FastQCFastQC Report
Thu 26 May 2016
SRR936234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936234_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973900
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242540.8155620565587276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207730.6985103735835099No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205200.6900030263290629No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62080.20874945357947478No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53190.17885604761424392No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50060.1683311476512324No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33640.11311745519351692No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31660.10645953125525405No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111700.083.4701461
GTATCAA182850.050.9553452
GTACATG257850.045.7937851
TACATGG259850.045.2037772
ACATGGG257700.044.7030223
CATGGGA185000.040.7088784
ATGGGAT78150.038.0900345
CATGGGG72150.037.9569974
ATCAACG260050.037.5683484
TCAACGC262250.037.298595
CAACGCA263500.037.187636
AACGCAG269500.036.3597037
TATCAAC267800.036.3032883
ATGGGAG61850.033.978595
ACGCAGA305350.031.8765168
CGCAGAG307250.031.5825779
TACCTGG23850.030.7034442
CCGAGTA2601.8189894E-1229.7673654
GAGTACA166050.029.5277941
GTCGGGA8350.029.2326182