##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936232_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1114056 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.33886088311539 31.0 27.0 31.0 10.0 34.0 2 27.644182159604185 31.0 27.0 33.0 12.0 34.0 3 27.885767860861574 31.0 27.0 34.0 12.0 34.0 4 30.719625404827045 35.0 31.0 37.0 10.0 37.0 5 31.511126011618806 35.0 32.0 37.0 16.0 37.0 6 31.02594214294434 35.0 32.0 37.0 8.0 37.0 7 31.06446444343911 35.0 31.0 37.0 8.0 37.0 8 30.7773199910956 35.0 31.0 37.0 8.0 37.0 9 31.669728451711585 38.0 31.0 39.0 8.0 39.0 10-11 31.82555679427246 37.0 31.0 39.0 8.0 39.0 12-13 31.778220753714358 37.0 31.0 39.0 8.0 39.0 14-15 32.70862326489872 38.0 31.0 41.0 8.0 41.0 16-17 32.81766939902482 38.0 31.0 41.0 8.0 41.0 18-19 32.869574779005724 38.5 31.0 41.0 8.0 41.0 20-21 33.01041015891481 39.0 31.0 41.0 8.0 41.0 22-23 32.96268724372922 38.0 30.5 41.0 9.0 41.0 24-25 32.98931696431777 38.0 30.0 41.0 10.0 41.0 26-27 33.08497687728445 38.0 31.0 40.5 9.5 41.0 28-29 32.95929737822874 38.0 30.0 40.0 8.0 41.0 30-31 32.85613425177908 38.0 30.0 40.0 8.0 41.0 32-33 32.676296344169415 38.0 30.0 40.0 4.0 41.0 34-35 32.59697941575648 38.0 30.0 40.0 2.0 41.0 36-37 32.449470224118 38.0 30.0 40.0 2.0 41.0 38-39 32.178987411763856 38.0 30.0 40.0 2.0 41.0 40-41 32.245270435238446 38.0 30.0 40.0 2.0 41.0 42-43 32.118766022533876 38.0 30.0 40.0 2.0 41.0 44-45 32.00249493741787 38.0 30.0 40.0 2.0 41.0 46-47 31.959805880494336 38.0 30.0 40.0 2.0 41.0 48-49 31.841053322274643 37.5 30.0 40.0 2.0 41.0 50-51 31.690561336234445 37.0 30.0 40.0 2.0 41.0 52-53 31.512437435820104 37.0 30.0 40.0 2.0 41.0 54-55 31.314211314332493 37.0 30.0 40.0 2.0 41.0 56-57 31.08866295769692 36.0 29.5 40.0 2.0 41.0 58-59 30.809144692905925 36.0 29.0 40.0 2.0 41.0 60-61 30.528848639565695 35.5 29.0 40.0 2.0 41.0 62-63 30.251452799500207 35.0 28.5 39.0 2.0 41.0 64-65 29.942897394744968 35.0 28.0 39.0 2.0 41.0 66-67 29.643403024623538 35.0 28.0 39.0 2.0 40.5 68-69 29.36489952031137 35.0 28.0 38.0 2.0 40.0 70-71 29.032423414980933 34.0 28.0 37.5 2.0 40.0 72-73 28.72958585564819 34.0 27.5 37.0 2.0 39.5 74-75 28.423028555117515 34.0 27.0 36.5 2.0 39.0 76-77 28.111118292078675 34.0 27.0 36.0 2.0 39.0 78-79 27.79143014354754 34.0 26.0 36.0 2.0 38.5 80-81 27.510941550514517 34.0 26.0 35.0 2.0 37.0 82-83 27.218018214524225 34.0 26.0 35.0 2.0 37.0 84-85 27.223035017988323 34.0 26.0 35.0 2.0 36.5 86-87 27.095002405624136 34.0 26.0 35.0 2.0 36.0 88-89 26.91700821143641 34.0 26.0 35.0 2.0 36.0 90-91 26.728609244059545 34.0 25.0 35.0 2.0 36.0 92-93 26.590142237015016 34.0 25.0 35.0 2.0 35.0 94-95 26.4469313032738 34.0 25.0 35.0 2.0 35.0 96-97 26.330302516211034 34.0 25.0 35.0 2.0 35.0 98-99 26.208653335200385 33.5 24.5 35.0 2.0 35.0 100-101 26.074077963764836 33.0 24.0 35.0 2.0 35.0 102-103 25.16100896184752 31.5 21.5 34.0 2.0 35.0 104-105 25.735229647342685 33.0 23.0 35.0 2.0 35.0 106-107 25.827287856265755 33.0 23.0 35.0 2.0 35.0 108-109 25.755542360527656 33.0 23.0 35.0 2.0 35.0 110-111 25.599674971455656 33.0 20.0 35.0 2.0 35.0 112-113 25.516565145737736 33.0 20.0 35.0 2.0 35.0 114-115 25.371293723116253 33.0 19.0 35.0 2.0 35.0 116-117 25.22310548123254 33.0 18.0 35.0 2.0 35.0 118-119 25.005691814415073 33.0 12.5 35.0 2.0 35.0 120-121 24.771722426879798 33.0 2.0 35.0 2.0 35.0 122-123 24.446072280029014 33.0 2.0 35.0 2.0 35.0 124-125 22.60513250680397 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5373715113820197 1101 2 0.6329468957828688 1101 3 0.47927491132107036 1101 4 0.6415578153182828 1101 5 0.5267769522636279 1101 6 0.4388252408235864 1101 7 0.36827388779589043 1101 8 0.5482860532312728 1101 9 0.5449880892709764 1101 10-11 0.63436194544472 1101 12-13 0.6070976568675732 1101 14-15 0.5174822716279195 1101 16-17 0.4734168297924839 1101 18-19 0.49624150221995933 1101 20-21 0.46114110816652243 1101 22-23 0.4817140363487553 1101 24-25 0.5998094551135438 1101 26-27 0.5177494584917568 1101 28-29 0.48184595056014246 1101 30-31 0.5351136816690101 1101 32-33 0.6483553443907226 1101 34-35 0.6786655656667406 1101 36-37 0.5733944441763015 1101 38-39 0.8494442624517333 1101 40-41 0.6469599774346051 1101 42-43 0.6615559431786551 1101 44-45 0.5371446713010783 1101 46-47 0.5015432311598929 1101 48-49 0.637413421444684 1101 50-51 0.6138077078820956 1101 52-53 0.4282396430891815 1101 54-55 0.5104791976749254 1101 56-57 0.6846822434430635 1101 58-59 0.6748554540495562 1101 60-61 0.5095399606022291 1101 62-63 0.5314076683068834 1101 64-65 0.5978635425784908 1101 66-67 0.6764978142259679 1101 68-69 0.49399009207042965 1101 70-71 0.3942800458341864 1101 72-73 0.3302957927974326 1101 74-75 0.43232832580046576 1101 76-77 0.5703867711794679 1101 78-79 0.5238777639108463 1101 80-81 0.5266401534368441 1101 82-83 0.5357410616709579 1101 84-85 0.28491246154144534 1101 86-87 0.4364896080259193 1101 88-89 0.38225916544019967 1101 90-91 0.3554368463793267 1101 92-93 0.4626757781290003 1101 94-95 0.451089681650636 1101 96-97 0.5386930555854264 1101 98-99 0.4973751863340361 1101 100-101 0.41819244352404894 1101 102-103 0.642316438775282 1101 104-105 0.5762025400925346 1101 106-107 0.5554378500127513 1101 108-109 0.43668597701225664 1101 110-111 0.5059263719355407 1101 112-113 0.5748707748049604 1101 114-115 0.5917330880652685 1101 116-117 0.6312293634974537 1101 118-119 0.6334320942924307 1101 120-121 0.586024020294083 1101 122-123 0.5779717737125978 1101 124-125 0.27342919824480916 1102 1 0.5193667289085546 1102 2 0.43694882530722623 1102 3 0.5020374981505 1102 4 0.7928124561943974 1102 5 0.4785108405852405 1102 6 0.6100388576383153 1102 7 0.46593922333077487 1102 8 0.6542368756960322 1102 9 0.4779644539310759 1102 10-11 0.5224356980795655 1102 12-13 0.3763376449597331 1102 14-15 0.5002478987583387 1102 16-17 0.6074307638386216 1102 18-19 0.49632284360609447 1102 20-21 0.30086357402397823 1102 22-23 0.4095902294251985 1102 24-25 0.4133399946369565 1102 26-27 0.1572987738739755 1102 28-29 0.17649255390720242 1102 30-31 0.1770237307638709 1102 32-33 0.19674313478197547 1102 34-35 -0.06763681826927126 1102 36-37 0.15496218604941703 1102 38-39 0.3365061482450997 1102 40-41 0.3362470501881347 1102 42-43 0.3949193794921797 1102 44-45 0.48381039120734215 1102 46-47 0.45958841096603464 1102 48-49 0.45162802869079854 1102 50-51 0.34926518483897695 1102 52-53 0.3531893797797814 1102 54-55 0.23250267799947721 1102 56-57 0.2845779664529786 1102 58-59 0.3318734988359928 1102 60-61 0.3598283058150713 1102 62-63 0.38586059247970894 1102 64-65 0.3267800386449373 1102 66-67 0.3899374447493926 1102 68-69 0.27961420219066113 1102 70-71 0.1616061777879132 1102 72-73 0.15471324345838156 1102 74-75 0.059896121678924885 1102 76-77 0.1508430696863492 1102 78-79 0.019579171683552943 1102 80-81 0.09064498991565628 1102 82-83 -0.01165529723524017 1102 84-85 0.008753455133145849 1102 86-87 0.03972032786718316 1102 88-89 0.14525253511713032 1102 90-91 0.04084086804414255 1102 92-93 0.12686136583551288 1102 94-95 0.09976790079433329 1102 96-97 0.1167547836965106 1102 98-99 0.09746726211620427 1102 100-101 0.12595284513714233 1102 102-103 0.11085315724374567 1102 104-105 -0.07470156608064116 1102 106-107 -0.055740323293072436 1102 108-109 -0.10031618620189775 1102 110-111 0.07007305579057643 1102 112-113 0.10154774915875464 1102 114-115 0.028962873814055712 1102 116-117 0.1114300700340678 1102 118-119 0.02876839925328767 1102 120-121 0.1669938696425035 1102 122-123 0.26152661230828755 1102 124-125 0.19511094473770996 1103 1 0.5238153991656205 1103 2 0.5570050872212384 1103 3 0.5455224980579629 1103 4 0.5372834276377745 1103 5 0.313017068260919 1103 6 0.5646730626611713 1103 7 0.4034241862238197 1103 8 0.4231049748206388 1103 9 0.46917815666828844 1103 10-11 0.5764489862553113 1103 12-13 0.6072358923270826 1103 14-15 0.5446975869118731 1103 16-17 0.6973850510334856 1103 18-19 0.5888820204054568 1103 20-21 0.5095948123412981 1103 22-23 0.6035804761262895 1103 24-25 0.7690531699187346 1103 26-27 0.6004897405054734 1103 28-29 0.6659168409169922 1103 30-31 0.7423622738764095 1103 32-33 0.834391655130652 1103 34-35 0.693287061581934 1103 36-37 0.7275520848149881 1103 38-39 0.8986753318921288 1103 40-41 0.8776603994376728 1103 42-43 0.8936539491070405 1103 44-45 0.8655007471009668 1103 46-47 0.8741105548410744 1103 48-49 0.8115504372189193 1103 50-51 0.6289819782800308 1103 52-53 0.7238332455655048 1103 54-55 0.6507783151101307 1103 56-57 0.5368312190948785 1103 58-59 0.6951931368349946 1103 60-61 0.6179471868692445 1103 62-63 0.6371803877899822 1103 64-65 0.6546953866729943 1103 66-67 0.6082866480140616 1103 68-69 0.46851727993944436 1103 70-71 0.49470007411053274 1103 72-73 0.5428244440210008 1103 74-75 0.46311383122679217 1103 76-77 0.617871320940214 1103 78-79 0.5954402195616986 1103 80-81 0.49746929173121046 1103 82-83 0.4894701723851753 1103 84-85 0.4751570880981859 1103 86-87 0.4699643454120199 1103 88-89 0.3528802811242002 1103 90-91 0.45753645222417916 1103 92-93 0.42778973368479356 1103 94-95 0.6658386353454411 1103 96-97 0.5563125539480218 1103 98-99 0.23686008687144877 1103 100-101 0.48871495347834326 1103 102-103 0.5211625977708323 1103 104-105 0.5670150700491305 1103 106-107 0.6377259380657279 1103 108-109 0.6171446710519994 1103 110-111 0.5425077253443646 1103 112-113 0.6655826907685132 1103 114-115 0.6283604641755041 1103 116-117 0.44855622442120335 1103 118-119 0.5809228696695143 1103 120-121 0.594362329657681 1103 122-123 0.689121785628295 1103 124-125 0.532668548424347 1104 1 0.1535727781612728 1104 2 0.12424865597428436 1104 3 0.23529486532655852 1104 4 0.24852090524188597 1104 5 0.08884621526047098 1104 6 0.06773793818928908 1104 7 -0.27700979482652954 1104 8 -0.07209305407504729 1104 9 -0.08051009790339947 1104 10-11 0.10931403589231437 1104 12-13 0.16341961980141306 1104 14-15 -0.03268303269215522 1104 16-17 0.01301198945910187 1104 18-19 -0.016824571506205643 1104 20-21 -0.03351876841833956 1104 22-23 0.0031121258745869795 1104 24-25 0.10820810287084015 1104 26-27 -0.0761703837667369 1104 28-29 0.04974391538182488 1104 30-31 0.12640332390742515 1104 32-33 0.21564170504719016 1104 34-35 0.14636551440880652 1104 36-37 0.28603363699307494 1104 38-39 0.3243008526880189 1104 40-41 0.15899384325294363 1104 42-43 0.21708151155596056 1104 44-45 0.27314549841010916 1104 46-47 0.15512569752250727 1104 48-49 0.1463877558887674 1104 50-51 0.10556296819821398 1104 52-53 0.1505198256036806 1104 54-55 0.12113171755457586 1104 56-57 0.01140306829635307 1104 58-59 0.11508507751018726 1104 60-61 0.023287291547553934 1104 62-63 -0.08707049806220368 1104 64-65 0.04891691494439243 1104 66-67 -0.09210013861203237 1104 68-69 -0.10998479791970439 1104 70-71 -0.20081752047132895 1104 72-73 -0.04128143095604031 1104 74-75 -0.014063494950505628 1104 76-77 -0.07184963229264696 1104 78-79 -0.16831078875240024 1104 80-81 -0.0013362054774539445 1104 82-83 -0.015414826563247885 1104 84-85 0.028322695203108594 1104 86-87 0.022417643497618656 1104 88-89 0.035060342727867067 1104 90-91 -0.06850117952220813 1104 92-93 -0.02916407288395817 1104 94-95 0.053459865981150045 1104 96-97 0.13019602079146253 1104 98-99 0.1234086110723176 1104 100-101 0.06913682144842781 1104 102-103 0.05341866562537945 1104 104-105 0.0337610041805938 1104 106-107 0.04546370052688786 1104 108-109 -0.0692734711475751 1104 110-111 -0.008802983151028343 1104 112-113 0.0516571742321581 1104 114-115 -0.01154627841754774 1104 116-117 -0.001591961145862797 1104 118-119 0.052782753277455186 1104 120-121 0.14041466348884768 1104 122-123 0.3061467864935139 1104 124-125 0.36664680344861367 1105 1 0.36216649941916756 1105 2 0.5499723479693017 1105 3 0.3264781177718419 1105 4 0.34645560019898625 1105 5 0.35802891287439564 1105 6 0.2962121221248353 1105 7 0.44528517858034533 1105 8 0.37081748384290236 1105 9 0.4515731745277485 1105 10-11 0.5247305919407204 1105 12-13 0.42682530990523304 1105 14-15 0.3654228844152456 1105 16-17 0.32946174490574975 1105 18-19 0.25794074377820664 1105 20-21 0.09202823265851379 1105 22-23 0.2033366436535502 1105 24-25 0.31064349435010286 1105 26-27 0.2344645799554499 1105 28-29 0.3546336898796625 1105 30-31 0.3224626292148187 1105 32-33 0.5736794514773251 1105 34-35 0.36581125975712325 1105 36-37 0.45301152626743146 1105 38-39 0.4257201470976497 1105 40-41 0.20199575931034275 1105 42-43 0.49187864512614254 1105 44-45 0.46688554898129553 1105 46-47 0.3867255308742372 1105 48-49 0.3698724348870073 1105 50-51 0.4270047143857383 1105 52-53 0.4349449735835691 1105 54-55 0.20525081339821583 1105 56-57 0.31584589531126994 1105 58-59 0.5447249044584836 1105 60-61 0.5201840143635508 1105 62-63 0.4337665018314034 1105 64-65 0.43263201799089046 1105 66-67 0.28322488020550196 1105 68-69 0.38948224579364776 1105 70-71 0.37847364766743397 1105 72-73 0.3930953432862623 1105 74-75 0.4864595181218405 1105 76-77 0.387790860851009 1105 78-79 0.3488964407598587 1105 80-81 0.46987620047216794 1105 82-83 0.46367459375730036 1105 84-85 0.4995738796885121 1105 86-87 0.5339830932142853 1105 88-89 0.4731354955302116 1105 90-91 0.4326939948370274 1105 92-93 0.5161327370345994 1105 94-95 0.5271918824351829 1105 96-97 0.4466457762381033 1105 98-99 0.3762968662470172 1105 100-101 0.4205598445634209 1105 102-103 0.41550031731259196 1105 104-105 0.5277080724735725 1105 106-107 0.4801173932875358 1105 108-109 0.5201657415089436 1105 110-111 0.48688315332356424 1105 112-113 0.6012608874261076 1105 114-115 0.5326920763384422 1105 116-117 0.6056928353811699 1105 118-119 0.6179823981058377 1105 120-121 0.5565521025742299 1105 122-123 0.4339305136278533 1105 124-125 0.5410661943074153 1106 1 -0.6151937136338148 1106 2 -0.8197904871549788 1106 3 -0.455022401680484 1106 4 -0.5349105981852844 1106 5 -0.5167588482085463 1106 6 -0.4794949532810371 1106 7 -0.6609622047754726 1106 8 -0.5490221061774072 1106 9 -0.6248066691713738 1106 10-11 -0.4959694863647748 1106 12-13 -0.5822529917182528 1106 14-15 -0.8023931674369464 1106 16-17 -0.5525033742581655 1106 18-19 -0.6797976615806789 1106 20-21 -0.6716907358797286 1106 22-23 -0.8343217117758925 1106 24-25 -4.508321555036638 1106 26-27 -1.7897752364530461 1106 28-29 -1.32689696943714 1106 30-31 -1.204541138752809 1106 32-33 -5.573857607957322 1106 34-35 -1.7007052890636416 1106 36-37 -0.9339639819430161 1106 38-39 -0.5534993370164152 1106 40-41 -1.2334913629237683 1106 42-43 -0.8083870896275442 1106 44-45 -0.8803126065752913 1106 46-47 -0.9704827423731928 1106 48-49 -0.6813139709680698 1106 50-51 -0.5963522268928898 1106 52-53 -0.7019864393267632 1106 54-55 -0.7365831246706662 1106 56-57 -0.47069747895800873 1106 58-59 -0.31616023842967067 1106 60-61 -0.25910786357290405 1106 62-63 -0.5858341013535409 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-0.8076084240805059 2216 60-61 -0.9383736544334056 2216 62-63 -0.836076579207436 2216 64-65 -0.7724300128623263 2216 66-67 -0.8467771813634997 2216 68-69 -0.7610271535226794 2216 70-71 -0.8845181524019274 2216 72-73 -1.0670196890675854 2216 74-75 -0.8367030885323956 2216 76-77 -0.7642444191468165 2216 78-79 -0.7934035990332191 2216 80-81 -0.8829199423162564 2216 82-83 -0.9413487627007662 2216 84-85 -1.0488819412074903 2216 86-87 -0.8406209337171546 2216 88-89 -0.8058793301873024 2216 90-91 -0.7998231809093355 2216 92-93 -0.795136694052264 2216 94-95 -0.7988263968099325 2216 96-97 -0.8589124694785468 2216 98-99 -0.8015593257314038 2216 100-101 -0.813224317153086 2216 102-103 -0.8528518817742459 2216 104-105 -0.7911794154007268 2216 106-107 -0.936964863315481 2216 108-109 -0.9950337752209286 2216 110-111 -0.8667054949424013 2216 112-113 -0.9003672726475322 2216 114-115 -1.0247831888288736 2216 116-117 -0.9223981996702975 2216 118-119 -0.9402000331711982 2216 120-121 -1.0678971468708234 2216 122-123 -0.9394181935766142 2216 124-125 -1.1360072406980244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 65902.0 3 12065.0 4 11142.0 5 9829.0 6 9616.0 7 7380.0 8 6542.0 9 6929.0 10 8755.0 11 10029.0 12 9649.0 13 8964.0 14 8578.0 15 8014.0 16 7490.0 17 6602.0 18 5923.0 19 5382.0 20 4914.0 21 5038.0 22 6266.0 23 10212.0 24 16269.0 25 20985.0 26 24091.0 27 18932.0 28 20254.0 29 23917.0 30 27439.0 31 33111.0 32 42006.0 33 53308.0 34 72292.0 35 103023.0 36 159941.0 37 199140.0 38 63966.0 39 161.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.43013995705782 20.028436631551735 9.341720703447583 20.19970270794287 2 17.744170849580275 24.763746167158562 36.149978098048926 21.342104885212233 3 16.695211012731857 35.020322138204904 27.107344693624018 21.177122155439225 4 10.07022986277171 20.022602095406334 33.532335896938754 36.3748321448832 5 10.227260486943159 48.31650587225731 30.06762700484358 11.388606635955952 6 26.95030941049536 40.09706890330271 19.15831728084994 13.794304405351983 7 27.155799589427076 39.37523618714729 19.131585301596964 14.337378921828675 8 26.015887976302682 39.07795879897671 17.945155064853463 16.960998159867152 9 27.299117541719514 20.315031592024393 16.497598395758885 35.88825247049721 10-11 22.5119418264857 33.62099337864263 25.11308998322781 18.753974811643857 12-13 23.538749746419807 32.32465266245739 24.98047656919014 19.156121021932666 14-15 21.7436088470791 32.402606728663244 22.985934077770995 22.867850346486662 16-17 21.16110575772985 34.487898341309545 23.64220722477574 20.708788676184863 18-19 20.926131633218663 32.61585621733462 25.81423060610345 20.643781543343277 20-21 23.108122785647538 31.154250434679575 25.348259800599443 20.389366979073444 22-23 22.777206038584087 31.44692341175419 25.2186397059319 20.55723084372983 24-25 21.848692351227488 31.283304975622546 25.732299918809126 21.13570275434084 26-27 21.57242301702355 31.307442697557104 25.526078390011175 21.594055895408175 28-29 21.81450970447849 30.908556412540754 25.803702265836957 21.473231617143792 30-31 21.739185072849033 30.93778064818426 25.974450888895973 21.34858339007074 32-33 21.08442657163759 31.2810972626779 25.89168301100943 21.74279315467508 34-35 21.613706745657733 31.0535882590548 26.081818589829904 21.250886405457564 36-37 21.909819371250737 30.751858304766472 25.291256340587026 22.047065983395765 38-39 21.765869890216027 30.873842008201596 25.843778317250145 21.516509784332232 40-41 21.57639102698171 30.897418520337038 26.148309188717572 21.377881263963676 42-43 21.35919454207376 31.01967192660649 25.90203577130101 21.719097760018744 44-45 21.469384494343394 30.810424832245186 26.176348220885657 21.54384245252576 46-47 21.767230062245943 30.99537407931522 25.705792880651067 21.531602977787774 48-49 21.529162859250988 30.96124604372858 25.51361962705578 21.99597146996465 50-51 21.692547496810057 31.086393455940204 25.69349539090982 21.527563656339918 52-53 21.57393930499248 30.92809045950098 25.892569019847034 21.605401215659505 54-55 21.318749873771093 31.384523550387218 25.745984798673305 21.55074177716839 56-57 21.679836344720922 31.489458722192648 25.36764540877466 21.463059524311767 58-59 21.695123533004892 30.764428490706692 25.545249357190336 21.995198619098076 60-61 21.310145895409683 31.283158812676078 25.83453278416662 21.57216250774762 62-63 21.235726060646318 31.37015006963335 25.801277232295334 21.592846637424998 64-65 20.983500313944194 31.654820266388047 25.617038350560996 21.74464106910677 66-67 21.073064330451214 31.831848140011353 25.35365254330474 21.741434986232697 68-69 21.218646647316945 31.40902764718634 25.539930461274146 21.83239524422257 70-71 21.33669343075521 31.192041685554123 25.64657938791173 21.824685495778933 72-73 21.11581464486525 31.62157019036745 25.282930122004636 21.97968504276266 74-75 20.950087742526176 31.704449062209676 25.554303871891427 21.791159323372725 76-77 20.951299531890257 31.75646624268999 25.85536620723393 21.436868018185816 78-79 21.077486230494696 31.597424186934948 25.591711727238124 21.73337785533223 80-81 20.799332169417127 31.969067954454673 25.373208683592818 21.85839119253538 82-83 21.03660903788007 31.499193711623878 25.300041155938818 22.164156094557228 84-85 20.747988205250188 31.296345333040108 25.899663840654185 22.056002621055512 86-87 20.71540453568271 31.814407726728035 25.57068546885028 21.899502268738978 88-89 20.901975216663452 31.523219230648397 25.646758912261962 21.928046640426192 90-91 20.85520912342748 31.622541077415388 25.43815161728999 22.084098181867144 92-93 20.736435152272417 31.524492485117445 25.528788498962353 22.21028386364779 94-95 20.534793823108455 31.29695962185008 25.917066070765777 22.251180484275693 96-97 20.74013401492745 31.702878224145124 25.5707752310254 21.986212529902023 98-99 20.575375542500147 31.814632132165826 25.344439906467812 22.265552418866214 100-101 20.420230787632025 31.79193657042811 25.56261834587087 22.225214296068998 102-103 20.358339740111457 31.74306104414102 25.621996051360146 22.276603164387378 104-105 19.743423683491248 32.36928713990204 25.6426413609029 22.24464781570381 106-107 19.753746338262626 32.60652867521293 25.52738667632508 22.112338310199366 108-109 20.06979009115349 32.89092549290654 24.899578566587827 22.139705849352143 110-111 20.04854344966377 33.03773113230199 24.944134191041993 21.96959122699224 112-113 19.73879207040945 33.00761632055868 25.361360076477375 21.892231532554497 114-115 19.514431513704437 33.26195744375278 25.043871263088448 22.179739779454337 116-117 19.601518418317244 33.34892204507142 25.12907812368162 21.920481412929714 118-119 19.424911180247996 33.33159792972333 24.900453658440256 22.343037231588415 120-121 19.406537379213773 33.22273137322664 25.0338627805629 22.33686846699669 122-123 19.49059965621087 33.015919321757 25.00599162518906 22.487489396843063 124-125 19.77272217260369 33.16918823576933 24.394980499167456 22.66310909245953 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6966.0 1 8028.5 2 8367.5 3 6285.5 4 4029.0 5 2712.5 6 2138.5 7 1913.0 8 1808.5 9 1783.0 10 1617.0 11 1443.0 12 1404.0 13 1392.5 14 1452.0 15 1470.5 16 1538.5 17 1649.0 18 1817.0 19 2055.5 20 2395.5 21 2924.5 22 3614.5 23 4568.0 24 5599.0 25 6786.5 26 8168.0 27 9381.5 28 10466.0 29 12346.0 30 15457.0 31 18713.5 32 21294.0 33 22934.5 34 24405.5 35 26173.0 36 28892.0 37 32094.0 38 33354.0 39 32880.0 40 31954.0 41 31848.5 42 31305.0 43 32003.5 44 34586.0 45 36119.0 46 36292.5 47 35932.0 48 35619.0 49 34034.5 50 33389.0 51 32740.0 52 31917.5 53 32269.5 54 31634.0 55 29996.0 56 28042.0 57 26035.5 58 24777.5 59 23832.5 60 21203.5 61 18144.0 62 15350.5 63 11160.5 64 7687.5 65 6018.5 66 4770.0 67 3756.0 68 2844.0 69 2038.5 70 1483.0 71 1207.0 72 1111.5 73 1126.5 74 889.5 75 626.5 76 422.5 77 294.0 78 236.0 79 156.0 80 97.5 81 63.5 82 42.5 83 38.5 84 30.0 85 20.0 86 16.5 87 12.5 88 12.0 89 10.5 90 6.0 91 4.0 92 4.5 93 3.0 94 1.0 95 1.0 96 1.5 97 0.5 98 0.0 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 3.590483781784757E-4 6 1.7952418908923786E-4 7 2.6928628363385684E-4 8 5.385725672677137E-4 9 8.078588509015705E-4 10-11 8.527398981738799E-4 12-13 8.976209454461895E-4 14-15 0.001436193512713903 16-17 0.0013015503708969747 18-19 0.0012117882763523558 20-21 0.001525955607258522 22-23 0.08801173370099888 24-25 0.0013015503708969747 26-27 9.873830399908083E-4 28-29 7.62977803629261E-4 30-31 4.936915199954041E-4 32-33 9.873830399908083E-4 34-35 5.385725672677137E-4 36-37 2.6928628363385684E-4 38-39 4.0392942545078525E-4 40-41 4.488104727230947E-4 42-43 5.834536145400232E-4 44-45 4.0392942545078525E-4 46-47 6.732157090846421E-4 48-49 1.7952418908923786E-4 50-51 6.732157090846421E-4 52-53 9.425019927184989E-4 54-55 7.62977803629261E-4 56-57 6.283346618123326E-4 58-59 1.3464314181692842E-4 60-61 4.0392942545078525E-4 62-63 5.834536145400232E-4 64-65 4.0392942545078525E-4 66-67 3.141673309061663E-4 68-69 4.4881047272309466E-5 70-71 8.976209454461893E-5 72-73 0.0 74-75 8.976209454461893E-5 76-77 8.976209454461893E-5 78-79 0.0 80-81 8.976209454461893E-5 82-83 4.4881047272309466E-5 84-85 8.976209454461893E-5 86-87 8.976209454461893E-5 88-89 8.976209454461893E-5 90-91 8.976209454461893E-5 92-93 0.0 94-95 4.4881047272309466E-5 96-97 8.976209454461893E-5 98-99 8.976209454461893E-5 100-101 1.3464314181692842E-4 102-103 1.3464314181692842E-4 104-105 1.3464314181692842E-4 106-107 1.3464314181692842E-4 108-109 8.976209454461893E-5 110-111 2.2440523636154736E-4 112-113 8.976209454461893E-5 114-115 8.976209454461893E-5 116-117 1.7952418908923786E-4 118-119 1.7952418908923786E-4 120-121 8.976209454461893E-5 122-123 8.976209454461893E-5 124-125 8.976209454461893E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1114056.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.00658391047136 #Duplication Level Percentage of deduplicated Percentage of total 1 65.94950370240234 23.746163389144918 2 10.123547450335153 7.290287214842621 3 6.577132890508379 7.10460261937233 4 4.540553895442789 6.539593393451136 5 3.1813970008571486 5.727561903194242 6 2.1685004860312245 4.684817682610873 7 1.5189395451291032 3.8284276930636976 8 1.093371167125012 3.14948485395014 9 0.8023571685068662 2.600112664260962 >10 3.7793999154402584 23.36194689698582 >50 0.17728853723532928 4.37142184473051 >100 0.0840078665297976 5.30921779959037 >500 0.002500234035443193 0.5934440524210676 >1k 0.0012501170177215966 0.923205212011233 >5k 2.500234035443193E-4 0.769712780370165 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8550 0.7674659083564919 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3755 0.33705666501504417 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1922 0.17252274571475762 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1697 0.15232627444221836 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1594 0.14308077870412259 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1287 0.11552381567892457 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.488104727230947E-5 0.0 12-13 0.0 0.0 0.0 8.976209454461895E-5 0.0 14-15 0.0 0.0 0.0 4.936915199954043E-4 0.0 16-17 0.0 0.0 0.0 5.385725672677137E-4 0.0 18-19 0.0 0.0 0.0 7.62977803629261E-4 0.0 20-21 0.0 0.0 0.0 8.976209454461895E-4 0.0 22-23 5.385725672677137E-4 0.0 0.0 0.0013913124654415937 0.0 24-25 5.385725672677137E-4 0.0 0.0 0.0021542902690708547 0.0 26-27 5.385725672677137E-4 0.0 0.0 0.0026031007417939493 0.0 28-29 7.62977803629261E-4 0.0 0.0 0.0037700079708739955 0.0 30-31 0.0011669072290800462 0.0 0.0 0.007046324421752587 0.0 32-33 0.0011669072290800462 0.0 0.0 0.014182410938049792 0.0 34-35 0.0011669072290800462 0.0 0.0 0.024684575999770208 0.0 36-37 0.0011669072290800462 0.0 0.0 0.04061734778144007 0.0 38-39 0.0011669072290800462 0.0 0.0 0.06866800232663349 0.0 40-41 0.0011669072290800462 0.0 0.0 0.10179021521359788 0.0 42-43 0.0011669072290800462 0.0 0.0 0.13729112360599466 0.0 44-45 0.0011669072290800462 0.0 0.0 0.17970371327832713 0.0 46-47 0.0011669072290800462 0.0 0.0 0.24002384081231107 0.0 48-49 0.0011669072290800462 0.0 0.0 0.3016903997644642 0.0 50-51 0.0011669072290800462 0.0 0.0 0.36802458763293766 0.0 52-53 0.0011669072290800462 0.0 0.0 0.4388019991813697 0.0 54-55 0.0011669072290800462 0.0 0.0 0.516715497246099 0.0 56-57 0.0011669072290800462 0.0 0.0 0.6251032264087264 0.0 58-59 0.0011669072290800462 0.0 0.0 0.7635163761965287 0.0 60-61 0.0011669072290800462 0.0 0.0 0.886535326769929 0.0 62-63 0.0011669072290800462 0.0 0.0 1.0041685516706522 0.0 64-65 0.0011669072290800462 0.0 0.0 1.1310921533567433 0.0 66-67 0.0014361935127139031 0.0 0.0 1.3004283447151668 0.0 68-69 0.0014361935127139031 0.0 0.0 1.5302641877966638 0.0 70-71 0.0014361935127139031 0.0 0.0 1.7526048959836849 0.0 72-73 0.0014361935127139031 0.0 0.0 1.9562750885054252 0.0 74-75 0.0014361935127139031 0.0 0.0 2.1687419662925382 0.0 76-77 0.0014361935127139031 0.0 0.0 2.422678931759265 0.0 78-79 0.0014361935127139031 0.0 0.0 2.7662433486287945 0.0 80-81 0.0018850039854369977 0.0 0.0 3.1112888400583096 0.0 82-83 0.0018850039854369977 0.0 0.0 3.4492880070660723 0.0 84-85 0.0018850039854369977 0.0 0.0 3.781003827455711 0.0 86-87 0.0018850039854369977 0.0 0.0 4.135833387190591 0.0 88-89 0.0018850039854369977 0.0 0.0 4.574276337993782 0.0 90-91 0.002064528174526236 0.0 0.0 5.062582132316509 0.0 92-93 0.002064528174526236 0.0 0.0 5.541821955090229 0.0 94-95 0.002064528174526236 0.0 0.0 6.041572416467394 0.0 96-97 0.002064528174526236 0.0 0.0 6.534545839706442 0.0 98-99 0.0021542902690708547 0.0 0.0 7.110190152021083 0.0 100-101 0.0023786955054324018 0.0 0.0 7.74597506768062 0.0 102-103 0.002827505978155497 0.0 0.0 8.38301665266378 0.0 104-105 0.004039294254507852 0.0 0.0 9.024097531901448 0.0 106-107 0.004039294254507852 0.0 0.0 9.657189584724645 0.0 108-109 0.004129056349052472 0.0 0.0 10.333995777591074 0.0 110-111 0.0042188184435970905 0.0 0.0 11.076014132144165 0.0 112-113 0.0042188184435970905 0.0 0.0 11.813319976733665 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2885 0.0 57.533695 1 GTACATG 13475 0.0 51.25867 1 TACATGG 13600 0.0 47.506695 2 GAACAAA 2310 0.0 44.55516 1 ACATGGG 13745 0.0 43.542877 3 CATGGGA 10375 0.0 42.949448 4 ATGGGAT 3710 0.0 42.655167 5 ATGGGAG 3365 0.0 41.370872 5 GTATCAA 4210 0.0 41.12205 1 GTCGGGA 615 0.0 37.727097 2 TACATGA 1145 0.0 37.410275 2 TACCTGG 840 0.0 34.704094 2 CGCAGAA 395 0.0 34.641335 2 CATGGGG 3050 0.0 33.549995 4 CGGGAGT 695 0.0 33.38441 4 GTACAAG 1105 0.0 33.380535 1 GTACATA 1075 0.0 33.205242 1 TCGGGAG 735 0.0 32.377 3 TCAACGC 5235 0.0 32.161304 5 ATCAACG 5275 0.0 32.14299 4 >>END_MODULE