##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936236_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5469671 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.54816898493529 31.0 30.0 33.0 26.0 34.0 2 29.679028775222495 31.0 30.0 34.0 25.0 34.0 3 29.730128375180154 31.0 30.0 34.0 25.0 34.0 4 33.280490179391045 35.0 35.0 37.0 27.0 37.0 5 33.43504207108618 35.0 35.0 37.0 28.0 37.0 6 33.632209688663174 37.0 35.0 37.0 28.0 37.0 7 33.609127130315514 37.0 35.0 37.0 28.0 37.0 8 33.55783026072318 37.0 35.0 37.0 28.0 37.0 9 35.00005941856466 39.0 35.0 39.0 28.0 39.0 10-11 34.807607075452985 39.0 35.0 39.0 27.0 39.0 12-13 34.79440920669634 39.0 35.0 39.0 27.0 39.0 14-15 35.91860808447163 40.0 36.0 41.0 27.0 41.0 16-17 35.91511930059413 40.0 36.0 41.0 27.0 41.0 18-19 35.9179948848843 39.5 36.0 41.0 27.0 41.0 20-21 35.99306247121628 40.0 36.0 41.0 27.0 41.0 22-23 35.87066041083641 39.5 35.5 41.0 27.0 41.0 24-25 35.815315491553335 39.0 35.0 41.0 27.0 41.0 26-27 35.88444414664063 39.0 35.0 41.0 27.0 41.0 28-29 35.773011484603 39.0 35.0 41.0 27.0 41.0 30-31 35.697654941220414 39.0 35.0 41.0 27.0 41.0 32-33 35.53224755200084 39.0 35.0 41.0 27.0 41.0 34-35 35.5077167346994 39.0 35.0 41.0 27.0 41.0 36-37 35.407657243004195 39.0 35.0 41.0 26.5 41.0 38-39 35.17892438137504 39.0 34.0 40.5 25.5 41.0 40-41 35.27961197300532 39.0 34.5 40.5 26.5 41.0 42-43 35.21324664682757 39.0 35.0 41.0 25.5 41.0 44-45 35.10225258521034 39.0 34.0 41.0 25.5 41.0 46-47 35.0725883695747 39.0 34.0 41.0 26.0 41.0 48-49 34.98486590875393 39.0 34.0 40.0 26.0 41.0 50-51 34.85263702332371 39.0 34.0 40.0 25.0 41.0 52-53 34.70009192509019 38.0 34.0 40.0 24.5 41.0 54-55 34.53298735883749 38.0 33.0 40.0 24.0 41.0 56-57 34.340675609191116 38.0 33.0 40.0 24.0 41.0 58-59 34.10720004914373 38.0 33.0 40.0 23.0 41.0 60-61 33.86594815300591 38.0 33.0 40.0 23.0 41.0 62-63 33.62528002141262 37.0 32.5 40.0 22.0 41.0 64-65 33.34880022948364 37.0 32.0 40.0 22.0 41.0 66-67 33.06345957553937 36.0 32.0 39.5 21.5 41.0 68-69 32.779302356576835 36.0 32.0 39.0 20.5 41.0 70-71 32.4446536181061 35.5 31.0 39.0 20.0 40.5 72-73 32.12649974742539 35.0 31.0 38.5 19.5 40.0 74-75 31.791533805232525 35.0 31.0 37.5 18.5 40.0 76-77 31.46737043233496 35.0 31.0 37.0 18.0 39.0 78-79 31.129795009608436 35.0 31.0 36.5 17.5 39.0 80-81 30.810368301859473 35.0 30.5 36.0 14.0 38.5 82-83 30.503103568752124 34.0 30.0 36.0 12.5 37.0 84-85 30.5182182804048 34.5 31.0 35.0 13.0 37.0 86-87 30.385481960432354 34.0 31.0 35.0 11.5 36.5 88-89 30.203380605524536 34.0 31.0 35.0 8.5 36.0 90-91 30.0210127812075 34.0 30.5 35.0 7.0 36.0 92-93 29.891702627086712 34.0 30.0 35.0 7.0 36.0 94-95 29.760350668257743 34.0 30.0 35.0 3.5 35.5 96-97 29.644783479664497 34.0 30.0 35.0 2.0 35.0 98-99 29.529066007809245 34.0 30.0 35.0 2.0 35.0 100-101 29.418944484960797 34.0 30.0 35.0 2.0 35.0 102-103 28.43499755286927 33.5 28.0 34.5 2.0 35.0 104-105 29.093594477620318 34.0 29.5 35.0 2.0 35.0 106-107 29.206293943456565 34.0 30.0 35.0 2.0 35.0 108-109 29.144635573145074 34.0 30.0 35.0 2.0 35.0 110-111 29.025335527493336 34.0 30.0 35.0 2.0 35.0 112-113 28.970286055596397 34.0 30.0 35.0 2.0 35.0 114-115 28.842569598793055 34.0 29.5 35.0 2.0 35.0 116-117 28.703762621188734 34.0 29.0 35.0 2.0 35.0 118-119 28.485214558608735 34.0 29.0 35.0 2.0 35.0 120-121 28.271320889318574 34.0 29.0 35.0 2.0 35.0 122-123 27.957679812917448 34.0 29.0 35.0 2.0 35.0 124-125 25.951913926815706 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.46876797109225876 1101 2 0.5513831218299075 1101 3 0.534328399598639 1101 4 0.565513272597947 1101 5 0.4838040723797761 1101 6 0.4587623008549855 1101 7 0.456271001913116 1101 8 0.559035542870518 1101 9 0.6706746698367354 1101 10-11 0.7248660186154581 1101 12-13 0.6892149743403522 1101 14-15 0.7374233971562205 1101 16-17 0.734760944982078 1101 18-19 0.7858466969903048 1101 20-21 0.6189547445822541 1101 22-23 0.7461427956115614 1101 24-25 0.8057797439047292 1101 26-27 0.7599333737135936 1101 28-29 0.8478354127267949 1101 30-31 0.84735907968291 1101 32-33 0.8498431627152669 1101 34-35 0.8345942873245349 1101 36-37 0.7182650084740771 1101 38-39 0.8659615209806972 1101 40-41 0.8423026656902053 1101 42-43 0.8783404230087797 1101 44-45 0.8779595362278627 1101 46-47 0.7952825491531286 1101 48-49 0.7797116432668645 1101 50-51 0.8205839228630083 1101 52-53 0.7404340689493054 1101 54-55 0.7621072430875273 1101 56-57 0.8346760558302861 1101 58-59 0.8468728665953975 1101 60-61 0.8947942355816778 1101 62-63 0.8682920828257252 1101 64-65 0.8638541821299413 1101 66-67 0.8880062215747415 1101 68-69 0.7945869331184099 1101 70-71 0.8016237975093787 1101 72-73 0.7641940965420275 1101 74-75 0.7421001714024769 1101 76-77 0.6679793056981289 1101 78-79 0.6817707596329505 1101 80-81 0.7120609464311798 1101 82-83 0.7390761322790844 1101 84-85 0.5501465636787586 1101 86-87 0.6610031506114247 1101 88-89 0.6181805421631594 1101 90-91 0.5707806522777261 1101 92-93 0.580958647248 1101 94-95 0.7238051142338016 1101 96-97 0.6835125903738231 1101 98-99 0.6747272767489889 1101 100-101 0.6400895732666072 1101 102-103 0.7164959524025178 1101 104-105 0.6848103861212529 1101 106-107 0.6596378452413667 1101 108-109 0.6292108753624532 1101 110-111 0.7312741716476729 1101 112-113 0.6791921517470207 1101 114-115 0.6654416789655606 1101 116-117 0.7268864701211726 1101 118-119 0.695712574615797 1101 120-121 0.7753122752646604 1101 122-123 0.773000372721377 1101 124-125 0.6058396952682195 1102 1 0.2603511655775428 1102 2 0.2622979671368455 1102 3 0.32328458895471357 1102 4 0.36405683821590173 1102 5 0.33470176586862266 1102 6 0.2770423818769103 1102 7 0.3694241594811487 1102 8 0.38788261399339063 1102 9 0.46002763791801726 1102 10-11 0.5852298821850042 1102 12-13 0.49767600704315385 1102 14-15 0.6313397697917225 1102 16-17 0.5189204650578745 1102 18-19 0.4505023946609228 1102 20-21 0.48624634999242033 1102 22-23 0.6333633843272963 1102 24-25 0.6835762661694247 1102 26-27 0.4810871601816231 1102 28-29 0.538266903464816 1102 30-31 0.5234786441900852 1102 32-33 0.5971790769888443 1102 34-35 0.5285731007454189 1102 36-37 0.5330586557673556 1102 38-39 0.5564086117056419 1102 40-41 0.40212596112618115 1102 42-43 0.5743930545877305 1102 44-45 0.5456715245319543 1102 46-47 0.4791108349514275 1102 48-49 0.5188052921936546 1102 50-51 0.41646021741689765 1102 52-53 0.4926839382687831 1102 54-55 0.5057656442113796 1102 56-57 0.5293968078966671 1102 58-59 0.5510073123466483 1102 60-61 0.5641534109875934 1102 62-63 0.5558731455850463 1102 64-65 0.6103621944792508 1102 66-67 0.5739501841347732 1102 68-69 0.488086434898932 1102 70-71 0.5173306974407694 1102 72-73 0.45872417756395123 1102 74-75 0.3657177348642051 1102 76-77 0.47863262661681105 1102 78-79 0.38447784741758895 1102 80-81 0.4248441964556804 1102 82-83 0.4017726022715671 1102 84-85 0.3928471991127829 1102 86-87 0.37856391705266645 1102 88-89 0.38972289898631374 1102 90-91 0.3784185610627233 1102 92-93 0.39153135462164457 1102 94-95 0.39400811825222704 1102 96-97 0.34643983399497813 1102 98-99 0.2945017711787301 1102 100-101 0.39296103264130267 1102 102-103 0.43799689983627843 1102 104-105 0.3733860909793023 1102 106-107 0.3234101834425509 1102 108-109 0.36402322630498674 1102 110-111 0.3750567192643892 1102 112-113 0.3820443765828543 1102 114-115 0.4309274837942034 1102 116-117 0.4659793839700441 1102 118-119 0.4898715638019837 1102 120-121 0.47404643025175375 1102 122-123 0.38412491942332494 1102 124-125 0.43579142515158864 1103 1 0.15237301862576302 1103 2 0.24184216830314398 1103 3 0.2521699033109499 1103 4 0.3448783480390176 1103 5 0.25971316953161505 1103 6 0.3504381281247433 1103 7 0.2969920938240449 1103 8 0.3458473783147724 1103 9 0.4146129600589248 1103 10-11 0.5493633940408742 1103 12-13 0.391805323706798 1103 14-15 0.43982391465476667 1103 16-17 0.39431508318495645 1103 18-19 0.44532370483793926 1103 20-21 0.30106247986691415 1103 22-23 0.4885744756414496 1103 24-25 0.536807214726764 1103 26-27 0.3990406750026665 1103 28-29 0.44040242796333473 1103 30-31 0.47933906094969103 1103 32-33 0.472995811049735 1103 34-35 0.4648319273773822 1103 36-37 0.43154410609287197 1103 38-39 0.5251047367781396 1103 40-41 0.4923785462480268 1103 42-43 0.5330199529750743 1103 44-45 0.5635056015634206 1103 46-47 0.5047219532057738 1103 48-49 0.4033110598449454 1103 50-51 0.4683473760395458 1103 52-53 0.42691987207462034 1103 54-55 0.5247432855971255 1103 56-57 0.44905351937327964 1103 58-59 0.42037488137871293 1103 60-61 0.4524264874968864 1103 62-63 0.442251798146593 1103 64-65 0.3537418651703277 1103 66-67 0.44488392546339384 1103 68-69 0.372368748703245 1103 70-71 0.3989950615077902 1103 72-73 0.3798036955355997 1103 74-75 0.37920860712306137 1103 76-77 0.3405185781786848 1103 78-79 0.23019664664577277 1103 80-81 0.2648580754679557 1103 82-83 0.2527780871819978 1103 84-85 0.2807644168289336 1103 86-87 0.27177058116526354 1103 88-89 0.3143822979225277 1103 90-91 0.3516702745642206 1103 92-93 0.3220622733086067 1103 94-95 0.3706797279639922 1103 96-97 0.4773163831656504 1103 98-99 0.3589449859664491 1103 100-101 0.45289898053301414 1103 102-103 0.44250014403079874 1103 104-105 0.46733950914801525 1103 106-107 0.47955809168493957 1103 108-109 0.38640947503997936 1103 110-111 0.3137773922794942 1103 112-113 0.430945672467562 1103 114-115 0.4131467350141662 1103 116-117 0.3981334932685243 1103 118-119 0.33712124738586624 1103 120-121 0.3053962314365961 1103 122-123 0.3185572192764674 1103 124-125 0.3637376046730161 1104 1 0.2225879884506199 1104 2 0.3130670182267643 1104 3 0.31591534914430497 1104 4 0.31716322405682007 1104 5 0.3108593388549892 1104 6 0.29181530072364836 1104 7 0.2084497740731237 1104 8 0.2293735705437001 1104 9 0.3520234767540771 1104 10-11 0.4259919290154528 1104 12-13 0.4807318405198444 1104 14-15 0.5683906450977929 1104 16-17 0.48752524362712535 1104 18-19 0.3657966258372767 1104 20-21 0.45411200564414145 1104 22-23 0.4744389682419481 1104 24-25 0.4729520371445801 1104 26-27 0.3881693336837486 1104 28-29 0.5009227908154514 1104 30-31 0.45916378052199747 1104 32-33 0.5664047991131227 1104 34-35 0.4530785178901482 1104 36-37 0.5145037143690132 1104 38-39 0.6406704028558323 1104 40-41 0.5493560907168415 1104 42-43 0.6662063601368544 1104 44-45 0.6092061969098239 1104 46-47 0.4986194976832934 1104 48-49 0.5455159839402626 1104 50-51 0.5165030697307174 1104 52-53 0.539853349186636 1104 54-55 0.5291061693605315 1104 56-57 0.5001508178298266 1104 58-59 0.5074458549820875 1104 60-61 0.5556223458356939 1104 62-63 0.4823128857785477 1104 64-65 0.5317962821124738 1104 66-67 0.5418626210824229 1104 68-69 0.4850030327949355 1104 70-71 0.490815861949244 1104 72-73 0.5060060683949459 1104 74-75 0.502127143208007 1104 76-77 0.4864853899254449 1104 78-79 0.46465981641862086 1104 80-81 0.48253957411871085 1104 82-83 0.43852196457998716 1104 84-85 0.4951778750744822 1104 86-87 0.48594561211363896 1104 88-89 0.482490208820348 1104 90-91 0.4932847379917398 1104 92-93 0.5195406099884536 1104 94-95 0.5804099569727406 1104 96-97 0.5174954269899459 1104 98-99 0.5312803152068284 1104 100-101 0.47143032384646233 1104 102-103 0.48897409535579683 1104 104-105 0.4657047072834253 1104 106-107 0.5273503057551565 1104 108-109 0.5092086683321817 1104 110-111 0.5004623907096466 1104 112-113 0.4590870673179559 1104 114-115 0.4384925259642003 1104 116-117 0.4712585023086149 1104 118-119 0.4548180638927377 1104 120-121 0.45068934570954156 1104 122-123 0.45275104600100846 1104 124-125 0.4922419245041958 1105 1 0.14112937594384434 1105 2 0.24111811756647583 1105 3 0.24787564313803045 1105 4 0.2549720515775391 1105 5 0.11573255412758243 1105 6 0.17512005325358615 1105 7 0.09471588948604648 1105 8 0.1451288281311207 1105 9 0.21005281285980715 1105 10-11 0.3505628381614585 1105 12-13 0.2606997233349233 1105 14-15 0.22685458131638825 1105 16-17 0.2146384132413246 1105 18-19 0.2267834437558065 1105 20-21 0.10563430225945325 1105 22-23 0.09510372644994192 1105 24-25 0.24712913728868813 1105 26-27 0.14797266443945034 1105 28-29 0.24792282989197645 1105 30-31 0.28877312954158896 1105 32-33 0.3802751458495237 1105 34-35 0.37424918423002396 1105 36-37 0.3054448313569651 1105 38-39 0.4712658361121882 1105 40-41 0.3137101357622427 1105 42-43 0.4873762157052113 1105 44-45 0.49378432590967947 1105 46-47 0.3377936777208177 1105 48-49 0.4009866447775252 1105 50-51 0.3191434867171523 1105 52-53 0.4355981943412388 1105 54-55 0.4224896092593724 1105 56-57 0.442837206890097 1105 58-59 0.4458196307503286 1105 60-61 0.46007619286677226 1105 62-63 0.4914549459728832 1105 64-65 0.4736253666929571 1105 66-67 0.5080430818079904 1105 68-69 0.45202128631866856 1105 70-71 0.40045816816621027 1105 72-73 0.3881523081828817 1105 74-75 0.42720360161981574 1105 76-77 0.37929452447603396 1105 78-79 0.3816080777656339 1105 80-81 0.42452715788403594 1105 82-83 0.4116339236192772 1105 84-85 0.334131631591557 1105 86-87 0.32645101251647546 1105 88-89 0.32511758434764815 1105 90-91 0.3263051888391786 1105 92-93 0.3792460899266423 1105 94-95 0.3523204323799156 1105 96-97 0.36388911530651313 1105 98-99 0.3428110146049157 1105 100-101 0.4001504027225664 1105 102-103 0.4193382859439865 1105 104-105 0.37145899194503684 1105 106-107 0.4339725146742275 1105 108-109 0.35357595952329746 1105 110-111 0.41784610281504797 1105 112-113 0.38576918560606543 1105 114-115 0.3350405134033565 1105 116-117 0.3279219808267584 1105 118-119 0.4392264492372888 1105 120-121 0.4990638950335615 1105 122-123 0.4712649204281867 1105 124-125 0.4847808196060477 1106 1 -0.38072057531980974 1106 2 -0.4674088396576046 1106 3 -0.30200798694783515 1106 4 -0.28497039513491984 1106 5 -0.26198653212411926 1106 6 -0.1896322582868848 1106 7 -0.3346446389385562 1106 8 -0.32611897556184744 1106 9 -0.31872324945505426 1106 10-11 -0.2586471150474239 1106 12-13 -0.3461238273138747 1106 14-15 -0.4641653641115866 1106 16-17 -0.3743391648689709 1106 18-19 -0.425806480612998 1106 20-21 -0.398142764383536 1106 22-23 -0.47754860369702357 1106 24-25 -4.222187607715572 1106 26-27 -1.3474967981710009 1106 28-29 -0.9511350971113401 1106 30-31 -0.8795459476183041 1106 32-33 -5.802131086905106 1106 34-35 -1.42727237288031 1106 36-37 -0.695799359397725 1106 38-39 -0.31879426235425257 1106 40-41 -0.8793735321709306 1106 42-43 -0.5390392658542567 1106 44-45 -0.47372304268719745 1106 46-47 -0.58444629644152 1106 48-49 -0.36523819680292746 1106 50-51 -0.3156106669725389 1106 52-53 -0.3038041220622887 1106 54-55 -0.49198216436295183 1106 56-57 -0.3624935025038596 1106 58-59 -0.2055190593520706 1106 60-61 -0.18861995135898013 1106 62-63 -0.24736795239122955 1106 64-65 -0.17769035601099148 1106 66-67 -0.24348052360311812 1106 68-69 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-0.9391161992230295 2216 92-93 -0.9076921439991175 2216 94-95 -0.8928450968618975 2216 96-97 -1.0058194278598904 2216 98-99 -0.8820447190324643 2216 100-101 -0.925073962803399 2216 102-103 -0.8801850632528208 2216 104-105 -0.8006208339667928 2216 106-107 -0.9652166828000723 2216 108-109 -0.9706894557007537 2216 110-111 -0.8495922461326906 2216 112-113 -0.9078911942056145 2216 114-115 -1.0081480516813102 2216 116-117 -0.834412167196021 2216 118-119 -0.9490050188461296 2216 120-121 -0.8603633285605738 2216 122-123 -0.796628680346327 2216 124-125 -0.8931987946151345 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 146568.0 3 24884.0 4 17027.0 5 13906.0 6 14468.0 7 15434.0 8 16913.0 9 19341.0 10 21760.0 11 24672.0 12 26146.0 13 26380.0 14 26464.0 15 25523.0 16 23884.0 17 22260.0 18 20545.0 19 19878.0 20 19574.0 21 21081.0 22 24924.0 23 33564.0 24 47603.0 25 66259.0 26 78125.0 27 81911.0 28 96302.0 29 117144.0 30 139385.0 31 171296.0 32 217216.0 33 284708.0 34 389225.0 35 554567.0 36 909743.0 37 1267990.0 38 441598.0 39 1403.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.53228537057148 18.541902056069297 9.4522877107535 23.473524862605718 2 18.512363362389937 20.54546627958657 38.02767589775542 22.91449446026807 3 17.354468798751807 27.56492211868762 30.461313106880873 24.619295975679698 4 11.007155837840012 16.554228233564793 37.834508909146095 34.6041070194491 5 11.12498363696065 41.78994099067508 35.561400339766166 11.5236750325981 6 31.171481450265436 37.938353242905805 18.363817628450246 12.526347678378514 7 27.368530364025318 34.1494615648871 22.375191671418648 16.106816399668936 8 26.172185164092305 35.11358618993772 20.167031255284858 18.54719739068512 9 26.7061733505216 13.803071985034435 19.35345452511078 40.137300139333185 10-11 23.933848493480177 26.70874571680038 29.709462767655413 19.647943022064034 12-13 24.71184664144916 25.598408370170944 28.985302160746823 20.70444282763307 14-15 22.27738987041169 27.311404756900902 25.58420049910501 24.8270048735824 16-17 22.527370615479477 29.60511262483208 26.07481629132674 21.7927004683617 18-19 21.785351925675727 28.7795203286822 27.552721181870808 21.88240656377126 20-21 22.73768194598364 28.139519215677026 27.286026440587296 21.836772397752043 22-23 22.502285982703572 28.33730143698968 27.158883529158377 22.00152905114837 24-25 22.30098586751972 28.18660015112625 27.323264466005643 22.189149515348383 26-27 22.112050990041897 28.35235995500233 27.134870686558127 22.40071836839764 28-29 22.14221881268708 28.41625609639266 27.202197297512736 22.239327793407522 30-31 22.316785652287624 27.99072552966709 27.39721281397299 22.2952760040723 32-33 21.8340587801615 28.422353117014126 27.399722192639615 22.343865910184764 34-35 22.370222022859092 28.42084016688851 27.311443468989133 21.89749434126326 36-37 22.513294709339178 28.115017084252266 27.048250009095636 22.32343819731292 38-39 22.106299500515572 28.30613166497247 27.28307530294498 22.304493531566976 40-41 22.239026113728848 28.134376486902912 27.508731162468408 22.11786623689983 42-43 22.02039075733573 28.047941041769697 27.49740156335843 22.434266637536144 44-45 22.058275719053725 28.260936535727737 27.507815190278183 22.17297255494035 46-47 22.26433463385025 28.244923778982816 27.050041867625175 22.440699719541758 48-49 22.17689947821254 28.19822438689059 27.18297846666886 22.441897668228005 50-51 22.117456896551722 28.135594862689118 27.359134947287128 22.38781329347203 52-53 22.28463048267355 27.957767149999306 27.496136163543834 22.261466203783307 54-55 21.916630481980025 28.161711269054095 27.53706149873163 22.384596750234248 56-57 22.191663586781594 28.37473207742278 27.21305397137887 22.220550364416756 58-59 22.39896424779072 28.04768241027576 27.209803994699477 22.343549347234045 60-61 22.297064624802193 28.00700009315053 27.511620277124194 22.184315004923082 62-63 22.02373835158868 28.138841201324404 27.65004214179021 22.1873783052967 64-65 21.901435140942628 28.11843820714245 27.56928841895724 22.41083823295769 66-67 21.985187184092307 28.505643545557852 27.22289365390563 22.286275616444218 68-69 22.20157399065098 28.052143605164776 27.50307879972152 22.243203604462725 70-71 22.002929255606713 27.954499456912135 27.522305361824863 22.52026592565629 72-73 21.920255591638423 28.234328900549166 27.379367556956215 22.4660479508562 74-75 21.82125264310377 28.365710583211 27.44519607576963 22.3678406979156 76-77 21.831187282424736 28.224629725545963 27.432103171331157 22.512079820698148 78-79 22.053496551248788 28.17956176808764 27.377455385260625 22.389486295402953 80-81 21.975860101686422 28.249039679533922 27.281191321176635 22.49390889760302 82-83 22.048128715720487 28.293893996452436 27.34847063787807 22.30950664994901 84-85 21.836620389876117 28.194921787193188 27.50410194563264 22.46435587729805 86-87 21.79089461270283 28.21961791795948 27.534575713348335 22.454911755989354 88-89 21.895467167563172 28.112334963446994 27.441479675288743 22.550718193701087 90-91 21.98968309759373 28.0279870887888 27.5961781937285 22.38615161988897 92-93 21.932045666105115 28.074769201770096 27.535896436678815 22.457288695445975 94-95 21.70523637283753 27.961726716668515 27.62561831951652 22.707418590977436 96-97 21.82233763460499 28.013150356745108 27.62002730881975 22.544484699830143 98-99 21.570963329916303 28.023234703856524 27.49972895953553 22.90607300669164 100-101 21.636721627735696 27.893354238961308 27.664802534783018 22.80512159851998 102-103 21.552549775831075 27.81931559574909 27.70361344126838 22.924521187151456 104-105 21.463202733479363 27.877907928081076 27.68008023900576 22.978809099433803 106-107 21.48038844834751 27.836890745839916 27.71374778573948 22.968973020073097 108-109 21.530827243897942 27.980809742779726 27.43385523444322 23.054507778879106 110-111 21.429983115906058 28.119484902585086 27.49822886128807 22.952303120220783 112-113 21.56509853808151 28.041010577984526 27.506151202761707 22.887739681172256 114-115 21.43344376067251 28.145935944830207 27.422225513103193 22.99839478139409 116-117 21.200503395506352 28.03210366722365 27.64708855978224 23.12030437748776 118-119 21.266723452864074 28.028092896075957 27.43156358216694 23.27362006889303 120-121 21.286376588810487 27.963585657457923 27.565754568201523 23.184283185530063 122-123 21.33345448655108 27.865425955333954 27.4978984060795 23.303221152035466 124-125 21.332752676146775 27.751875823462296 27.18157576209656 23.73379573829437 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1943.0 1 2064.0 2 2130.0 3 1864.0 4 1514.0 5 1281.0 6 1140.0 7 1117.5 8 1140.0 9 1097.0 10 1038.0 11 1056.5 12 1081.5 13 1136.5 14 1288.0 15 1552.0 16 1944.0 17 2286.5 18 2770.0 19 3469.5 20 4659.0 21 6892.0 22 10014.0 23 14748.5 24 20416.0 25 27260.0 26 36088.5 27 45093.5 28 53520.0 29 62678.0 30 76447.0 31 91842.5 32 104973.5 33 114050.0 34 124327.5 35 134655.0 36 145460.5 37 158643.5 38 164603.5 39 167058.5 40 170495.5 41 175299.0 42 180117.0 43 188366.0 44 200232.0 45 208191.5 46 208151.5 47 207268.0 48 209019.0 49 205029.0 50 198242.0 51 190433.5 52 181577.0 53 177353.5 54 169644.5 55 154522.0 56 140121.0 57 125091.5 58 110772.5 59 98126.0 60 82530.5 61 66753.5 62 55016.0 63 43588.0 64 32068.5 65 24013.0 66 18376.0 67 14364.5 68 10908.5 69 7687.5 70 5429.0 71 3840.5 72 2868.0 73 2422.0 74 1930.5 75 1424.0 76 954.0 77 632.0 78 424.0 79 262.0 80 194.0 81 144.5 82 88.5 83 57.5 84 50.5 85 37.0 86 32.0 87 29.0 88 24.0 89 18.0 90 16.0 91 14.0 92 8.0 93 6.0 94 6.5 95 6.0 96 6.0 97 6.5 98 3.0 99 1.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.011089880908742E-4 2 3.6565270561977126E-5 3 3.6565270561977126E-5 4 2.011089880908742E-4 5 4.936311525866912E-4 6 4.3878324674372554E-4 7 4.3878324674372554E-4 8 6.581748701155883E-4 9 8.775664934874511E-4 10-11 8.227185876444854E-4 12-13 0.0010512515286568425 14-15 0.0014717521401195794 16-17 0.0013437736931526595 18-19 0.0013072084225906823 20-21 0.0015997305870864992 22-23 0.08856108530110861 24-25 0.0013163497402311766 26-27 9.872623051733825E-4 28-29 7.861533170825083E-4 30-31 5.941856466321283E-4 32-33 9.781209875328882E-4 34-35 5.759030113511398E-4 36-37 2.5595689393383993E-4 38-39 5.667616937106455E-4 40-41 7.038814583180597E-4 42-43 6.581748701155883E-4 44-45 6.307509171941055E-4 46-47 9.324143993304167E-4 48-49 2.011089880908742E-4 50-51 7.13022775958554E-4 52-53 7.77011999442014E-4 54-55 8.410012229254739E-4 56-57 7.404467288800368E-4 58-59 4.2050061146273697E-4 60-61 5.393377407891626E-4 62-63 7.495880465205311E-4 64-65 6.216095995536111E-4 66-67 2.8338084685532275E-4 68-69 1.2797844696691996E-4 70-71 4.022179761817484E-4 72-73 1.554023998884028E-4 74-75 2.2853294101235703E-4 76-77 2.1939162337186277E-4 78-79 1.8282635280988564E-4 80-81 2.2853294101235703E-4 82-83 9.141317640494282E-5 84-85 1.9196767045037992E-4 86-87 1.462610822479085E-4 88-89 2.1025030573136849E-4 90-91 1.0969581168593138E-4 92-93 2.2853294101235703E-4 94-95 1.2797844696691996E-4 96-97 1.7368503516939135E-4 98-99 1.462610822479085E-4 100-101 2.1025030573136849E-4 102-103 4.2964192910323123E-4 104-105 2.1939162337186277E-4 106-107 2.1939162337186277E-4 108-109 3.7479402326026553E-4 110-111 4.753485173057026E-4 112-113 1.6454371752889707E-4 114-115 2.011089880908742E-4 116-117 2.8338084685532275E-4 118-119 5.85044328991634E-4 120-121 2.376742586528513E-4 122-123 2.376742586528513E-4 124-125 1.1883712932642565E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 5469671.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.93221247752983 #Duplication Level Percentage of deduplicated Percentage of total 1 48.29005708292364 13.005580779093437 2 13.98662613172398 7.533815736467228 3 10.147669149451248 8.198975450540862 4 7.226804822935712 7.785353720997677 5 5.147562627033434 6.9317625206328035 6 3.5616712631038183 5.755421233981445 7 2.477085536058054 4.669937578749198 8 1.745365023965767 3.7605233341035986 9 1.2961180965027892 3.1416595173886166 >10 5.716138434788016 25.81997741080012 >50 0.2610738686195463 4.786959564759321 >100 0.13525948719000896 6.556495057579546 >500 0.006237855450171564 1.104308292921657 >1k 0.0023306202538306148 0.9492298019844111 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.8282635280988563E-5 0.0 12-13 0.0 0.0 0.0 1.8282635280988563E-5 0.0 14-15 0.0 0.0 0.0 4.570658820247141E-5 0.0 16-17 0.0 0.0 0.0 6.398922348345997E-5 0.0 18-19 0.0 0.0 0.0 1.005544940454371E-4 0.0 20-21 0.0 0.0 0.0 1.462610822479085E-4 0.0 22-23 0.0 0.0 0.0 2.1939162337186277E-4 0.0 24-25 0.0 0.0 0.0 3.108047997768056E-4 0.0 26-27 0.0 0.0 0.0 3.747940232602656E-4 0.0 28-29 0.0 0.0 0.0 5.484790584296569E-4 0.0 30-31 0.0 0.0 0.0 0.0018008395751773737 0.0 32-33 0.0 0.0 0.0 0.005713323525308926 0.0 34-35 0.0 0.0 0.0 0.011380940462415381 0.0 36-37 0.0 0.0 0.0 0.020823921585045972 0.0 38-39 0.0 0.0 0.0 0.03854893648996439 0.0 40-41 0.0 0.0 0.0 0.05764514904095694 0.0 42-43 0.0 0.0 0.0 0.08052586709511413 0.0 44-45 0.0 0.0 0.0 0.11034484523840649 0.0 46-47 0.0 0.0 0.0 0.14765056252926365 0.0 48-49 0.0 0.0 0.0 0.18931668833463658 0.0 50-51 0.0 0.0 0.0 0.2300686823759601 0.0 52-53 0.0 0.0 0.0 0.27602208615472484 0.0 54-55 0.0 0.0 0.0 0.33187553693814487 0.0 56-57 0.0 9.141317640494281E-6 0.0 0.4024556504404012 0.0 58-59 0.0 1.8282635280988563E-5 0.0 0.48408761697001523 0.0 60-61 0.0 1.8282635280988563E-5 0.0 0.5664143236403067 0.0 62-63 0.0 1.8282635280988563E-5 0.0 0.6517028172261183 0.0 64-65 0.0 1.8282635280988563E-5 0.0 0.7493138069913163 0.0 66-67 0.0 1.8282635280988563E-5 0.0 0.8693850141992087 0.0 68-69 0.0 1.8282635280988563E-5 0.0 1.0167887611521789 0.0 70-71 0.0 1.8282635280988563E-5 0.0 1.1671908602912313 0.0 72-73 0.0 1.8282635280988563E-5 0.0 1.3208655511455807 0.0 74-75 0.0 1.8282635280988563E-5 0.0 1.4872649561554983 0.0 76-77 0.0 1.8282635280988563E-5 0.0 1.6769015906075522 0.0 78-79 0.0 1.8282635280988563E-5 0.0 1.9084877317118343 0.0 80-81 0.0 1.8282635280988563E-5 0.0 2.1539778169473083 0.0 82-83 0.0 1.8282635280988563E-5 0.0 2.4105563204807017 0.0 84-85 0.0 1.8282635280988563E-5 0.0 2.6788265692762874 0.0 86-87 0.0 1.8282635280988563E-5 0.0 2.9702609169728857 0.0 88-89 0.0 1.8282635280988563E-5 0.0 3.3058478288730715 0.0 90-91 0.0 1.8282635280988563E-5 0.0 3.6682827906833886 0.0 92-93 0.0 1.8282635280988563E-5 0.0 4.031229666281574 0.0 94-95 0.0 1.8282635280988563E-5 0.0 4.421362820542589 0.0 96-97 0.0 1.8282635280988563E-5 0.0 4.830573904719315 0.0 98-99 0.0 1.8282635280988563E-5 0.0 5.286268589097955 0.0 100-101 0.0 3.6565270561977126E-5 0.0 5.779214142861609 0.0 102-103 0.0 3.6565270561977126E-5 0.0 6.281099905277666 0.0 104-105 0.0 3.6565270561977126E-5 0.0 6.803480501843712 0.0 106-107 0.0 3.6565270561977126E-5 0.0 7.341620730021971 0.0 108-109 0.0 3.6565270561977126E-5 0.0 7.920458104335709 0.0 110-111 0.0 3.6565270561977126E-5 0.0 8.526024691430251 0.0 112-113 0.0 5.484790584296569E-5 0.0 9.150778904252194 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6665 0.0 54.89672 1 GTACATG 30255 0.0 52.62116 1 TACATGG 30915 0.0 50.689495 2 ACATGGG 31260 0.0 48.741627 3 CATGGGA 18610 0.0 42.07159 4 GTATCAA 8625 0.0 41.938797 2 CATGGGG 13660 0.0 40.28751 4 ATCAACG 9765 0.0 37.469875 4 CAACGCA 9890 0.0 37.056454 6 TCAACGC 9980 0.0 36.66266 5 AACGCAG 10455 0.0 35.2815 7 TATCAAC 11605 0.0 31.682741 3 ATGGGGG 8315 0.0 31.482546 5 ATGGGAG 8015 0.0 31.3248 5 GAGTACA 18265 0.0 30.878778 1 AGTACAT 18475 0.0 28.885471 2 ACGCAGA 12370 0.0 28.521238 8 ATGGGAT 9390 0.0 28.321833 5 CGCAGAG 12665 0.0 27.622025 9 ATGGGGA 8000 0.0 24.169783 5 >>END_MODULE