##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936235_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4012561 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.410118375770487 31.0 30.0 33.0 24.0 34.0 2 29.290106742302484 31.0 30.0 34.0 23.0 34.0 3 29.534234121300585 31.0 30.0 34.0 24.0 34.0 4 32.91092995221755 35.0 35.0 37.0 25.0 37.0 5 33.1795618808038 35.0 35.0 37.0 27.0 37.0 6 33.0611140864899 37.0 35.0 37.0 26.0 37.0 7 32.892644373506094 36.0 33.0 37.0 26.0 37.0 8 32.83198361345784 36.0 35.0 37.0 25.0 37.0 9 34.1060499765611 38.0 35.0 39.0 23.0 39.0 10-11 34.04105221079505 38.0 34.5 39.0 23.0 39.0 12-13 34.057789651048296 38.0 35.0 39.0 23.5 39.0 14-15 35.13028350223212 39.0 35.0 41.0 24.0 41.0 16-17 35.15027086192584 39.0 35.0 41.0 24.5 41.0 18-19 35.16003108737786 39.0 35.0 41.0 24.5 41.0 20-21 35.285906806151985 39.0 35.0 41.0 25.0 41.0 22-23 35.16325110073093 39.0 34.0 41.0 24.5 41.0 24-25 35.13575669005405 39.0 34.0 41.0 25.0 41.0 26-27 35.24341025594377 39.0 34.0 41.0 26.0 41.0 28-29 35.13987176768154 39.0 34.0 41.0 25.5 41.0 30-31 35.067145895102904 39.0 34.0 41.0 25.0 41.0 32-33 34.907932988433075 39.0 34.0 41.0 25.0 41.0 34-35 34.868882242537865 39.0 34.0 41.0 25.0 41.0 36-37 34.74974249612654 39.0 34.0 40.0 24.5 41.0 38-39 34.50568925930347 38.0 33.0 40.0 24.0 41.0 40-41 34.606664546657356 38.5 33.5 40.0 24.0 41.0 42-43 34.50847588360651 38.5 33.0 40.5 24.0 41.0 44-45 34.364239696293716 38.0 33.0 40.0 23.5 41.0 46-47 34.33110188231406 38.0 33.0 40.0 23.5 41.0 48-49 34.22853048215342 38.0 33.0 40.0 23.0 41.0 50-51 34.08113745809721 38.0 33.0 40.0 23.0 41.0 52-53 33.92537185104476 38.0 33.0 40.0 22.0 41.0 54-55 33.758994193483915 38.0 33.0 40.0 22.0 41.0 56-57 33.55961292551066 38.0 32.0 40.0 20.5 41.0 58-59 33.32277814592725 37.5 32.0 40.0 19.5 41.0 60-61 33.0763608328945 37.0 32.0 40.0 18.0 41.0 62-63 32.83217837685209 37.0 31.0 40.0 17.5 41.0 64-65 32.55239035618399 36.0 31.0 39.5 14.0 41.0 66-67 32.26857809762892 36.0 31.0 39.0 13.0 41.0 68-69 31.986549861796494 35.5 31.0 39.0 11.5 40.5 70-71 31.66045787216693 35.0 31.0 39.0 8.0 40.0 72-73 31.342778714142913 35.0 30.0 38.0 7.5 40.0 74-75 31.008777436654547 35.0 30.0 37.0 7.0 39.5 76-77 30.68602172029285 35.0 30.0 37.0 4.0 39.0 78-79 30.355913343124254 34.5 30.0 36.0 2.0 39.0 80-81 30.035129434792395 34.0 30.0 36.0 2.0 38.5 82-83 29.72760090126979 34.0 29.0 35.5 2.0 37.0 84-85 29.747095308956048 34.0 30.0 35.0 2.0 37.0 86-87 29.61374256989489 34.0 30.0 35.0 2.0 36.5 88-89 29.427639480122544 34.0 30.0 35.0 2.0 36.0 90-91 29.246680112775856 34.0 29.5 35.0 2.0 36.0 92-93 29.10953515722253 34.0 29.0 35.0 2.0 36.0 94-95 28.97369360864545 34.0 29.0 35.0 2.0 35.0 96-97 28.857426591147153 34.0 29.0 35.0 2.0 35.0 98-99 28.73892222448456 34.0 29.0 35.0 2.0 35.0 100-101 28.619744347811782 34.0 29.0 35.0 2.0 35.0 102-103 27.62189334940952 32.5 26.5 34.5 2.0 35.0 104-105 28.286846231122716 34.0 29.0 35.0 2.0 35.0 106-107 28.39899854481963 34.0 29.0 35.0 2.0 35.0 108-109 28.341904584129686 34.0 29.0 35.0 2.0 35.0 110-111 28.21313109009433 34.0 29.0 35.0 2.0 35.0 112-113 28.14895499407984 34.0 29.0 35.0 2.0 35.0 114-115 28.02004268595543 34.0 29.0 35.0 2.0 35.0 116-117 27.878481722770072 34.0 28.0 35.0 2.0 35.0 118-119 27.660718678170873 34.0 27.5 35.0 2.0 35.0 120-121 27.438833453248435 34.0 27.0 35.0 2.0 35.0 122-123 27.11674028133155 34.0 27.0 35.0 2.0 35.0 124-125 25.11003870096928 31.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5676425003947081 1101 2 0.6745216490527532 1101 3 0.5503389620283592 1101 4 0.6011292104852828 1101 5 0.5067862949789372 1101 6 0.5861063639586774 1101 7 0.5495684752003527 1101 8 0.4468575416937597 1101 9 0.6170109264892218 1101 10-11 0.7334026452311093 1101 12-13 0.6587208868644439 1101 14-15 0.8070924270164497 1101 16-17 0.8196825975842117 1101 18-19 0.7645508419502534 1101 20-21 0.687789115050002 1101 22-23 0.8578428260083868 1101 24-25 0.8845960224250646 1101 26-27 0.8284675308112597 1101 28-29 0.9211277960054929 1101 30-31 0.9115612469307237 1101 32-33 0.958939115450768 1101 34-35 0.8891081222528783 1101 36-37 0.8363951888240848 1101 38-39 0.9512532362300377 1101 40-41 0.8622569640438158 1101 42-43 0.9979416268198591 1101 44-45 0.918199022953857 1101 46-47 0.8529628463501737 1101 48-49 0.866732220940797 1101 50-51 0.889223917536107 1101 52-53 0.8277590329249733 1101 54-55 0.8278177723956546 1101 56-57 0.8303949887104025 1101 58-59 0.873420548630726 1101 60-61 0.9064919158583962 1101 62-63 0.8414830023995705 1101 64-65 0.8218972125789463 1101 66-67 0.9166396223241549 1101 68-69 0.8557413284098914 1101 70-71 0.793824302863861 1101 72-73 0.8010461407883831 1101 74-75 0.7681203107930621 1101 76-77 0.7284741970608586 1101 78-79 0.7900314659361563 1101 80-81 0.7655961562514406 1101 82-83 0.781622330767 1101 84-85 0.5540013039654959 1101 86-87 0.6478573340670053 1101 88-89 0.6719658378201885 1101 90-91 0.6999265356218345 1101 92-93 0.6798787240986961 1101 94-95 0.7820386458025048 1101 96-97 0.8289981945374514 1101 98-99 0.8461786558584166 1101 100-101 0.8487479249994614 1101 102-103 0.9290309686648186 1101 104-105 0.9199825518058908 1101 106-107 0.8594230116546662 1101 108-109 0.8508289803488225 1101 110-111 0.8636097344786648 1101 112-113 0.8403226055873532 1101 114-115 0.8998759406649768 1101 116-117 0.9170494707302943 1101 118-119 0.9673070831575146 1101 120-121 1.0286271377402407 1101 122-123 0.9758482761458751 1101 124-125 0.8483646297432337 1102 1 0.431055623280006 1102 2 0.37658738724792684 1102 3 0.46178188067983683 1102 4 0.5185954100282686 1102 5 0.4275099318601505 1102 6 0.3504045490454786 1102 7 0.42481001754394043 1102 8 0.40102289976432814 1102 9 0.5745576839814817 1102 10-11 0.6453378345275951 1102 12-13 0.5229861765528057 1102 14-15 0.6340534452924445 1102 16-17 0.6013269652576909 1102 18-19 0.5503210584062259 1102 20-21 0.4713694958553347 1102 22-23 0.6250936680222878 1102 24-25 0.7362890234600812 1102 26-27 0.5840331576146056 1102 28-29 0.5605466555698015 1102 30-31 0.5212928313092604 1102 32-33 0.5997262959534808 1102 34-35 0.6332669167148524 1102 36-37 0.5669587657084278 1102 38-39 0.5935244807397382 1102 40-41 0.491556572470607 1102 42-43 0.6728483555049465 1102 44-45 0.6091563273954534 1102 46-47 0.5724945713608847 1102 48-49 0.6055917813762761 1102 50-51 0.5073178177506961 1102 52-53 0.5079792096976163 1102 54-55 0.5359240997607628 1102 56-57 0.5984082652235898 1102 58-59 0.7688934378716681 1102 60-61 0.6980271427496234 1102 62-63 0.6484597276964905 1102 64-65 0.5695519891996597 1102 66-67 0.5238963162766623 1102 68-69 0.46211858776873527 1102 70-71 0.4677396249641532 1102 72-73 0.40854582749887314 1102 74-75 0.4067446333528473 1102 76-77 0.48113338680909834 1102 78-79 0.45675389297837654 1102 80-81 0.5223992766149514 1102 82-83 0.46331042359971164 1102 84-85 0.5081769116311108 1102 86-87 0.5671447706162134 1102 88-89 0.5645259081826914 1102 90-91 0.5360700307260089 1102 92-93 0.5939860539902249 1102 94-95 0.5155015043642805 1102 96-97 0.60470524254578 1102 98-99 0.5983571782767925 1102 100-101 0.6419330466099389 1102 102-103 0.567180618940899 1102 104-105 0.5963436593500546 1102 106-107 0.5274734381151731 1102 108-109 0.57476812792288 1102 110-111 0.5784740259725076 1102 112-113 0.5568004347463287 1102 114-115 0.5454387555982905 1102 116-117 0.6128510625504191 1102 118-119 0.6967057838956414 1102 120-121 0.6199159295566581 1102 122-123 0.6053263822373047 1102 124-125 0.5806790501885857 1103 1 0.2851699880212415 1103 2 0.2719803088164241 1103 3 0.32664063304525826 1103 4 0.2525618285160718 1103 5 0.22671940833954096 1103 6 0.19953596306913823 1103 7 0.2373456129665854 1103 8 0.2126218898778589 1103 9 0.3600030364182132 1103 10-11 0.4779707401231974 1103 12-13 0.3023522680865085 1103 14-15 0.3727241779022208 1103 16-17 0.4173374211578036 1103 18-19 0.4274412625747459 1103 20-21 0.40654247062835935 1103 22-23 0.42350647016891685 1103 24-25 0.4757155851657018 1103 26-27 0.39789574270484707 1103 28-29 0.4634830917315682 1103 30-31 0.4138961793693099 1103 32-33 0.4674587149621843 1103 34-35 0.44045887395132866 1103 36-37 0.45706725065767984 1103 38-39 0.5612127027994305 1103 40-41 0.6177225838515312 1103 42-43 0.668566417172606 1103 44-45 0.6014627305831084 1103 46-47 0.5341676835661175 1103 48-49 0.47055920609849977 1103 50-51 0.46889145979072566 1103 52-53 0.5359116214022279 1103 54-55 0.5375397691469246 1103 56-57 0.5034915750909335 1103 58-59 0.5025296346323032 1103 60-61 0.5630735068988457 1103 62-63 0.516809990980093 1103 64-65 0.4209474066523953 1103 66-67 0.48568521271525356 1103 68-69 0.5348428353273889 1103 70-71 0.529572189766327 1103 72-73 0.5903205266136347 1103 74-75 0.49887257889509584 1103 76-77 0.4802284005186799 1103 78-79 0.5406593319301258 1103 80-81 0.40703628979979456 1103 82-83 0.44348682456731225 1103 84-85 0.4425994032290035 1103 86-87 0.4398760940115025 1103 88-89 0.5077570355208394 1103 90-91 0.43206738972104475 1103 92-93 0.4814207376510744 1103 94-95 0.4938511862039121 1103 96-97 0.5387551895430782 1103 98-99 0.4275298916402974 1103 100-101 0.5720119235677465 1103 102-103 0.5396036809305151 1103 104-105 0.4952790162365126 1103 106-107 0.4704854427705705 1103 108-109 0.507510823112657 1103 110-111 0.44753140440026584 1103 112-113 0.48542451248490437 1103 114-115 0.47512653941262073 1103 116-117 0.4313304087959722 1103 118-119 0.4440332440042596 1103 120-121 0.4731746346377079 1103 122-123 0.561739468858999 1103 124-125 0.5888848702984752 1104 1 0.3292116170266617 1104 2 0.38144165620249026 1104 3 0.41195112101784304 1104 4 0.43766625443419116 1104 5 0.2678880091461693 1104 6 0.3430882505754269 1104 7 0.28411480038857206 1104 8 0.2082357101354475 1104 9 0.4492535081982254 1104 10-11 0.5305135072121843 1104 12-13 0.43495573135471943 1104 14-15 0.5476596860974041 1104 16-17 0.5873738598883875 1104 18-19 0.5102080591694502 1104 20-21 0.5167431162290441 1104 22-23 0.5549596347589159 1104 24-25 0.5735088907284762 1104 26-27 0.431558876010385 1104 28-29 0.5547770877321199 1104 30-31 0.5008433473783285 1104 32-33 0.6793680384757437 1104 34-35 0.6623439569313305 1104 36-37 0.6099279972922034 1104 38-39 0.7398459344529229 1104 40-41 0.6082033143611483 1104 42-43 0.7316555501570008 1104 44-45 0.7132208694301454 1104 46-47 0.5858692027708443 1104 48-49 0.5554537399022905 1104 50-51 0.5951810391402006 1104 52-53 0.6383962791126692 1104 54-55 0.6133687928574076 1104 56-57 0.6243966943586443 1104 58-59 0.6702185366290436 1104 60-61 0.6312605583242288 1104 62-63 0.5781197288892059 1104 64-65 0.636585154514357 1104 66-67 0.6846623088363373 1104 68-69 0.6131421389210132 1104 70-71 0.6293664056026387 1104 72-73 0.6447004296490881 1104 74-75 0.5873718877323952 1104 76-77 0.5456889446795721 1104 78-79 0.5689142152866786 1104 80-81 0.5778967109396476 1104 82-83 0.6034092712917207 1104 84-85 0.5923187999933646 1104 86-87 0.583520363365956 1104 88-89 0.6239233558107458 1104 90-91 0.5634788706940412 1104 92-93 0.5684565286544903 1104 94-95 0.7070703781144374 1104 96-97 0.6428705586792987 1104 98-99 0.6605569208682454 1104 100-101 0.6836508472701759 1104 102-103 0.6592826735836859 1104 104-105 0.6468069020325835 1104 106-107 0.6912754065154907 1104 108-109 0.6644041032665555 1104 110-111 0.7407598222846872 1104 112-113 0.6813814553625264 1104 114-115 0.5567188245846211 1104 116-117 0.5799139692848811 1104 118-119 0.5923796186713517 1104 120-121 0.6561303848805871 1104 122-123 0.6876496668827627 1104 124-125 0.7255683259983918 1105 1 0.39238026011924987 1105 2 0.44094992940900823 1105 3 0.4453062833421306 1105 4 0.43693750050962876 1105 5 0.3272209968340789 1105 6 0.2710016192718143 1105 7 0.2476943911676912 1105 8 0.21165150438842062 1105 9 0.37378956399367524 1105 10-11 0.43362174902738815 1105 12-13 0.4150320527818536 1105 14-15 0.43607785530102916 1105 16-17 0.36077431085667655 1105 18-19 0.30059809810215654 1105 20-21 0.24810377942116446 1105 22-23 0.19152668516399274 1105 24-25 0.3371015225418006 1105 26-27 0.2668618388660988 1105 28-29 0.35057411183773723 1105 30-31 0.38152865929016855 1105 32-33 0.4905009588437892 1105 34-35 0.5075561165838991 1105 36-37 0.4600385844096593 1105 38-39 0.47033044238157373 1105 40-41 0.3336684804966339 1105 42-43 0.5315337406932557 1105 44-45 0.5737578545561703 1105 46-47 0.48859053531210606 1105 48-49 0.4583306081482448 1105 50-51 0.4848232587104846 1105 52-53 0.5184638883617012 1105 54-55 0.5010232670688097 1105 56-57 0.5679759043499786 1105 58-59 0.5488395312116765 1105 60-61 0.5354552056699902 1105 62-63 0.5672084303237881 1105 64-65 0.5630140784152005 1105 66-67 0.564078138705284 1105 68-69 0.4397646441512819 1105 70-71 0.35647112598340414 1105 72-73 0.41551879373647793 1105 74-75 0.41595334242980186 1105 76-77 0.4075502684958643 1105 78-79 0.40509378334167323 1105 80-81 0.44567691663419495 1105 82-83 0.4461280524601392 1105 84-85 0.42092593655309685 1105 86-87 0.46983732050407667 1105 88-89 0.4296259890671692 1105 90-91 0.44284727969687054 1105 92-93 0.4319749068509253 1105 94-95 0.41345640702977704 1105 96-97 0.4579357759274849 1105 98-99 0.41324090636932453 1105 100-101 0.42536568193321145 1105 102-103 0.46689151909433946 1105 104-105 0.4748078092926242 1105 106-107 0.4754329227902616 1105 108-109 0.4669985089162694 1105 110-111 0.5244900416264997 1105 112-113 0.49824400039932115 1105 114-115 0.47598834622186814 1105 116-117 0.41868122473754354 1105 118-119 0.5369237396845676 1105 120-121 0.4948181674609984 1105 122-123 0.5708451825099061 1105 124-125 0.5876567726745847 1106 1 -0.3680695954532567 1106 2 -0.41315350336428835 1106 3 -0.17572559225206774 1106 4 -0.14212655630289106 1106 5 -0.13517983222951813 1106 6 -0.22102152542030495 1106 7 -0.3521832275517198 1106 8 -0.3824474396922426 1106 9 -0.3202922113547828 1106 10-11 -0.24720883963587426 1106 12-13 -0.27730231514154013 1106 14-15 -0.39680124941634887 1106 16-17 -0.2961910466189366 1106 18-19 -0.42176629582772307 1106 20-21 -0.46493362552025985 1106 22-23 -0.4116717339963145 1106 24-25 -4.144505356553612 1106 26-27 -1.184136051382545 1106 28-29 -0.8785150116998963 1106 30-31 -0.7825559278377909 1106 32-33 -5.704070785889133 1106 34-35 -1.3351391825636298 1106 36-37 -0.7052021889599303 1106 38-39 -0.2903521101575919 1106 40-41 -0.7977701407921174 1106 42-43 -0.3944988760429524 1106 44-45 -0.45623013109224075 1106 46-47 -0.5696336447034653 1106 48-49 -0.37861848980459456 1106 50-51 -0.3358549866018379 1106 52-53 -0.2261338374770574 1106 54-55 -0.3139906599604032 1106 56-57 -0.21889071599120058 1106 58-59 -0.10267487131903152 1106 60-61 -0.06983808559149196 1106 62-63 -0.12797824132425717 1106 64-65 -0.03927448491027263 1106 66-67 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2216 30-31 -1.1116926849364503 2216 32-33 -1.0463776514843701 2216 34-35 -1.2673868572869438 2216 36-37 -1.2152083774324183 2216 38-39 -1.0929800645298755 2216 40-41 -1.2981533073225506 2216 42-43 -0.971082404411284 2216 44-45 -0.8982394538644343 2216 46-47 -1.1138674747617117 2216 48-49 -1.2702358717967144 2216 50-51 -1.305555273213919 2216 52-53 -1.5479850644066957 2216 54-55 -1.419948244770147 2216 56-57 -1.4057177648488661 2216 58-59 -1.409479441408692 2216 60-61 -1.6718409997181034 2216 62-63 -1.6321118026456531 2216 64-65 -1.5483558332884968 2216 66-67 -1.5571709952850803 2216 68-69 -1.5019274675527576 2216 70-71 -1.5279427767638012 2216 72-73 -1.6870592923595247 2216 74-75 -1.5288228415542093 2216 76-77 -1.3713373973410476 2216 78-79 -1.2685636434601157 2216 80-81 -1.3573273395267904 2216 82-83 -1.3885546888225484 2216 84-85 -1.5077688939795095 2216 86-87 -1.439215298645422 2216 88-89 -1.354674255686291 2216 90-91 -1.2733188805643927 2216 92-93 -1.2394520304828625 2216 94-95 -1.251882611099706 2216 96-97 -1.472617438162274 2216 98-99 -1.324450253310527 2216 100-101 -1.4211889646844504 2216 102-103 -1.3723527884987021 2216 104-105 -1.3683316077527614 2216 106-107 -1.384800159441454 2216 108-109 -1.5017328664492595 2216 110-111 -1.449813514949419 2216 112-113 -1.4153756249135796 2216 114-115 -1.4629206069078577 2216 116-117 -1.3648971165823873 2216 118-119 -1.3483928043658153 2216 120-121 -1.397667004946907 2216 122-123 -1.3936617227596955 2216 124-125 -1.4099412078124125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 136168.0 3 24896.0 4 17715.0 5 14582.0 6 13601.0 7 13570.0 8 14260.0 9 16127.0 10 18966.0 11 21712.0 12 22880.0 13 22865.0 14 22342.0 15 21932.0 16 20836.0 17 19180.0 18 17389.0 19 16158.0 20 15616.0 21 16126.0 22 19170.0 23 27001.0 24 40540.0 25 56703.0 26 66537.0 27 63157.0 28 72299.0 29 87807.0 30 103350.0 31 126272.0 32 161080.0 33 209186.0 34 284098.0 35 407336.0 36 655811.0 37 869173.0 38 275376.0 39 744.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.325216563901776 18.127867093227586 9.274534916264118 22.272381426606525 2 18.207204378252285 21.28955081045517 38.07521881292741 22.428025998365133 3 17.092624884705803 29.799222990005635 29.689492570954062 23.4186595543345 4 10.548366315228078 16.856840591593137 36.870472803488056 35.72432028969073 5 10.516602821005442 44.325104715440915 33.87118038170825 11.287112081845395 6 30.088846244906602 37.95003177530498 19.290326600291586 12.67079537949683 7 28.375980830121534 35.22716879187648 21.006493385120915 15.390356992881078 8 27.058181423962658 35.77057894822101 19.30913998257959 17.86209964523674 9 27.97273136446355 15.01242859229183 18.133034535386972 38.881805507857656 10-11 23.63293324014177 28.496680455578637 28.554511892578322 19.315874411701266 12-13 24.543272735429024 27.13702004852318 28.017278440080595 20.302428775967194 14-15 22.211878157243383 28.33463281428446 25.232239904328964 24.221249124143192 16-17 22.23784284246552 30.818049334548704 25.474675716712948 21.46943210627283 18-19 21.417596801197856 29.637991457698966 27.30338825999339 21.64102348110979 20-21 23.019478423752226 28.760463263344395 26.849991688454878 21.3700666244485 22-23 22.614960855977884 29.13572613177444 26.59578580997708 21.653527202270595 24-25 22.103100316647225 28.87943318265041 27.063960845450957 21.95350565525141 26-27 21.95829006253176 29.09114992793807 26.845728657868705 22.10483135166146 28-29 22.00544694154083 29.05434655019144 26.949289063973904 21.990917444293835 30-31 22.155211375752657 28.66457596813186 27.203616567035127 21.976596089080356 32-33 21.784043666329847 28.989628586228676 27.10181221549204 22.12451553194944 34-35 22.21226592897886 28.98254533446728 27.08589016302028 21.719298573533578 36-37 22.44963671444514 28.632917853153582 26.79900741324835 22.11843801915293 38-39 22.037848843874453 28.676324721996544 27.17601569080931 22.109810743319695 40-41 22.058554519413416 28.676442367840373 27.295606716354303 21.969396396391904 42-43 21.920002372567044 28.58105911442689 27.32319983033155 22.175738682674517 44-45 21.93034256510813 28.709931984419327 27.29861771973912 22.061107730733415 46-47 22.069521276741565 28.729900135251423 26.909588396539135 22.29099019146787 48-49 22.068719307274293 28.643121402697286 27.006458478450764 22.28170081157766 50-51 22.057196090396346 28.563216793795537 27.198229944477664 22.181357171330454 52-53 22.033461959690484 28.520671452263308 27.328866239263466 22.117000348782742 54-55 21.8363530830556 28.693200722038398 27.260338803781853 22.21010739112415 56-57 22.11145546251242 28.893214776542166 27.025418217483683 21.969911543461734 58-59 22.302686472535655 28.43494956322018 26.93510457694213 22.327259387302036 60-61 22.161718889990713 28.461314931702912 27.35732676260416 22.019639415702212 62-63 21.83916582441057 28.57841916204376 27.375451911777006 22.20696310176867 64-65 21.739826080195108 28.71073651869801 27.296469891009156 22.252967510097722 66-67 21.879280318360983 29.054751190763383 26.977843527012237 22.088124963863397 68-69 21.98388434309041 28.687439370027718 27.12621563678527 22.202460650096604 70-71 21.870727857506093 28.47428337662043 27.26768174008028 22.387307025793195 72-73 21.88834949883578 28.6555555472483 27.128736115150513 22.327358838765406 74-75 21.6184771003819 28.920892319010626 27.20894113378106 22.25168944682641 76-77 21.52360433464193 28.883000430523424 27.267833545064462 22.325561689770176 78-79 21.87591626435665 28.760679133116255 27.07838506448445 22.28501953804264 80-81 21.696448620117913 28.815943365732487 27.045960514415633 22.44164749973396 82-83 21.848366821318617 28.757125908569307 27.12134913422446 22.273158135887613 84-85 21.70091126476771 28.612629608815332 27.282840982865032 22.403618143551927 86-87 21.609921857768928 28.66141398684878 27.30151985455651 22.427144300825784 88-89 21.79134104787414 28.660833721178726 27.310446405374133 22.237378825573 90-91 21.75360565106202 28.66655676164479 27.298650448573238 22.28118713871995 92-93 21.71064485812912 28.612341207863217 27.214566671576257 22.46244726243141 94-95 21.453495884852376 28.59931961031797 27.359385987003037 22.587798517826617 96-97 21.607657670230815 28.635068156126074 27.360879061504995 22.39639511213811 98-99 21.39925982230751 28.69974205928898 27.18769859565613 22.71329952274738 100-101 21.376888030138453 28.710070420332613 27.256717270831743 22.65632427869719 102-103 21.403787546779853 28.42754765710386 27.355073569900597 22.813591226215685 104-105 21.189470684411493 28.475249962648437 27.422752043308122 22.912527309631944 106-107 21.192426559775345 28.712484258759208 27.38173415452372 22.713355026941727 108-109 21.302564019491356 28.813179158626784 26.96921344856354 22.915043373318323 110-111 21.22282288030024 28.975187434105077 27.09527011981303 22.706719565781654 112-113 21.272823538838068 28.799667742582248 27.154492596543623 22.773016122036065 114-115 20.996619857731474 28.894260088277964 27.058820157640284 23.05029989635028 116-117 20.906554206051993 28.901279748728083 27.29850664621747 22.893659399002452 118-119 20.946984414100108 28.943085135414325 26.992598448313377 23.11733200217219 120-121 20.86511645410215 28.916674236672396 27.225939936147782 22.99226937307767 122-123 20.993314345391386 28.742620178981106 27.0155996946079 23.248465781019608 124-125 21.038079875590412 28.788009627288364 26.700398312545442 23.473512184575778 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6176.0 1 6531.5 2 6422.5 3 4977.0 4 3339.5 5 2390.5 6 2009.5 7 1884.5 8 1783.5 9 1712.0 10 1624.5 11 1534.5 12 1523.0 13 1574.5 14 1673.5 15 1774.0 16 2011.5 17 2336.0 18 2769.5 19 3268.5 20 4145.5 21 5705.5 22 7963.0 23 11137.5 24 14455.5 25 18943.5 26 26091.5 27 33429.5 28 39209.5 29 45625.5 30 55608.5 31 65152.5 32 74334.5 33 84266.5 34 94271.5 35 102150.5 36 109859.5 37 118654.0 38 121208.5 39 122789.5 40 125679.5 41 129565.5 42 133137.0 43 138411.0 44 147127.5 45 151352.0 46 149890.5 47 146154.0 48 144671.0 49 143518.5 50 140966.5 51 135141.5 52 128614.5 53 125703.0 54 120320.5 55 110552.5 56 99129.0 57 89312.0 58 82457.0 59 74298.5 60 62567.5 61 50661.5 62 40942.5 63 32221.0 64 24534.5 65 18950.5 66 14279.5 67 11026.5 68 8472.5 69 5834.0 70 4092.0 71 3079.0 72 2543.0 73 2214.0 74 1813.5 75 1377.0 76 916.0 77 551.0 78 383.0 79 274.5 80 159.5 81 102.0 82 81.0 83 54.5 84 38.0 85 27.0 86 19.0 87 16.5 88 14.5 89 13.5 90 10.0 91 7.5 92 8.0 93 8.5 94 10.0 95 5.5 96 2.0 97 3.5 98 4.5 99 3.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.968695803004616E-5 2 2.492173950751154E-5 3 0.0 4 1.495304370450692E-4 5 4.984347901502307E-4 6 2.741391345826269E-4 7 3.9874783212018463E-4 8 6.479652271952999E-4 9 9.719478407929499E-4 10-11 9.345652315316827E-4 12-13 0.0011464000173455307 14-15 0.0015326869797119595 16-17 0.001457921761189425 18-19 0.0014703826309431808 20-21 0.0017445217655258074 22-23 0.08453454040947914 24-25 0.0012834695846368441 26-27 9.968695803004614E-4 28-29 8.473391432553922E-4 30-31 6.604260969490558E-4 32-33 0.0010342521895617288 34-35 5.856608784265212E-4 36-37 3.7382609261267307E-4 38-39 5.233565296577422E-4 40-41 6.728869667028115E-4 42-43 7.351913154715904E-4 44-45 6.105826179340326E-4 46-47 8.473391432553922E-4 48-49 1.495304370450692E-4 50-51 7.850347944866134E-4 52-53 8.59800013009148E-4 54-55 7.974956642403693E-4 56-57 6.355043574415442E-4 58-59 3.2398261359764996E-4 60-61 4.4859131113520763E-4 62-63 6.105826179340326E-4 64-65 5.482782691652538E-4 66-67 2.2429565556760382E-4 68-69 8.722608827629038E-5 70-71 2.616782648288711E-4 72-73 9.968695803004616E-5 74-75 1.2460869753755767E-4 76-77 8.722608827629038E-5 78-79 1.1214782778380191E-4 80-81 1.9937391606009231E-4 82-83 6.230434876877884E-5 84-85 1.495304370450692E-4 86-87 1.495304370450692E-4 88-89 2.1183478581384804E-4 90-91 1.3706956729131345E-4 92-93 9.968695803004616E-5 94-95 1.8691304630633654E-4 96-97 2.2429565556760382E-4 98-99 2.741391345826269E-4 100-101 3.115217438438942E-4 102-103 4.112087018739404E-4 104-105 2.866000043363827E-4 106-107 2.990608740901384E-4 108-109 3.3644348335140574E-4 110-111 4.3613044138145186E-4 112-113 2.367565253213596E-4 114-115 1.9937391606009231E-4 116-117 3.8628696236642885E-4 118-119 5.981217481802768E-4 120-121 3.613652228589173E-4 122-123 3.613652228589173E-4 124-125 2.1183478581384804E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4012561.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.971563695667946 #Duplication Level Percentage of deduplicated Percentage of total 1 51.74123562265669 14.990245035343616 2 13.802309392088317 7.997489714004052 3 9.54174788007861 8.293180694271062 4 6.859253917094808 7.948932470554884 5 4.702974604856815 6.812626416185901 6 3.340143839080107 5.806151399196131 7 2.246787252357071 4.556505799459436 8 1.5107886547689207 3.501592779386421 9 1.1598302963282547 3.0241887575615514 >10 4.714747389735041 22.866074747287747 >50 0.23669911032927526 4.723220141335025 >100 0.13411484380976646 7.134068656410957 >500 0.007459064160043084 1.431491583372645 >1k 0.0018213993538694715 0.7238872510954516 >5k 8.673330256521286E-5 0.19034455453513063 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7575 0.1887821767693999 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.4921739507511536E-5 0.0 4 0.0 2.4921739507511536E-5 0.0 2.4921739507511536E-5 0.0 5 0.0 2.4921739507511536E-5 0.0 2.4921739507511536E-5 0.0 6 0.0 2.4921739507511536E-5 0.0 2.4921739507511536E-5 0.0 7 0.0 2.4921739507511536E-5 0.0 2.4921739507511536E-5 0.0 8 0.0 2.4921739507511536E-5 0.0 2.4921739507511536E-5 0.0 9 0.0 2.4921739507511536E-5 0.0 9.968695803004614E-5 0.0 10-11 0.0 2.4921739507511536E-5 0.0 9.968695803004614E-5 0.0 12-13 0.0 2.4921739507511536E-5 0.0 9.968695803004614E-5 0.0 14-15 0.0 2.4921739507511536E-5 0.0 9.968695803004614E-5 0.0 16-17 0.0 2.4921739507511536E-5 0.0 9.968695803004614E-5 0.0 18-19 0.0 2.4921739507511536E-5 0.0 1.3706956729131345E-4 0.0 20-21 0.0 2.4921739507511536E-5 0.0 1.7445217655258076E-4 0.0 22-23 0.0 2.4921739507511536E-5 0.0 3.2398261359764996E-4 0.0 24-25 0.0 2.4921739507511536E-5 0.0 4.8597392039647497E-4 0.0 26-27 0.0 2.4921739507511536E-5 0.0 5.607391389190095E-4 0.0 28-29 0.0 2.4921739507511536E-5 0.0 7.725739247328577E-4 0.0 30-31 0.0 2.4921739507511536E-5 0.0 0.002429869601982375 0.0 32-33 0.0 2.4921739507511536E-5 0.0 0.006753791406535627 0.0 34-35 0.0 2.4921739507511536E-5 0.0 0.012884539325383465 0.0 36-37 2.4921739507511536E-5 2.4921739507511536E-5 0.0 0.02246694816602165 0.0 38-39 2.4921739507511536E-5 6.230434876877884E-5 0.0 0.04120809627567032 0.0 40-41 2.4921739507511536E-5 9.968695803004614E-5 0.0 0.06396164444602837 0.0 42-43 2.4921739507511536E-5 9.968695803004614E-5 0.0 0.08916998395787629 0.0 44-45 2.4921739507511536E-5 9.968695803004614E-5 0.0 0.11928790615270396 0.0 46-47 2.4921739507511536E-5 9.968695803004614E-5 0.0 0.1594243676295513 0.0 48-49 2.4921739507511536E-5 1.1214782778380191E-4 0.0 0.2036604552553843 0.0 50-51 2.4921739507511536E-5 1.2460869753755767E-4 0.0 0.2479962298392473 0.0 52-53 2.4921739507511536E-5 1.7445217655258076E-4 0.0 0.29649393492086473 0.0 54-55 2.4921739507511536E-5 1.7445217655258076E-4 0.0 0.35322827490971476 0.0 56-57 2.4921739507511536E-5 1.7445217655258076E-4 0.0 0.4277941195161893 0.0 58-59 2.4921739507511536E-5 1.7445217655258076E-4 0.0 0.5186463208908225 0.0 60-61 2.4921739507511536E-5 1.7445217655258076E-4 0.0 0.6060593222134193 0.0 62-63 2.4921739507511536E-5 1.7445217655258076E-4 0.0 0.6959021931379984 0.0 64-65 2.4921739507511536E-5 1.7445217655258076E-4 0.0 0.7952900902939544 0.0 66-67 2.4921739507511536E-5 1.7445217655258076E-4 0.0 0.9209953443698427 0.0 68-69 2.4921739507511536E-5 1.7445217655258076E-4 0.0 1.0831112598662052 0.0 70-71 2.4921739507511536E-5 1.7445217655258076E-4 0.0 1.2459125231990242 0.0 72-73 2.4921739507511536E-5 1.7445217655258076E-4 0.0 1.4026328820920106 0.0 74-75 2.4921739507511536E-5 1.7445217655258076E-4 0.0 1.56613195413104 0.0 76-77 2.4921739507511536E-5 1.7445217655258076E-4 0.0 1.7640105658206817 0.0 78-79 2.4921739507511536E-5 1.7445217655258076E-4 0.0 2.0193836305541524 0.0 80-81 2.4921739507511536E-5 1.7445217655258076E-4 0.0 2.2834668432454985 0.0 82-83 2.4921739507511536E-5 1.7445217655258076E-4 0.0 2.545132647204616 0.0 84-85 2.4921739507511536E-5 1.7445217655258076E-4 0.0 2.813901146923374 0.0 86-87 2.4921739507511536E-5 1.7445217655258076E-4 0.0 3.1108685948948813 0.0 88-89 2.4921739507511536E-5 1.7445217655258076E-4 0.0 3.4718724525309397 0.0 90-91 2.4921739507511536E-5 1.7445217655258076E-4 0.0 3.8562653626947974 0.0 92-93 2.4921739507511536E-5 1.7445217655258076E-4 0.0 4.237069542369574 0.0 94-95 2.4921739507511536E-5 1.7445217655258076E-4 0.0 4.637088383204642 0.0 96-97 2.4921739507511536E-5 1.7445217655258076E-4 0.0 5.055050876485118 0.0 98-99 2.4921739507511536E-5 1.7445217655258076E-4 0.0 5.532165118486672 0.0 100-101 2.4921739507511536E-5 1.7445217655258076E-4 0.0 6.060979509096559 0.0 102-103 2.4921739507511536E-5 1.7445217655258076E-4 0.0 6.583987134401196 0.0 104-105 2.4921739507511536E-5 1.7445217655258076E-4 0.0 7.12791656002239 0.0 106-107 2.4921739507511536E-5 1.7445217655258076E-4 0.0 7.688543551113615 0.0 108-109 2.4921739507511536E-5 1.7445217655258076E-4 0.0 8.279276003529915 0.0 110-111 2.4921739507511536E-5 1.7445217655258076E-4 0.0 8.934206856917566 0.0 112-113 2.4921739507511536E-5 1.7445217655258076E-4 0.0 9.581499197146162 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5920 0.0 62.810623 1 GTACATG 35075 0.0 55.245243 1 TACATGG 35005 0.0 53.656124 2 ACATGGG 35465 0.0 51.416832 3 CATGGGA 24805 0.0 48.281345 4 ATGGGAG 9085 0.0 43.613815 5 GTATCAA 8610 0.0 43.255962 2 CATGGGG 10605 0.0 41.45802 4 ATGGGAT 9700 0.0 41.40062 5 TACCTGG 3565 0.0 37.21518 2 TCAACGC 10185 0.0 36.45007 5 ATCAACG 10420 0.0 35.685116 4 CAACGCA 10650 0.0 35.08204 6 AACGCAG 10875 0.0 34.46605 7 GAGTACA 19785 0.0 32.566208 1 TATCAAC 11855 0.0 32.41946 3 AGTACAT 19690 0.0 31.03127 2 ATGGGGA 5750 0.0 30.73006 5 ATGGGAC 4585 0.0 30.363466 5 ATGGGGG 5625 0.0 30.037975 5 >>END_MODULE