##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936238_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4053053 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.74070139225912 31.0 30.0 34.0 26.0 34.0 2 29.710542151805072 31.0 30.0 34.0 25.0 34.0 3 29.703773427093108 31.0 30.0 34.0 25.0 34.0 4 33.154358948674 35.0 35.0 37.0 26.0 37.0 5 33.25288517075893 35.0 35.0 37.0 27.0 37.0 6 33.312174304160344 37.0 35.0 37.0 27.0 37.0 7 33.12283727846638 36.0 35.0 37.0 26.0 37.0 8 33.0661691322566 36.0 35.0 37.0 26.0 37.0 9 34.395966201280864 38.0 35.0 39.0 26.0 39.0 10-11 34.24429041515124 38.0 34.5 39.0 25.5 39.0 12-13 34.27319282526037 38.0 35.0 39.0 25.0 39.0 14-15 35.41598987232587 39.0 35.0 41.0 25.5 41.0 16-17 35.43670845162893 39.0 35.0 41.0 25.5 41.0 18-19 35.4312751399994 39.0 35.0 41.0 25.5 41.0 20-21 35.496815610355945 39.0 35.0 41.0 26.0 41.0 22-23 35.41413115002444 39.0 35.0 41.0 25.5 41.0 24-25 35.36914937949245 39.0 34.0 41.0 25.5 41.0 26-27 35.43879206612892 39.0 35.0 41.0 27.0 41.0 28-29 35.31993598405943 39.0 34.0 41.0 26.0 41.0 30-31 35.22203731360039 39.0 34.0 41.0 26.0 41.0 32-33 35.029625321948664 39.0 34.0 41.0 25.0 41.0 34-35 35.0044584909203 39.0 34.0 40.5 25.0 41.0 36-37 34.89391688191593 39.0 34.0 40.0 25.0 41.0 38-39 34.629052963284714 38.0 33.0 40.0 24.0 41.0 40-41 34.7051200662809 38.5 33.5 40.0 24.5 41.0 42-43 34.59976084694674 38.5 33.0 40.5 24.0 41.0 44-45 34.481645811194674 38.0 33.0 40.0 24.0 41.0 46-47 34.41469282538373 38.0 33.0 40.0 24.0 41.0 48-49 34.27780761810911 38.0 33.0 40.0 23.0 41.0 50-51 34.103293615948274 38.0 33.0 40.0 23.0 41.0 52-53 33.90027529864524 38.0 33.0 40.0 22.5 41.0 54-55 33.69472543290206 38.0 33.0 40.0 22.0 41.0 56-57 33.45424745247595 37.0 32.0 40.0 21.5 41.0 58-59 33.178846662010095 37.0 32.0 40.0 20.0 41.0 60-61 32.89496855826953 36.0 32.0 40.0 18.5 41.0 62-63 32.609267754455715 36.0 31.0 39.5 18.0 41.0 64-65 32.286844756286186 35.5 31.0 39.0 17.5 41.0 66-67 31.96621053314625 35.0 31.0 39.0 14.5 40.5 68-69 31.658176441314733 35.0 31.0 38.5 13.0 40.0 70-71 31.29897166407644 35.0 30.5 37.5 10.5 40.0 72-73 31.011123836771937 35.0 30.0 37.0 8.0 39.5 74-75 30.69099959956112 35.0 30.0 36.5 7.0 39.0 76-77 30.402719998973613 34.0 30.0 36.0 7.0 39.0 78-79 30.084586730052628 34.0 30.0 36.0 3.5 38.5 80-81 29.828517416377235 34.0 29.5 35.0 2.0 37.0 82-83 29.548016396528737 34.0 29.0 35.0 2.0 37.0 84-85 29.621149785112607 34.0 30.0 35.0 2.0 36.5 86-87 29.54596522670688 34.0 30.0 35.0 2.0 36.0 88-89 29.382643651588076 34.0 30.0 35.0 2.0 36.0 90-91 29.227658631653718 34.0 30.0 35.0 2.0 36.0 92-93 29.12262213200765 34.0 29.5 35.0 2.0 35.0 94-95 28.99417772232438 34.0 29.0 35.0 2.0 35.0 96-97 28.89631198999865 34.0 29.0 35.0 2.0 35.0 98-99 28.766403252067022 34.0 29.0 35.0 2.0 35.0 100-101 28.649563674592954 34.0 29.0 35.0 2.0 35.0 102-103 27.655147613416354 32.5 26.5 34.5 2.0 35.0 104-105 28.320176543459954 34.0 29.0 35.0 2.0 35.0 106-107 28.43275858963601 34.0 29.0 35.0 2.0 35.0 108-109 28.371017970897494 34.0 29.0 35.0 2.0 35.0 110-111 28.227144574719354 34.0 29.0 35.0 2.0 35.0 112-113 28.160567231664622 34.0 29.0 35.0 2.0 35.0 114-115 28.010542423205422 34.0 29.0 35.0 2.0 35.0 116-117 27.85883554939943 34.0 28.5 35.0 2.0 35.0 118-119 27.60403120807944 34.0 27.0 35.0 2.0 35.0 120-121 27.381227928675003 34.0 27.0 35.0 2.0 35.0 122-123 27.065417846744168 34.0 27.0 35.0 2.0 35.0 124-125 25.103918823662063 31.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5044379707741733 1101 2 0.5435525578643592 1101 3 0.4774246016812853 1101 4 0.5703383671309012 1101 5 0.5691655404523459 1101 6 0.48457342202316767 1101 7 0.5750055137679766 1101 8 0.5469871732752907 1101 9 0.6208771655963474 1101 10-11 0.6996269507741246 1101 12-13 0.664452319345223 1101 14-15 0.8398787602068936 1101 16-17 0.8154817116044129 1101 18-19 0.7448418746482091 1101 20-21 0.7178458015233318 1101 22-23 0.7850431526784476 1101 24-25 0.8195442688363315 1101 26-27 0.7352991522907857 1101 28-29 0.7884562461998001 1101 30-31 0.8425089190927721 1101 32-33 0.8952436870979383 1101 34-35 0.7994481132562896 1101 36-37 0.689978019398616 1101 38-39 0.8982331293911656 1101 40-41 0.8251372278867564 1101 42-43 0.8829188087407616 1101 44-45 0.8452333324039287 1101 46-47 0.7562819040587456 1101 48-49 0.7656220328141643 1101 50-51 0.8152089825466078 1101 52-53 0.7766599476023259 1101 54-55 0.7507112258922035 1101 56-57 0.7605091270904083 1101 58-59 0.7918838438861187 1101 60-61 0.7825356328573463 1101 62-63 0.7433760075367957 1101 64-65 0.694409049196814 1101 66-67 0.704178357819714 1101 68-69 0.7272592777793321 1101 70-71 0.7632794146959867 1101 72-73 0.729799394063896 1101 74-75 0.7354625585478161 1101 76-77 0.6598962366111607 1101 78-79 0.7346624513596538 1101 80-81 0.7626588774980974 1101 82-83 0.7637150495405081 1101 84-85 0.4542473257163486 1101 86-87 0.6021808675106897 1101 88-89 0.5886638072147896 1101 90-91 0.5903863725165763 1101 92-93 0.6395265668304333 1101 94-95 0.6971705190253381 1101 96-97 0.7232893118026595 1101 98-99 0.7351217017557055 1101 100-101 0.7101070243834187 1101 102-103 0.7803718278953546 1101 104-105 0.7388072581441314 1101 106-107 0.7206133530018093 1101 108-109 0.631779719349467 1101 110-111 0.6650011472332871 1101 112-113 0.7333016546666471 1101 114-115 0.7354499518108817 1101 116-117 0.7586930100996128 1101 118-119 0.7196699275262262 1101 120-121 0.7277750315159892 1101 122-123 0.6976539912428876 1101 124-125 0.5971224470824197 1102 1 0.4033703096606267 1102 2 0.3248562466577454 1102 3 0.47464951070485384 1102 4 0.5039488293867791 1102 5 0.3821981788915423 1102 6 0.3648717267800805 1102 7 0.34410299314602355 1102 8 0.4057282311723327 1102 9 0.555759545567355 1102 10-11 0.6484214436244073 1102 12-13 0.4527736700397753 1102 14-15 0.694061209329881 1102 16-17 0.5810381041693162 1102 18-19 0.592347818140432 1102 20-21 0.5417742419868787 1102 22-23 0.6509180985981331 1102 24-25 0.795591320511285 1102 26-27 0.5728761514857084 1102 28-29 0.5878658106644465 1102 30-31 0.5815870278576156 1102 32-33 0.6238751458871761 1102 34-35 0.6176832810240143 1102 36-37 0.5466044041935376 1102 38-39 0.7422841116044694 1102 40-41 0.5221309668424965 1102 42-43 0.6434683685334122 1102 44-45 0.6760903907596258 1102 46-47 0.5671903568775676 1102 48-49 0.5284970308756414 1102 50-51 0.4172626973803588 1102 52-53 0.5613424486839307 1102 54-55 0.5350282826433528 1102 56-57 0.5926962650667349 1102 58-59 0.5973884048272353 1102 60-61 0.6270222859638963 1102 62-63 0.5687083442740786 1102 64-65 0.5693808077634799 1102 66-67 0.5403566504973156 1102 68-69 0.4699033353433819 1102 70-71 0.406374065229393 1102 72-73 0.4017118583812689 1102 74-75 0.3422559619155905 1102 76-77 0.37865061203374495 1102 78-79 0.33924748515229197 1102 80-81 0.4374241692375058 1102 82-83 0.38541519176425254 1102 84-85 0.46027446460119137 1102 86-87 0.4152436675930957 1102 88-89 0.48849021291526284 1102 90-91 0.4829269097039912 1102 92-93 0.5295651285523313 1102 94-95 0.42595003851250723 1102 96-97 0.51484474774281 1102 98-99 0.5031339603267106 1102 100-101 0.5158732833713131 1102 102-103 0.5296422275151471 1102 104-105 0.5150576383701093 1102 106-107 0.4300428473522402 1102 108-109 0.4825981805708146 1102 110-111 0.5378661821835529 1102 112-113 0.5238160376797083 1102 114-115 0.4969565393213493 1102 116-117 0.5328933510605012 1102 118-119 0.5168461505289628 1102 120-121 0.530711578867507 1102 122-123 0.3954177435173776 1102 124-125 0.32462051191383523 1103 1 0.2538514808792378 1103 2 0.3016339916414488 1103 3 0.3202032393794809 1103 4 0.2317022360707881 1103 5 0.1376854413618318 1103 6 0.1940266691853907 1103 7 0.17875084758051685 1103 8 0.21478478222962138 1103 9 0.3495196591337617 1103 10-11 0.3720662514058759 1103 12-13 0.30290431180912236 1103 14-15 0.3445811228265754 1103 16-17 0.26967293791135205 1103 18-19 0.3023707690454174 1103 20-21 0.29099580125143376 1103 22-23 0.31790912983779407 1103 24-25 0.3760733100599438 1103 26-27 0.28832062453935947 1103 28-29 0.39229186341695055 1103 30-31 0.35685176961114706 1103 32-33 0.5247853069441177 1103 34-35 0.43525457332123807 1103 36-37 0.42538875265558573 1103 38-39 0.5033664917601968 1103 40-41 0.46353622442471476 1103 42-43 0.6148602863087049 1103 44-45 0.5554061401547798 1103 46-47 0.42038074838172435 1103 48-49 0.3580262137576824 1103 50-51 0.43149881538697343 1103 52-53 0.3704255355481294 1103 54-55 0.42644581517625113 1103 56-57 0.3944651419549743 1103 58-59 0.34848553204365373 1103 60-61 0.39118530210245694 1103 62-63 0.33582856209368117 1103 64-65 0.29490871624199855 1103 66-67 0.34254179715643573 1103 68-69 0.34335184663325435 1103 70-71 0.33234164634849606 1103 72-73 0.33278287436370135 1103 74-75 0.36648392401355423 1103 76-77 0.33059015696006355 1103 78-79 0.2397348708856839 1103 80-81 0.29470621096754357 1103 82-83 0.2835745312383722 1103 84-85 0.34995621296504353 1103 86-87 0.3293309967624154 1103 88-89 0.36670412712825495 1103 90-91 0.41839413849605833 1103 92-93 0.3758073330170575 1103 94-95 0.44963889573342897 1103 96-97 0.41407202504575835 1103 98-99 0.2875105845748891 1103 100-101 0.3599146422511552 1103 102-103 0.3558748071859128 1103 104-105 0.3887360695869013 1103 106-107 0.3894074534478733 1103 108-109 0.2587398023434595 1103 110-111 0.20196190009183113 1103 112-113 0.2755384683459283 1103 114-115 0.2718954898455941 1103 116-117 0.2707291659768458 1103 118-119 0.1780265248516102 1103 120-121 0.24404123371027708 1103 122-123 0.28344502712043607 1103 124-125 0.23268806488786709 1104 1 0.2366701177467796 1104 2 0.3226876580386282 1104 3 0.32884047225940094 1104 4 0.4013822655179311 1104 5 0.23350807967815967 1104 6 0.29499705783582897 1104 7 0.10846317617543377 1104 8 0.09483658186019994 1104 9 0.27830498804530635 1104 10-11 0.32966335705339134 1104 12-13 0.3630142357635293 1104 14-15 0.3782042331771507 1104 16-17 0.34627489235941766 1104 18-19 0.2917518644194388 1104 20-21 0.35208806595516506 1104 22-23 0.35978419711131693 1104 24-25 0.4428131133580564 1104 26-27 0.30299764484673375 1104 28-29 0.3098859717098392 1104 30-31 0.38024792954024633 1104 32-33 0.5417053836418262 1104 34-35 0.4868182064136022 1104 36-37 0.4456456451963007 1104 38-39 0.5508411713811014 1104 40-41 0.48653178771132843 1104 42-43 0.6305526457649862 1104 44-45 0.5440228708147714 1104 46-47 0.49618121491624123 1104 48-49 0.39116299055257997 1104 50-51 0.43008376348127797 1104 52-53 0.41991169350518476 1104 54-55 0.4080983612777658 1104 56-57 0.414895128779186 1104 58-59 0.4588852624781552 1104 60-61 0.5004607299227786 1104 62-63 0.4778568165804771 1104 64-65 0.5830749008963281 1104 66-67 0.509051868739391 1104 68-69 0.4164493906880793 1104 70-71 0.39566149944276674 1104 72-73 0.46936609829162634 1104 74-75 0.4709462013431036 1104 76-77 0.3932674742530331 1104 78-79 0.28091829759663156 1104 80-81 0.4015773714662636 1104 82-83 0.44325182260402585 1104 84-85 0.4712589681742756 1104 86-87 0.46226986172746365 1104 88-89 0.48813592965011665 1104 90-91 0.49402858183270126 1104 92-93 0.4766845699353546 1104 94-95 0.5371253133564586 1104 96-97 0.4945398009300561 1104 98-99 0.5625370716831206 1104 100-101 0.47966382102312366 1104 102-103 0.4630156019287952 1104 104-105 0.48184125041420245 1104 106-107 0.5328050924123815 1104 108-109 0.5676722432272179 1104 110-111 0.5680360785298895 1104 112-113 0.5060048889388113 1104 114-115 0.49890783242555514 1104 116-117 0.5518755303781688 1104 118-119 0.5882018858108893 1104 120-121 0.6040636431591295 1104 122-123 0.6598995101494367 1104 124-125 0.636540745889981 1105 1 0.3129447732665689 1105 2 0.3536078135631513 1105 3 0.41100388875182503 1105 4 0.2908604725107793 1105 5 0.1792671921171234 1105 6 0.2973415772716592 1105 7 0.28181194544523436 1105 8 0.27599242115023515 1105 9 0.35084393825477633 1105 10-11 0.4880614431595802 1105 12-13 0.4177140615307664 1105 14-15 0.42949734670320794 1105 16-17 0.330115055203386 1105 18-19 0.3250711018453387 1105 20-21 0.1758695984227998 1105 22-23 0.11305342836362087 1105 24-25 0.3398685348140944 1105 26-27 0.2726698749043166 1105 28-29 0.34619581917453246 1105 30-31 0.40719338214641 1105 32-33 0.5215023083766326 1105 34-35 0.5126273153648242 1105 36-37 0.3800848213074133 1105 38-39 0.5650140797619514 1105 40-41 0.3717632040592207 1105 42-43 0.5635388879081376 1105 44-45 0.6128406318900161 1105 46-47 0.38998710219419763 1105 48-49 0.36205854055479136 1105 50-51 0.356563691256909 1105 52-53 0.42706646550605143 1105 54-55 0.4746956667083495 1105 56-57 0.41597619744412384 1105 58-59 0.3887816121501828 1105 60-61 0.42897975775538555 1105 62-63 0.39615885970448517 1105 64-65 0.44729414027381864 1105 66-67 0.5356535343365465 1105 68-69 0.507850529683779 1105 70-71 0.40947941365037366 1105 72-73 0.3689411148946533 1105 74-75 0.4109411962815237 1105 76-77 0.2859459787767662 1105 78-79 0.3365460838087664 1105 80-81 0.37689964265219444 1105 82-83 0.41623221914827724 1105 84-85 0.4296112015205722 1105 86-87 0.43471354621024005 1105 88-89 0.37475591123172336 1105 90-91 0.4118456131862871 1105 92-93 0.40286982658231096 1105 94-95 0.33158995484990683 1105 96-97 0.37173498007175 1105 98-99 0.3880660522504762 1105 100-101 0.4324580013552861 1105 102-103 0.3887811575210627 1105 104-105 0.32969510744672803 1105 106-107 0.38653866498003 1105 108-109 0.4013889026382138 1105 110-111 0.43484990927195355 1105 112-113 0.2816104815842877 1105 114-115 0.3114582646646191 1105 116-117 0.33961145326205866 1105 118-119 0.37250668947200083 1105 120-121 0.3677732053435321 1105 122-123 0.4480945644665084 1105 124-125 0.45578920988049276 1106 1 -0.31005200285827783 1106 2 -0.5227019712038867 1106 3 -0.27560876270851864 1106 4 -0.30711975230862265 1106 5 -0.23160210236173384 1106 6 -0.26206243175567323 1106 7 -0.45034253180586603 1106 8 -0.48528390706353264 1106 9 -0.38522780526538014 1106 10-11 -0.358203480910781 1106 12-13 -0.3993904618528319 1106 14-15 -0.5350910663031527 1106 16-17 -0.45840893309132724 1106 18-19 -0.5221531380484024 1106 20-21 -0.513446512914264 1106 22-23 -0.4988960862553995 1106 24-25 -4.333554435926267 1106 26-27 -1.3696169647981904 1106 28-29 -0.9941403181023176 1106 30-31 -0.9109427332940001 1106 32-33 -5.738402403136327 1106 34-35 -1.416810252199177 1106 36-37 -0.685336412115781 1106 38-39 -0.32551106274484454 1106 40-41 -0.8901417018221025 1106 42-43 -0.48589605208363196 1106 44-45 -0.49430088147160944 1106 46-47 -0.5574828097041475 1106 48-49 -0.3539177225095784 1106 50-51 -0.3425988570802261 1106 52-53 -0.2486767714949636 1106 54-55 -0.4213795442473014 1106 56-57 -0.31587462124259247 1106 58-59 -0.28776521802586075 1106 60-61 -0.25876618120575046 1106 62-63 -0.23911179823240047 1106 64-65 -0.16231705102423888 1106 66-67 -0.24350866605722743 1106 68-69 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-0.7653756824714613 2216 108-109 -0.7841528254833889 2216 110-111 -0.7357404217839623 2216 112-113 -0.7419648489817803 2216 114-115 -0.7642807442373787 2216 116-117 -0.6045562354012759 2216 118-119 -0.7204582727943247 2216 120-121 -0.7472844566664882 2216 122-123 -0.7115393801713239 2216 124-125 -0.7117738608515936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 131171.0 3 21605.0 4 13652.0 5 11385.0 6 11980.0 7 13523.0 8 14870.0 9 16892.0 10 19420.0 11 22169.0 12 23776.0 13 23653.0 14 23103.0 15 21189.0 16 19106.0 17 17580.0 18 16925.0 19 16376.0 20 17069.0 21 18543.0 22 22086.0 23 28893.0 24 40287.0 25 54021.0 26 63525.0 27 67807.0 28 79416.0 29 95736.0 30 113444.0 31 138546.0 32 174559.0 33 226028.0 34 303732.0 35 437594.0 36 701163.0 37 795714.0 38 235871.0 39 644.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.67503452339214 17.192710968952017 9.405561097336843 24.726693410318997 2 19.545690506808203 19.62521082877792 35.54321533501174 25.285883329402136 3 17.319931419599 25.55002364883953 30.29928303429538 26.830761897266086 4 11.690877657783066 15.950115579653737 35.438505678071 36.920501084492194 5 11.671896893272535 41.011630027117896 34.559490239802535 12.75698283980703 6 33.40337943447457 36.90884261092586 16.712274351752818 12.97550360284675 7 28.594071170722835 33.845187430777315 20.777302387366728 16.783439011133115 8 27.927347337972158 32.86421198123482 19.212107066353685 19.99633361443934 9 26.616057578693653 13.689570782528712 18.260894881776014 41.43347675700162 10-11 24.703915776539883 25.752267903437616 28.443342661750997 21.1004736582715 12-13 25.78207191878838 24.7262779125471 27.367644243200616 22.1240059254639 14-15 22.782456816911996 26.488263213129915 23.970883822112707 26.75839614784538 16-17 23.4078644068633 28.994098826612092 25.11338802111765 22.48464874540696 18-19 23.254020282698466 27.212694463449523 26.51348589974219 23.01979935410982 20-21 23.381011978156934 26.722719761460795 26.336055800607085 23.560212459775194 22-23 23.25501970147013 27.104066632357434 26.378497887611235 23.262415778561206 24-25 22.975458438733067 27.22040156451142 26.37916517857969 23.424974818175816 26-27 22.579971034016527 27.2836248436355 26.16753391906796 23.968870203280016 28-29 23.20249274833819 27.676187964258403 26.002479869105542 23.118839418297863 30-31 22.57277133843798 26.978737924490222 26.133236352582927 24.315254384488867 32-33 22.069910914786085 27.582306025653157 26.030170764856265 24.317612294704492 34-35 23.628215547729337 27.421859926445368 25.987443505396683 22.96248102042861 36-37 23.675080621413734 26.54878173799302 25.64964371879587 24.126493921797383 38-39 22.933919395121073 27.257560215566457 25.65242037309009 24.156100016222382 40-41 23.468281882839257 26.845180473859305 26.32254788598727 23.363989757314172 42-43 23.06562195135792 26.74521111838687 26.168877438481886 24.020289491773323 44-45 23.008323021103568 27.114702054793256 26.70366445100222 23.173310473100965 46-47 24.02982555784136 27.35782626052483 25.349281550561876 23.263066631071933 48-49 22.991528728416107 27.598958463764895 25.424066286845846 23.98544652097315 50-51 23.557542432043903 27.358628012384283 25.894664979208816 23.189164576363 52-53 23.902517556277235 26.778743103166452 26.058505503588357 23.260233836967952 54-55 22.589948026602286 27.714811246386965 26.498858263005374 23.196382464005367 56-57 23.054218355272848 27.911884347465865 25.90327944205789 23.130617855203393 58-59 23.007134142354698 26.823832451897278 26.16324269777414 24.00579070797388 60-61 22.251661004872016 27.434198522665742 26.599208718090487 23.714931754371747 62-63 22.69679615715516 27.301069641816916 26.93687378779232 23.0652604132356 64-65 22.330666119259035 27.849975931664183 26.243738346718747 23.57561960235803 66-67 22.274207751148456 27.873545139205007 26.341711466250977 23.510535643395556 68-69 23.37745359945552 26.74025403828548 26.769923049318038 23.112369312940956 70-71 22.940977854559527 26.91997242074573 26.273323042309038 23.865726682385706 72-73 22.64235575677576 27.462219809772886 26.119157177927733 23.776267255523617 74-75 22.617657131126993 28.021516152781807 26.331615120274915 23.029211595816285 76-77 22.983310706326535 27.486773841165196 26.43632970005903 23.093585752449236 78-79 23.10746891662602 27.51163259480327 26.427461682569504 22.953436806001207 80-81 23.281580094977915 27.675878459356817 26.004716204288254 23.037825241377014 82-83 22.681830546236448 28.038879338473905 26.204237423522148 23.0750526917675 84-85 22.464482911506867 28.018930933230763 26.486911458509383 23.029674696752988 86-87 23.018652619632128 28.39861338991624 25.687247875057057 22.89548611539458 88-89 22.647570094461 28.52797098282934 25.547868474803597 23.276590447906067 90-91 22.468015140694643 28.14264219012322 26.057003237438074 23.332339431744064 92-93 22.5400023069061 27.778788676915333 26.596604160202908 23.08460485597566 94-95 22.090517533836874 27.66857740925412 27.110997177680307 23.1299078792287 96-97 22.794056327950557 27.113926549142004 26.70213789615228 23.38987922675516 98-99 22.31986206920374 27.8671083755012 26.2392590068657 23.57377054842937 100-101 22.24499931903101 27.419068315993293 26.65809780688509 23.677834558090602 102-103 21.90404839030822 26.745398457405955 27.22498873068951 24.125564421596316 104-105 21.63497960485289 27.5075976780435 26.563690654994794 24.29373206210882 106-107 22.173280990819993 27.591254184814535 26.021669622878786 24.213795201486686 108-109 22.070984689775067 27.442627675057974 26.242529378543477 24.24385825662348 110-111 22.19756994779598 27.416108968069775 26.626750954006013 23.759570130128232 112-113 22.051327537778047 27.65269154810262 26.802788074211296 23.493192839908033 114-115 22.12511423501523 28.01846906328274 26.262407945822503 23.594008755879525 116-117 22.181366713405282 27.68890340749418 26.55661668028197 23.573113198818564 118-119 21.63832717271681 28.131431530061274 26.310251057819574 23.919990239402345 120-121 21.837989182435496 28.223088458583412 26.418133755469352 23.520788603511736 122-123 21.886309796312176 28.16003195625605 26.107461793189586 23.84619645424219 124-125 21.67000962362034 27.903219021435348 26.3407284664864 24.08604288845791 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 877.0 1 1070.5 2 1365.0 3 1390.5 4 1284.0 5 1188.0 6 1089.5 7 1035.0 8 1032.5 9 1019.5 10 970.5 11 981.0 12 1038.5 13 1095.0 14 1150.5 15 1215.5 16 1337.0 17 1505.5 18 1799.0 19 2160.5 20 2657.0 21 3716.0 22 5514.5 23 7874.5 24 10716.5 25 14966.0 26 20350.5 27 26163.5 28 32503.5 29 39442.5 30 47157.5 31 58780.5 32 83215.5 33 89477.5 34 78401.0 35 78856.0 36 84580.0 37 92807.5 38 95001.5 39 95269.0 40 100455.0 41 108562.0 42 111584.5 43 118856.0 44 124995.0 45 131870.0 46 139431.0 47 138972.5 48 135723.0 49 130918.0 50 134146.5 51 136369.5 52 135853.5 53 143205.5 54 151105.5 55 149288.5 56 144900.0 57 137009.0 58 130855.5 59 119013.5 60 92718.5 61 75426.0 62 69155.5 63 52320.0 64 34959.0 65 28825.5 66 21913.5 67 15656.5 68 12204.5 69 9098.0 70 6729.0 71 4699.0 72 3329.0 73 2938.0 74 2458.5 75 1866.5 76 1275.0 77 833.5 78 575.5 79 392.0 80 261.5 81 169.5 82 118.0 83 82.0 84 63.0 85 50.0 86 36.0 87 30.5 88 27.0 89 19.0 90 23.0 91 20.0 92 9.5 93 9.0 94 8.0 95 3.0 96 2.0 97 2.0 98 1.5 99 2.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.869103611524448E-5 2 2.467275902881112E-5 3 0.0 4 9.869103611524448E-5 5 2.467275902881112E-4 6 1.9738207223048897E-4 7 2.467275902881112E-4 8 3.700913854321668E-4 9 6.414917347490892E-4 10-11 6.291553552346835E-4 12-13 9.128920840660115E-4 14-15 0.0011966288128973394 16-17 0.001122610535810906 18-19 0.001036255879210067 20-21 0.0012459743309549617 22-23 0.08904398733497934 24-25 7.525191503787392E-4 26-27 5.674734576626558E-4 28-29 4.071005239753835E-4 30-31 3.700913854321668E-4 32-33 5.798098371770613E-4 34-35 2.714003493169223E-4 36-37 1.1102741562965006E-4 38-39 2.5906396980251674E-4 40-41 3.5775500591776126E-4 42-43 4.687824215474113E-4 44-45 4.317732830041946E-4 46-47 4.687824215474113E-4 48-49 3.700913854321668E-5 50-51 3.330822468889501E-4 52-53 3.8242776494657236E-4 54-55 4.4410966251860024E-4 56-57 3.9476414446097793E-4 58-59 1.9738207223048897E-4 60-61 2.3439121077370564E-4 62-63 3.454186264033557E-4 64-65 2.467275902881112E-4 66-67 4.934551805762224E-5 68-69 6.16818975720278E-5 70-71 1.603729336872723E-4 72-73 7.401827708643336E-5 74-75 1.233637951440556E-4 76-77 1.3570017465846116E-4 78-79 4.934551805762224E-5 80-81 1.3570017465846116E-4 82-83 1.233637951440556E-5 84-85 9.869103611524448E-5 86-87 7.401827708643336E-5 88-89 1.1102741562965006E-4 90-91 6.16818975720278E-5 92-93 1.3570017465846116E-4 94-95 9.869103611524448E-5 96-97 7.401827708643336E-5 98-99 1.480365541728667E-4 100-101 1.233637951440556E-4 102-103 2.467275902881112E-4 104-105 1.3570017465846116E-4 106-107 1.480365541728667E-4 108-109 2.467275902881112E-4 110-111 3.08409487860139E-4 112-113 1.1102741562965006E-4 114-115 1.233637951440556E-4 116-117 3.9476414446097793E-4 118-119 5.551370781482502E-4 120-121 3.700913854321668E-4 122-123 3.08409487860139E-4 124-125 1.1102741562965006E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4053053.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.528054292461178 #Duplication Level Percentage of deduplicated Percentage of total 1 52.41336352437275 12.855978261763191 2 12.743741286969046 6.251583563517116 3 9.078477463231152 6.6803216433305685 4 6.58419270257106 6.459897443227586 5 4.58767024187696 5.626331238433329 6 3.2460643071258346 4.777178493720171 7 2.1751976994987245 3.7347357089098954 8 1.618099973309948 3.17510751967811 9 1.2396502416131607 2.73655875869552 >10 5.806226205463043 24.7042861768988 >50 0.31156312814922404 5.241002120378631 >100 0.17142239977296653 7.7604485016309175 >500 0.01394695887187977 2.3674248302137966 >1k 0.008755025264508555 4.389137211987667 >5k 0.0013234340516117586 2.3549147565088604 >10k+ 3.05407858064252E-4 0.8850937711057595 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 13986 0.34507320777695233 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 11203 0.27640891939977097 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 10257 0.25306848935851567 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 9904 0.24435900542134534 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 9119 0.2249908895837286 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 8410 0.2074979034323015 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 8121 0.20036747607297514 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 7708 0.1901776265940761 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 7634 0.1883518424259441 No Hit CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA 7232 0.17843339329636204 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 7049 0.17391827839408958 No Hit GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG 6026 0.14867804590761582 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 5974 0.14739506243811765 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 5885 0.14519918688455344 No Hit CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT 5737 0.1415476185482894 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 5510 0.13594690224874928 No Hit GCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTT 4604 0.1135933825686464 No Hit CCTCTTAGGAGTGGGGGTGGCTTTTGGGAGGGTGAGGGACTTCCTGTAAC 4578 0.11295189083389733 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 4459 0.1100158325094688 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 4403 0.10863415800385537 No Hit TCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCC 4390 0.10831341213648082 No Hit GTACATAATTTACACAGAAGCAATGCTGTCACCTTCCCCGGGGTGGACTC 4360 0.1075732293656165 No Hit CCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAA 4310 0.10633959141417593 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.7009138543216683E-4 0.0 0.0 0.0 0.0 7 3.7009138543216683E-4 0.0 0.0 0.0 0.0 8 3.7009138543216683E-4 0.0 0.0 0.0 0.0 9 3.7009138543216683E-4 0.0 0.0 0.0 0.0 10-11 3.7009138543216683E-4 0.0 0.0 0.0 0.0 12-13 3.7009138543216683E-4 0.0 0.0 0.0 0.0 14-15 3.7009138543216683E-4 0.0 0.0 0.0 0.0 16-17 5.92146216691467E-4 0.0 0.0 0.0 0.0 18-19 5.92146216691467E-4 0.0 0.0 2.467275902881112E-5 0.0 20-21 5.92146216691467E-4 0.0 0.0 4.934551805762224E-5 0.0 22-23 6.414917347490892E-4 0.0 0.0 9.869103611524448E-5 0.0 24-25 6.908372528067114E-4 0.0 0.0 1.603729336872723E-4 0.0 26-27 6.908372528067114E-4 0.0 0.0 2.590639698025168E-4 0.0 28-29 7.03173632321117E-4 0.0 0.0 4.8111880106181687E-4 0.0 30-31 7.155100118355225E-4 0.0 0.0 0.001714756752502373 0.0 32-33 7.648555298931448E-4 0.0 0.0 0.005551370781482502 0.0 34-35 8.388738069795781E-4 0.0 0.0 0.01089302311122011 0.0 36-37 8.512101864939837E-4 0.0 0.0 0.020860817758859805 0.0 38-39 8.635465660083892E-4 0.0 0.0 0.038551185982517375 0.0 40-41 8.635465660083892E-4 0.0 0.0 0.057586219573245154 0.0 42-43 8.635465660083892E-4 0.0 0.0 0.08293747947534857 0.0 44-45 9.499012226092281E-4 0.0 0.0 0.11553019415240807 0.0 46-47 0.001036255879210067 0.0 0.0 0.15346456115920518 0.0 48-49 0.001036255879210067 0.0 0.0 0.1954822697852705 0.0 50-51 0.001184292433382934 0.0 0.0 0.2400412725913034 0.0 52-53 0.001184292433382934 0.0 0.0 0.2847483119515091 0.0 54-55 0.001184292433382934 0.0 0.0 0.3390160454353792 0.0 56-57 0.001184292433382934 0.0 0.0 0.4079393977823631 0.0 58-59 0.001184292433382934 0.0 0.0 0.4891621205052093 0.0 60-61 0.0015297110597862896 0.0 0.0 0.571556799281924 0.0 62-63 0.001628402095901534 0.0 0.0 0.6556415620521123 0.0 64-65 0.001628402095901534 0.0 0.0 0.7498421560241132 0.0 66-67 0.001628402095901534 0.0 0.0 0.8739214611800044 0.0 68-69 0.001628402095901534 0.0 0.0 1.0178746737336029 0.0 70-71 0.001825784168132023 0.0 0.0 1.1626297509556376 0.0 72-73 0.0021958755535641896 0.0 0.0 1.313910772940793 0.0 74-75 0.0021958755535641896 0.0 0.0 1.475887435964938 0.0 76-77 0.0022575574511362172 0.0 0.0 1.6649917975412607 0.0 78-79 0.0023192393487082453 0.0 0.0 1.8941153742623154 0.0 80-81 0.0023192393487082453 0.0 0.0 2.134008610299446 0.0 82-83 0.0023192393487082453 0.0 0.0 2.387299154489221 0.0 84-85 0.0023192393487082453 0.0 0.0 2.6600935146912708 0.0 86-87 0.0023439121077370563 2.467275902881112E-5 0.0 2.953847383688296 0.0 88-89 0.002442603143852301 2.467275902881112E-5 0.0 3.2778377188751295 0.0 90-91 0.002442603143852301 2.467275902881112E-5 0.0 3.6396884027916734 0.0 92-93 0.002442603143852301 2.467275902881112E-5 0.0 3.9859088938634653 0.0 94-95 0.002442603143852301 4.934551805762224E-5 0.0 4.3622424873299215 0.0 96-97 0.002442603143852301 4.934551805762224E-5 0.0 4.781025562705447 0.0 98-99 0.002491948661909923 4.934551805762224E-5 0.0 5.221816739134672 0.0 100-101 0.002491948661909923 4.934551805762224E-5 0.0 5.711398296543371 0.0 102-103 0.002775685390741251 4.934551805762224E-5 0.0 6.200930508434013 0.0 104-105 0.003059422119572579 4.934551805762224E-5 0.0 6.7158879985038435 0.0 106-107 0.003059422119572579 4.934551805762224E-5 0.0 7.249374730604313 0.0 108-109 0.003059422119572579 4.934551805762224E-5 0.0 7.812764846647699 0.0 110-111 0.0030840948786013903 4.934551805762224E-5 0.0 8.417420152166773 0.0 112-113 0.0030840948786013903 4.934551805762224E-5 0.0 9.025591325847454 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4280 0.0 58.798717 1 GTACATG 18470 0.0 50.6035 1 TACATGG 18505 0.0 49.382534 2 ACATGGG 18510 0.0 47.40857 3 GTATCAA 5545 0.0 44.633717 2 CATGGGG 10890 0.0 39.389336 4 TCAACGC 6535 0.0 37.416874 5 CAACGCA 6580 0.0 37.2514 6 CATGGGA 7640 0.0 37.066776 4 ATCAACG 6670 0.0 36.570366 4 AACGCAG 6870 0.0 36.02532 7 GAGTACA 10855 0.0 32.610573 1 TATCAAC 7735 0.0 32.304295 3 GTGTAAG 1875 0.0 28.556995 1 ACGCAGA 8405 0.0 28.52585 8 TAAGGTA 1360 0.0 27.997051 4 CGCAGAG 8600 0.0 27.394793 9 ATGGGGC 4890 0.0 26.279442 5 ATGGGGA 6125 0.0 26.031548 5 AGGTAAG 2325 0.0 25.588705 2 >>END_MODULE