FastQCFastQC Report
Thu 26 May 2016
SRR936233_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936233_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7104920
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87650.056.065731
GTACATG500900.053.210381
TACATGG502700.052.262422
ACATGGG507450.049.7097973
CATGGGA335700.044.2526864
CATGGGG169150.041.5384564
GTATCAA132150.039.3021621
ATGGGAT147900.036.8065725
ATGGGAG127400.034.837095
TACCTGG68300.033.884282
TCAACGC149550.033.1781435
CAACGCA152400.032.7531056
ATCAACG152300.032.6181264
AACGCAG157850.031.5094077
GAGTACA291750.030.383991
ATGGGGA96750.029.5163565
AGTACAT300750.028.1493682
TATCAAC183000.027.5360533
ACGCAGA190500.025.6092828
CATGGGT65350.025.0356884
Produced by FastQC (version 0.11.5)