FastQCFastQC Report
Thu 26 May 2016
SRR936237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193027
Sequences flagged as poor quality0
Sequence length125
%GC34

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62653.2456599335844207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62193.221829070544535No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55502.875245431986199No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19611.0159200526351235No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15710.8138757790360934No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15080.7812378579162501No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10930.5662420283172821No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8030.4160039787180032No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG6850.35487263439829664No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT6780.35124619871831403No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG5720.2963316012785776No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT4840.25074212415879643No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4520.23416413247887602No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT4370.22639319887891332No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4350.2253570743989183No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA4260.22069451423894068No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3790.19634558895905754No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC3670.1901288420790874No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA3650.18909271759909235No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG3640.18857465535909484No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC3570.18494821967911224No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC3420.17717728607914954No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT3250.16837022799919182No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT3190.16526185455920675No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG3050.15800898319924156No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG2940.15231029855926892No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT2940.15231029855926892No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA2920.15127417407927388No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG2870.1486838628792863No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG2800.14505742719930373No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC2740.14194905375931863No Hit
GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA2680.13884068031933355No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT2680.13884068031933355No Hit
GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG2660.13780455583933854No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA2650.13728649359934103No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.13417812015935593No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC2530.13106974671937086No Hit
CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC2320.12019043967942308No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA2280.11811819071943304No Hit
GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.11656400399944049No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA2210.11449175503945043No Hit
TTCTAGAATTGTGAGTAGAAGTAAAATAATAAATGTAATGGTAGCTGTTG2200.11397369279945292No Hit
GGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTC2120.10982919487947283No Hit
CTACTATACAATCAAAGATATCCTAGGTATCCTAATCATATTCTTAATTC2120.10982919487947283No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT2110.10931113263947531No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT2050.10620275919949022No Hit
TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA2010.10413051023950018No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTC701.8189894E-1276.518833
GTGGTAC800.074.412581
GCAGCGT250.002050133771.436081
CACGCAG250.00205224371.417575
GCGTACT250.00205224371.417574
GGTATCA16750.067.171241
TGGTACT807.2759576E-1266.953982
ACTCCCG807.2759576E-1266.936636
TCCCGCT851.4551915E-1162.9991848
ACGTAAC300.004222760459.5300671
CAGCGTA300.00422709859.514642
TGGGGCG300.00422709859.514645
CAGACCC300.00422709859.514644
GGACCTT300.00423143959.499226
CCCGCTG1001.8189894E-1259.499229
GGGGCGC300.00423143959.499226
CTCCCGC902.3646862E-1159.4992187
CTTACTA2150.052.5807089
ATATAGG2050.052.256762
AGCGTAC350.00777891251.012553