FastQCFastQC Report
Thu 26 May 2016
SRR936238_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936238_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4053053
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG139860.34507320777695233No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG112030.27640891939977097No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA102570.25306848935851567No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA99040.24435900542134534No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA91190.2249908895837286No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA84100.2074979034323015No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA81210.20036747607297514No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA77080.1901776265940761No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA76340.1883518424259441No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA72320.17843339329636204No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG70490.17391827839408958No Hit
GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG60260.14867804590761582No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG59740.14739506243811765No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT58850.14519918688455344No Hit
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT57370.1415476185482894No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA55100.13594690224874928No Hit
GCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTT46040.1135933825686464No Hit
CCTCTTAGGAGTGGGGGTGGCTTTTGGGAGGGTGAGGGACTTCCTGTAAC45780.11295189083389733No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT44590.1100158325094688No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT44030.10863415800385537No Hit
TCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCC43900.10831341213648082No Hit
GTACATAATTTACACAGAAGCAATGCTGTCACCTTCCCCGGGGTGGACTC43600.1075732293656165No Hit
CCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAA43100.10633959141417593No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42800.058.7987171
GTACATG184700.050.60351
TACATGG185050.049.3825342
ACATGGG185100.047.408573
GTATCAA55450.044.6337172
CATGGGG108900.039.3893364
TCAACGC65350.037.4168745
CAACGCA65800.037.25146
CATGGGA76400.037.0667764
ATCAACG66700.036.5703664
AACGCAG68700.036.025327
GAGTACA108550.032.6105731
TATCAAC77350.032.3042953
GTGTAAG18750.028.5569951
ACGCAGA84050.028.525858
TAAGGTA13600.027.9970514
CGCAGAG86000.027.3947939
ATGGGGC48900.026.2794425
ATGGGGA61250.026.0315485
AGGTAAG23250.025.5887052
Produced by FastQC (version 0.11.5)