FastQCFastQC Report
Thu 26 May 2016
SRR936235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4012561
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221820.5528140257556209No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185860.4631954504866094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183860.4582111025851071No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50320.12540619320179805No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46340.11548734087780846No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42420.10571801899086394No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114300.082.711441
GTATCAA188050.050.178142
GTACATG301250.048.131391
TACATGG304700.047.3412322
ACATGGG307100.046.060143
CATGGGA217000.042.8528754
ATGGGAT87800.037.360765
ATCAACG265900.036.8527684
TCAACGC266600.036.8006675
ATGGGAG81450.036.6188965
CATGGGG93550.036.400794
CAACGCA270950.036.2746666
AACGCAG275450.035.7468457
TATCAAC277200.035.4149063
TACCTGG38050.033.169572
GAGTACA188700.031.2534641
ACGCAGA316050.031.0606618
AGTACAT187100.030.4188442
CGCAGAG322850.030.388029
CAGAGTA354600.027.6503739
Produced by FastQC (version 0.11.5)