##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936239_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4922401 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.536690326529676 31.0 30.0 33.0 25.0 34.0 2 29.473646905239942 31.0 30.0 34.0 25.0 34.0 3 29.71533282233609 31.0 30.0 34.0 25.0 34.0 4 33.18466211915689 35.0 35.0 37.0 26.0 37.0 5 33.393240818860555 35.0 35.0 37.0 28.0 37.0 6 33.276216220498895 35.0 35.0 37.0 27.0 37.0 7 33.21679135852605 36.0 35.0 37.0 27.0 37.0 8 33.1921895432737 36.0 35.0 37.0 26.0 37.0 9 34.58174455921003 39.0 35.0 39.0 27.0 39.0 10-11 34.41520926474702 38.0 35.0 39.0 26.0 39.0 12-13 34.40339409568623 38.0 35.0 39.0 26.0 39.0 14-15 35.481423293226214 39.0 35.5 41.0 26.0 41.0 16-17 35.482458357212266 39.0 35.0 41.0 26.0 41.0 18-19 35.49056568126002 39.0 35.0 41.0 26.0 41.0 20-21 35.6056177666143 39.0 35.0 41.0 27.0 41.0 22-23 35.46454738246641 39.0 34.5 41.0 26.5 41.0 24-25 35.42937745218238 39.0 34.0 41.0 26.5 41.0 26-27 35.52428489674043 39.0 35.0 41.0 27.0 41.0 28-29 35.40532221978665 39.0 34.5 41.0 27.0 41.0 30-31 35.32780994478101 39.0 34.0 41.0 26.5 41.0 32-33 35.17893280128945 39.0 34.0 41.0 25.5 41.0 34-35 35.14087535737133 39.0 34.0 41.0 25.0 41.0 36-37 35.012421478867736 39.0 34.0 40.0 25.0 41.0 38-39 34.771999172761426 38.0 34.0 40.0 24.5 41.0 40-41 34.865811318500874 38.5 34.0 40.0 24.5 41.0 42-43 34.77254585719449 39.0 34.0 40.5 24.0 41.0 44-45 34.625777339148115 38.0 33.0 40.0 24.0 41.0 46-47 34.59581238911662 38.0 33.0 40.0 24.0 41.0 48-49 34.50400800341134 38.0 33.0 40.0 24.0 41.0 50-51 34.36083133007652 38.0 33.0 40.0 24.0 41.0 52-53 34.2122352486114 38.0 33.0 40.0 23.0 41.0 54-55 34.04692963860522 38.0 33.0 40.0 23.0 41.0 56-57 33.85060857496169 38.0 33.0 40.0 22.0 41.0 58-59 33.62231276972356 38.0 32.5 40.0 22.0 41.0 60-61 33.38868988934465 37.0 32.0 40.0 20.5 41.0 62-63 33.152978495656896 37.0 32.0 40.0 20.0 41.0 64-65 32.8769864543746 36.5 31.5 40.0 19.0 41.0 66-67 32.60235868634027 36.0 31.0 39.0 18.0 41.0 68-69 32.32495341196298 36.0 31.0 39.0 18.0 41.0 70-71 31.995877418357423 35.0 31.0 39.0 14.5 40.0 72-73 31.682892454312437 35.0 31.0 38.0 13.0 40.0 74-75 31.354873160475954 35.0 31.0 37.0 12.5 39.5 76-77 31.033101122805718 35.0 30.0 37.0 9.0 39.0 78-79 30.69231001293881 35.0 30.0 36.5 7.5 39.0 80-81 30.370902837863067 34.0 30.0 36.0 7.0 38.5 82-83 30.059782207910324 34.0 30.0 35.5 6.5 37.0 84-85 30.07439154185122 34.0 30.0 35.0 3.5 37.0 86-87 29.942552425127495 34.0 30.0 35.0 2.0 36.5 88-89 29.759918076564666 34.0 30.0 35.0 2.0 36.0 90-91 29.57863946882832 34.0 30.0 35.0 2.0 36.0 92-93 29.44449202736632 34.0 30.0 35.0 2.0 36.0 94-95 29.307537723968444 34.0 30.0 35.0 2.0 35.0 96-97 29.191014202215545 34.0 30.0 35.0 2.0 35.0 98-99 29.079604343490097 34.0 29.5 35.0 2.0 35.0 100-101 28.965420228867984 34.0 29.0 35.0 2.0 35.0 102-103 27.9654761974898 32.5 28.0 34.5 2.0 35.0 104-105 28.632285240475127 34.0 29.0 35.0 2.0 35.0 106-107 28.75073901943381 34.0 29.0 35.0 2.0 35.0 108-109 28.692165266503075 34.0 29.0 35.0 2.0 35.0 110-111 28.5675335065144 34.0 29.0 35.0 2.0 35.0 112-113 28.509295870043907 34.0 29.0 35.0 2.0 35.0 114-115 28.385848288264203 34.0 29.0 35.0 2.0 35.0 116-117 28.244284547317456 34.0 29.0 35.0 2.0 35.0 118-119 28.026166600404963 34.0 29.0 35.0 2.0 35.0 120-121 27.80308633936975 34.0 29.0 35.0 2.0 35.0 122-123 27.477203401348245 34.0 28.0 35.0 2.0 35.0 124-125 25.46866437740444 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.40104957923493956 1101 2 0.4803510742137931 1101 3 0.39378784747741236 1101 4 0.32036694130515997 1101 5 0.34562554553670566 1101 6 0.4204894135151491 1101 7 0.39041725660085547 1101 8 0.4661885194586617 1101 9 0.4848839057998262 1101 10-11 0.5538840880594975 1101 12-13 0.4637523400636141 1101 14-15 0.5101026286858499 1101 16-17 0.4872005709164853 1101 18-19 0.5099538173769318 1101 20-21 0.38857617885246754 1101 22-23 0.5006026357153246 1101 24-25 0.581964056557922 1101 26-27 0.48930439121156866 1101 28-29 0.6727678205180183 1101 30-31 0.6171680633246481 1101 32-33 0.6795710139834199 1101 34-35 0.5609992866257585 1101 36-37 0.5197013697970263 1101 38-39 0.6584864542135875 1101 40-41 0.5786362575446233 1101 42-43 0.6851820427388375 1101 44-45 0.6417070269806118 1101 46-47 0.5544839593060829 1101 48-49 0.6221344231609223 1101 50-51 0.6020668614314815 1101 52-53 0.48140799238616694 1101 54-55 0.6166067245267328 1101 56-57 0.6070773227418798 1101 58-59 0.61229414083666 1101 60-61 0.6571326918154625 1101 62-63 0.5743109373861301 1101 64-65 0.6274465434807226 1101 66-67 0.6189685563588938 1101 68-69 0.5607184290007297 1101 70-71 0.5370143742841549 1101 72-73 0.4943708979275563 1101 74-75 0.4657373837968528 1101 76-77 0.44237978214762563 1101 78-79 0.4955374685279672 1101 80-81 0.5072236679319708 1101 82-83 0.48652622195620054 1101 84-85 0.28432408803170617 1101 86-87 0.4521064495263225 1101 88-89 0.3799726687483478 1101 90-91 0.34773835815936494 1101 92-93 0.3686761983446054 1101 94-95 0.4433524369527042 1101 96-97 0.5120535006313958 1101 98-99 0.49487647431968185 1101 100-101 0.5275713610809234 1101 102-103 0.5687609719083397 1101 104-105 0.569228218594418 1101 106-107 0.5266759091324822 1101 108-109 0.52023903493075 1101 110-111 0.5285556933364326 1101 112-113 0.46574411402906435 1101 114-115 0.5075444639991638 1101 116-117 0.5190845743644097 1101 118-119 0.5138751459745556 1101 120-121 0.6062736900771846 1101 122-123 0.5892862087819744 1101 124-125 0.34252484439074493 1102 1 0.2281934224650044 1102 2 0.22762131934265994 1102 3 0.25923131778722563 1102 4 0.28376888355217034 1102 5 0.151115810166651 1102 6 0.2249591123654966 1102 7 0.2122042328023639 1102 8 0.20274122064803635 1102 9 0.32370612462016624 1102 10-11 0.41605643817424465 1102 12-13 0.33546455809784703 1102 14-15 0.43681595520622096 1102 16-17 0.3375688451811243 1102 18-19 0.30726794511038946 1102 20-21 0.2254430488047987 1102 22-23 0.4287577699886853 1102 24-25 0.521422353343965 1102 26-27 0.3419257713061228 1102 28-29 0.3314904772851861 1102 30-31 0.3781780545257192 1102 32-33 0.48086743421103506 1102 34-35 0.4063592981002344 1102 36-37 0.37577643413404616 1102 38-39 0.4018894015110135 1102 40-41 0.36154584920798527 1102 42-43 0.49481377881776467 1102 44-45 0.4364072133717798 1102 46-47 0.32044461684006365 1102 48-49 0.3287185894965958 1102 50-51 0.2511489556048758 1102 52-53 0.28365216826550466 1102 54-55 0.28414566462779334 1102 56-57 0.38710803970312213 1102 58-59 0.4702097403914678 1102 60-61 0.42272874705289354 1102 62-63 0.35918404071979637 1102 64-65 0.36986529989373906 1102 66-67 0.2802522384774804 1102 68-69 0.18782328558365435 1102 70-71 0.11395670749060827 1102 72-73 0.13790875126397495 1102 74-75 0.12828566241958583 1102 76-77 0.13676227434167387 1102 78-79 0.19469121779701837 1102 80-81 0.20796306956322042 1102 82-83 0.1984694188509195 1102 84-85 0.1333053409968663 1102 86-87 0.19256255623337282 1102 88-89 0.2790753897284226 1102 90-91 0.2170540394973557 1102 92-93 0.3226658778731597 1102 94-95 0.26773610659004277 1102 96-97 0.23549870659260108 1102 98-99 0.2438888786761204 1102 100-101 0.22594099000872347 1102 102-103 0.23293303619571049 1102 104-105 0.2469035231781369 1102 106-107 0.24760604968093602 1102 108-109 0.2382837584677553 1102 110-111 0.3036342441468207 1102 112-113 0.2429790927488895 1102 114-115 0.22880533164557448 1102 116-117 0.3395881459998513 1102 118-119 0.36388244681252147 1102 120-121 0.2542407988970794 1102 122-123 0.3067395730632789 1102 124-125 0.36190699179906005 1103 1 0.3760283924685055 1103 2 0.3695884299355825 1103 3 0.3778109453489691 1103 4 0.4204510274258766 1103 5 0.37163891513249325 1103 6 0.3656302292423561 1103 7 0.39534639320698517 1103 8 0.5004658933140007 1103 9 0.556344967893942 1103 10-11 0.6413718666643504 1103 12-13 0.5106067061863868 1103 14-15 0.5990647755994587 1103 16-17 0.5768113310039524 1103 18-19 0.6139405577067265 1103 20-21 0.47145879696559945 1103 22-23 0.5628516896768971 1103 24-25 0.6682351571406002 1103 26-27 0.5096326335521368 1103 28-29 0.5798472043280185 1103 30-31 0.6391881509743129 1103 32-33 0.7268579686194272 1103 34-35 0.6604077304001024 1103 36-37 0.6109153389882209 1103 38-39 0.6732934140898408 1103 40-41 0.6994287424467345 1103 42-43 0.7713155537017187 1103 44-45 0.688180010435623 1103 46-47 0.6793635455600651 1103 48-49 0.6228884309749674 1103 50-51 0.532164881664599 1103 52-53 0.525067176366754 1103 54-55 0.57488876214925 1103 56-57 0.5475603545787635 1103 58-59 0.4788912424605769 1103 60-61 0.6100596746452922 1103 62-63 0.5825942742874091 1103 64-65 0.4383680545902777 1103 66-67 0.5397343199497087 1103 68-69 0.576568612315576 1103 70-71 0.5997366300908809 1103 72-73 0.4873196321100046 1103 74-75 0.5182812081040318 1103 76-77 0.5424715300125449 1103 78-79 0.48465385217913237 1103 80-81 0.4838861255384366 1103 82-83 0.5048987888697525 1103 84-85 0.5039745625658796 1103 86-87 0.5352563155388559 1103 88-89 0.4965942949110307 1103 90-91 0.5798861289066224 1103 92-93 0.530265549170668 1103 94-95 0.6293778468794997 1103 96-97 0.6077860900003813 1103 98-99 0.5102578041118342 1103 100-101 0.572271550855632 1103 102-103 0.5301254831544568 1103 104-105 0.6192779060056921 1103 106-107 0.5943222748845649 1103 108-109 0.5970649181498366 1103 110-111 0.5647496772896652 1103 112-113 0.6607353561597336 1103 114-115 0.701208554626831 1103 116-117 0.711437384866116 1103 118-119 0.6839669282781884 1103 120-121 0.6307164021113003 1103 122-123 0.6532123846327309 1103 124-125 0.714291179594646 1104 1 0.24888364393647322 1104 2 0.3263320375336747 1104 3 0.4024465098814929 1104 4 0.4030171229600512 1104 5 0.3186216652064715 1104 6 0.22274633573016445 1104 7 0.1898250261500607 1104 8 0.207015093705877 1104 9 0.41306617080766017 1104 10-11 0.4714086994028648 1104 12-13 0.4318368050050765 1104 14-15 0.4961488784827566 1104 16-17 0.44817572413889195 1104 18-19 0.4055524133422068 1104 20-21 0.4584976047194118 1104 22-23 0.497157079624408 1104 24-25 0.5359214822282823 1104 26-27 0.43850625974292967 1104 28-29 0.5190347725995252 1104 30-31 0.47743745319068864 1104 32-33 0.6693194647062057 1104 34-35 0.537314710033904 1104 36-37 0.5294008210889345 1104 38-39 0.6982918926570534 1104 40-41 0.610042919647988 1104 42-43 0.6900864716872661 1104 44-45 0.6725353846816731 1104 46-47 0.6174537268332116 1104 48-49 0.5212547397773122 1104 50-51 0.548029514832578 1104 52-53 0.6016904851149647 1104 54-55 0.621128809457403 1104 56-57 0.5850271034679793 1104 58-59 0.63757388612688 1104 60-61 0.6609370130634744 1104 62-63 0.5913687305046906 1104 64-65 0.6120694010737253 1104 66-67 0.6360739739357584 1104 68-69 0.6329411361108086 1104 70-71 0.6242327613977707 1104 72-73 0.5746719750856251 1104 74-75 0.5146344177646078 1104 76-77 0.5550858458751655 1104 78-79 0.4680165387021624 1104 80-81 0.4320724455832625 1104 82-83 0.4429623407810048 1104 84-85 0.5343353026840951 1104 86-87 0.5304153376832978 1104 88-89 0.5503255376344818 1104 90-91 0.5406332488017682 1104 92-93 0.5340890452660929 1104 94-95 0.5704704050869083 1104 96-97 0.5165490334139875 1104 98-99 0.5090644227664036 1104 100-101 0.5047961869175879 1104 102-103 0.5592874731734483 1104 104-105 0.5433767032195931 1104 106-107 0.6097574249012645 1104 108-109 0.5757661627470334 1104 110-111 0.6086230389878544 1104 112-113 0.5998202834640622 1104 114-115 0.6327679899560579 1104 116-117 0.6803451649073473 1104 118-119 0.6135668713865527 1104 120-121 0.5855251743050935 1104 122-123 0.6057512545817509 1104 124-125 0.5473372760235051 1105 1 0.09936631346431923 1105 2 0.12390784184437109 1105 3 0.1585655708673066 1105 4 0.14143673733382656 1105 5 0.07069839486891283 1105 6 0.06467345613688735 1105 7 -0.012409152721247096 1105 8 -0.06748197835949554 1105 9 0.06209004402468565 1105 10-11 0.20145225011811618 1105 12-13 0.13103092424260865 1105 14-15 0.10303798848821799 1105 16-17 0.060352496147928036 1105 18-19 0.024534719793827264 1105 20-21 -0.08510783671881228 1105 22-23 -0.08693888469988309 1105 24-25 0.08987798091069976 1105 26-27 -0.05467156677567431 1105 28-29 0.07933321663324477 1105 30-31 0.14483429383098922 1105 32-33 0.30606568776168785 1105 34-35 0.29060810029050543 1105 36-37 0.1890572830816808 1105 38-39 0.30562199583853555 1105 40-41 0.153198612726122 1105 42-43 0.31373075028422903 1105 44-45 0.33865350764796176 1105 46-47 0.13004915247490345 1105 48-49 0.14099827806607124 1105 50-51 0.113318221090843 1105 52-53 0.08641194324839319 1105 54-55 0.08961869251965027 1105 56-57 0.07388962123974352 1105 58-59 0.10611956777853493 1105 60-61 0.17247234090984165 1105 62-63 0.1362271351328701 1105 64-65 0.18479688036088504 1105 66-67 0.22462600858356296 1105 68-69 0.13847686724860608 1105 70-71 0.08027820175822598 1105 72-73 0.10220065321469463 1105 74-75 0.11798784093601355 1105 76-77 0.13605455060793403 1105 78-79 0.14652736919161313 1105 80-81 0.10806319896945027 1105 82-83 0.12567507315448267 1105 84-85 0.11688612363717965 1105 86-87 0.16944776970017372 1105 88-89 0.12034087034957253 1105 90-91 0.1363220654641566 1105 92-93 0.17522231930881915 1105 94-95 0.13192279913795701 1105 96-97 0.17564966270143145 1105 98-99 0.12957625568672526 1105 100-101 0.11760204194232671 1105 102-103 0.1415239788291771 1105 104-105 0.12881191747143106 1105 106-107 0.19034740299202824 1105 108-109 0.21499304363202398 1105 110-111 0.23341064408692702 1105 112-113 0.15075857277113514 1105 114-115 0.14903548222736873 1105 116-117 0.1834139448961558 1105 118-119 0.22669780765270175 1105 120-121 0.22793550714485278 1105 122-123 0.30658610369298245 1105 124-125 0.2718843724458786 1106 1 -0.18931053109980667 1106 2 -0.2876924300377013 1106 3 -0.1712238944525275 1106 4 -0.0796154992606688 1106 5 -0.08282992159980296 1106 6 -0.1868983828390398 1106 7 -0.2492237584364858 1106 8 -0.30030312528282366 1106 9 -0.24075573930716132 1106 10-11 -0.17236780020888176 1106 12-13 -0.24492743583238763 1106 14-15 -0.28829741511685114 1106 16-17 -0.13372498247578335 1106 18-19 -0.2674201223305488 1106 20-21 -0.36201845950375144 1106 22-23 -0.423548835360684 1106 24-25 -4.32991106552646 1106 26-27 -1.2617494359361103 1106 28-29 -0.9591642502219173 1106 30-31 -0.8527067121937506 1106 32-33 -5.613174595819892 1106 34-35 -1.3561848848386902 1106 36-37 -0.582354683115085 1106 38-39 -0.3147749499292445 1106 40-41 -0.8816614953973883 1106 42-43 -0.49915738002798093 1106 44-45 -0.4710752532081415 1106 46-47 -0.5965285100323214 1106 48-49 -0.4007871273446426 1106 50-51 -0.35018136507166275 1106 52-53 -0.2815744107357432 1106 54-55 -0.510605691842315 1106 56-57 -0.2879253163951958 1106 58-59 -0.1354298228036015 1106 60-61 -0.1764575897773497 1106 62-63 -0.23593110938095663 1106 64-65 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-0.7649265154651665 2216 94-95 -0.8128242570576631 2216 96-97 -0.9236868285870372 2216 98-99 -0.782205417107317 2216 100-101 -0.8220294753106394 2216 102-103 -0.7286161310947108 2216 104-105 -0.7499788838827399 2216 106-107 -0.8251831375695637 2216 108-109 -0.8451049328995133 2216 110-111 -0.8237418630138613 2216 112-113 -0.9375656770864396 2216 114-115 -0.9245379817276813 2216 116-117 -0.7780176580171165 2216 118-119 -0.8913980079030459 2216 120-121 -0.8784204411228806 2216 122-123 -0.8120961929047859 2216 124-125 -0.8495287305063286 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 149210.0 3 25456.0 4 16829.0 5 13849.0 6 15022.0 7 16282.0 8 17593.0 9 19709.0 10 22251.0 11 24937.0 12 26212.0 13 26432.0 14 26011.0 15 25357.0 16 23925.0 17 22351.0 18 20119.0 19 19282.0 20 18791.0 21 20091.0 22 23770.0 23 31856.0 24 45347.0 25 62837.0 26 75978.0 27 78090.0 28 90833.0 29 111106.0 30 130217.0 31 158902.0 32 201610.0 33 261357.0 34 353154.0 35 500171.0 36 799923.0 37 1087930.0 38 358462.0 39 1149.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.305184377085496 18.18744368837464 9.52509809777171 22.98227383676815 2 18.322605154604616 20.637799282666265 38.35764907123463 22.681946491494486 3 17.07021312169127 28.53305877885966 30.25382542130372 24.14290267814535 4 10.72150128728144 16.391268548666105 37.85860889905288 35.02862126499958 5 10.86467802868703 42.410089948650686 35.195954626679374 11.529277395982906 6 30.542398520064594 37.77955243980703 19.180611318474796 12.497437721653583 7 27.891669322905845 34.229262999776736 22.090321070751852 15.788746606565574 8 27.00093755935929 34.72665015018218 20.054323591045343 18.218088699413187 9 27.72379489275633 13.873660139827749 19.129979184705167 39.27256578271075 10-11 24.087591240875913 26.9702132613107 29.467642584326992 19.474552913486395 12-13 24.842465404859464 25.77611849181633 28.828543953205916 20.552872150118294 14-15 22.446822191301763 27.314092761788324 25.63991843661925 24.599166610290663 16-17 22.477828013851205 29.697307059787924 26.139519717856334 21.685345208504536 18-19 21.772438786226697 28.75155706572887 27.706470496277213 21.76953365176722 20-21 22.950736078064256 28.145336005252826 27.265047018631925 21.638880898050992 22-23 22.735788918248726 28.326248677028126 27.042226313847056 21.895736090876095 24-25 22.35575882879832 28.213920686731832 27.337719603870443 22.09260088059941 26-27 22.21520772664013 28.33758249241495 27.199405811841977 22.247803969102947 28-29 22.04942193249605 28.413375402678682 27.360697098072485 22.176505566752784 30-31 22.33091443941524 28.11321195524098 27.437438545738225 22.118435059605552 32-33 21.91966750786516 28.399966926312718 27.45020561350674 22.230159952315383 34-35 22.482066803044187 28.416672837480274 27.233329178826725 21.867931180648817 36-37 22.520664305070497 28.04994416315532 27.109209635913707 22.320181895860475 38-39 22.120815477760086 28.195343458816353 27.46743131250297 22.216409750920594 40-41 22.34464218292283 28.19665873313269 27.48582004810909 21.97287903583539 42-43 22.140604735297273 28.054261458749803 27.472781304547095 22.332352501405833 44-45 21.983404688082164 28.27436832052307 27.534905047463067 22.207321943931703 46-47 22.24650717691685 28.222745933923548 27.15821234336856 22.372534545791044 48-49 22.19390488863382 28.14984598429116 27.27102447745124 22.385224649623787 50-51 22.17148092732036 28.102676462790676 27.465958875375705 22.259883734513256 52-53 22.377417731239554 28.00963765837812 27.45314618046332 22.159798429918997 54-55 21.963213565530992 28.089444124137252 27.573146853601465 22.37419545673029 56-57 22.18218611308961 28.355574011013413 27.31816554735308 22.144074328543898 58-59 22.471592484687783 27.829826931175734 27.318823812154168 22.37975677198232 60-61 22.31502539937611 28.020595790844443 27.55240621124195 22.11197259853749 62-63 22.08059976115113 28.071568116875657 27.600909685690368 22.246922436282844 64-65 21.859389815977778 28.139690910928532 27.616588968621297 22.384330304472392 66-67 22.026759180076407 28.4910842517312 27.18598533170553 22.296171236486863 68-69 22.14900920162019 28.112296577640876 27.419219184811293 22.319475035927642 70-71 21.98340700537352 27.852185686249037 27.62359700701602 22.540810301361425 72-73 22.0084507711801 28.18258443914756 27.320604966163803 22.488359823508535 74-75 21.770467731265015 28.48961434068034 27.431398395311547 22.308519532743098 76-77 21.717105948800857 28.248408749657433 27.61397062035147 22.420514681190244 78-79 22.046470912205066 28.203692067533847 27.361845087072524 22.387991933188566 80-81 21.935749609056185 28.251817082479874 27.224306522298907 22.588126786165034 82-83 22.152074564523424 28.30603073361958 27.31398306251155 22.227911639345443 84-85 21.8408083152349 28.16748542602252 27.507612415238015 22.484093843504567 86-87 21.701422500912667 28.1648009390566 27.5848790434397 22.548897516591033 88-89 21.943269765219455 28.090595938878398 27.454268094484295 22.51186620141785 90-91 21.874896518743235 28.178299195776685 27.553693948202795 22.393110337277285 92-93 21.962553740435915 27.971284259924683 27.582814233946433 22.48334776569297 94-95 21.634336830010152 27.937161800329434 27.702153913913328 22.72634745574709 96-97 21.796665275742292 28.04795276112032 27.638019448291946 22.517362514845445 98-99 21.548606253762152 28.082243125836865 27.533321780658586 22.83582883974239 100-101 21.488160047765508 28.026927215399134 27.68124081427675 22.803671922558607 102-103 21.669922888786726 27.85307121303941 27.526704102555456 22.950301795618415 104-105 21.35836364472988 27.850956605632827 27.799893892759364 22.99078585687793 106-107 21.355298191240088 27.95436473996918 27.84641894628978 22.843918122500952 108-109 21.483766887420447 28.078314602883932 27.368555882655883 23.06936262703974 110-111 21.443501097185898 28.227759600541653 27.431201522926322 22.89753777934613 112-113 21.443397299680235 28.10643428585359 27.544104681068916 22.906063733397257 114-115 21.20656681269335 28.210352373685293 27.446952576676196 23.136128236945158 116-117 21.13514168549316 28.171832494694875 27.688700763362966 23.004325056449 118-119 21.206213408644174 28.11867775878393 27.383196395139763 23.291912437432128 120-121 21.125175245831734 28.10045199762472 27.57804115130696 23.19633160523658 122-123 21.29460513307249 27.941266545498834 27.439608148386142 23.324520173042536 124-125 21.377427532095208 27.80193385890501 27.128459653509495 23.692178955490284 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 789.0 1 943.5 2 1257.0 3 1374.0 4 1181.5 5 951.5 6 839.0 7 833.5 8 870.5 9 882.5 10 895.0 11 881.5 12 862.0 13 939.5 14 1057.5 15 1212.5 16 1459.0 17 1706.5 18 2135.0 19 2777.5 20 3803.5 21 5584.5 22 8248.5 23 11922.0 24 16501.5 25 22422.5 26 30769.0 27 39443.0 28 46882.5 29 55263.0 30 67450.5 31 79128.0 32 89978.0 33 102005.5 34 115105.5 35 125134.5 36 136077.0 37 149082.0 38 154261.0 39 155966.5 40 158398.5 41 165067.0 42 171637.0 43 175866.5 44 183867.5 45 192784.0 46 193845.5 47 190300.5 48 186839.0 49 182139.5 50 177452.5 51 166749.5 52 156724.0 53 153213.0 54 146471.0 55 133803.5 56 119664.5 57 108129.0 58 96838.5 59 85292.5 60 72592.0 61 58850.5 62 48155.0 63 38144.0 64 29520.5 65 23344.5 66 17273.5 67 13262.0 68 10052.5 69 6615.5 70 4751.0 71 3550.0 72 2903.0 73 2561.5 74 2056.0 75 1678.0 76 1110.5 77 676.0 78 533.0 79 366.0 80 211.5 81 141.0 82 106.0 83 76.0 84 58.5 85 45.0 86 41.5 87 36.5 88 22.5 89 13.0 90 12.5 91 12.0 92 9.0 93 8.5 94 7.0 95 5.5 96 6.5 97 5.5 98 4.5 99 9.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.250446276116066E-4 2 1.2189173535435247E-4 3 4.0630578451450824E-5 4 2.4378347070870494E-4 5 7.516657013518403E-4 6 4.875669414174099E-4 7 5.688280983203116E-4 8 9.34503304383369E-4 9 0.0011986020643177994 10-11 0.0012087597089306622 12-13 0.0014119126011879163 14-15 0.0019096371872181887 16-17 0.0017471148734123855 18-19 0.0016556960718966212 20-21 0.001970583054895365 22-23 0.09133754035886146 24-25 0.0014627008242522298 26-27 0.001107183262802035 28-29 0.0010563950397377215 30-31 8.32926858254742E-4 32-33 0.0011986020643177994 34-35 7.211927675132522E-4 36-37 4.875669414174099E-4 38-39 7.110351229003895E-4 40-41 9.446609489962317E-4 42-43 9.954491720605453E-4 44-45 9.548185936090944E-4 46-47 0.0011681291304792112 48-49 2.742564045472931E-4 50-51 9.34503304383369E-4 52-53 8.837150813190554E-4 54-55 8.735574367061928E-4 56-57 7.922962798032911E-4 58-59 4.875669414174099E-4 60-61 7.313504121261149E-4 62-63 8.430845028676047E-4 64-65 6.805621890618013E-4 66-67 3.148869829987439E-4 68-69 1.422070245800779E-4 70-71 4.570940075788218E-4 72-73 2.0315289225725415E-4 74-75 2.5394111532156766E-4 76-77 2.844140491601558E-4 78-79 2.0315289225725415E-4 80-81 3.7583285067592016E-4 82-83 1.3204937996721519E-4 84-85 2.945716937730185E-4 86-87 2.844140491601558E-4 88-89 3.45359916837332E-4 90-91 2.4378347070870494E-4 92-93 2.742564045472931E-4 94-95 2.6409875993443037E-4 96-97 3.047293383858812E-4 98-99 4.063057845145083E-4 100-101 4.16463429127371E-4 102-103 6.094586767717624E-4 104-105 4.570940075788218E-4 106-107 4.672516521916845E-4 108-109 4.875669414174099E-4 110-111 7.211927675132522E-4 112-113 4.2662107374023367E-4 114-115 2.6409875993443037E-4 116-117 7.618233459647031E-4 118-119 9.446609489962317E-4 120-121 6.094586767717624E-4 122-123 5.586704537074489E-4 124-125 3.047293383858812E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4922401.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.248038416144457 #Duplication Level Percentage of deduplicated Percentage of total 1 50.83341461043922 14.35944248739486 2 13.729776843312816 7.75678527429982 3 9.955771376094132 8.436930368827754 4 7.053482851957743 7.96988218278874 5 4.876985046001793 6.888263046721034 6 3.3634112974046886 5.700586292302915 7 2.3102517912624325 4.568205694538342 8 1.6095840183118584 3.637407294662843 9 1.1095154356171801 2.8207471183757424 >10 4.75908825156708 22.275589116999914 >50 0.22950608695652552 4.496254306208698 >100 0.1583358712489544 8.51418837001843 >500 0.007903605383048053 1.4904844394540049 >1k 0.0029729144426814957 1.085234007406991 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.0315289225725412E-5 0.0 4 0.0 0.0 0.0 2.0315289225725412E-5 0.0 5 0.0 0.0 0.0 2.0315289225725412E-5 0.0 6 0.0 0.0 0.0 2.0315289225725412E-5 0.0 7 0.0 0.0 0.0 2.0315289225725412E-5 0.0 8 0.0 0.0 0.0 2.0315289225725412E-5 0.0 9 0.0 0.0 0.0 2.0315289225725412E-5 0.0 10-11 0.0 0.0 0.0 2.0315289225725412E-5 4.0630578451450824E-5 12-13 0.0 0.0 0.0 2.0315289225725412E-5 4.0630578451450824E-5 14-15 0.0 0.0 0.0 3.0472933838588118E-5 4.0630578451450824E-5 16-17 0.0 0.0 0.0 4.0630578451450824E-5 4.0630578451450824E-5 18-19 0.0 0.0 0.0 1.0157644612862707E-4 4.0630578451450824E-5 20-21 0.0 0.0 0.0 1.422070245800779E-4 4.0630578451450824E-5 22-23 0.0 0.0 0.0 1.9299524764439143E-4 4.0630578451450824E-5 24-25 0.0 0.0 0.0 2.5394111532156766E-4 4.0630578451450824E-5 26-27 0.0 0.0 0.0 3.5551756145019473E-4 4.0630578451450824E-5 28-29 0.0 0.0 0.0 5.485128090945862E-4 4.0630578451450824E-5 30-31 0.0 0.0 0.0 0.0016861690057352093 4.0630578451450824E-5 32-33 0.0 0.0 0.0 0.005414024578655823 4.0630578451450824E-5 34-35 0.0 0.0 0.0 0.011193724363374702 4.0630578451450824E-5 36-37 0.0 0.0 0.0 0.020975536125561488 4.0630578451450824E-5 38-39 0.0 0.0 0.0 0.03891393651187702 4.0630578451450824E-5 40-41 2.0315289225725412E-5 0.0 0.0 0.057461795574964336 4.0630578451450824E-5 42-43 2.0315289225725412E-5 0.0 0.0 0.08058059471383985 4.0630578451450824E-5 44-45 2.0315289225725412E-5 0.0 0.0 0.10944862070359566 4.0630578451450824E-5 46-47 2.0315289225725412E-5 0.0 0.0 0.14807814316631254 4.0630578451450824E-5 48-49 2.0315289225725412E-5 0.0 0.0 0.18794689827179867 4.0630578451450824E-5 50-51 2.0315289225725412E-5 0.0 0.0 0.22801880626954205 4.0630578451450824E-5 52-53 2.0315289225725412E-5 0.0 0.0 0.27203188037707615 4.0630578451450824E-5 54-55 2.0315289225725412E-5 0.0 0.0 0.3259385003375385 4.0630578451450824E-5 56-57 2.0315289225725412E-5 0.0 0.0 0.40067844939898234 4.0630578451450824E-5 58-59 2.0315289225725412E-5 0.0 0.0 0.4857791959655461 4.0630578451450824E-5 60-61 2.0315289225725412E-5 0.0 0.0 0.5650900851027781 4.0630578451450824E-5 62-63 2.0315289225725412E-5 0.0 0.0 0.6492766436541842 4.0630578451450824E-5 64-65 2.0315289225725412E-5 0.0 0.0 0.7417010519866221 4.0630578451450824E-5 66-67 2.0315289225725412E-5 0.0 0.0 0.8573966241271282 4.0630578451450824E-5 68-69 2.0315289225725412E-5 0.0 0.0 1.011000525962838 4.0630578451450824E-5 70-71 2.0315289225725412E-5 0.0 0.0 1.1627760517682326 4.0630578451450824E-5 72-73 2.0315289225725412E-5 0.0 0.0 1.3084265178720709 5.078822306431354E-5 74-75 2.0315289225725412E-5 0.0 0.0 1.4654433882977027 6.094586767717624E-5 76-77 2.0315289225725412E-5 0.0 0.0 1.6531465030987926 6.094586767717624E-5 78-79 2.0315289225725412E-5 0.0 0.0 1.894603873191152 6.094586767717624E-5 80-81 2.0315289225725412E-5 0.0 0.0 2.1439131025692544 6.094586767717624E-5 82-83 2.0315289225725412E-5 0.0 0.0 2.388234928442441 6.094586767717624E-5 84-85 2.0315289225725412E-5 0.0 0.0 2.6384177152572494 7.110351229003895E-5 86-87 2.0315289225725412E-5 0.0 0.0 2.9167168623604622 8.126115690290165E-5 88-89 2.0315289225725412E-5 0.0 0.0 3.2583692389140992 8.126115690290165E-5 90-91 2.0315289225725412E-5 0.0 0.0 3.625730613982892 8.126115690290165E-5 92-93 2.0315289225725412E-5 0.0 0.0 3.9848541392706527 8.126115690290165E-5 94-95 2.0315289225725412E-5 0.0 0.0 4.356237941606139 8.126115690290165E-5 96-97 2.0315289225725412E-5 0.0 0.0 4.7471345792429345 8.126115690290165E-5 98-99 2.0315289225725412E-5 0.0 0.0 5.202796358931343 8.126115690290165E-5 100-101 2.0315289225725412E-5 0.0 0.0 5.711988925729537 8.126115690290165E-5 102-103 2.0315289225725412E-5 0.0 0.0 6.2081289192002025 8.126115690290165E-5 104-105 2.0315289225725412E-5 0.0 0.0 6.723720802104502 8.126115690290165E-5 106-107 2.0315289225725412E-5 0.0 0.0 7.25389906267287 8.126115690290165E-5 108-109 2.0315289225725412E-5 0.0 0.0 7.807439905850824 8.126115690290165E-5 110-111 2.0315289225725412E-5 0.0 0.0 8.412226066100668 8.126115690290165E-5 112-113 2.0315289225725412E-5 0.0 0.0 9.027586334392506 8.126115690290165E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6355 0.0 57.10681 1 GTACATG 34250 0.0 54.682426 1 TACATGG 35245 0.0 52.514126 2 ACATGGG 35115 0.0 51.522552 3 CATGGGA 22605 0.0 46.979385 4 CATGGGG 12590 0.0 43.049316 4 GTATCAA 8795 0.0 41.06071 2 ATGGGAT 9980 0.0 37.675602 5 ATGGGAG 9165 0.0 37.131046 5 ATCAACG 10015 0.0 35.46476 4 TCAACGC 10080 0.0 35.29509 5 CAACGCA 10340 0.0 34.580204 6 AACGCAG 10600 0.0 34.01264 7 ATGGGGG 7295 0.0 32.78494 5 GAGTACA 20045 0.0 31.75787 1 AGTACAT 19565 0.0 31.107807 2 TACCTGG 4115 0.0 30.36149 2 TATCAAC 12220 0.0 29.893087 3 ATGGGGA 7020 0.0 29.492783 5 ACGCAGA 12850 0.0 26.992249 8 >>END_MODULE