##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936235_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4012561 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.585890906082174 31.0 31.0 34.0 30.0 34.0 2 31.33590791516939 31.0 31.0 34.0 28.0 34.0 3 31.701210274435702 31.0 31.0 34.0 30.0 34.0 4 35.541676998804505 37.0 35.0 37.0 33.0 37.0 5 35.17948238045478 37.0 35.0 37.0 33.0 37.0 6 35.387056296465026 37.0 35.0 37.0 33.0 37.0 7 35.204181070393695 37.0 35.0 37.0 32.0 37.0 8 35.278118139512394 37.0 35.0 37.0 32.0 37.0 9 36.93205835375462 39.0 37.0 39.0 33.0 39.0 10-11 36.81591532191037 39.0 37.0 39.0 32.0 39.0 12-13 36.76009884958758 39.0 37.0 39.0 32.0 39.0 14-15 37.863047315666975 40.0 37.0 41.0 32.0 41.0 16-17 37.78353139055082 40.0 37.0 41.0 32.0 41.0 18-19 37.922540118393215 40.0 37.0 41.0 32.0 41.0 20-21 37.985906631699805 40.0 37.0 41.0 32.5 41.0 22-23 37.893087482034545 40.0 37.0 41.0 32.0 41.0 24-25 37.86202814112981 40.0 37.0 41.0 32.0 41.0 26-27 37.6870361596995 40.0 37.0 41.0 32.0 41.0 28-29 37.530532495331535 40.0 36.5 41.0 32.0 41.0 30-31 37.326128375369244 39.0 36.0 41.0 31.5 41.0 32-33 37.0962939878048 39.0 35.5 41.0 31.0 41.0 34-35 36.89507411849938 39.0 35.0 41.0 30.5 41.0 36-37 36.68907450877382 39.0 35.0 41.0 30.0 41.0 38-39 36.675863245443495 39.0 35.0 41.0 30.0 41.0 40-41 36.69816060117217 39.0 35.0 41.0 30.0 41.0 42-43 36.5840632703154 39.0 35.0 41.0 30.0 41.0 44-45 36.39673851687239 39.0 35.0 41.0 30.0 41.0 46-47 36.08171128114937 39.0 35.0 41.0 29.5 41.0 48-49 35.78464202787198 39.0 35.0 41.0 27.5 41.0 50-51 35.608339162943565 39.0 35.0 41.0 27.0 41.0 52-53 35.474531602136395 39.0 35.0 41.0 27.0 41.0 54-55 35.306280951242854 39.0 35.0 41.0 27.0 41.0 56-57 35.10453598088602 38.5 34.5 40.5 26.0 41.0 58-59 34.89143803670524 38.0 34.0 40.0 26.0 41.0 60-61 34.67646136719168 38.0 34.0 40.0 26.0 41.0 62-63 34.467243114808724 37.5 34.0 40.0 26.0 41.0 64-65 34.224008681737175 37.0 34.0 40.0 26.0 41.0 66-67 33.94495572777585 37.0 33.5 40.0 25.0 41.0 68-69 33.64283284416112 36.0 33.0 39.0 25.0 41.0 70-71 33.31566809825446 36.0 33.0 39.0 24.0 41.0 72-73 33.049380059268884 35.0 33.0 39.0 24.0 40.0 74-75 32.86089980937361 35.0 33.0 37.5 24.0 40.0 76-77 32.55006665319232 35.0 33.0 37.0 24.0 39.0 78-79 32.216015656833626 35.0 32.5 37.0 23.0 39.0 80-81 31.926983539938707 35.0 32.0 36.0 23.0 39.0 82-83 31.66278232281079 35.0 32.0 36.0 23.0 37.0 84-85 31.40828039748181 35.0 32.0 35.5 21.5 37.0 86-87 31.199307624233 35.0 32.0 35.0 21.0 36.5 88-89 31.033841105468554 35.0 32.0 35.0 20.0 36.0 90-91 30.892891347944616 35.0 32.0 35.0 20.0 36.0 92-93 30.75601978387369 35.0 32.0 35.0 20.0 36.0 94-95 30.631866780343028 34.5 32.0 35.0 19.5 35.5 96-97 30.56621544195839 34.0 32.0 35.0 19.0 35.0 98-99 30.479273087686394 34.0 32.0 35.0 19.0 35.0 100-101 30.38718314811912 34.0 31.0 35.0 18.0 35.0 102-103 29.362199727306326 33.5 29.5 34.5 16.0 35.0 104-105 30.117125322206938 34.0 31.0 35.0 17.0 35.0 106-107 30.264880334529494 34.0 31.0 35.0 17.0 35.0 108-109 30.228966737203496 34.0 31.0 35.0 16.5 35.0 110-111 30.189356124430255 34.0 31.0 35.0 14.0 35.0 112-113 30.10254087102975 34.0 31.0 35.0 10.0 35.0 114-115 30.074171831904863 34.0 31.0 35.0 7.0 35.0 116-117 29.970678950425928 34.0 31.0 35.0 2.0 35.0 118-119 29.84048491723864 34.0 31.0 35.0 2.0 35.0 120-121 29.642699762072155 34.0 31.0 35.0 2.0 35.0 122-123 29.41628164655939 34.0 31.0 35.0 2.0 35.0 124-125 27.35837224655276 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18553403305422833 1101 2 0.6126473732146138 1101 3 0.3485331013698385 1101 4 0.2300845242997127 1101 5 0.5114469584752896 1101 6 0.2968907778807761 1101 7 0.4180870704246402 1101 8 0.3725638309019743 1101 9 0.29561557056719323 1101 10-11 0.3266699317390973 1101 12-13 0.43521172686493514 1101 14-15 0.5306932160535212 1101 16-17 0.581443837728763 1101 18-19 0.5148138824055764 1101 20-21 0.41507142754574744 1101 22-23 0.3685916144684569 1101 24-25 0.2859254687592525 1101 26-27 0.3737456549670881 1101 28-29 0.3999003218106125 1101 30-31 0.32754034525465414 1101 32-33 0.32299165140095454 1101 34-35 0.27672487460204565 1101 36-37 0.29720607451516656 1101 38-39 0.26118797508952696 1101 40-41 0.30252925886289006 1101 42-43 0.23989847022621547 1101 44-45 0.14774352416802827 1101 46-47 0.09504578295827315 1101 48-49 0.07077034573105578 1101 50-51 0.05818565142548948 1101 52-53 0.06233361446528107 1101 54-55 -0.03153376879368608 1101 56-57 -0.16572619403046218 1101 58-59 -0.07638589714259325 1101 60-61 0.03416675512624323 1101 62-63 -0.09598640683400106 1101 64-65 -0.09226182399634553 1101 66-67 -0.0571314562089853 1101 68-69 0.008899752041244824 1101 70-71 0.060573075552532885 1101 72-73 0.022280268362059985 1101 74-75 0.05455489469877506 1101 76-77 0.07783133561483879 1101 78-79 0.04924769577669963 1101 80-81 0.03278295507160678 1101 82-83 -0.05958118005317914 1101 84-85 -0.039740023422396575 1101 86-87 -0.011831268811377527 1101 88-89 -0.08648365695878724 1101 90-91 0.014412385172892783 1101 92-93 0.020534796484241724 1101 94-95 0.004013260701057675 1101 96-97 0.023369535555247012 1101 98-99 0.05451817222485289 1101 100-101 0.14110775213618254 1101 102-103 0.1804342963782446 1101 104-105 0.017994372550038662 1101 106-107 0.0053017824814567405 1101 108-109 0.0018084106871505412 1101 110-111 0.03526487770531617 1101 112-113 0.042274486533464994 1101 114-115 -0.025283254659168364 1101 116-117 0.06508223648721412 1101 118-119 0.09099300217434347 1101 120-121 0.10330874223450692 1101 122-123 0.1759335802245161 1101 124-125 0.12995556404184683 1102 1 0.20167134359142835 1102 2 0.6190572378890167 1102 3 0.40443555307297174 1102 4 0.2727458529257092 1102 5 0.4528411277320714 1102 6 0.28138990917925355 1102 7 0.41927699039270294 1102 8 0.30766629591024497 1102 9 0.3324721474035712 1102 10-11 0.3837519571209569 1102 12-13 0.3269848023777655 1102 14-15 0.3681038898657647 1102 16-17 0.4760404832877043 1102 18-19 0.4198641479667913 1102 20-21 0.32478013405459905 1102 22-23 0.34591815651035773 1102 24-25 0.2952283299820593 1102 26-27 0.2692181574308563 1102 28-29 0.3472795777132234 1102 30-31 0.2457895751813055 1102 32-33 0.2997979781472111 1102 34-35 0.3274897651363773 1102 36-37 0.2945605186622231 1102 38-39 0.20902962310064055 1102 40-41 0.20423167792203856 1102 42-43 0.274918242275227 1102 44-45 0.2867048263384646 1102 46-47 0.2654942278819803 1102 48-49 0.26527961832695723 1102 50-51 0.23441003323954135 1102 52-53 0.28689295923381053 1102 54-55 0.3442566103562399 1102 56-57 0.17317743966371069 1102 58-59 0.30575526646878615 1102 60-61 0.28903067144622696 1102 62-63 0.2663396788108443 1102 64-65 0.3016486788603885 1102 66-67 0.23060039736645876 1102 68-69 0.2981076439973691 1102 70-71 0.3046955129603788 1102 72-73 0.3086330679325755 1102 74-75 0.2736370879419283 1102 76-77 0.2734431165380542 1102 78-79 0.24636403989698863 1102 80-81 0.17917124918315608 1102 82-83 0.1308327024624809 1102 84-85 0.1984471249004578 1102 86-87 0.1070275799541065 1102 88-89 0.11267970534158067 1102 90-91 0.11639314842349435 1102 92-93 0.20138058533175496 1102 94-95 0.15183901217042362 1102 96-97 0.13120465476770704 1102 98-99 0.11251086445697567 1102 100-101 0.13310552584677993 1102 102-103 0.20947052889011886 1102 104-105 0.1602268426482958 1102 106-107 0.08657256208499575 1102 108-109 0.11818134587721829 1102 110-111 0.1570162395477368 1102 112-113 0.11836303220488631 1102 114-115 0.15263637149748632 1102 116-117 0.21380521430013033 1102 118-119 0.16123860181046368 1102 120-121 0.1746346105105765 1102 122-123 0.12656930652242693 1102 124-125 0.17274742996321635 1103 1 0.11335144754015403 1103 2 0.5835427368688464 1103 3 0.28355301724689497 1103 4 0.17567755584532563 1103 5 0.339943018818488 1103 6 0.1839269990570358 1103 7 0.226017824000742 1103 8 0.25314206122985183 1103 9 0.2337278260928386 1103 10-11 0.26741439093966335 1103 12-13 0.27752692679139557 1103 14-15 0.2710237716932937 1103 16-17 0.3996232977263503 1103 18-19 0.3451761455103082 1103 20-21 0.25444331462033887 1103 22-23 0.30276360197812124 1103 24-25 0.18660805085050924 1103 26-27 0.21853910536711396 1103 28-29 0.21719901889271398 1103 30-31 0.1315062721375142 1103 32-33 0.1796505124259795 1103 34-35 0.1438348035933643 1103 36-37 0.050384436767998864 1103 38-39 0.11574310241325492 1103 40-41 0.1261319569190249 1103 42-43 0.17126068345567802 1103 44-45 0.24550081206858465 1103 46-47 0.29660311359508285 1103 48-49 0.25643879894268196 1103 50-51 0.22313583483932575 1103 52-53 0.08170047245084788 1103 54-55 0.1271957591222872 1103 56-57 0.09193089348888606 1103 58-59 0.2534513188006642 1103 60-61 0.16744635438339373 1103 62-63 -0.024467235663074405 1103 64-65 0.06271390582055147 1103 66-67 -0.010115023776762655 1103 68-69 -0.08436749086399686 1103 70-71 -0.09440648047939248 1103 72-73 -0.004794359211537369 1103 74-75 0.04893164216662882 1103 76-77 0.08149050518192524 1103 78-79 0.06408282380454011 1103 80-81 0.006927569863435679 1103 82-83 -0.006016981299278967 1103 84-85 -0.03374198518273985 1103 86-87 -0.03042525581055955 1103 88-89 0.001661738723189643 1103 90-91 0.006698038895788017 1103 92-93 -0.02628937169905754 1103 94-95 0.021906793639757893 1103 96-97 -0.04890354666754604 1103 98-99 0.005129889880013394 1103 100-101 0.003038746287082006 1103 102-103 -0.0010354787234376772 1103 104-105 0.052033408255834246 1103 106-107 0.015661302446719816 1103 108-109 -0.0026174932530942385 1103 110-111 0.04426650696390766 1103 112-113 0.02839495876419207 1103 114-115 0.03320182334672239 1103 116-117 0.03222630802985549 1103 118-119 -0.006952821554744304 1103 120-121 -0.0768170412227498 1103 122-123 -0.021011694387702562 1103 124-125 0.05116660877213519 1104 1 0.17332027786040882 1104 2 0.4652920907425795 1104 3 0.28024099410898273 1104 4 0.18238151384370838 1104 5 0.44643683097616105 1104 6 0.27437112047693546 1104 7 0.3843879969925865 1104 8 0.29315734280630323 1104 9 0.2954099134827217 1104 10-11 0.2812016399333075 1104 12-13 0.2766292791610141 1104 14-15 0.2709840816405844 1104 16-17 0.43004251529949045 1104 18-19 0.377987724808321 1104 20-21 0.3545115428479235 1104 22-23 0.31428676604737404 1104 24-25 0.1948837591004775 1104 26-27 0.1975505463721703 1104 28-29 0.3001874394255566 1104 30-31 0.22791995871754978 1104 32-33 0.23047963581467457 1104 34-35 0.17387564266402933 1104 36-37 0.19717960093681342 1104 38-39 0.2271238293344524 1104 40-41 0.10237880754053208 1104 42-43 0.11012985888003612 1104 44-45 0.09788631714051377 1104 46-47 0.144111007203513 1104 48-49 0.08560499490666729 1104 50-51 0.13799302374693667 1104 52-53 0.05467779305936915 1104 54-55 0.10679002166841656 1104 56-57 0.19376196416702385 1104 58-59 0.16935589014024544 1104 60-61 0.17432052652561225 1104 62-63 0.07433169622142088 1104 64-65 0.022875157382628686 1104 66-67 0.0374127450635342 1104 68-69 0.08607640824328655 1104 70-71 0.12037482008086897 1104 72-73 0.06675049483720841 1104 74-75 0.1125503262377947 1104 76-77 0.18050878954949923 1104 78-79 0.14724304125045506 1104 80-81 0.0681554387323331 1104 82-83 0.08102741223611432 1104 84-85 0.18140559958995794 1104 86-87 0.0974599482331655 1104 88-89 0.07865255129153681 1104 90-91 0.06669779966807354 1104 92-93 0.11449248971729276 1104 94-95 0.14337359058484367 1104 96-97 0.11142630002252574 1104 98-99 0.15109060844757494 1104 100-101 0.06603511207979196 1104 102-103 0.11515860648005116 1104 104-105 0.05514437137332706 1104 106-107 0.06308540594388035 1104 108-109 0.0419476336529776 1104 110-111 0.08172322396465148 1104 112-113 0.14906259015487677 1104 114-115 0.14694459773335922 1104 116-117 0.18864364585310156 1104 118-119 0.1176071051690819 1104 120-121 0.16016131700576963 1104 122-123 0.20289900698083585 1104 124-125 0.24468102459798757 1105 1 0.18706278565957035 1105 2 0.5843855998596759 1105 3 0.24663086026634318 1105 4 0.143793740153086 1105 5 0.3714843217007342 1105 6 0.2630958238425478 1105 7 0.3260985525292739 1105 8 0.2641969640115036 1105 9 0.3300986381813473 1105 10-11 0.16656969429198654 1105 12-13 0.17896340641792108 1105 14-15 0.16709668598602434 1105 16-17 0.3101335448913858 1105 18-19 0.30477591061172404 1105 20-21 0.21491008982996362 1105 22-23 0.2977605764443325 1105 24-25 0.2257356088315703 1105 26-27 0.25880953164946874 1105 28-29 0.18133503669966444 1105 30-31 0.2418530261719809 1105 32-33 0.19420022226763933 1105 34-35 0.20908220795684684 1105 36-37 0.2187285660536702 1105 38-39 0.13443448344023778 1105 40-41 0.11663151886555312 1105 42-43 0.10854427325092075 1105 44-45 0.17245703220170583 1105 46-47 0.09282225606759908 1105 48-49 -0.07156855902213977 1105 50-51 0.06141887477565433 1105 52-53 0.01739337684183795 1105 54-55 0.026462058247915365 1105 56-57 0.04865384151376162 1105 58-59 0.0767002539104098 1105 60-61 -0.0522372850396593 1105 62-63 -0.029471987691948698 1105 64-65 0.00715211503793256 1105 66-67 0.021300745052926118 1105 68-69 0.08161555519245667 1105 70-71 0.030443284681552996 1105 72-73 -0.04232068230806618 1105 74-75 -0.014837406134518005 1105 76-77 -0.08976560214558305 1105 78-79 -0.041986532717643854 1105 80-81 -0.09256699572539873 1105 82-83 -0.06433477022346423 1105 84-85 -0.04325103189788493 1105 86-87 0.038059553056911 1105 88-89 0.022794121841258175 1105 90-91 -0.03147345755155939 1105 92-93 0.01593775694435351 1105 94-95 0.05230187048545787 1105 96-97 0.024742815702577303 1105 98-99 0.04482389303628409 1105 100-101 0.02447690954398496 1105 102-103 -0.0763299175756238 1105 104-105 -0.07879493550671768 1105 106-107 -0.015342750887452183 1105 108-109 -0.007424746151759365 1105 110-111 0.04621196924665938 1105 112-113 0.05834294482553659 1105 114-115 0.05979082392991941 1105 116-117 0.0468697725077547 1105 118-119 0.05799450605710277 1105 120-121 0.04982267068832513 1105 122-123 0.03434517928929637 1105 124-125 0.2077862409500959 1106 1 0.16187237233738827 1106 2 0.5719078993490285 1106 3 0.2983983253025606 1106 4 0.14227398767241795 1106 5 0.2528496750016984 1106 6 0.2145565928898634 1106 7 0.2802067711101799 1106 8 0.21374985064094432 1106 9 0.1512216979841554 1106 10-11 0.19799811165597703 1106 12-13 0.15550635572685678 1106 14-15 0.06528482294681481 1106 16-17 0.10725219488746518 1106 18-19 0.12356115276065793 1106 20-21 0.17393251716807612 1106 22-23 0.04725514015709109 1106 24-25 0.04639192311001494 1106 26-27 0.1325114929890745 1106 28-29 0.03796673218201363 1106 30-31 0.06325232767912325 1106 32-33 -0.04316372951568326 1106 34-35 0.03123051421111711 1106 36-37 0.07132761922535735 1106 38-39 0.009040506402619997 1106 40-41 0.005055844364029838 1106 42-43 0.1097391855143357 1106 44-45 0.08520293303760695 1106 46-47 0.020556232109505856 1106 48-49 0.016076584814648243 1106 50-51 0.0011635354856380786 1106 52-53 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-0.8154363474988102 2216 40-41 -0.6504146099902925 2216 42-43 -0.4744206472828054 2216 44-45 -0.721777926330148 2216 46-47 -0.5244163088372815 2216 48-49 -0.47631094305320687 2216 50-51 -0.32525481279491686 2216 52-53 -0.3363150258745691 2216 54-55 -0.6134492651605328 2216 56-57 -1.1414590908335285 2216 58-59 -1.0857743072905848 2216 60-61 -0.6734560756295664 2216 62-63 -0.5865502945262051 2216 64-65 -0.44546047669469857 2216 66-67 -0.49543109504573835 2216 68-69 -0.3567337418637351 2216 70-71 -0.19702985966230102 2216 72-73 -0.36913836927656263 2216 74-75 -0.3906528725450258 2216 76-77 -0.3461326257364732 2216 78-79 -0.32529475289454624 2216 80-81 -0.27706834535792524 2216 82-83 -0.30377212272983556 2216 84-85 -0.21577083071714753 2216 86-87 -0.1799670868516685 2216 88-89 -0.2040859537537365 2216 90-91 -0.20293809955483155 2216 92-93 -0.1696650381619378 2216 94-95 -0.19204886708375568 2216 96-97 -0.18376705084335399 2216 98-99 -0.2619811235662475 2216 100-101 -0.44952368714301727 2216 102-103 -0.43531819673850336 2216 104-105 -0.279501607351726 2216 106-107 -0.12015958604483501 2216 108-109 -0.23109431031373262 2216 110-111 -0.316862987037382 2216 112-113 -0.21812750484469134 2216 114-115 -0.3439539209973681 2216 116-117 -0.27989770811064574 2216 118-119 -0.2864460589569724 2216 120-121 -0.26664010629376733 2216 122-123 -0.2564954906091401 2216 124-125 -0.6197512034548538 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 147.0 3 141.0 4 366.0 5 861.0 6 2248.0 7 4519.0 8 7259.0 9 12068.0 10 19305.0 11 24331.0 12 26304.0 13 32860.0 14 44117.0 15 33630.0 16 17714.0 17 15491.0 18 14600.0 19 14246.0 20 14261.0 21 14691.0 22 16021.0 23 19279.0 24 24435.0 25 31581.0 26 40893.0 27 52169.0 28 65643.0 29 83057.0 30 104669.0 31 130517.0 32 163246.0 33 208506.0 34 279955.0 35 393865.0 36 642136.0 37 1032758.0 38 422782.0 39 1890.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.70537531222817 18.098560358793065 10.923546991836181 22.272517337142585 2 18.665692723387647 21.435076013930278 37.561214129454704 22.338017133227368 3 17.81276962253966 27.969667199494385 32.012528678741766 22.205034499224187 4 11.347117215165078 16.66741016522864 37.44902569705482 34.536446922551455 5 10.264618266530412 42.084668070104044 35.3917330855623 12.258980577803246 6 30.02598589778448 37.67145222215936 18.4973636537862 13.805198226269955 7 26.388309112310072 34.59336817558661 22.470910722603346 16.54741198949997 8 27.56135046918913 33.39136775739983 20.283255506894474 18.76402626651657 9 27.499719007387053 11.620035184511837 19.624773305627006 41.255472502474106 10-11 24.175071730996738 25.956191070989327 29.801727624826142 20.067009573187796 12-13 25.604993917849473 25.06881515321512 29.81747816419489 19.508712764740523 14-15 22.529850636538608 26.25879083208953 27.97087695364631 23.240481577725546 16-17 21.176707784644687 28.660870947832674 28.1924043743152 21.97001689320744 18-19 20.313186516042997 26.487535018159225 31.933259083164096 21.266019382633687 20-21 21.64151523179336 25.720207119592697 32.09734381608155 20.54093383253239 22-23 21.222157121100466 26.146667926045236 31.89532819563366 20.73584675722064 24-25 20.916781192458032 26.0750834784921 32.17045574764543 20.83767958140444 26-27 20.800468827763616 26.276472806270114 31.96315270970335 20.95990565626292 28-29 20.754540648047552 26.1945333908361 32.059765827829885 20.991160133286463 30-31 20.90761984677616 25.897276078793567 32.2572541576315 20.937849916798772 32-33 20.71594674822389 26.23957617092924 32.02786200633461 21.016615074512263 34-35 21.183687349293322 26.2947581148119 31.724690592987585 20.796863942907194 36-37 21.38123258437691 26.073049107540047 31.368794144188712 21.17692416389433 38-39 21.119130649976412 26.19760796159859 31.485639719869678 21.197621668555318 40-41 21.135116019477845 26.272411196944397 31.447151306684834 21.145321476892924 42-43 21.1763333683078 26.310227838296342 31.191246946068624 21.322191847327232 44-45 21.27440586783903 26.58958961291698 30.746237296947754 21.389767222296232 46-47 21.546872850246555 26.762623352229564 30.013960311937325 21.676543485586556 48-49 21.705066212781908 26.81293965360714 29.71576414160038 21.766229992010576 50-51 21.667040316971768 26.929104185128498 29.625555038939183 21.778300458960548 52-53 21.623391528941326 26.97388737007676 29.682314737805726 21.720406363176185 54-55 21.46220310584975 27.158769947417515 29.63102534913238 21.748001597600357 56-57 21.75830658566496 27.263518190802955 29.376921492389673 21.60125373114241 58-59 21.878417912832155 26.757669364921345 29.468198464837265 21.895714257409235 60-61 21.827003427572254 26.800509712119318 29.763167791537416 21.60931906877101 62-63 21.529080827386693 26.854017543724108 29.941041582917737 21.675860045971465 64-65 21.34578499659562 26.925743605693842 29.919456216062102 21.80901518164844 66-67 21.599404253367773 27.27437442117572 29.538443402574586 21.587777922881916 68-69 21.75305376870934 26.967108544022793 29.582871131994732 21.696966555273136 70-71 21.50418354904774 26.777517684054043 29.650244209107047 22.068054557791168 72-73 21.59169584667722 27.04631833418485 29.404702862340965 21.95728295679697 74-75 21.347983480204892 27.287281719129343 29.411096942955666 21.9536378577101 76-77 21.25638297965135 27.29199420863638 29.387818373286756 22.063804438425517 78-79 21.601758977908727 27.174700923288952 29.21469661182425 22.00884348697807 80-81 21.422972922455795 27.150365727529874 29.10974318077033 22.316918169243998 82-83 21.5487486715137 27.02500937370212 29.257539241513296 22.168702713270882 84-85 21.410110569517812 26.9271099490884 29.22535294632943 22.437426535064358 86-87 21.2569212662545 26.94373953671519 29.298156966561724 22.501182230468586 88-89 21.56692028650955 26.886831070343526 29.209447024505785 22.33680161864114 90-91 21.478850072373902 27.04825126305422 29.140141198894653 22.332757465677226 92-93 21.456040676489373 26.861832370975087 29.019605790747455 22.662521161788085 94-95 21.20674257187749 26.847247149504756 29.13009817185252 22.81591210676524 96-97 21.390507271976208 26.960299614267193 29.063717876108182 22.58547523764842 98-99 21.281539069062493 26.96512289646601 28.78353875579174 22.969799278679755 100-101 21.23478324583217 26.9081908168387 28.894200599224728 22.962825338104402 102-103 21.207634114763437 26.585856999911528 28.951069341796853 23.255439543528187 104-105 21.105238531075486 26.45636696570927 29.118716320324285 23.319678182890957 106-107 21.067938215126073 26.558842906187373 29.129125461942955 23.244093416743603 108-109 21.2224404422713 26.764999852336985 28.560544765632624 23.452014939759096 110-111 21.10699371666057 26.849861782482616 28.627561858943366 23.415582641913453 112-113 21.078088109663202 26.54893537739876 28.792422426894625 23.580554086043414 114-115 20.763583939880053 26.659692921740298 28.654052688458435 23.922670449921213 116-117 20.66089323471512 26.695290791403043 28.81248726403013 23.831328709851704 118-119 20.703154890133295 26.640884586558272 28.604948693810133 24.051011829498297 120-121 20.65556091268746 26.66577274980002 28.63680959408739 24.04185674342513 122-123 20.662528222611638 26.586603633778854 28.450610710024705 24.3002574335848 124-125 20.51464373933444 26.519049160724357 28.24762126934257 24.718685830598638 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 539.0 1 513.0 2 1825.5 3 3357.0 4 2463.5 5 2109.5 6 3472.0 7 8015.0 8 12226.5 9 13052.5 10 10216.5 11 6145.0 12 5413.0 13 5588.5 14 5893.0 15 6251.0 16 6779.5 17 7393.0 18 8024.5 19 8415.0 20 9176.0 21 10648.0 22 12540.0 23 15389.5 24 18608.0 25 22988.0 26 29565.0 27 36009.0 28 41558.0 29 47674.0 30 56421.0 31 66063.5 32 74672.0 33 83013.0 34 93196.0 35 100201.0 36 106827.0 37 115354.5 38 118745.5 39 121086.0 40 122505.5 41 125457.5 42 129741.5 43 134705.0 44 142309.0 45 146659.0 46 145801.0 47 143487.5 48 141940.5 49 139202.0 50 137517.5 51 131631.0 52 124556.0 53 123262.0 54 118552.0 55 107921.0 56 95882.0 57 85724.0 58 78405.0 59 71711.5 60 60916.0 61 48563.5 62 39267.5 63 30535.0 64 22924.0 65 17550.0 66 12861.0 67 9846.0 68 7303.5 69 4629.5 70 3310.5 71 2643.0 72 2129.0 73 1884.5 74 1463.0 75 1041.5 76 694.5 77 431.0 78 251.5 79 98.0 80 41.0 81 21.0 82 11.0 83 7.5 84 3.0 85 1.5 86 1.5 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06661580970357835 2 1.7445217655258076E-4 3 1.7445217655258076E-4 4 0.0 5 0.05998662699458027 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.73826092612673E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.246086975375577E-5 26-27 0.0 28-29 1.246086975375577E-5 30-31 0.0 32-33 0.0 34-35 1.6199130679882498E-4 36-37 0.0 38-39 0.0 40-41 4.984347901502308E-5 42-43 0.0027413913458262692 44-45 0.009607330580145698 46-47 0.011787982787052956 48-49 0.009532565361623163 50-51 0.008535695781322702 52-53 0.007177460978163322 54-55 0.007314530545454636 56-57 0.00542047834288376 58-59 0.0038005652748955092 60-61 0.0024921739507511535 62-63 0.0017943652445408305 64-65 0.0021183478581384804 66-67 0.00291584352237885 68-69 0.0014953043704506923 70-71 0.002953226131640117 72-73 0.0027663130853337803 74-75 0.0010342521895617288 76-77 8.59800013009148E-4 78-79 8.722608827629038E-5 80-81 2.741391345826269E-4 82-83 1.3706956729131345E-4 84-85 4.3613044138145186E-4 86-87 4.610521808889634E-4 88-89 0.0014828435006969364 90-91 0.00161991306798825 92-93 5.482782691652538E-4 94-95 0.0014080782821744018 96-97 0.0017071391562645402 98-99 0.0014330000216819132 100-101 0.0010716347988229962 102-103 0.0011464000173455307 104-105 8.348782735016365E-4 106-107 6.355043574415442E-4 108-109 0.0011588608870992864 110-111 0.0011214782778380193 112-113 0.01142661756419404 114-115 0.021121174232616027 116-117 0.019775400299210405 118-119 0.011763061047545447 120-121 0.006392426183676709 122-123 0.0065793392299830465 124-125 0.005781843565742677 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4012561.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.3325608588485 #Duplication Level Percentage of deduplicated Percentage of total 1 39.76129225979946 9.277327714782327 2 16.00916831973537 7.470697882395497 3 11.8319279016904 8.282075335310966 4 8.826584057188299 8.237872387603517 5 6.0540053819297865 7.062772450683654 6 4.295423252171198 6.013393466747844 7 2.9434088114562327 4.807408565804143 8 2.075261010257373 3.8736923055860455 9 1.4622132906240992 3.070546253289353 >10 6.225182960962669 24.22087452083828 >50 0.307714723545508 4.954473340844179 >100 0.19313716929224353 8.204967804900381 >500 0.011442436063724191 1.7920240605569995 >1k 0.0028066352457415725 1.115226370187921 >5k 1.0794750945159892E-4 0.12674057307885672 >10k+ 3.238425283547968E-4 1.489906967390042 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22182 0.5528140257556209 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18586 0.4631954504866094 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18386 0.4582111025851071 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5032 0.12540619320179805 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4634 0.11548734087780846 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4242 0.10571801899086394 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.2460869753755768E-5 0.0 20-21 0.0 0.0 0.0 6.230434876877884E-5 0.0 22-23 0.0 0.0 0.0 1.993739160600923E-4 0.0 24-25 0.0 0.0 0.0 3.613652228589173E-4 0.0 26-27 0.0 0.0 0.0 3.987478321201846E-4 0.0 28-29 0.0 0.0 0.0 6.105826179340326E-4 0.0 30-31 0.0 0.0 0.0 0.0023551043834598405 0.0 32-33 0.0 0.0 0.0 0.006691487057766847 0.0 34-35 0.0 0.0 0.0 0.012909461064890977 0.0 36-37 0.0 0.0 0.0 0.022566635124051694 0.0 38-39 0.0 0.0 0.0 0.04155700062877549 0.0 40-41 0.0 0.0 0.0 0.06458468793371615 0.0 42-43 0.0 0.0 0.0 0.08993009701285538 0.0 44-45 0.0 0.0 0.0 0.12018508877497439 0.0 46-47 0.0 0.0 0.0 0.1601844806845304 0.0 48-49 0.0 0.0 0.0 0.2045077943986397 0.0 50-51 0.0 0.0 0.0 0.2486691168059501 0.0 52-53 0.0 4.984347901502307E-5 0.0 0.29747834363141146 0.0 54-55 0.0 4.984347901502307E-5 0.0 0.35427498796903023 0.0 56-57 0.0 4.984347901502307E-5 0.0 0.42942649345393125 0.0 58-59 0.0 4.984347901502307E-5 0.0 0.5211135731020663 0.0 60-61 0.0 4.984347901502307E-5 0.0 0.6090499309543207 0.0 62-63 0.0 4.984347901502307E-5 0.0 0.6992666279715125 2.4921739507511536E-5 64-65 0.0 7.476521852253461E-5 0.0 0.799240186005895 2.4921739507511536E-5 66-67 0.0 7.476521852253461E-5 0.0 0.9259547705318374 2.4921739507511536E-5 68-69 0.0 7.476521852253461E-5 0.0 1.0893292338733294 2.4921739507511536E-5 70-71 0.0 7.476521852253461E-5 0.0 1.2529778363494037 2.4921739507511536E-5 72-73 0.0 7.476521852253461E-5 0.0 1.411343030049886 2.4921739507511536E-5 74-75 0.0 7.476521852253461E-5 0.0 1.5765492412451798 2.4921739507511536E-5 76-77 0.0 7.476521852253461E-5 0.0 1.775424722515122 2.4921739507511536E-5 78-79 0.0 7.476521852253461E-5 0.0 2.0321809437912597 2.4921739507511536E-5 80-81 0.0 7.476521852253461E-5 0.0 2.2986815652148342 2.4921739507511536E-5 82-83 0.0 7.476521852253461E-5 0.0 2.5618177518048943 2.4921739507511536E-5 84-85 0.0 7.476521852253461E-5 0.0 2.831271599360109 2.4921739507511536E-5 86-87 0.0 7.476521852253461E-5 0.0 3.129983569097143 2.4921739507511536E-5 88-89 0.0 7.476521852253461E-5 0.0 3.493492061553706 2.4921739507511536E-5 90-91 0.0 7.476521852253461E-5 0.0 3.881398936988123 2.4921739507511536E-5 92-93 0.0 7.476521852253461E-5 0.0 4.265530168887151 2.4921739507511536E-5 94-95 0.0 7.476521852253461E-5 0.0 4.66900067064401 2.4921739507511536E-5 96-97 0.0 7.476521852253461E-5 0.0 5.092097042263033 2.4921739507511536E-5 98-99 0.0 7.476521852253461E-5 0.0 5.574606840867965 2.4921739507511536E-5 100-101 0.0 7.476521852253461E-5 0.0 6.110411779409709 2.4921739507511536E-5 102-103 0.0 7.476521852253461E-5 0.0 6.639413083065902 2.4921739507511536E-5 104-105 0.0 7.476521852253461E-5 0.0 7.188850213118256 2.4921739507511536E-5 106-107 0.0 1.2460869753755767E-4 0.0 7.756019160830203 2.4921739507511536E-5 108-109 0.0 1.4953043704506923E-4 0.0 8.354464891624078 2.4921739507511536E-5 110-111 0.0 1.4953043704506923E-4 0.0 9.017096562519548 2.4921739507511536E-5 112-113 0.0 1.4953043704506923E-4 0.0 9.672513389827595 2.4921739507511536E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11430 0.0 82.71144 1 GTATCAA 18805 0.0 50.17814 2 GTACATG 30125 0.0 48.13139 1 TACATGG 30470 0.0 47.341232 2 ACATGGG 30710 0.0 46.06014 3 CATGGGA 21700 0.0 42.852875 4 ATGGGAT 8780 0.0 37.36076 5 ATCAACG 26590 0.0 36.852768 4 TCAACGC 26660 0.0 36.800667 5 ATGGGAG 8145 0.0 36.618896 5 CATGGGG 9355 0.0 36.40079 4 CAACGCA 27095 0.0 36.274666 6 AACGCAG 27545 0.0 35.746845 7 TATCAAC 27720 0.0 35.414906 3 TACCTGG 3805 0.0 33.16957 2 GAGTACA 18870 0.0 31.253464 1 ACGCAGA 31605 0.0 31.060661 8 AGTACAT 18710 0.0 30.418844 2 CGCAGAG 32285 0.0 30.38802 9 CAGAGTA 35460 0.0 27.650373 9 >>END_MODULE