FastQCFastQC Report
Thu 26 May 2016
SRR936236_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936236_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5469671
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66650.054.896721
GTACATG302550.052.621161
TACATGG309150.050.6894952
ACATGGG312600.048.7416273
CATGGGA186100.042.071594
GTATCAA86250.041.9387972
CATGGGG136600.040.287514
ATCAACG97650.037.4698754
CAACGCA98900.037.0564546
TCAACGC99800.036.662665
AACGCAG104550.035.28157
TATCAAC116050.031.6827413
ATGGGGG83150.031.4825465
ATGGGAG80150.031.32485
GAGTACA182650.030.8787781
AGTACAT184750.028.8854712
ACGCAGA123700.028.5212388
ATGGGAT93900.028.3218335
CGCAGAG126650.027.6220259
ATGGGGA80000.024.1697835
Produced by FastQC (version 0.11.5)