FastQCFastQC Report
Thu 26 May 2016
SRR936235_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936235_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4012561
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75750.1887821767693999No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59200.062.8106231
GTACATG350750.055.2452431
TACATGG350050.053.6561242
ACATGGG354650.051.4168323
CATGGGA248050.048.2813454
ATGGGAG90850.043.6138155
GTATCAA86100.043.2559622
CATGGGG106050.041.458024
ATGGGAT97000.041.400625
TACCTGG35650.037.215182
TCAACGC101850.036.450075
ATCAACG104200.035.6851164
CAACGCA106500.035.082046
AACGCAG108750.034.466057
GAGTACA197850.032.5662081
TATCAAC118550.032.419463
AGTACAT196900.031.031272
ATGGGGA57500.030.730065
ATGGGAC45850.030.3634665
ATGGGGG56250.030.0379755
Produced by FastQC (version 0.11.5)