FastQCFastQC Report
Thu 26 May 2016
SRR936232_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936232_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114056
Sequences flagged as poor quality0
Sequence length125
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85500.7674659083564919No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37550.33705666501504417No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG19220.17252274571475762No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16970.15232627444221836No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15940.14308077870412259No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12870.11552381567892457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28850.057.5336951
GTACATG134750.051.258671
TACATGG136000.047.5066952
GAACAAA23100.044.555161
ACATGGG137450.043.5428773
CATGGGA103750.042.9494484
ATGGGAT37100.042.6551675
ATGGGAG33650.041.3708725
GTATCAA42100.041.122051
GTCGGGA6150.037.7270972
TACATGA11450.037.4102752
TACCTGG8400.034.7040942
CGCAGAA3950.034.6413352
CATGGGG30500.033.5499954
CGGGAGT6950.033.384414
GTACAAG11050.033.3805351
GTACATA10750.033.2052421
TCGGGAG7350.032.3773
TCAACGC52350.032.1613045
ATCAACG52750.032.142994
Produced by FastQC (version 0.11.5)