FastQCFastQC Report
Thu 26 May 2016
SRR936238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4053053
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG154810.38195898252502497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128340.3166501893757619No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG101100.24944159378128042No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99420.24529657026444016No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97200.23981921776004408No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA91860.22664396443865895No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA87540.21598533253821256No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA75390.18600793031820703No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA73910.182356361981943No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA72370.17855675709150606No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA72350.17850741157344846No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG72280.17833470226024678No Hit
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT68000.1677747613959156No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA67400.16629439585418695No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA62600.15445147152035762No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT59480.14675357070336856No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG57300.14137490923508772No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA50100.12361052273434373No Hit
GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG49480.12208081167455742No Hit
TCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCC46090.11371674636379046No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC44020.10860948524482655No Hit
CCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAA43680.10777061143784697No Hit
GCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTT43530.10740052005241481No Hit
GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA42260.10426707965575581No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85650.081.246481
GTATCAA132200.052.3720362
GTACATG165000.044.846311
TACATGG169200.043.24172
ACATGGG169050.042.294033
TCAACGC182600.039.70985
ATCAACG184100.039.4185944
CAACGCA184650.039.2442366
AACGCAG188000.038.6082277
TATCAAC190250.038.2382243
CATGGGG100350.033.6369744
ACGCAGA219650.032.801288
CGCAGAG223550.031.9895179
CATGGGA69150.031.2511234
GAGTACA107000.029.7335361
GTGGTAT24450.029.72831
AGGTAAG22750.026.6913152
TGGTATC26800.026.2118952
TAAGGTG27750.026.172675
CAGAGTA244150.025.61084210-11
Produced by FastQC (version 0.11.5)