 26.575546539994722 28.851873043019 23.049345786753516 88-89 21.748304620839065 26.424886755313278 28.840013659704383 22.986794964143275 90-91 21.67137482657552 26.673034160668735 28.677635497056524 22.977955515699225 92-93 21.552112363895297 26.45137091057133 28.77509301568888 23.221423709844494 94-95 21.392179477859013 26.437302409110647 28.80100522062392 23.36951289240642 96-97 21.76791057665045 26.56530453732447 28.48603835926726 23.180746526757822 98-99 21.557446979077717 26.56146905945409 28.268701691317215 23.612382270150974 100-101 21.398715497438715 26.399343267757697 28.5650428013607 23.636898433442887 102-103 21.50156579297423 26.002950426314598 28.522307195652797 23.97317658505838 104-105 21.29116112743027 25.96891609112969 28.733929992435332 24.005992789004708 106-107 21.246351775492183 26.04841140379867 28.690380240583384 24.014856580125755 108-109 21.328512628904264 26.495568467704476 28.103683787358758 24.072235116032502 110-111 21.295159411354053 26.246880061334803 28.219610672523498 24.238349854787646 112-113 21.36458807196398 25.95136310446095 28.252257102910534 24.43179172066454 114-115 20.91898870051723 26.23684204837492 28.149934469406578 24.694234781701272 116-117 20.668370631640922 26.214104522254388 28.37398428306519 24.7435405630395 118-119 20.958331171760904 26.237893229326325 27.892301815249226 24.911473783663542 120-121 20.7388597660206 26.21851989107842 28.03938798616512 25.00323235673586 122-123 20.81271766802144 26.097899619034294 27.79831375319993 25.291068959744333 124-125 20.58474751950452 26.069788896301095 27.63641865478007 25.709044929414322 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 165.0 1 176.0 2 707.5 3 1392.5 4 1181.5 5 1114.5 6 1732.0 7 3870.5 8 6110.0 9 6816.0 10 5882.5 11 4400.5 12 4277.0 13 4543.5 14 4891.0 15 5339.0 16 5779.0 17 6247.5 18 6646.5 19 7050.5 20 7585.5 21 8257.5 22 9535.5 23 11371.5 24 13383.0 25 16314.5 26 20400.5 27 23510.5 28 26107.0 29 29327.5 30 33087.5 31 37334.5 32 43112.0 33 48494.0 34 54093.0 35 58727.5 36 63405.5 37 70641.0 38 72826.0 39 71186.5 40 69938.0 41 71533.0 42 75995.0 43 80106.5 44 84723.0 45 88297.0 46 88811.0 47 88632.5 48 90302.0 49 90782.5 50 90288.5 51 86814.0 52 83127.5 53 82219.5 54 79630.5 55 73525.5 56 65455.5 57 59352.0 58 54524.0 59 48713.0 60 41146.5 61 33829.0 62 27659.0 63 21883.5 64 16659.5 65 12793.5 66 9595.5 67 7385.5 68 5654.0 69 4006.0 70 3108.0 71 2490.0 72 2145.5 73 2109.0 74 1683.5 75 1157.0 76 737.0 77 470.5 78 287.5 79 120.5 80 55.5 81 24.0 82 14.0 83 8.0 84 4.0 85 2.0 86 2.0 87 2.5 88 0.5 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06134207500473525 2 3.929665278970868E-4 3 1.5718661115883472E-4 4 3.929665278970868E-5 5 0.07049819510473737 6 3.929665278970868E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.859330557941736E-5 18-19 1.964832639485434E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.964832639485434E-5 30-31 0.0 32-33 0.0 34-35 1.964832639485434E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.002554282431331064 44-45 0.009843811523822023 46-47 0.010845876169959594 48-49 0.009018581815238142 50-51 0.007937923863521153 52-53 0.006660782647855621 54-55 0.007014452522962999 56-57 0.0054032897585849435 58-59 0.0035563470774686357 60-61 0.0022595575354082487 62-63 0.001512921132403784 64-65 0.001964832639485434 66-67 0.0029079523064384424 68-69 0.0015325694587986383 70-71 0.0030454905912024226 72-73 0.0027900623480693163 74-75 9.234713405581539E-4 76-77 6.680430974250475E-4 78-79 1.7683493755368904E-4 80-81 3.929665278970868E-4 82-83 1.5718661115883472E-4 84-85 2.947248959228151E-4 86-87 3.929665278970868E-4 88-89 0.0013753828476398037 90-91 0.0019844809658802883 92-93 5.501531390559215E-4 94-95 0.0012181962364809689 96-97 0.00163081109077291 98-99 0.0016897560699574733 100-101 0.0010020646461375714 102-103 0.0011396029309015518 104-105 8.252297085838822E-4 106-107 5.894497918456302E-4 108-109 0.001100306278111843 110-111 9.627679933478626E-4 112-113 0.010944117801933868 114-115 0.020218127860305114 116-117 0.019019579950219 118-119 0.011867589142492021 120-121 0.006405354404722515 122-123 0.006287464446353389 124-125 0.005639069675323195 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2544746.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.92579905539629 #Duplication Level Percentage of deduplicated Percentage of total 1 42.00587824884799 9.210124456296827 2 14.264469691813458 6.255197921889851 3 10.617544324226069 6.983944299442325 4 8.018139279930441 7.032164425997389 5 5.942052391039375 6.514212335128319 6 4.303352433772303 5.661266443646524 7 3.0052837885704586 4.612527391684516 8 2.1338974748157318 3.7429925790101835 9 1.635934787436254 3.2282259675353773 >10 7.437784846260506 27.729481669894845 >50 0.39665621560308467 5.989976661179055 >100 0.22576421847971526 9.006395416101922 >500 0.010521278820095629 1.5858568647622393 >1k 0.0021768163076059804 0.8450717497392447 >5k 0.0 0.0 >10k+ 5.442040769014951E-4 1.60256181769161 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15664 0.6155427692979968 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12658 0.4974170310121324 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11970 0.4703809338928128 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2997 0.11777206841075691 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.964832639485434E-5 0.0 18-19 0.0 0.0 0.0 1.375382847639804E-4 0.0 20-21 0.0 0.0 0.0 1.5718661115883472E-4 0.0 22-23 0.0 0.0 0.0 3.1437322231766943E-4 0.0 24-25 0.0 0.0 0.0 5.108564862662128E-4 0.0 26-27 0.0 0.0 0.0 7.269880766096106E-4 0.0 28-29 0.0 0.0 0.0 9.038230141632997E-4 0.0 30-31 0.0 0.0 0.0 0.002593579084120773 0.0 32-33 0.0 0.0 0.0 0.008939988509658724 0.0 34-35 0.0 0.0 0.0 0.01807646028326599 0.0 36-37 0.0 0.0 0.0 0.03228220026674568 0.0 38-39 0.0 0.0 0.0 0.05855201265666593 0.0 40-41 0.0 0.0 0.0 0.08780837065860403 0.0 42-43 0.0 0.0 0.0 0.12217329352320427 0.0 44-45 0.0 0.0 0.0 0.16243271430626083 0.0 46-47 0.0 0.0 0.0 0.21294856146743132 0.0 48-49 0.0 0.0 0.0 0.2726794737077885 0.0 50-51 0.0 0.0 0.0 0.33221390268419715 0.0 52-53 0.0 0.0 0.0 0.3943026140919369 0.0 54-55 0.0 0.0 0.0 0.46802313472543033 0.0 56-57 0.0 0.0 0.0 0.5716091114791024 0.0 58-59 0.0 0.0 0.0 0.6931340102312765 0.0 60-61 0.0 0.0 0.0 0.8103952221557672 0.0 62-63 0.0 0.0 0.0 0.9275974891010733 0.0 64-65 0.0 0.0 0.0 1.0550561824244933 0.0 66-67 0.0 0.0 0.0 1.2213989136833303 0.0 68-69 0.0 0.0 0.0 1.4397704132357414 0.0 70-71 0.0 0.0 0.0 1.651638316751456 0.0 72-73 0.0 0.0 0.0 1.8483377122903426 0.0 74-75 0.0 0.0 0.0 2.0589284745903913 0.0 76-77 0.0 0.0 0.0 2.3052202459498905 0.0 78-79 0.0 0.0 0.0 2.6201829180594056 0.0 80-81 0.0 0.0 0.0 2.958684285189956 0.0 82-83 0.0 0.0 0.0 3.298718221779305 0.0 84-85 0.0 0.0 0.0 3.63124649768582 0.0 86-87 0.0 0.0 0.0 4.006509883501143 0.0 88-89 0.0 0.0 0.0 4.457949830749317 0.0 90-91 0.0 0.0 0.0 4.944717468855438 0.0 92-93 0.0 0.0 0.0 5.4241366328898835 0.0 94-95 0.0 0.0 0.0 5.909155569946863 0.0 96-97 0.0 0.0 0.0 6.417005862274664 0.0 98-99 0.0 0.0 0.0 6.996808325860419 0.0 100-101 0.0 0.0 0.0 7.656579477873233 0.0 102-103 0.0 0.0 0.0 8.287290755148057 0.0 104-105 0.0 0.0 0.0 8.928160217169022 0.0 106-107 0.0 3.929665278970868E-5 0.0 9.584826933611449 0.0 108-109 0.0 3.929665278970868E-5 0.0 10.280554522926845 0.0 110-111 0.0 3.929665278970868E-5 0.0 11.04564070441608 0.0 112-113 0.0 3.929665278970868E-5 0.0 11.81058934762055 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9090 0.0 82.04893 1 GTATCAA 14670 0.0 50.771557 2 GTACATG 24615 0.0 47.04667 1 TACATGG 24930 0.0 45.949 2 ACATGGG 25145 0.0 45.011528 3 CATGGGA 18520 0.0 42.467346 4 TACCTGG 1995 0.0 39.39348 2 ATGGGAG 6110 0.0 39.367104 5 ATGGGAT 7260 0.0 38.5438 5 ATCAACG 21105 0.0 36.842674 4 TCAACGC 21075 0.0 36.838615 5 CAACGCA 21460 0.0 36.23459 6 CATGGGG 6325 0.0 36.146313 4 TATCAAC 21695 0.0 35.923058 3 AACGCAG 21885 0.0 35.503735 7 GACGCGT 75 0.003913114 31.730448 6 ACGCAGA 25240 0.0 30.59587 8 CGCAGAG 25570 0.0 30.154472 9 AGTACAT 15190 0.0 29.514164 2 GAGTACA 16010 0.0 29.208603 1 >>END_MODULE