##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936238_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4053053 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.566332342557573 31.0 31.0 34.0 30.0 34.0 2 31.330152110026688 31.0 31.0 34.0 28.0 34.0 3 31.691469368893028 31.0 31.0 34.0 30.0 34.0 4 35.53481930781562 37.0 35.0 37.0 33.0 37.0 5 35.13155663150716 37.0 35.0 37.0 33.0 37.0 6 35.33626972062788 37.0 35.0 37.0 33.0 37.0 7 35.1377265483575 37.0 35.0 37.0 32.0 37.0 8 35.1919121215538 37.0 35.0 37.0 32.0 37.0 9 36.852310838274256 39.0 37.0 39.0 32.0 39.0 10-11 36.74289517062817 39.0 37.0 39.0 32.0 39.0 12-13 36.69540652441505 39.0 35.0 39.0 32.0 39.0 14-15 37.845454895359126 40.0 37.0 41.0 32.0 41.0 16-17 37.7532745068964 40.0 37.0 41.0 32.0 41.0 18-19 37.83808353850789 40.0 37.0 41.0 32.0 41.0 20-21 37.87831765338376 40.0 37.0 41.0 32.0 41.0 22-23 37.81661009614234 40.0 37.0 41.0 32.0 41.0 24-25 37.784812461124986 40.0 37.0 41.0 32.0 41.0 26-27 37.59216139043827 40.0 36.5 41.0 32.0 41.0 28-29 37.433107339084884 40.0 36.0 41.0 31.5 41.0 30-31 37.24524574438084 39.0 36.0 41.0 31.0 41.0 32-33 37.04788550754209 39.0 35.5 41.0 31.0 41.0 34-35 36.86769294652697 39.0 35.0 41.0 30.5 41.0 36-37 36.66132665918753 39.0 35.0 41.0 30.0 41.0 38-39 36.63505744928576 39.0 35.0 41.0 30.0 41.0 40-41 36.64117691034388 39.0 35.0 41.0 30.0 41.0 42-43 36.523312919914936 39.0 35.0 41.0 30.0 41.0 44-45 36.40577337626722 39.0 35.0 41.0 30.0 41.0 46-47 36.15709318382957 39.0 35.0 41.0 29.5 41.0 48-49 35.881197087726214 39.0 35.0 41.0 28.5 41.0 50-51 35.659335074078726 39.0 35.0 41.0 28.0 41.0 52-53 35.46333381280728 38.5 34.5 40.5 27.5 41.0 54-55 35.24973199215505 38.0 34.0 40.0 27.0 41.0 56-57 34.99618053353854 38.0 34.0 40.0 26.5 41.0 58-59 34.72833256806659 37.5 34.0 40.0 26.0 41.0 60-61 34.45547751287733 37.0 34.0 40.0 26.0 41.0 62-63 34.186233069244345 36.5 33.0 40.0 26.0 41.0 64-65 33.91309464741764 36.0 33.0 39.0 26.0 41.0 66-67 33.61817684101343 35.5 33.0 39.0 25.5 41.0 68-69 33.29319194691014 35.0 33.0 39.0 25.0 40.5 70-71 32.94863316615894 35.0 32.0 38.0 25.0 40.0 72-73 32.71831888208716 35.0 32.0 37.0 24.5 40.0 74-75 32.58121840499001 35.0 32.0 37.0 25.0 39.0 76-77 32.31661725124246 35.0 32.0 36.0 25.0 39.0 78-79 32.01578082003862 35.0 32.0 36.0 24.0 39.0 80-81 31.796251615757306 35.0 32.0 36.0 24.0 37.0 82-83 31.576270159802995 35.0 32.0 35.0 24.0 37.0 84-85 31.365502498980398 35.0 32.0 35.0 23.5 36.5 86-87 31.198341102373938 35.0 32.0 35.0 23.0 36.0 88-89 31.056061813156653 35.0 32.0 35.0 23.0 36.0 90-91 30.94332617905564 34.5 32.0 35.0 22.5 36.0 92-93 30.824542264806308 34.0 32.0 35.0 20.0 35.0 94-95 30.72262748599636 34.0 31.0 35.0 20.0 35.0 96-97 30.665103441775866 34.0 31.0 35.0 20.0 35.0 98-99 30.564222698296817 34.0 31.0 35.0 20.0 35.0 100-101 30.48180458039902 34.0 31.0 35.0 19.0 35.0 102-103 29.41977134273842 33.5 29.5 34.5 18.0 35.0 104-105 30.208434974820214 34.0 31.0 35.0 19.0 35.0 106-107 30.37259159946835 34.0 31.0 35.0 19.0 35.0 108-109 30.352060779861503 34.0 31.0 35.0 18.5 35.0 110-111 30.29292424747468 34.0 31.0 35.0 18.0 35.0 112-113 30.209012687472875 34.0 31.0 35.0 17.0 35.0 114-115 30.17735321990608 34.0 31.0 35.0 16.0 35.0 116-117 30.066265972835787 34.0 31.0 35.0 11.5 35.0 118-119 29.914117580006973 34.0 31.0 35.0 4.5 35.0 120-121 29.70929889147761 34.0 31.0 35.0 2.0 35.0 122-123 29.47970517533326 34.0 31.0 35.0 2.0 35.0 124-125 27.37381869913865 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15475736471820056 1101 2 0.5922538064241358 1101 3 0.3658306943103149 1101 4 0.22552068017340332 1101 5 0.43820016889752367 1101 6 0.26860318759481316 1101 7 0.4276096726415304 1101 8 0.365031481878205 1101 9 0.3011948709196943 1101 10-11 0.31273780842018795 1101 12-13 0.35617373784203465 1101 14-15 0.435833749619313 1101 16-17 0.45158481047731414 1101 18-19 0.40235758813034295 1101 20-21 0.3450295646012904 1101 22-23 0.3052334045426619 1101 24-25 0.3399400128229928 1101 26-27 0.3939421655556643 1101 28-29 0.45679926462065623 1101 30-31 0.32980597514735877 1101 32-33 0.29470076952191704 1101 34-35 0.2510932993729682 1101 36-37 0.21333387284651195 1101 38-39 0.22831974083865703 1101 40-41 0.23720771961044562 1101 42-43 0.22035838187716195 1101 44-45 0.22512597686508684 1101 46-47 0.3630017412633464 1101 48-49 0.3205530930485878 1101 50-51 0.19262302824314048 1101 52-53 0.18647262743410664 1101 54-55 0.17289949288309714 1101 56-57 0.036828941341468635 1101 58-59 0.1242240971838342 1101 60-61 0.2424190525614165 1101 62-63 0.14174546143023292 1101 64-65 0.13777168834737807 1101 66-67 0.2011612859205485 1101 68-69 0.1133884434570902 1101 70-71 0.10634761617434663 1101 72-73 0.11226820008734961 1101 74-75 0.20736980086184786 1101 76-77 0.18790014281726997 1101 78-79 0.23907220883809543 1101 80-81 0.23369725864635527 1101 82-83 0.10837790975358885 1101 84-85 0.02906052874301679 1101 86-87 0.07502281261542976 1101 88-89 0.05081672313461283 1101 90-91 0.10580673705960564 1101 92-93 0.10574587760346787 1101 94-95 0.13767124438981426 1101 96-97 0.1353547598953213 1101 98-99 0.12180954421691581 1101 100-101 0.1982877222788204 1101 102-103 0.2270712950449223 1101 104-105 0.1349806225365029 1101 106-107 0.16883268131253004 1101 108-109 0.08709096623731938 1101 110-111 0.12703718047007584 1101 112-113 0.23061654935216325 1101 114-115 0.15586132940948616 1101 116-117 0.24102685395548917 1101 118-119 0.23118749754738843 1101 120-121 0.3116770537380127 1101 122-123 0.2647285657952523 1101 124-125 0.2900133553525883 1102 1 0.17051170680423056 1102 2 0.6324548075220449 1102 3 0.35488753728501266 1102 4 0.18084842916601218 1102 5 0.4228880184810464 1102 6 0.22541960295311725 1102 7 0.3671384110093001 1102 8 0.36077510009578617 1102 9 0.3801893134842942 1102 10-11 0.39388956556791754 1102 12-13 0.3304314225585969 1102 14-15 0.32685101392408455 1102 16-17 0.39939089284742124 1102 18-19 0.4007651510585859 1102 20-21 0.31161735104073784 1102 22-23 0.3619327756296613 1102 24-25 0.3258492135772997 1102 26-27 0.359410344472586 1102 28-29 0.3906358048774763 1102 30-31 0.280997671439394 1102 32-33 0.3296128471108446 1102 34-35 0.28178859607378826 1102 36-37 0.3675891983608537 1102 38-39 0.31674347016343063 1102 40-41 0.1992852291721121 1102 42-43 0.21311864724589213 1102 44-45 0.2801566031441354 1102 46-47 0.18601054467768563 1102 48-49 0.1030357479667856 1102 50-51 0.06961258852911811 1102 52-53 0.15425664662326 1102 54-55 0.14195941063583462 1102 56-57 0.1717520822699825 1102 58-59 0.12327940276951921 1102 60-61 0.20022433021399877 1102 62-63 0.16039191434162348 1102 64-65 0.14481820529424994 1102 66-67 0.07997419691109542 1102 68-69 0.0358269353798093 1102 70-71 0.03598129630145763 1102 72-73 0.041680589000620216 1102 74-75 0.031093825978317113 1102 76-77 0.025641174948702883 1102 78-79 0.041964136495579396 1102 80-81 0.024132806206562663 1102 82-83 -0.008464803626907269 1102 84-85 0.05543408936407346 1102 86-87 0.04800343870520152 1102 88-89 -0.014942424055309544 1102 90-91 -0.02845222494759625 1102 92-93 -0.01724659944773066 1102 94-95 0.0019969075887864562 1102 96-97 -0.009833841348093841 1102 98-99 0.01752461266858063 1102 100-101 -0.013255492700796623 1102 102-103 0.04882493449899172 1102 104-105 0.05387018806108301 1102 106-107 -0.027131519869779908 1102 108-109 -0.015283988413369087 1102 110-111 -0.04269463367705484 1102 112-113 -0.026797164231165738 1102 114-115 -0.04109874329106589 1102 116-117 -0.03259655704793829 1102 118-119 0.022771507675834357 1102 120-121 0.06746934088075918 1102 122-123 -0.009374171111328877 1102 124-125 0.09921137435539862 1103 1 0.1796848784528926 1103 2 0.6544194364898317 1103 3 0.35897884511338063 1103 4 0.18222298396364778 1103 5 0.36190941901011797 1103 6 0.2348398144041255 1103 7 0.314493082098835 1103 8 0.3481887388842253 1103 9 0.37613207420800876 1103 10-11 0.3103710237156392 1103 12-13 0.3426395201227095 1103 14-15 0.42435264202423895 1103 16-17 0.518097626642259 1103 18-19 0.46515651209552544 1103 20-21 0.374919032007071 1103 22-23 0.3788791411143535 1103 24-25 0.2577465203469771 1103 26-27 0.3002229119676869 1103 28-29 0.3092838688592394 1103 30-31 0.19397520468447738 1103 32-33 0.2892779070201428 1103 34-35 0.25022759213690193 1103 36-37 0.09287006703703327 1103 38-39 0.14829005916521254 1103 40-41 0.09300307376610562 1103 42-43 0.10584097108408486 1103 44-45 0.10651732508242873 1103 46-47 0.1758783176078822 1103 48-49 0.13299664274734369 1103 50-51 0.1266569550286718 1103 52-53 0.04414349109467253 1103 54-55 0.04858281651863905 1103 56-57 -0.041773595146366915 1103 58-59 0.06809648032460558 1103 60-61 0.13353154432406455 1103 62-63 0.0015604725262576835 1103 64-65 -0.003907716456957644 1103 66-67 -0.018338656485937577 1103 68-69 -0.05815030956657097 1103 70-71 -0.002045798982976521 1103 72-73 0.05680719732730921 1103 74-75 0.03928371758210858 1103 76-77 0.027910756833406936 1103 78-79 -0.015744994766620835 1103 80-81 -0.0171920050810499 1103 82-83 -0.09038323632285739 1103 84-85 -0.05407062579201494 1103 86-87 0.00431111984546817 1103 88-89 0.06412109821525291 1103 90-91 0.014405585584690073 1103 92-93 -0.0036102762765324314 1103 94-95 0.040416304818517546 1103 96-97 -0.01721302125127977 1103 98-99 0.05828986082802601 1103 100-101 0.0493263831034092 1103 102-103 0.04094431344469385 1103 104-105 -0.035218562057899305 1103 106-107 -0.0721236652709365 1103 108-109 -0.0540177065558467 1103 110-111 -0.0011268913571989003 1103 112-113 -0.004353692347823568 1103 114-115 0.06980877445710831 1103 116-117 0.09343003628174529 1103 118-119 0.017823007685080938 1103 120-121 -0.034746672913748 1103 122-123 0.036150488014833115 1103 124-125 -0.0272543437788606 1104 1 0.1137515226492205 1104 2 0.46072786624527495 1104 3 0.24189993415485134 1104 4 0.1550637297589077 1104 5 0.3392071093578508 1104 6 0.21058196412415242 1104 7 0.33475125136333617 1104 8 0.30500679749409443 1104 9 0.33206015812451284 1104 10-11 0.26191351229780935 1104 12-13 0.23471982565646243 1104 14-15 0.22163758084755614 1104 16-17 0.30225784487167573 1104 18-19 0.30548305090125183 1104 20-21 0.30618002656311916 1104 22-23 0.27391024996271085 1104 24-25 0.19564363055141598 1104 26-27 0.14548055090077838 1104 28-29 0.2465609823478161 1104 30-31 0.13494774608764715 1104 32-33 0.07240245672947765 1104 34-35 0.08383890752382683 1104 36-37 0.15732266876441514 1104 38-39 0.15342641719470862 1104 40-41 0.1318842237446134 1104 42-43 0.08089771654621813 1104 44-45 0.07822095192081235 1104 46-47 0.014736182108592288 1104 48-49 -0.006075456005866897 1104 50-51 -0.08053282662405792 1104 52-53 0.027690056943377783 1104 54-55 -0.05562141529040332 1104 56-57 -0.007166376275051789 1104 58-59 0.07841550217347759 1104 60-61 0.03149047545876016 1104 62-63 -0.012242974078013447 1104 64-65 -0.06639819013327752 1104 66-67 -0.08163469377097954 1104 68-69 -0.01569649357664815 1104 70-71 0.0503028719279115 1104 72-73 -0.0330634037699582 1104 74-75 -0.033366236573684205 1104 76-77 0.024508533448390324 1104 78-79 -0.002950258168482378 1104 80-81 -0.035912900689041294 1104 82-83 -0.06125360446714723 1104 84-85 -0.025525586119293564 1104 86-87 -0.01716106643206672 1104 88-89 0.02669423484669764 1104 90-91 -0.01508093967597901 1104 92-93 -0.025073034439898834 1104 94-95 -0.011750432951842527 1104 96-97 -0.07270989661710558 1104 98-99 -0.07169098242250627 1104 100-101 -0.14224449981591292 1104 102-103 -0.11118655820802914 1104 104-105 -0.0663077194123396 1104 106-107 0.027087938144870094 1104 108-109 -0.07148461387512839 1104 110-111 -0.01389314719379442 1104 112-113 -0.06319592778511662 1104 114-115 -0.026734181602378726 1104 116-117 -0.04451221101925995 1104 118-119 -0.015375444689979645 1104 120-121 -0.025265034558923816 1104 122-123 -0.012317188088594833 1104 124-125 0.11399726980359404 1105 1 0.14242403790980518 1105 2 0.5440160810812031 1105 3 0.20180094204604515 1105 4 0.15238468613193135 1105 5 0.35193530799362804 1105 6 0.2137200425708201 1105 7 0.2951745276000324 1105 8 0.16881207224255235 1105 9 0.20215820728570577 1105 10-11 0.08500655291933157 1105 12-13 0.19914188572261082 1105 14-15 0.09742717870552298 1105 16-17 0.3076868222701137 1105 18-19 0.2519268631070517 1105 20-21 0.21817724274217198 1105 22-23 0.18402132938460625 1105 24-25 0.11110540629567822 1105 26-27 0.15054570606879025 1105 28-29 0.11603423210274855 1105 30-31 0.095703517632316 1105 32-33 0.08988040373543527 1105 34-35 0.08298608821673525 1105 36-37 0.030847900148877727 1105 38-39 0.012171415756128567 1105 40-41 -0.01695462225755051 1105 42-43 -0.10833556560129409 1105 44-45 -0.08473055187774037 1105 46-47 -0.12222204328332253 1105 48-49 -0.12661806004071252 1105 50-51 -0.0521289934647271 1105 52-53 -0.13243529712089952 1105 54-55 -0.16899714648540964 1105 56-57 -0.15892883086429777 1105 58-59 -0.06029799701043004 1105 60-61 -0.18164594896504838 1105 62-63 -0.12459590661610065 1105 64-65 -0.019197949894184774 1105 66-67 -0.05769790584859891 1105 68-69 -0.013675991615492933 1105 70-71 -0.046783813651813944 1105 72-73 -0.09534546790160903 1105 74-75 -0.04510068098142028 1105 76-77 -0.10487903512062502 1105 78-79 -0.09414346079149993 1105 80-81 -0.12177562336138337 1105 82-83 -0.13212010279447384 1105 84-85 -0.09113697509079088 1105 86-87 -0.07298355296946113 1105 88-89 -0.1002178667845044 1105 90-91 -0.093839189742976 1105 92-93 -0.10690609319771127 1105 94-95 -0.013498509039212792 1105 96-97 -0.11857568725066159 1105 98-99 -0.10661766293195285 1105 100-101 -0.06910923910152533 1105 102-103 -0.15487478958418777 1105 104-105 -0.08871268845641112 1105 106-107 -0.04191419768944016 1105 108-109 -0.06913484108105905 1105 110-111 -0.06101178526827766 1105 112-113 -0.08063210153558131 1105 114-115 -0.029177041489059974 1105 116-117 -0.055469460325141995 1105 118-119 -0.07456340158722341 1105 120-121 -0.13506418285308897 1105 122-123 -0.10897765120339642 1105 124-125 -0.12484957289670717 1106 1 0.11215032647002587 1106 2 0.5389617599246854 1106 3 0.22036609563904364 1106 4 0.15292831762872083 1106 5 0.26996314106240504 1106 6 0.15526985426147633 1106 7 0.26783997766476375 1106 8 0.16631417490024347 1106 9 0.05029782615574874 1106 10-11 0.10028611340688087 1106 12-13 0.09253026609428616 1106 14-15 0.10446050567989573 1106 16-17 0.07544847867245608 1106 18-19 0.05378314090502556 1106 20-21 0.08538988052473684 1106 22-23 -0.008614065573098628 1106 24-25 0.00952409973238133 1106 26-27 0.12187952892242748 1106 28-29 0.02973927692145395 1106 30-31 -0.027361431393984503 1106 32-33 -0.04322764227467246 1106 34-35 -0.044941033247887674 1106 36-37 -0.012630369588798374 1106 38-39 0.09716273516933427 1106 40-41 -0.00976037748060321 1106 42-43 0.007398456899849748 1106 44-45 0.05123295726296817 1106 46-47 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-1.3437644456627424 2216 32-33 -1.0099043292703485 2216 34-35 -1.317589764246506 2216 36-37 -1.0629695709421512 2216 38-39 -0.821928706941037 2216 40-41 -0.6709149740962772 2216 42-43 -0.4489789178220178 2216 44-45 -0.7314548698394958 2216 46-47 -0.5852139054679242 2216 48-49 -0.5885885092801004 2216 50-51 -0.38465892674843616 2216 52-53 -0.40979335073131296 2216 54-55 -0.6408250858417119 2216 56-57 -1.13666589562974 2216 58-59 -1.1438236993670685 2216 60-61 -0.7489747752778868 2216 62-63 -0.6838663439667059 2216 64-65 -0.6470539672036253 2216 66-67 -0.7174888802226604 2216 68-69 -0.5445104451390606 2216 70-71 -0.5050644599023428 2216 72-73 -0.6260148195064943 2216 74-75 -0.5920051105633419 2216 76-77 -0.38588144521282075 2216 78-79 -0.3925962021334435 2216 80-81 -0.3711326314502621 2216 82-83 -0.3752215173287041 2216 84-85 -0.403825040149556 2216 86-87 -0.2821862151728318 2216 88-89 -0.3433667668614895 2216 90-91 -0.3017193094137447 2216 92-93 -0.29901421188935373 2216 94-95 -0.4202736057062957 2216 96-97 -0.46079611362885586 2216 98-99 -0.5028183685246574 2216 100-101 -0.5937845094752561 2216 102-103 -0.5228941029463599 2216 104-105 -0.2962141232178723 2216 106-107 -0.28670887543208323 2216 108-109 -0.2997288827021478 2216 110-111 -0.420559720982137 2216 112-113 -0.28355710062161066 2216 114-115 -0.32220484443626773 2216 116-117 -0.20616596372917684 2216 118-119 -0.2446496470393349 2216 120-121 -0.28856540382765417 2216 122-123 -0.34025564536417363 2216 124-125 -0.5380218081778203 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 131.0 3 170.0 4 323.0 5 739.0 6 2151.0 7 4599.0 8 7300.0 9 11091.0 10 16314.0 11 19639.0 12 22304.0 13 27017.0 14 33933.0 15 27295.0 16 17328.0 17 15522.0 18 14946.0 19 14667.0 20 15012.0 21 15997.0 22 17809.0 23 21275.0 24 27284.0 25 35636.0 26 46276.0 27 58892.0 28 74710.0 29 93668.0 30 118410.0 31 146590.0 32 183869.0 33 230251.0 34 304282.0 35 431418.0 36 723043.0 37 959063.0 38 313007.0 39 1092.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.37866302061962 17.645307368580102 10.436933113503953 24.539096497296324 2 19.575795586825315 20.19350384871033 35.07450939367577 25.15619117078859 3 18.161602210285537 24.615012246329986 31.85568106594267 25.367704477441812 4 12.454099393271884 16.149611736935952 35.46185330507808 35.93443556471409 5 11.302598184924404 40.260707267215075 35.12346562910441 13.31322891875611 6 33.1817274533543 36.8789897393397 16.143608287382374 13.795674519923622 7 27.307957729642325 33.392432815460346 21.939806856707772 17.35980259818956 8 27.731391620094776 32.14677923037276 19.67213357436974 20.449695575162725 9 25.953127185852242 11.754349128915905 19.308457106285065 42.98406657894678 10-11 24.817674478966843 24.445535254535287 29.448331911771202 21.28845835472667 12-13 26.312017138685334 23.6661721423332 28.674458488453027 21.347352230528443 14-15 22.789835562208978 25.304249081402226 26.080071748392108 25.82584360799669 16-17 22.328013556204066 27.81178233094468 27.07965503526725 22.780549077584002 18-19 22.299378061350055 25.121584287398203 29.862381501349912 22.716656149901834 20-21 22.55576228586204 24.672722019672577 30.224425883401967 22.547089811063415 22-23 22.176603661486784 25.22326996464147 30.1347897498503 22.465336624021447 24-25 22.10526237875498 25.39099538051933 29.967422088978356 22.53632015174734 26-27 21.680101395170503 25.605821093383184 29.92139505701011 22.7926824544362 28-29 22.190124333434575 25.892049770876426 29.72361822063516 22.194207675053843 30-31 21.63657864824368 25.18614979868262 29.949201256435583 23.228070296638116 32-33 21.254866393308944 25.765848608444053 29.74269026336443 23.23659473488257 34-35 22.59518300735434 25.6319843278422 29.561484290524596 22.21134837427886 36-37 22.764728218456558 24.960011625804054 29.109525584787566 23.165734570951823 38-39 22.287939486604298 25.616344025108972 28.971136079394967 23.124580408891767 40-41 22.72663235671103 25.447397528429818 29.311646954356114 22.51432316050304 42-43 22.53527277733729 25.263015308484675 29.094189082343387 23.10752283183465 44-45 22.536174639586058 25.582095146491824 29.394671384877093 22.487058829045026 46-47 23.482942120813874 25.855361502477166 28.054435976384624 22.607260400324332 48-49 22.781043917946924 26.228417442817047 27.810402167052334 23.180136472183687 50-51 23.292690193866406 26.20992083452235 27.995415910845907 22.501973060765337 52-53 23.547332625014004 25.688193257675092 28.183896213617004 22.580577903693904 54-55 22.461989054426837 26.53970854573898 28.33815307023821 22.66014932959598 56-57 22.980861485446592 26.497055095940546 27.784310220330223 22.73777319828264 58-59 22.66786136513669 25.74066925567349 28.131855035850208 23.45961434333961 60-61 22.25820276627316 26.186305225659652 28.417278523706855 23.138213484360325 62-63 22.54649157955407 26.06492931641612 28.630157671172306 22.758421432857503 64-65 22.01103289141894 26.39744365369413 28.48134652793061 23.110176926956317 66-67 22.04582245955844 26.576476326978867 28.3750934813606 23.0026077321021 68-69 22.976624252036267 25.766419179453067 28.551698937380763 22.705257631129896 70-71 22.537558196900598 25.893945967911964 28.17459705520198 23.39389877998546 72-73 22.449154067730095 26.404083736938077 27.911601277537162 23.235160917794662 74-75 22.331712566996323 26.852920327824602 28.022998153722867 22.79236895145621 76-77 22.54982631508435 26.490965152235525 28.213269631182015 22.745938901498114 78-79 22.91971375616573 26.48524024388559 27.959525769248227 22.63552023070045 80-81 23.072270347296232 26.724468568457958 27.300653571388477 22.90260751285733 82-83 22.573932966226764 26.830348479841028 27.789898290088704 22.805820263843508 84-85 22.431811077164035 26.78683734016014 27.939848128849427 22.841503453826398 86-87 22.935501285207504 26.964882179854044 27.132299640615614 22.96731689432283 88-89 22.64440828471714 27.214572890978424 26.939752109854293 23.201266714450142 90-91 22.388448212094648 27.024046239522843 27.295994086330982 23.29151146205153 92-93 22.464925058647395 26.507536572584105 27.855439724690328 23.17209864407817 94-95 22.2065891086037 26.344073220706655 28.085891300692463 23.363446369997188 96-97 22.730866589908626 25.995328640261256 27.72630761618166 23.547497153648457 98-99 22.484678307686252 26.425874393396192 27.288606661418697 23.800840637498858 100-101 22.334083801604972 25.91824624817266 27.61943239925734 24.12823755096502 102-103 21.882337121455112 25.36184129365263 28.143298083897932 24.61252350099432 104-105 21.59985877220393 25.9247379705018 27.62034741198953 24.855055845304737 106-107 22.150998407487535 26.083624607222823 27.027597504533336 24.73777948075631 108-109 21.89120128181072 25.887558069403372 27.382901105880027 24.83833954290588 110-111 22.08224417541848 25.8450835322284 27.536293030141874 24.536379262211245 112-113 21.834655609424843 26.027350782508453 27.73701527510186 24.400978332964847 114-115 21.781687228174498 26.483762528897653 27.07288230288888 24.66166794003897 116-117 21.71477496043295 26.106566433839955 27.454221351166503 24.72443725456059 118-119 21.216692536880323 26.52313581781974 27.163625636520145 25.09654600877979 120-121 21.2671305505155 26.48810004810242 27.387216846186124 24.857552555195955 122-123 21.180217441591488 26.524543141337038 27.049857352147427 25.245382064924048 124-125 20.924721923391992 26.167798383355866 27.345343018722673 25.56213667452947 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 164.0 1 210.0 2 924.5 3 1640.5 4 1251.0 5 1209.0 6 1989.0 7 4044.5 8 5830.5 9 6310.0 10 5293.5 11 3470.5 12 2992.5 13 3073.5 14 3236.0 15 3433.5 16 3733.0 17 4151.0 18 4694.5 19 5297.5 20 6098.5 21 7274.5 22 9094.5 23 11646.0 24 14456.5 25 18593.0 26 24136.0 27 30237.5 28 37271.5 29 45587.0 30 54127.5 31 64226.5 32 86016.5 33 91678.0 34 80126.0 35 79802.0 36 84830.5 37 91989.5 38 93663.0 39 94809.5 40 99802.0 41 104792.0 42 108097.5 43 113196.0 44 119684.5 45 128206.5 46 135397.0 47 137049.5 48 132490.0 49 127143.5 50 131146.0 51 133434.0 52 133436.5 53 140620.5 54 148333.5 55 145683.5 56 140287.5 57 132397.5 58 124740.5 59 114141.5 60 90472.5 61 73647.5 62 65791.0 63 48954.0 64 33895.5 65 28056.5 66 20577.0 67 14052.0 68 10570.5 69 7618.5 70 5518.0 71 3968.0 72 2910.0 73 2546.5 74 2048.0 75 1525.5 76 968.5 77 531.5 78 365.0 79 210.0 80 103.5 81 42.0 82 24.5 83 14.5 84 14.0 85 7.5 86 4.0 87 2.5 88 0.5 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06511141107703254 2 1.7270931320167784E-4 3 4.934551805762224E-5 4 4.934551805762224E-5 5 0.06249609861997857 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.233637951440556E-5 16-17 2.467275902881112E-5 18-19 1.233637951440556E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 1.3570017465846116E-4 36-37 0.0 38-39 0.0 40-41 4.934551805762224E-5 42-43 0.002578303318510762 44-45 0.009536021364635498 46-47 0.010831341213648081 48-49 0.008857520491343192 50-51 0.008376401690281376 52-53 0.006587626660692569 54-55 0.006735663214865436 56-57 0.005045579221391874 58-59 0.00363923195674964 60-61 0.002245221071621812 62-63 0.0015050383007574784 64-65 0.001998493481333701 66-67 0.0026153124570539787 68-69 0.001208965192411745 70-71 0.002701667113654818 72-73 0.0024302667643378954 74-75 7.895282889219559E-4 76-77 5.921462166914668E-4 78-79 1.233637951440556E-4 80-81 3.5775500591776126E-4 82-83 1.603729336872723E-4 84-85 2.960731083457334E-4 86-87 2.714003493169223E-4 88-89 0.0011842924333829337 90-91 0.0017270931320167784 92-93 4.194369034897891E-4 94-95 0.0011472832948397171 96-97 0.0014186836441566395 98-99 0.0014186836441566395 100-101 6.908372528067114E-4 102-103 8.14201047950767E-4 104-105 6.538281142634947E-4 106-107 5.798098371770613E-4 108-109 9.005557045516059E-4 110-111 9.005557045516059E-4 112-113 0.01097937776782095 114-115 0.020256335162653932 116-117 0.01861559668723799 118-119 0.011139750701508222 120-121 0.0059831440644866965 122-123 0.005933798546429074 124-125 0.0052552976731367685 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4053053.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.063761169893663 #Duplication Level Percentage of deduplicated Percentage of total 1 40.67387535063119 8.16070920889089 2 15.125404883017055 6.06945022341595 3 11.368092765592413 6.842600946181264 4 8.112877425086632 6.5109934023024 5 5.863251619064668 5.881944008195291 6 4.16332816994879 5.011921324424579 7 2.883780439696961 4.0501637405943525 8 2.086198343996348 3.3485588261576305 9 1.5736014084298926 2.8415126551811087 >10 7.482908902246704 25.934100236344314 >50 0.4055753909246012 5.620109383879269 >100 0.23028141065862545 8.627080438339704 >500 0.01590910488667335 2.1776182414515097 >1k 0.012677567946532792 5.20397493478862 >5k 0.0018643482274312928 2.7610495573659377 >10k+ 3.7286964548625853E-4 0.9582128724872305 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 15481 0.38195898252502497 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12834 0.3166501893757619 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 10110 0.24944159378128042 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9942 0.24529657026444016 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9720 0.23981921776004408 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 9186 0.22664396443865895 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 8754 0.21598533253821256 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 7539 0.18600793031820703 No Hit CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA 7391 0.182356361981943 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 7237 0.17855675709150606 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 7235 0.17850741157344846 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 7228 0.17833470226024678 No Hit CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT 6800 0.1677747613959156 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 6740 0.16629439585418695 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 6260 0.15445147152035762 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 5948 0.14675357070336856 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 5730 0.14137490923508772 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 5010 0.12361052273434373 No Hit GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG 4948 0.12208081167455742 No Hit TCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCC 4609 0.11371674636379046 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4402 0.10860948524482655 No Hit CCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAA 4368 0.10777061143784697 No Hit GCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTT 4353 0.10740052005241481 No Hit GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA 4226 0.10426707965575581 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.1943690348978903E-4 0.0 0.0 0.0 0.0 7 4.1943690348978903E-4 0.0 0.0 0.0 0.0 8 5.428006986338446E-4 0.0 0.0 0.0 0.0 9 5.428006986338446E-4 0.0 0.0 0.0 0.0 10-11 5.674734576626558E-4 0.0 0.0 0.0 0.0 12-13 5.92146216691467E-4 0.0 0.0 0.0 0.0 14-15 5.92146216691467E-4 0.0 0.0 0.0 0.0 16-17 0.0010856013972676893 0.0 0.0 0.0 0.0 18-19 0.0010856013972676893 0.0 0.0 2.467275902881112E-5 0.0 20-21 0.0010856013972676893 0.0 0.0 3.700913854321668E-5 0.0 22-23 0.0010856013972676893 0.0 0.0 4.934551805762224E-5 0.0 24-25 0.0010856013972676893 0.0 0.0 6.16818975720278E-5 0.0 26-27 0.0010856013972676893 0.0 0.0 1.3570017465846116E-4 0.0 28-29 0.0010856013972676893 0.0 0.0 3.5775500591776126E-4 0.0 30-31 0.0010856013972676893 0.0 0.0 0.0015913929573583173 0.0 32-33 0.0010856013972676893 0.0 0.0 0.005489688883910474 0.0 34-35 0.0010856013972676893 0.0 0.0 0.010880686731705703 0.0 36-37 0.0010856013972676893 0.0 0.0 0.021045863451575886 0.0 38-39 0.0010856013972676893 0.0 0.0 0.03885959547037751 0.0 40-41 0.0010856013972676893 0.0 0.0 0.057758928886446834 0.0 42-43 0.0010856013972676893 0.0 0.0 0.08288813395729096 0.0 44-45 0.001122610535810906 0.0 0.0 0.11549318501386485 0.0 46-47 0.0011596196743541226 0.0 0.0 0.15342755202066194 0.0 48-49 0.0011596196743541226 0.0 0.0 0.19529722409255443 0.0 50-51 0.0013570017465846117 0.0 0.0 0.23996725431421695 0.0 52-53 0.001406347264642234 0.0 0.0 0.28473597557199476 0.0 54-55 0.001431020023671045 0.0 0.0 0.3390777273329513 0.0 56-57 0.001455692782699856 0.0 0.0 0.40816145261362236 0.0 58-59 0.001455692782699856 0.0 0.0 0.4894335208545262 0.0 60-61 0.0016777476139591561 0.0 0.0 0.5718528723902698 0.0 62-63 0.0018257841681320228 0.0 0.0 0.6561720263712317 0.0 64-65 0.0018257841681320228 0.0 0.0 0.7506563570720639 0.0 66-67 0.0018381205476464286 0.0 0.0 0.8747603349869839 0.0 68-69 0.001850456927160834 0.0 0.0 1.019157657203101 0.0 70-71 0.0019491479632760785 0.0 0.0 1.1648996447862883 0.0 72-73 0.002368584866765868 0.0 0.0 1.3168715040242502 0.0 74-75 0.002368584866765868 0.0 0.0 1.4794649860241156 0.0 76-77 0.002442603143852301 0.0 0.0 1.6700373767626528 0.0 78-79 0.002467275902881112 0.0 0.0 1.901369165416786 0.0 80-81 0.002467275902881112 0.0 0.0 2.1426564123390444 0.0 82-83 0.002467275902881112 0.0 0.0 2.395774247215617 0.0 84-85 0.002467275902881112 0.0 0.0 2.669271781049989 0.0 86-87 0.002467275902881112 0.0 0.0 2.9647157340404875 0.0 88-89 0.0025166214209387345 0.0 0.0 3.2910746540940865 0.0 90-91 0.0025166214209387345 0.0 0.0 3.655096540805166 0.0 92-93 0.0025412941799675456 0.0 0.0 4.005091964008366 0.0 94-95 0.0025659669389963566 0.0 0.0 4.38561992650972 0.0 96-97 0.0025659669389963566 0.0 0.0 4.809436244727124 0.0 98-99 0.0025659669389963566 0.0 0.0 5.254212071739501 0.0 100-101 0.0025659669389963566 0.0 0.0 5.749419018206769 0.0 102-103 0.0025906396980251677 0.0 0.0 6.245662220553247 0.0 104-105 0.00275101263171244 0.0 0.0 6.769094803349475 0.0 106-107 0.0028990491858853066 0.0 0.0 7.308848416243261 0.0 108-109 0.002911385565399712 0.0 0.0 7.882021281241572 0.0 110-111 0.002911385565399712 0.0 0.0 8.496397653817997 0.0 112-113 0.002911385565399712 0.0 0.0 9.113673075580309 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8565 0.0 81.24648 1 GTATCAA 13220 0.0 52.372036 2 GTACATG 16500 0.0 44.84631 1 TACATGG 16920 0.0 43.2417 2 ACATGGG 16905 0.0 42.29403 3 TCAACGC 18260 0.0 39.7098 5 ATCAACG 18410 0.0 39.418594 4 CAACGCA 18465 0.0 39.244236 6 AACGCAG 18800 0.0 38.608227 7 TATCAAC 19025 0.0 38.238224 3 CATGGGG 10035 0.0 33.636974 4 ACGCAGA 21965 0.0 32.80128 8 CGCAGAG 22355 0.0 31.989517 9 CATGGGA 6915 0.0 31.251123 4 GAGTACA 10700 0.0 29.733536 1 GTGGTAT 2445 0.0 29.7283 1 AGGTAAG 2275 0.0 26.691315 2 TGGTATC 2680 0.0 26.211895 2 TAAGGTG 2775 0.0 26.17267 5 CAGAGTA 24415 0.0 25.610842 10-11 >>END_MODULE