FastQCFastQC Report
Thu 26 May 2016
SRR936236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936236_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5469671
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147120.26897413025390376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114520.20937273923788105No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112570.20580762535808828No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107750.076.248931
GTATCAA161150.050.6121942
GTACATG270150.045.4086071
TACATGG280250.043.187362
ACATGGG279850.042.4828573
ATCAACG220450.038.4287034
TCAACGC223250.038.0000655
CAACGCA224150.037.9556356
AACGCAG230700.036.9811557
CATGGGA170300.036.9156234
TATCAAC238350.035.8674243
CATGGGG119950.032.6081434
ACGCAGA270550.031.0503238
CGCAGAG274600.030.3540449
GAGTACA181300.028.5238881
ATGGGAG71150.028.2815195
AGTACAT182800.027.194132
ATGGGAT90200.025.8726225
CAGAGTA310400.025.5881299
GTGGTAT38500.025.533481
Produced by FastQC (version 0.11.5)