##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936237_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 193027 Sequences flagged as poor quality 0 Sequence length 125 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.43517746222031 31.0 26.0 33.0 7.0 34.0 2 26.952675014376226 31.0 26.0 34.0 10.0 34.0 3 27.53939604304061 31.0 26.0 34.0 10.0 34.0 4 30.000481797883197 35.0 28.0 37.0 7.0 37.0 5 31.572531303910853 35.0 31.0 37.0 17.0 37.0 6 30.59738793018593 35.0 30.0 37.0 8.0 37.0 7 30.83124122532081 35.0 31.0 37.0 10.0 37.0 8 30.43128681479793 35.0 30.0 37.0 8.0 37.0 9 31.219539235443747 37.0 28.0 39.0 8.0 39.0 10-11 31.54952934045496 37.0 30.5 39.0 8.0 39.0 12-13 31.687191429178302 37.0 30.5 39.0 8.0 39.0 14-15 32.644788034834505 38.0 31.0 41.0 8.0 41.0 16-17 32.73951312510685 38.0 31.0 41.0 8.0 41.0 18-19 32.65622425878245 38.5 31.0 41.0 7.0 41.0 20-21 32.55562175239733 38.5 31.0 41.0 2.0 41.0 22-23 32.147300636698496 38.0 30.0 41.0 2.0 41.0 24-25 31.82059245597766 38.0 30.0 41.0 2.0 41.0 26-27 31.671802390339174 38.0 30.0 41.0 2.0 41.0 28-29 31.442811109326676 38.0 29.0 41.0 2.0 41.0 30-31 31.31723282235128 38.0 29.0 40.0 2.0 41.0 32-33 31.10657576401229 38.0 28.5 40.0 2.0 41.0 34-35 31.02676050500707 38.0 28.0 40.0 2.0 41.0 36-37 30.893007195884515 38.0 27.0 40.0 2.0 41.0 38-39 30.64330378651691 38.0 27.0 40.0 2.0 41.0 40-41 30.705787273283015 38.0 27.0 40.0 2.0 41.0 42-43 30.58935537515477 38.0 26.5 40.0 2.0 41.0 44-45 30.518316090495112 38.0 26.5 40.0 2.0 41.0 46-47 30.532376299688643 38.0 27.0 40.0 2.0 41.0 48-49 30.47213343211053 38.0 26.5 40.0 2.0 41.0 50-51 30.38701321576774 38.0 27.0 40.0 2.0 41.0 52-53 30.321488703652857 38.0 27.0 40.0 2.0 41.0 54-55 30.23941987390365 38.0 27.5 40.0 2.0 41.0 56-57 30.146945764064093 37.5 28.0 40.0 2.0 41.0 58-59 29.957622508768203 37.0 27.0 40.0 2.0 41.0 60-61 29.810959088624905 37.0 27.0 40.0 2.0 41.0 62-63 29.607117139053088 36.5 27.0 40.0 2.0 41.0 64-65 29.378353805426183 36.0 26.0 40.0 2.0 41.0 66-67 29.134626243996955 36.0 26.0 39.0 2.0 41.0 68-69 28.939938454205887 35.5 26.0 39.0 2.0 41.0 70-71 28.64146466556492 35.0 26.0 39.0 2.0 40.5 72-73 28.342527211219156 35.0 26.0 38.5 2.0 40.0 74-75 28.059390655193315 35.0 25.5 37.5 2.0 40.0 76-77 27.750317313121997 34.0 25.0 37.0 2.0 39.0 78-79 27.432680402223525 34.0 25.0 37.0 2.0 39.0 80-81 27.16065369093443 34.0 25.0 36.0 2.0 39.0 82-83 26.842840638874357 34.0 24.5 36.0 2.0 37.5 84-85 26.68585482859911 34.0 24.5 35.5 2.0 37.0 86-87 26.521217239039096 34.0 24.0 35.0 2.0 37.0 88-89 26.30997736068011 34.0 24.0 35.0 2.0 36.0 90-91 26.10830609189388 34.0 23.0 35.0 2.0 36.0 92-93 25.93170385490113 34.0 22.5 35.0 2.0 36.0 94-95 25.77432690763468 34.0 20.5 35.0 2.0 36.0 96-97 25.641845441311318 34.0 20.0 35.0 2.0 35.0 98-99 25.541310283017403 34.0 19.5 35.0 2.0 35.0 100-101 25.433742429815517 34.0 18.5 35.0 2.0 35.0 102-103 24.60229397959871 31.5 17.0 34.5 2.0 35.0 104-105 25.151292306257673 33.0 17.0 35.0 2.0 35.0 106-107 25.21677796370456 34.0 15.0 35.0 2.0 35.0 108-109 25.170657472788783 34.0 10.5 35.0 2.0 35.0 110-111 25.060323167225313 33.5 7.0 35.0 2.0 35.0 112-113 25.0206991767991 34.0 3.5 35.0 2.0 35.0 114-115 24.92230620586757 34.0 2.0 35.0 2.0 35.0 116-117 24.79576950374818 33.5 2.0 35.0 2.0 35.0 118-119 24.62788107363219 33.0 2.0 35.0 2.0 35.0 120-121 24.444601014365865 33.0 2.0 35.0 2.0 35.0 122-123 24.201412237666233 33.0 2.0 35.0 2.0 35.0 124-125 22.542110689178198 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7284871334583478 1101 2 0.60195025727878 1101 3 1.0380965057807394 1101 4 0.6172560945248158 1101 5 0.48877137838704954 1101 6 0.3470367277902753 1101 7 0.2707841549407277 1101 8 0.7480800082132717 1101 9 1.192618850656956 1101 10-11 0.7829321546062289 1101 12-13 0.8340774369432467 1101 14-15 1.3654958609785695 1101 16-17 1.5519883384838806 1101 18-19 1.372049510272717 1101 20-21 1.778289941149012 1101 22-23 1.3875149699986409 1101 24-25 1.3737056681924003 1101 26-27 1.0447387479388084 1101 28-29 1.5605431466114368 1101 30-31 1.498684477107819 1101 32-33 1.3674693350846212 1101 34-35 1.3389792645591108 1101 36-37 1.1754770150261251 1101 38-39 1.0791768344784387 1101 40-41 1.3284173388972427 1101 42-43 1.5212399525160372 1101 44-45 1.4929233173965528 1101 46-47 1.3508243755301095 1101 48-49 1.1773419118281936 1101 50-51 1.2966507021302505 1101 52-53 1.0976036266196019 1101 54-55 1.4320521099479429 1101 56-57 1.3976071916101667 1101 58-59 1.3439664609376365 1101 60-61 1.3421898122168407 1101 62-63 1.1526656307088565 1101 64-65 1.2585022101025736 1101 66-67 1.516733811355845 1101 68-69 1.2901977268995246 1101 70-71 1.513601045394946 1101 72-73 1.5784454998872732 1101 74-75 1.4301447617946437 1101 76-77 1.6050309710175128 1101 78-79 1.505801401159271 1101 80-81 1.5354606610197123 1101 82-83 1.1657007385355662 1101 84-85 1.2063729927867968 1101 86-87 1.2236308161934524 1101 88-89 1.1036971204177277 1101 90-91 1.1358128125252662 1101 92-93 1.1109544718998663 1101 94-95 1.041167135372497 1101 96-97 1.2745575441173358 1101 98-99 1.2489531327148882 1101 100-101 1.2583718381820752 1101 102-103 1.5129820158253509 1101 104-105 1.251334632764614 1101 106-107 1.081753053543693 1101 108-109 0.9748859749648169 1101 110-111 1.135316402938944 1101 112-113 0.9081636917016347 1101 114-115 0.919735106822575 1101 116-117 0.9321269402562713 1101 118-119 1.2216040759958773 1101 120-121 1.3678325769327841 1101 122-123 1.352099773748467 1101 124-125 1.17215514871544 1102 1 0.1954077052969545 1102 2 0.08246027549371604 1102 3 -0.47358865180605747 1102 4 -0.17798594334017181 1102 5 0.06193470564874204 1102 6 -0.5182720981742897 1102 7 0.47992474113049255 1102 8 -0.12145689083182631 1102 9 0.22483168942716958 1102 10-11 0.022535841750901398 1102 12-13 -0.4379598760776773 1102 14-15 -0.4384451888641223 1102 16-17 -0.2552038014951421 1102 18-19 -0.05603460961468443 1102 20-21 0.23875800981380735 1102 22-23 0.2057354259240114 1102 24-25 0.2032856207231788 1102 26-27 0.027578070122398657 1102 28-29 -0.10395980641610691 1102 30-31 -0.24656032059599298 1102 32-33 -0.19543843110183445 1102 34-35 0.0037085243703387505 1102 36-37 -0.06792973001996927 1102 38-39 -0.1225673836592236 1102 40-41 -0.28379770774403923 1102 42-43 -0.05144868246767942 1102 44-45 -0.03289773465401069 1102 46-47 0.006827024975379459 1102 48-49 -0.22954664588003482 1102 50-51 -0.20797478774114353 1102 52-53 -0.4055867470625749 1102 54-55 -0.52388430336552 1102 56-57 -0.8097781370866386 1102 58-59 -0.7280818914385847 1102 60-61 -0.5762183127486615 1102 62-63 -0.6317026421840062 1102 64-65 -0.6023080785765664 1102 66-67 -0.24381153279605527 1102 68-69 -0.48863982898721403 1102 70-71 -0.5557592239653211 1102 72-73 -0.27698310308231555 1102 74-75 -0.1947489844105803 1102 76-77 -0.11318396523020624 1102 78-79 -0.2345143244023582 1102 80-81 -0.3002240549928743 1102 82-83 -0.13353754594862366 1102 84-85 -0.1361616431904622 1102 86-87 -0.27620359347702106 1102 88-89 -0.20905885463333007 1102 90-91 -0.1501120094651327 1102 92-93 -0.07100170185958632 1102 94-95 -0.025080035792708344 1102 96-97 -0.1473279777681853 1102 98-99 -0.11645504744739199 1102 100-101 -0.3278234480132127 1102 102-103 -0.43640254011658186 1102 104-105 -0.3068427696750824 1102 106-107 -0.6189203471297091 1102 108-109 -0.3659872380395406 1102 110-111 -0.19833155202048758 1102 112-113 -0.1594026824221153 1102 114-115 -0.05187167134157633 1102 116-117 -0.2005440317589624 1102 118-119 -0.2637356601635297 1102 120-121 -0.12628344669143132 1102 122-123 -0.16281991494171422 1102 124-125 -0.09139841975616037 1103 1 0.11344049218219965 1103 2 -0.08803152778497392 1103 3 -0.21129356983884406 1103 4 -0.3911006974385316 1103 5 -0.006917753367652324 1103 6 -0.41663275391199406 1103 7 -0.7135178818203265 1103 8 -0.2853913170613325 1103 9 -0.5161519171302054 1103 10-11 -0.3971362893966415 1103 12-13 0.18171225506986843 1103 14-15 -0.013855024929696924 1103 16-17 -0.2666792113312084 1103 18-19 -0.4494772325655063 1103 20-21 -0.3776354328091429 1103 22-23 0.29589936035024067 1103 24-25 0.4377118502313735 1103 26-27 0.43741413569617293 1103 28-29 0.5665319968625795 1103 30-31 0.533767548256467 1103 32-33 0.7963648475866876 1103 34-35 0.8037085243703395 1103 36-37 0.6517424011275708 1103 38-39 0.43808835404569635 1103 40-41 0.23095639061661544 1103 42-43 0.763305415892976 1103 44-45 0.7556268555099237 1103 46-47 0.7019089921884998 1103 48-49 0.43438778034947134 1103 50-51 0.0494022614391838 1103 52-53 0.291134564412836 1103 54-55 0.1269353687656256 1103 56-57 0.18202514160188343 1103 58-59 0.12437712495486153 1103 60-61 0.2746013593824834 1103 62-63 0.08633014470123967 1103 64-65 -0.010504799888042982 1103 66-67 0.13815568031869674 1103 68-69 -0.12798409128229693 1103 70-71 -0.03608709281778388 1103 72-73 -0.10649129980362204 1103 74-75 -0.26851947621386074 1103 76-77 -0.23941347342692865 1103 78-79 0.029420101827152934 1103 80-81 0.017808731892372975 1103 82-83 0.014003437657937212 1103 84-85 -0.013210823518331694 1103 86-87 0.03527181635904242 1103 88-89 0.23356409618634189 1103 90-91 0.26300274463322637 1103 92-93 0.08965403584533149 1103 94-95 0.1552478330597502 1103 96-97 -0.12929519088294228 1103 98-99 -0.29022553925066674 1103 100-101 -0.2901185299804254 1103 102-103 -0.13148450732969508 1103 104-105 -0.06749850738000163 1103 106-107 -0.2549859209002001 1103 108-109 -0.24631510689200198 1103 110-111 -0.1589872897254061 1103 112-113 -0.23481251848768991 1103 114-115 -0.0699044582268229 1103 116-117 0.26011170594595256 1103 118-119 0.08380532344303049 1103 120-121 0.0556837664233214 1103 122-123 0.2125899211238611 1103 124-125 0.4987655146700689 1104 1 -0.593944566823513 1104 2 -0.08715578687612435 1104 3 -0.29522586011491825 1104 4 -0.3597342212277148 1104 5 -0.301070580836015 1104 6 -0.20638017741816128 1104 7 -0.460535556835012 1104 8 -0.4563530300959897 1104 9 -0.33903865819925016 1104 10-11 -0.3450030059022211 1104 12-13 -0.7395031024353429 1104 14-15 -0.6972159476615793 1104 16-17 -0.7798260047311061 1104 18-19 -0.7621754757438026 1104 20-21 -0.4996304133185703 1104 22-23 -0.7743040892145601 1104 24-25 -0.942573299552361 1104 26-27 -1.411018608470009 1104 28-29 -1.059462556456161 1104 30-31 -1.1678450965878788 1104 32-33 -1.014806188884716 1104 34-35 -0.7854408321736877 1104 36-37 -0.7741934134716715 1104 38-39 -0.6754322312055479 1104 40-41 -0.9925337572981583 1104 42-43 -1.0539637676388232 1104 44-45 -0.9585238360049999 1104 46-47 -0.9517781974307695 1104 48-49 -1.0673690413518173 1104 50-51 -1.4623508223435877 1104 52-53 -1.0504951680589976 1104 54-55 -0.8352956345984985 1104 56-57 -1.1221506473659133 1104 58-59 -1.355849286109379 1104 60-61 -1.2901981279886918 1104 62-63 -1.0836223303348014 1104 64-65 -0.9061848340632999 1104 66-67 -0.8235199751520135 1104 68-69 -0.6810785538230135 1104 70-71 -0.7280719275483847 1104 72-73 -0.7208449068949889 1104 74-75 -0.5022355012045132 1104 76-77 -0.7188923007884931 1104 78-79 -0.6142975251285812 1104 80-81 -0.5491640881001523 1104 82-83 -0.5768386619842936 1104 84-85 -0.2528007204585805 1104 86-87 -0.04733618278657303 1104 88-89 -0.1031048844298823 1104 90-91 -0.2758609281387052 1104 92-93 -0.03479729111574059 1104 94-95 -0.27970170504032055 1104 96-97 -0.4118230917746999 1104 98-99 -0.3542614232683974 1104 100-101 -0.24932181934871878 1104 102-103 -0.2148774693631239 1104 104-105 -0.26716743459738623 1104 106-107 -0.21468581944241194 1104 108-109 -0.31650787668962366 1104 110-111 -0.2142871241890134 1104 112-113 -0.06458770938852965 1104 114-115 0.07225926260406013 1104 116-117 -0.1577128254792619 1104 118-119 0.050884941107362636 1104 120-121 -0.09473007455494553 1104 122-123 -0.24501140316726833 1104 124-125 -0.36198100105588793 1105 1 0.7163291116540265 1105 2 0.367938110083351 1105 3 0.7169969788934303 1105 4 0.5892482562275916 1105 5 0.7618450951906723 1105 6 0.4053692491453056 1105 7 0.23180919635161956 1105 8 0.3626609732765509 1105 9 0.8540130715367056 1105 10-11 0.8794384826238115 1105 12-13 0.8635793153197255 1105 14-15 0.7598574836360115 1105 16-17 0.6381175837771167 1105 18-19 0.4315675199514999 1105 20-21 0.42507396574654166 1105 22-23 0.48671165279660755 1105 24-25 0.5735876606788963 1105 26-27 0.37662134670244285 1105 28-29 0.5696156508607828 1105 30-31 0.946355182013253 1105 32-33 1.2285560868466092 1105 34-35 1.173140815762462 1105 36-37 0.803553059500878 1105 38-39 0.8334180678193484 1105 40-41 0.8408135410040494 1105 42-43 1.2320998916717976 1105 44-45 0.8690262545927325 1105 46-47 1.021697089811184 1105 48-49 1.0192172569189708 1105 50-51 0.9744035792400396 1105 52-53 0.7826426557100774 1105 54-55 0.733961882918809 1105 56-57 0.9500447504785932 1105 58-59 0.80029826775176 1105 60-61 0.8254684723443724 1105 62-63 0.8772425900125072 1105 64-65 0.9736710474579056 1105 66-67 1.0780165205485197 1105 68-69 1.0716943653093445 1105 70-71 0.842311515584516 1105 72-73 1.0291367640833613 1105 74-75 1.0940128099070634 1105 76-77 0.9435447259299821 1105 78-79 1.007349573125449 1105 80-81 0.8114358469627447 1105 82-83 0.8141141329296637 1105 84-85 0.7885181312020322 1105 86-87 0.8661979667991879 1105 88-89 0.7928176937826734 1105 90-91 0.6832810641735776 1105 92-93 0.6600087878352134 1105 94-95 0.8816512909691916 1105 96-97 0.6529935656401733 1105 98-99 0.7211022768897557 1105 100-101 0.6795720503790683 1105 102-103 0.8787890681275812 1105 104-105 1.0823565345260668 1105 106-107 0.9270603602657879 1105 108-109 0.9477442468584591 1105 110-111 0.9151890847408843 1105 112-113 0.9284577361114366 1105 114-115 1.1613906764524238 1105 116-117 1.2446249103105096 1105 118-119 1.3351890143396616 1105 120-121 1.3964196264201583 1105 122-123 1.6693396970977155 1105 124-125 1.6523103989671526 1106 1 0.0893194653517746 1106 2 0.3711535441669511 1106 3 0.6076722200509863 1106 4 0.6503415038160156 1106 5 0.935478535705144 1106 6 0.7751441687594536 1106 7 0.7494940838114275 1106 8 0.5395098478746227 1106 9 0.7125339718582495 1106 10-11 0.5900494150996991 1106 12-13 0.6192063249660222 1106 14-15 0.7437803132180036 1106 16-17 0.5722011850632924 1106 18-19 0.753110928311628 1106 20-21 0.7835948660680856 1106 22-23 0.5831746753046403 1106 24-25 -2.87657311102528 1106 26-27 -0.4481374957412889 1106 28-29 -0.24710460637393794 1106 30-31 -0.4378891909770992 1106 32-33 -5.110672208973323 1106 34-35 -0.8075665797359299 1106 36-37 -0.04853697265346213 1106 38-39 0.4025499006167763 1106 40-41 -0.10452407957472687 1106 42-43 0.3928715958518616 1106 44-45 0.2195085697052761 1106 46-47 0.20658454961825612 1106 48-49 0.2925291540250825 1106 50-51 0.30720100689277174 1106 52-53 0.6797487650348373 1106 54-55 0.4108107575168809 1106 56-57 0.44200616526958925 1106 58-59 0.6909735089093161 1106 60-61 0.647011880704504 1106 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2216 108-109 -0.4805272639535758 2216 110-111 -0.6629757974151147 2216 112-113 -0.7513524317320162 2216 114-115 -0.669594616527764 2216 116-117 -0.6680500877558124 2216 118-119 -0.5837345334664406 2216 120-121 -0.4349026794107189 2216 122-123 -0.4321343197639749 2216 124-125 -0.5983332403296515 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12172.0 3 2776.0 4 4001.0 5 4885.0 6 4991.0 7 1907.0 8 1023.0 9 890.0 10 865.0 11 948.0 12 899.0 13 1018.0 14 1002.0 15 1008.0 16 951.0 17 865.0 18 889.0 19 806.0 20 735.0 21 796.0 22 1020.0 23 2238.0 24 2734.0 25 2543.0 26 4236.0 27 2757.0 28 3008.0 29 3263.0 30 3664.0 31 4548.0 32 5642.0 33 7353.0 34 9849.0 35 14152.0 36 23773.0 37 41729.0 38 17043.0 39 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.59583374346594 26.52582281235268 9.173846145875965 19.70449729830542 2 14.907759018168443 33.705129334238215 32.98295057168168 18.40416107591166 3 12.326772938500831 43.27322084475229 26.31030892051371 18.08969729623317 4 7.911846529242022 25.991700642915237 36.08096276686681 30.015490060975925 5 7.258645253205544 52.82061909079134 31.429607563787076 8.491128092216034 6 20.741247293110774 50.63411146684903 16.3848393480671 12.239801891973103 7 21.68982416876483 44.77531524250619 21.894459813703854 11.640400775025126 8 20.59111514052584 45.90389845874886 18.679445667659632 14.825540733065665 9 22.424788755744135 27.013361102044836 17.6186257596245 32.943224382586536 10-11 17.045763620680276 39.95114609803419 27.164427313631172 15.838662967654365 12-13 18.349721793370705 40.19308679839604 25.870885184072286 15.586306224160976 14-15 17.918941882272488 41.59862606335029 21.913046181264313 18.56938587311291 16-17 16.652937732809043 42.14730886992742 24.309020168580947 16.890733228682592 18-19 16.648706896551722 42.12481349469496 24.914518401856764 16.31196120689655 20-21 17.083456375665076 41.40594644161577 25.544365179279154 15.966232003440004 22-23 17.44192376789728 41.2883707546216 24.708679094630362 16.56102638285076 24-25 16.966802055702917 41.57358670424403 25.25281830238727 16.20679293766578 26-27 16.025167789581115 41.73744783693262 25.8319539747545 16.405430398731767 28-29 16.707982665412942 39.15694639799611 26.899953114760045 17.235117821830908 30-31 16.493632982085316 41.508656864878304 25.473252307979237 16.524457845057142 32-33 16.069980753738516 42.157860789692506 25.710725840502114 16.061432616066874 34-35 16.37978598571684 41.49343736157845 25.7723760455279 16.354400607176807 36-37 16.842593336113623 40.73814081559172 25.01353448119549 17.40573136709917 38-39 15.722016003066951 42.219810335940075 26.005631380309858 16.05254228068312 40-41 15.74541055669099 41.64574963281863 27.146035865913394 15.462803944576983 42-43 16.223203091811467 41.16336659310144 25.203340482629283 17.410089832457803 44-45 15.7538770784171 42.25633400768292 25.34276559314702 16.647023320752957 46-47 16.231660311671085 41.076239224137936 26.03873093501326 16.65336952917772 48-49 15.853045703687584 42.34377752219908 25.454861003180916 16.348315770932416 50-51 15.972625165782494 41.01199850795756 25.611841014588858 17.403535311671085 52-53 16.139878254112162 41.09726719336874 25.51249838103872 17.250356171480377 54-55 16.63778432271551 41.65403873601537 25.3548124719919 16.353364469277214 56-57 16.805336096360577 41.53891982903769 24.470923455510942 17.18482061909079 58-59 15.644743079318124 40.45791640007978 26.05367656772516 17.843663952876938 60-61 16.43897713261426 42.10831701428823 24.621035508169882 16.831670344927627 62-63 15.809651106465761 41.594719841263675 26.257288582537452 16.33834046973312 64-65 16.252816992617536 40.721409143893275 25.630099728014503 17.395674135474678 66-67 16.4118212360543 40.939668593009735 24.984263737174622 17.664246433761342 68-69 16.171830883762375 40.86811689556383 25.67749589435675 17.282556326317046 70-71 17.35698817775844 39.66305057349787 25.787717716784268 17.192243531959424 72-73 16.71216980007978 40.80957586244411 24.724261372761326 17.753992964714783 74-75 16.444590653121065 40.24359286524683 25.386603946598147 17.92521253503396 76-77 15.736399571044466 39.790806467489006 27.22520683634932 17.24758712511721 78-79 15.807633129044124 39.85893165204868 25.912696151315618 18.42073906759158 80-81 15.41468292000601 41.51129116652075 25.00841851139996 18.065607402073287 82-83 17.430722126956333 39.34061038093116 25.009713666999957 18.21895382511255 84-85 15.445248592165864 40.02859703564786 25.43374761043792 19.092406761748357 86-87 16.51064358872075 40.189196330047096 25.961134970755385 17.339025110476772 88-89 17.101493573437914 39.216534474451755 26.36859092251343 17.313381029596897 90-91 16.772005988799492 39.5214141026903 26.12095717179462 17.58562273671559 92-93 16.88494355711895 40.04258471612779 25.20061960243904 17.871852124314216 94-95 16.292539385681795 38.39255648173572 26.50147388707279 18.813430245509696 96-97 17.113668036077854 38.94118439389308 26.049464582674965 17.8956829873541 98-99 16.380134333886797 39.591455059279426 26.145891885311084 17.882518721522693 100-101 16.063121030327522 39.488980759068724 26.244391947198825 18.203506263404932 102-103 16.98626872476059 39.57021445241974 25.521884300860243 17.92163252195942 104-105 15.279806865396372 41.90653072643064 25.26499020857294 17.548672199600055 106-107 15.78954185047131 40.12842796196377 26.226709804094256 17.855320383470662 108-109 16.534474451760634 41.16056303004243 24.67064193092158 17.634320587275354 110-111 16.0102784080901 41.8837358697792 24.091573155947906 18.014412566182795 112-113 15.092966268967553 41.063944422282894 26.29994767571376 17.543141633035795 114-115 15.822656934004051 41.44601532428106 25.2291130256389 17.50221471607599 116-117 15.78288878930504 41.66496136520823 24.938414872646362 17.613734972840376 118-119 15.374772049071616 39.87768360411141 25.865177387267906 18.88236695954907 120-121 15.415659670145498 40.882893104243244 25.37838460920764 18.323062616403618 122-123 15.544975573365386 40.8753697669238 25.497469265957612 18.082185393753207 124-125 15.842861361363955 40.876664922523794 24.8542949950007 18.426178721111555 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4303.0 1 5129.5 2 5646.5 3 4180.0 4 2369.0 5 1371.0 6 921.0 7 777.0 8 699.5 9 615.5 10 515.0 11 458.5 12 400.0 13 335.0 14 336.0 15 339.5 16 320.0 17 310.0 18 311.5 19 322.0 20 365.5 21 404.0 22 450.0 23 617.5 24 671.5 25 684.0 26 896.5 27 1141.5 28 1348.5 29 1710.0 30 2766.0 31 3786.0 32 4947.0 33 6924.0 34 8518.0 35 9734.5 36 11784.0 37 12841.0 38 11871.0 39 11578.5 40 10909.0 41 8888.5 42 6785.5 43 5989.0 44 6294.5 45 6311.0 46 4885.0 47 3297.5 48 2968.0 49 2640.5 50 2656.5 51 2160.5 52 1248.5 53 867.0 54 764.5 55 723.0 56 660.0 57 602.5 58 580.5 59 529.5 60 429.5 61 352.0 62 272.5 63 233.0 64 213.0 65 189.5 66 158.0 67 135.0 68 107.5 69 93.5 70 89.0 71 74.0 72 68.0 73 59.0 74 49.0 75 35.0 76 28.5 77 24.5 78 16.5 79 15.5 80 14.0 81 12.5 82 12.0 83 12.0 84 8.0 85 3.5 86 3.0 87 3.0 88 2.5 89 1.5 90 1.0 91 0.5 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0010361244799950266 6 5.180622399975133E-4 7 5.180622399975133E-4 8 0.0010361244799950266 9 0.0020722489599900532 10-11 0.0012951555999937833 12-13 0.0025903111999875665 14-15 0.0025903111999875665 16-17 0.0020722489599900532 18-19 0.0015541867199925397 20-21 0.0020722489599900532 22-23 0.08055867831961332 24-25 0.0015541867199925397 26-27 0.0012951555999937833 28-29 0.0012951555999937833 30-31 5.180622399975133E-4 32-33 0.0012951555999937833 34-35 0.0012951555999937833 36-37 7.770933599962698E-4 38-39 7.770933599962698E-4 40-41 0.0012951555999937833 42-43 5.180622399975133E-4 44-45 7.770933599962698E-4 46-47 0.0015541867199925397 48-49 5.180622399975133E-4 50-51 0.0015541867199925397 52-53 0.0010361244799950266 54-55 0.0012951555999937833 56-57 0.0010361244799950266 58-59 2.5903111999875665E-4 60-61 5.180622399975133E-4 62-63 0.0012951555999937833 64-65 0.0010361244799950266 66-67 7.770933599962698E-4 68-69 0.0 70-71 5.180622399975133E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.5903111999875665E-4 100-101 5.180622399975133E-4 102-103 2.5903111999875665E-4 104-105 5.180622399975133E-4 106-107 2.5903111999875665E-4 108-109 0.0 110-111 5.180622399975133E-4 112-113 0.0 114-115 0.0 116-117 7.770933599962698E-4 118-119 0.0015541867199925397 120-121 0.0012951555999937833 122-123 0.0 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 193027.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.70196397395183 #Duplication Level Percentage of deduplicated Percentage of total 1 72.48069535524426 19.353769161827103 2 7.910053936595397 4.224279504939724 3 4.658336890303054 3.7316023147020885 4 3.2090334096465023 3.427499779823548 5 2.314617205385899 3.090241261585167 6 1.5501920763649064 2.4835903785480786 7 1.0438089325210507 1.9510223958306352 8 0.7644251290209926 1.632932180472162 9 0.6072717395522098 1.459381330072995 >10 4.534166310969694 24.679967051241537 >50 0.5917504171355399 11.071508130986857 >100 0.3162469442396492 14.598993923129925 >500 0.00776066120833495 1.1936154009542705 >1k 0.009700826510418687 4.295254031819383 >5k 0.0019401653020837376 2.80634315406653 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5417 2.80634315406653 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2671 1.383744243033358 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2109 1.0925932641547556 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1278 0.662083542716822 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1131 0.5859283934371876 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1102 0.5709045884772597 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 648 0.3357043315183886 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 625 0.3237888999984458 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 524 0.27146461375869696 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 507 0.2626575556787393 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 477 0.24711568847881385 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 476 0.24659762623881631 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 457 0.23675444367886356 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 426 0.22069451423894068 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 415 0.214995829598968 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 413 0.213959705118973 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 408 0.2113693939189854 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 386 0.19997202463904015 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 385 0.19945396239904262 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 377 0.19530946447906253 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 329 0.17044247695918188 No Hit ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 326 0.16888829023918933 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 325 0.16837022799919182 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 323 0.16733410351919678 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 282 0.14609355167929874 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 280 0.14505742719930373 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 278 0.1440213027193087 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 267 0.13832261807933605 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 264 0.13676843135934352 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 264 0.13676843135934352 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 254 0.13158780895936836 No Hit GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA 254 0.13158780895936836 No Hit GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA 254 0.13158780895936836 No Hit GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 253 0.13106974671937086 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 246 0.12744331103938827 No Hit GTCCATGACCATTAACTGGAGCCTTTTCAGCCCTCCTTCTAACATCAGGT 243 0.12588912431939575 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 242 0.12537106207939822 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 240 0.12433493759940319 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 232 0.12019043967942308 No Hit CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC 228 0.11811819071943304 No Hit AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 226 0.11708206623943801 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 225 0.11656400399944049 No Hit GGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAA 219 0.11345563055945541 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 218 0.11293756831945789 No Hit ACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 206 0.10672082143948773 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 205 0.10620275919949022 No Hit GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG 204 0.1056846969594927 No Hit GGTTAATAGTGTAATTGAATTATAGTGAAATCATATTACTAGACCTGATG 203 0.1051666347194952 No Hit GAGTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCA 200 0.10361244799950266 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 200 0.10361244799950266 No Hit CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC 194 0.10050407455951758 No Hit CAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGT 194 0.10050407455951758 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.180622399975133E-4 0.0 18-19 0.0 0.0 0.0 0.00155418671999254 0.0 20-21 0.0 0.0 0.0 0.00155418671999254 0.0 22-23 0.0 0.0 0.0 0.0020722489599900532 0.0 24-25 0.0 0.0 0.0 0.0041444979199801064 0.0 26-27 0.0 0.0 0.0 0.005180622399975133 0.0 28-29 0.0 0.0 0.0 0.006475777999968916 0.0 30-31 0.0 0.0 0.0 0.014764773839929128 0.0 32-33 0.0 0.0 0.0 0.033674045599838366 0.0 34-35 0.0 0.0 0.0 0.048438819439767494 0.0 36-37 0.0 0.0 0.0 0.07019743351966305 0.0 38-39 0.0 0.0 0.0 0.10464857247949769 0.0 40-41 0.0 0.0 0.0 0.13625036911934602 0.0 42-43 0.0 0.0 0.0 0.1722556947991732 0.0 44-45 0.0 0.0 0.0 0.214995829598968 0.0 46-47 0.0 0.0 0.0 0.26783817807871435 0.0 48-49 0.0 0.0 0.0 0.322493744398452 0.0 50-51 0.0 0.0 0.0 0.3706735327182208 0.0 52-53 0.0 0.0 0.0 0.4274013479979485 0.0 54-55 0.0 0.0 0.0 0.49397234583762895 0.0 56-57 0.0 0.0 0.0 0.5605433436773094 0.0 58-59 0.0 0.0 0.0 0.6504271423168779 0.0 60-61 0.0 0.0 0.0 0.7258051982365161 0.0 62-63 0.0 0.0 0.0 0.8128396545560983 0.0 64-65 0.0 0.0 0.0 0.9177472581555948 0.0 66-67 0.0 0.0 0.0 1.0449315380749844 0.0 68-69 0.0 0.0 0.0 1.1928383075942743 0.0 70-71 0.0 0.0 0.0 1.3531785708735047 0.0 72-73 0.0 0.0 0.0 1.5000492159127998 0.0 74-75 0.0 0.0 0.0 1.6526185455920674 0.0 76-77 0.0 0.0 0.0 1.8621747216710616 0.0 78-79 0.0 0.0 0.0 2.1069591300698867 0.0 80-81 0.0 0.0 0.0 2.3509664451087153 0.0 82-83 0.0 0.0 0.0 2.58124511078761 0.0 84-85 0.0 0.0 0.0 2.83121014158641 0.0 86-87 0.0 0.0 0.0 3.124692400545001 0.0 88-89 0.0 0.0 0.0 3.4430416470234735 0.0 90-91 0.0 0.0 0.0 3.768125702621913 0.0 92-93 0.0 0.0 0.0 4.124811554860201 0.0 94-95 0.0 0.0 0.0 4.52294238629829 0.0 96-97 0.0 0.0 0.0 4.93117543141633 0.0 98-99 0.0 0.0 0.0 5.3298243250944175 0.0 100-101 0.0 0.0 0.0 5.8240557020520445 0.0 102-103 0.0 0.0 0.0 6.319841265729664 0.0 104-105 0.0 0.0 0.0 6.85836696420708 0.0 106-107 0.0 0.0 0.0 7.416579027804401 0.0 108-109 0.0 0.0 0.0 7.925057116361961 0.0 110-111 0.0 0.0 0.0 8.486118522279266 0.0 112-113 0.0 0.0 0.0 9.076450444756432 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGAGTT 20 5.6845784E-6 118.99094 7 GAACAAA 660 0.0 82.03163 1 GGGATAT 30 4.2568223E-5 79.327286 7 TGGGTAT 95 0.0 75.15216 6 CATGGGT 160 0.0 74.36933 4 ATGGGTA 135 0.0 70.513145 5 GAAAACA 365 0.0 65.20051 1 GGGTATG 110 0.0 64.904144 7 TGGGATA 65 7.4323907E-9 64.07204 6 GATGACC 195 0.0 61.02099 9 ATAGGTA 30 0.004232497 59.49547 3 ACCTGGG 50 7.4319614E-6 59.49547 3 AGGAAGC 15 0.0042357016 59.49547 12-13 TACCTGG 50 7.4319614E-6 59.49547 2 GGATGAC 215 0.0 55.34462 8 ATGGGAT 545 0.0 53.491337 5 GAGTTAG 45 3.1655031E-4 52.88486 9 TGGGAGT 45 3.1655031E-4 52.88486 6 GGTAACA 35 0.0077888304 50.996113 1 CTCCCGC 35 0.0077888304 50.996113 7 >>END_MODULE