FastQCFastQC Report
Thu 26 May 2016
SRR936233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936233_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7104920
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221510.3117698721449362No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193040.27169904798365074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174390.24544963208593482No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG71520.1006626394104367No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157100.076.283031
GTATCAA261500.047.536461
GTACATG444100.046.5532461
TACATGG450100.045.628412
ACATGGG445250.045.0959633
CATGGGA299050.041.205184
CATGGGG150750.035.341994
ATGGGAT131800.035.2293365
TCAACGC352650.035.0942425
ATCAACG352900.035.0356454
CAACGCA358050.034.6779446
AACGCAG368100.033.811976
TATCAAC378300.032.966513
TACCTGG63200.030.8945622
ATGGGAG122050.029.752085
ACGCAGA426850.029.2836596
GAGTACA291150.029.0910191
CGCAGAG431250.028.9296977
AGTACAT291800.027.846632
CAGAGTA485200.025.8601119
Produced by FastQC (version 0.11.5)