FastQCFastQC Report
Thu 26 May 2016
SRR936234_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936234_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973900
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA138510.46575204277211746No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37550.12626517367766232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55000.056.2491151
GTACATG290450.052.212381
TACATGG290500.051.015562
ACATGGG294550.048.173083
CATGGGA206350.044.9774864
CATGGGG79800.041.6012464
ATGGGAT84450.040.8604745
GTATCAA80600.038.531022
ATGGGAG72000.038.010225
ATGGGGG51200.035.5571175
ATCAACG93550.033.5152054
TCAACGC93600.033.4973035
CAACGCA94600.033.4576576
AACGCAG98400.032.1655967
GAGTACA169800.031.9545141
AGTACAT166350.031.3297082
TACCTGG26650.030.8075332
ATGGGAC38850.030.32145
TGGGATA31350.028.6559286
TATCAAC109650.028.6483963
Produced by FastQC (version 0.11.5)