FastQCFastQC Report
Thu 26 May 2016
SRR936237_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936237_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193027
Sequences flagged as poor quality0
Sequence length125
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54172.80634315406653No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26711.383744243033358No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21091.0925932641547556No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12780.662083542716822No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11310.5859283934371876No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11020.5709045884772597No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT6480.3357043315183886No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG6250.3237888999984458No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG5240.27146461375869696No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5070.2626575556787393No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC4770.24711568847881385No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT4760.24659762623881631No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4570.23675444367886356No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4260.22069451423894068No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT4150.214995829598968No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4130.213959705118973No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC4080.2113693939189854No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG3860.19997202463904015No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT3850.19945396239904262No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT3770.19530946447906253No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA3290.17044247695918188No Hit
ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3260.16888829023918933No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA3250.16837022799919182No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG3230.16733410351919678No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG2820.14609355167929874No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG2800.14505742719930373No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC2780.1440213027193087No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT2670.13832261807933605No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT2640.13676843135934352No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA2640.13676843135934352No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC2540.13158780895936836No Hit
GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA2540.13158780895936836No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA2540.13158780895936836No Hit
GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT2530.13106974671937086No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA2460.12744331103938827No Hit
GTCCATGACCATTAACTGGAGCCTTTTCAGCCCTCCTTCTAACATCAGGT2430.12588912431939575No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT2420.12537106207939822No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA2400.12433493759940319No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT2320.12019043967942308No Hit
CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC2280.11811819071943304No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2260.11708206623943801No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA2250.11656400399944049No Hit
GGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAA2190.11345563055945541No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG2180.11293756831945789No Hit
ACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.10672082143948773No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC2050.10620275919949022No Hit
GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG2040.1056846969594927No Hit
GGTTAATAGTGTAATTGAATTATAGTGAAATCATATTACTAGACCTGATG2030.1051666347194952No Hit
GAGTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCA2000.10361244799950266No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG2000.10361244799950266No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC1940.10050407455951758No Hit
CAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGT1940.10050407455951758No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGTT205.6845784E-6118.990947
GAACAAA6600.082.031631
GGGATAT304.2568223E-579.3272867
TGGGTAT950.075.152166
CATGGGT1600.074.369334
ATGGGTA1350.070.5131455
GAAAACA3650.065.200511
GGGTATG1100.064.9041447
TGGGATA657.4323907E-964.072046
GATGACC1950.061.020999
ATAGGTA300.00423249759.495473
ACCTGGG507.4319614E-659.495473
AGGAAGC150.004235701659.4954712-13
TACCTGG507.4319614E-659.495472
GGATGAC2150.055.344628
ATGGGAT5450.053.4913375
GAGTTAG453.1655031E-452.884869
TGGGAGT453.1655031E-452.884866
GGTAACA350.007788830450.9961131
CTCCCGC350.007788830450.9961137
Produced by FastQC (version 0.11.5)