FastQCFastQC Report
Thu 26 May 2016
SRR936239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4922401
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131990.26814150249034974No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111290.22608885379309815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104920.21314801455631105No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108450.079.378031
GTATCAA166000.051.6076972
GTACATG312050.048.205791
ACATGGG318050.045.9347043
TACATGG324050.045.8741762
CATGGGA207500.040.941454
CATGGGG112800.038.3164144
ATCAACG233350.037.7326854
TCAACGC233500.037.6829535
CAACGCA238700.036.9377756
AACGCAG243650.036.2605977
TATCAAC252950.034.9975853
ATGGGAT96200.032.05625
ATGGGAG85850.031.7601495
ACGCAGA282950.031.1190956
CGCAGAG287400.030.5544537
GAGTACA208150.029.5941681
AGTACAT198550.029.354492
TACCTGG45500.028.1312392
ATGGGGG67800.027.8347235
Produced by FastQC (version 0.11.5)