FastQCFastQC Report
Thu 26 May 2016
SRR936239_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936239_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4922401
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63550.057.106811
GTACATG342500.054.6824261
TACATGG352450.052.5141262
ACATGGG351150.051.5225523
CATGGGA226050.046.9793854
CATGGGG125900.043.0493164
GTATCAA87950.041.060712
ATGGGAT99800.037.6756025
ATGGGAG91650.037.1310465
ATCAACG100150.035.464764
TCAACGC100800.035.295095
CAACGCA103400.034.5802046
AACGCAG106000.034.012647
ATGGGGG72950.032.784945
GAGTACA200450.031.757871
AGTACAT195650.031.1078072
TACCTGG41150.030.361492
TATCAAC122200.029.8930873
ATGGGGA70200.029.4927835
ACGCAGA128500.026.9922498
Produced by FastQC (version 0.11.5)