FastQCFastQC Report
Thu 26 May 2016
SRR936232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936232_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114056
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173291.5554873363637016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158091.419048952655881No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157131.4104317915795974No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43390.3894777282291016No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36860.33086308049146546No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35160.31560352441888023No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26000.23338144581600923No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21470.19271921698729685No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG17540.15744271383126163No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11520.10340593291540102No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71000.082.806871
GTATCAA116900.050.2639432
CCGTTGC1800.049.5769929
GTACATG108150.043.0712781
ATGGGAG24750.042.0936625
TACATGG113500.040.9646342
TATAACG1750.040.8222662
ACATGGG111000.040.546443
CATGGGA83700.037.767914
GTTATCA6450.036.0174061
ATCAACG170800.035.9355854
TCAACGC171600.035.768055
CATGGGG25300.035.7665444
CAACGCA174250.035.268666
ATGGGAT31350.035.1308065
TATCAAC177300.034.6853033
TACCTGG8250.034.6370742
AACGCAG177950.034.535347
GTATAAC3050.033.201421
TATTCCG2700.033.0735935