##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936232_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1114056 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.554008057045607 31.0 31.0 34.0 30.0 34.0 2 31.256081381905396 31.0 31.0 34.0 28.0 34.0 3 31.59093259225748 31.0 31.0 34.0 30.0 34.0 4 35.48318486682896 37.0 35.0 37.0 33.0 37.0 5 35.14011234623753 37.0 35.0 37.0 33.0 37.0 6 35.37681498955169 37.0 35.0 37.0 33.0 37.0 7 35.19008739237525 37.0 35.0 37.0 32.0 37.0 8 35.27865116295769 37.0 35.0 37.0 32.0 37.0 9 36.88772557214359 39.0 37.0 39.0 33.0 39.0 10-11 36.78230672425803 39.0 37.0 39.0 32.0 39.0 12-13 36.717615631530194 39.0 37.0 39.0 32.0 39.0 14-15 37.65183886626884 39.5 36.5 41.0 32.0 41.0 16-17 37.60591657869982 39.0 36.5 41.0 32.0 41.0 18-19 37.8881384777785 40.0 37.0 41.0 32.0 41.0 20-21 37.978473703296785 40.0 37.0 41.0 33.0 41.0 22-23 37.825853906805406 40.0 37.0 41.0 32.0 41.0 24-25 37.75285892271124 40.0 37.0 41.0 32.0 41.0 26-27 37.507183660426406 40.0 36.0 41.0 32.0 41.0 28-29 37.29279048809037 39.5 36.0 41.0 32.0 41.0 30-31 36.8984665043768 39.0 35.5 41.0 31.0 41.0 32-33 36.41226518236067 38.0 35.0 40.5 30.5 41.0 34-35 36.046516961445384 38.0 35.0 40.0 30.0 41.0 36-37 35.7083584667198 38.0 35.0 40.0 29.5 41.0 38-39 35.579953341663256 38.0 35.0 40.0 29.0 41.0 40-41 35.45300146491738 38.0 35.0 41.0 27.5 41.0 42-43 35.18352578326403 38.0 35.0 41.0 26.5 41.0 44-45 34.75172836913046 38.0 34.5 41.0 23.5 41.0 46-47 34.04689396224247 38.0 33.5 41.0 18.0 41.0 48-49 33.47387474238279 38.0 33.0 40.5 10.0 41.0 50-51 33.23217324802344 38.0 33.0 40.0 7.0 41.0 52-53 33.10717459445486 38.0 33.0 40.0 7.0 41.0 54-55 32.93050483997214 38.0 33.0 40.0 3.5 41.0 56-57 32.720978119591834 37.0 33.0 40.0 2.0 41.0 58-59 32.48907864595675 37.0 32.5 40.0 2.0 41.0 60-61 32.25386560460156 36.5 32.0 40.0 2.0 41.0 62-63 32.03235609341002 36.0 32.0 40.0 2.0 41.0 64-65 31.776664727805425 36.0 31.5 39.0 2.0 41.0 66-67 31.485187010347772 35.0 31.0 39.0 2.0 41.0 68-69 31.176777917806646 35.0 31.0 39.0 2.0 41.0 70-71 30.85971665697236 35.0 31.0 38.0 2.0 40.0 72-73 30.59574114766224 35.0 31.0 37.0 2.0 40.0 74-75 30.40047582886318 35.0 31.0 37.0 2.0 39.0 76-77 30.104749671470735 35.0 31.0 36.5 2.0 39.0 78-79 29.78697300674293 35.0 30.0 36.0 2.0 39.0 80-81 29.51829127081583 35.0 30.0 36.0 2.0 37.5 82-83 29.26297915006068 35.0 30.0 35.0 2.0 37.0 84-85 29.008274269875123 34.5 30.0 35.0 2.0 37.0 86-87 28.799897401925936 34.0 29.5 35.0 2.0 36.0 88-89 28.62181928017981 34.0 29.0 35.0 2.0 36.0 90-91 28.468384443869965 34.0 29.0 35.0 2.0 36.0 92-93 28.31402775084915 34.0 29.0 35.0 2.0 35.5 94-95 28.1719379456688 34.0 29.0 35.0 2.0 35.0 96-97 28.07638933769936 34.0 29.0 35.0 2.0 35.0 98-99 27.957017869837784 34.0 29.0 35.0 2.0 35.0 100-101 27.840378760134143 34.0 29.0 35.0 2.0 35.0 102-103 26.83279386314512 32.5 26.0 34.5 2.0 35.0 104-105 27.511525004129055 34.0 28.0 35.0 2.0 35.0 106-107 27.623294969014125 34.0 29.0 35.0 2.0 35.0 108-109 27.5595629842665 34.0 29.0 35.0 2.0 35.0 110-111 27.465653880953923 34.0 28.0 35.0 2.0 35.0 112-113 27.352905958048787 34.0 27.0 35.0 2.0 35.0 114-115 27.286777774187293 34.0 27.0 35.0 2.0 35.0 116-117 27.151136029068557 34.0 27.0 35.0 2.0 35.0 118-119 26.990785920994995 34.0 27.0 35.0 2.0 35.0 120-121 26.75576721457449 34.0 26.0 35.0 2.0 35.0 122-123 26.491938017478475 34.0 25.0 35.0 2.0 35.0 124-125 24.596228555835616 31.5 13.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19705345615182424 1101 2 0.6170079686412393 1101 3 0.3349199889693253 1101 4 0.19134741365236607 1101 5 0.4291604842205814 1101 6 0.3012509057973176 1101 7 0.41192184846555335 1101 8 0.2879113825117514 1101 9 0.2132464903980491 1101 10-11 0.271894567678288 1101 12-13 0.30613319503671477 1101 14-15 0.2776133951878279 1101 16-17 0.44751028510364677 1101 18-19 0.3827801665757846 1101 20-21 0.2524178119562279 1101 22-23 0.18688632818321338 1101 24-25 0.17015270738783528 1101 26-27 0.27368478027170795 1101 28-29 0.31885550386682127 1101 30-31 0.2337156210889475 1101 32-33 0.27638262529679025 1101 34-35 0.2407924195926725 1101 36-37 0.3051273943346473 1101 38-39 0.3071807804712634 1101 40-41 0.5116292256797905 1101 42-43 0.4513162504958643 1101 44-45 0.5239103655578035 1101 46-47 0.7637455992563247 1101 48-49 0.7776941080299835 1101 50-51 0.7750914805800875 1101 52-53 0.6304931760181844 1101 54-55 0.6566585758851176 1101 56-57 0.4099728077780327 1101 58-59 0.46721884663434565 1101 60-61 0.6554107794930673 1101 62-63 0.5478176838088729 1101 64-65 0.5243327487931388 1101 66-67 0.5159278106201199 1101 68-69 0.5404049405989682 1101 70-71 0.5226193597788082 1101 72-73 0.5071563958754375 1101 74-75 0.6291318017767225 1101 76-77 0.5725127910982408 1101 78-79 0.6172801298010029 1101 80-81 0.573698650843518 1101 82-83 0.5172281575476241 1101 84-85 0.46356046717463784 1101 86-87 0.42819842647906725 1101 88-89 0.5841831657700212 1101 90-91 0.6661713158696259 1101 92-93 0.5540796965517174 1101 94-95 0.641035394068183 1101 96-97 0.623718994083049 1101 98-99 0.6172042346596811 1101 100-101 0.5935646910752901 1101 102-103 0.6264397541240463 1101 104-105 0.5224836685020122 1101 106-107 0.5731593569155926 1101 108-109 0.5902651900245779 1101 110-111 0.6364620996775194 1101 112-113 0.7289654870331077 1101 114-115 0.4795358694589389 1101 116-117 0.49720495294070943 1101 118-119 0.488622391884455 1101 120-121 0.5193089839478269 1101 122-123 0.4013681290716917 1101 124-125 0.5122832772100168 1102 1 0.09175869863749497 1102 2 0.5081055206322205 1102 3 0.31752159986213613 1102 4 0.11845819761997944 1102 5 0.2676561619593443 1102 6 0.024755866121722647 1102 7 0.2753606622414466 1102 8 0.2423432045954712 1102 9 0.2275635744560347 1102 10-11 0.3510709061357957 1102 12-13 0.20444562796757282 1102 14-15 0.3193790299856687 1102 16-17 0.32216887630417546 1102 18-19 0.20575614119726993 1102 20-21 0.10610916073309795 1102 22-23 0.16520751531407285 1102 24-25 0.13181791224311468 1102 26-27 0.16952813543053225 1102 28-29 0.29914405243312814 1102 30-31 0.03417286635622929 1102 32-33 0.2340014295584112 1102 34-35 0.26593340855058045 1102 36-37 0.305043886067331 1102 38-39 0.19400274251217553 1102 40-41 0.033666240677604264 1102 42-43 0.28554983962118285 1102 44-45 0.4065507469555527 1102 46-47 0.4838873932939336 1102 48-49 0.42869239019325533 1102 50-51 0.35388394770099296 1102 52-53 0.4761267270730727 1102 54-55 0.4733067573884142 1102 56-57 0.5253263944497775 1102 58-59 0.5714816686537247 1102 60-61 0.5404610661381142 1102 62-63 0.4383836831761414 1102 64-65 0.5072545580522565 1102 66-67 0.49984605185933617 1102 68-69 0.49489476842525804 1102 70-71 0.39794568375155137 1102 72-73 0.44862693479212723 1102 74-75 0.587347831830364 1102 76-77 0.4144521430940884 1102 78-79 0.41876564353352563 1102 80-81 0.5448617959948869 1102 82-83 0.4465646618615544 1102 84-85 0.4312000968313079 1102 86-87 0.5062303183030288 1102 88-89 0.41471122138085903 1102 90-91 0.4468966076735157 1102 92-93 0.44507907215657383 1102 94-95 0.48708015249926717 1102 96-97 0.4396794408339453 1102 98-99 0.4433950275481813 1102 100-101 0.5071728049452311 1102 102-103 0.478793137108962 1102 104-105 0.3925773077722994 1102 106-107 0.39998304579412647 1102 108-109 0.3616385559878097 1102 110-111 0.4256742316785882 1102 112-113 0.41029477196564557 1102 114-115 0.42437674203865683 1102 116-117 0.4002551882473391 1102 118-119 0.35948977442199137 1102 120-121 0.2688153517449017 1102 122-123 0.19416544933973512 1102 124-125 0.11738968274416806 1103 1 0.13295805828807872 1103 2 0.6067373752395895 1103 3 0.41564681963523853 1103 4 0.23843349049655416 1103 5 0.4467157181564829 1103 6 0.29020244506421733 1103 7 0.3595707745802059 1103 8 0.3273861413193231 1103 9 0.33225885326713467 1103 10-11 0.429141252035528 1103 12-13 0.45330743464127465 1103 14-15 0.48578428233145843 1103 16-17 0.5759651674595858 1103 18-19 0.4351263871580713 1103 20-21 0.3991569278383764 1103 22-23 0.37148539179696627 1103 24-25 0.31520237174008514 1103 26-27 0.32065337371084013 1103 28-29 0.2454407600007329 1103 30-31 0.042705846201883446 1103 32-33 0.13478081437880007 1103 34-35 0.19323825003633033 1103 36-37 -0.039308176561064556 1103 38-39 -0.025468460271312665 1103 40-41 0.03405951885946479 1103 42-43 -0.09746262498377689 1103 44-45 -0.006026983320388979 1103 46-47 0.22171080676348254 1103 48-49 0.10893756443862657 1103 50-51 0.09769906994110755 1103 52-53 0.10872393618601706 1103 54-55 0.16226882686447652 1103 56-57 0.166110729948457 1103 58-59 0.16488986364567637 1103 60-61 0.21423565759148744 1103 62-63 0.10540430020647307 1103 64-65 0.10298772111927335 1103 66-67 0.172977517815319 1103 68-69 0.2992585368630607 1103 70-71 0.1788145509253134 1103 72-73 0.22371345599212944 1103 74-75 0.18102900814854195 1103 76-77 0.13612570369593868 1103 78-79 0.2083028245155205 1103 80-81 0.3415311924748181 1103 82-83 0.05291633583856736 1103 84-85 0.13640918201998886 1103 86-87 0.046721777226277794 1103 88-89 0.005649860730887468 1103 90-91 0.11076868584796529 1103 92-93 0.07177132503906591 1103 94-95 -0.02630749949118183 1103 96-97 -0.04578546024502472 1103 98-99 0.09051692527734545 1103 100-101 0.19809663852841197 1103 102-103 0.1736428123074525 1103 104-105 0.17880169842245763 1103 106-107 0.1976044068142464 1103 108-109 0.3100901966518492 1103 110-111 0.34202743250030565 1103 112-113 0.2650687064131141 1103 114-115 0.31727321271398523 1103 116-117 0.35009949635534454 1103 118-119 0.26586180632842016 1103 120-121 0.3141441323049321 1103 122-123 0.33921338300989845 1103 124-125 0.3271399742327006 1104 1 0.006115805201908131 1104 2 0.4295133604589694 1104 3 0.24581610991522496 1104 4 0.04932601153471694 1104 5 0.31611441686557384 1104 6 0.24418172504142888 1104 7 0.37788315627800273 1104 8 0.3100674315354155 1104 9 0.27105718246072286 1104 10-11 0.16347178471821167 1104 12-13 0.14967044901457172 1104 14-15 0.2265387408767765 1104 16-17 0.36595730061294773 1104 18-19 0.4302952980723518 1104 20-21 0.18453377774533664 1104 22-23 0.24156145794769657 1104 24-25 0.11615933835856396 1104 26-27 0.25665704224371666 1104 28-29 0.2834432198877934 1104 30-31 0.20123228998787113 1104 32-33 0.09312795795474216 1104 34-35 0.25492527599560333 1104 36-37 0.3072686802676543 1104 38-39 0.39557705163278456 1104 40-41 0.23257626552569377 1104 42-43 0.029429045476547344 1104 44-45 0.23362681552793418 1104 46-47 0.17517544721797407 1104 48-49 0.16178370569045342 1104 50-51 0.22725661639010752 1104 52-53 0.2962773468833362 1104 54-55 0.25361193452180686 1104 56-57 0.2588676403941861 1104 58-59 0.2697056117149046 1104 60-61 0.3418617667369972 1104 62-63 0.36265318007932024 1104 64-65 0.30694204282670867 1104 66-67 0.22116112149663536 1104 68-69 0.28019690790667795 1104 70-71 0.325779325821685 1104 72-73 0.28712676227735656 1104 74-75 0.21081620395715106 1104 76-77 0.30789966430047855 1104 78-79 0.3984337589265188 1104 80-81 0.20019866712387113 1104 82-83 0.1379501592857615 1104 84-85 0.16549205545560497 1104 86-87 0.15985894521473298 1104 88-89 0.11467274114150783 1104 90-91 0.007330967047135317 1104 92-93 0.09893341003295575 1104 94-95 0.025877580388595334 1104 96-97 0.20074201248723256 1104 98-99 0.18486101529217436 1104 100-101 0.05407594113445313 1104 102-103 0.00240528811456997 1104 104-105 -0.12796189285293025 1104 106-107 -0.08136149553036987 1104 108-109 -0.21047957735268596 1104 110-111 -0.1255785636075366 1104 112-113 -0.12076484655299424 1104 114-115 -0.056739105356868436 1104 116-117 -0.1267600278535106 1104 118-119 -0.07654701944821696 1104 120-121 -0.04873114023937575 1104 122-123 0.03658837202825893 1104 124-125 0.0566088023520841 1105 1 0.21568296714007928 1105 2 0.5694939314182186 1105 3 0.23146308006867855 1105 4 0.15861884019026462 1105 5 0.3095379749254903 1105 6 0.23875471867479092 1105 7 0.41133312351511364 1105 8 0.3507769395352298 1105 9 0.3027213484089941 1105 10-11 0.11785231749552594 1105 12-13 0.12871413054932646 1105 14-15 0.05718740634826247 1105 16-17 0.37093537085380035 1105 18-19 0.2961175869804009 1105 20-21 0.22486991804806422 1105 22-23 0.3255632238625594 1105 24-25 0.23250638040146043 1105 26-27 0.31496703388148006 1105 28-29 0.26099144199136504 1105 30-31 0.28460315895519983 1105 32-33 0.09401290402771423 1105 34-35 0.20024207177490183 1105 36-37 0.34721256076612406 1105 38-39 0.24162179013121943 1105 40-41 0.21324168531329235 1105 42-43 -0.00991774500302256 1105 44-45 0.18423290415578464 1105 46-47 0.005402023242297105 1105 48-49 0.0414290511226838 1105 50-51 -0.13005179527088728 1105 52-53 -0.13460190172784792 1105 54-55 -0.21831688001835658 1105 56-57 -0.17834543572807604 1105 58-59 -0.013429404209155393 1105 60-61 -0.179561882800499 1105 62-63 -0.1485354218152537 1105 64-65 -0.030898052389510156 1105 66-67 -0.0991212459031452 1105 68-69 -0.007973848901190905 1105 70-71 -0.040092181997160026 1105 72-73 -0.04850444788142738 1105 74-75 0.07616022425721525 1105 76-77 -0.06059088616581221 1105 78-79 -0.11393659398798661 1105 80-81 -0.070456620528347 1105 82-83 -0.09187481031286282 1105 84-85 -0.12215618544063744 1105 86-87 -0.05573181594826693 1105 88-89 -0.05861063748316653 1105 90-91 -0.09355089662941296 1105 92-93 0.008762376803730376 1105 94-95 0.04990287194849685 1105 96-97 0.05815333641627163 1105 98-99 0.02429003615403147 1105 100-101 -0.17813987434335132 1105 102-103 -0.14197565233452636 1105 104-105 -0.14213296187772784 1105 106-107 -0.13931701157821763 1105 108-109 -0.21007687717340673 1105 110-111 -0.09784843039829028 1105 112-113 -0.011678836754949629 1105 114-115 -0.0182509114323679 1105 116-117 0.010123231850322156 1105 118-119 0.03504915480064952 1105 120-121 -0.0392741491156805 1105 122-123 -0.03962686276583227 1105 124-125 0.20832485199258954 1106 1 0.23121904969423213 1106 2 0.5775696706686588 1106 3 0.35867365983949995 1106 4 0.1977580224017359 1106 5 0.33217000885270664 1106 6 0.276854996232899 1106 7 0.2680224289996005 1106 8 0.28900184688664154 1106 9 0.1985430522126208 1106 10-11 0.18955272918087473 1106 12-13 0.13617059734314552 1106 14-15 0.1324803735840021 1106 16-17 0.20473146255652352 1106 18-19 0.1374917709750676 1106 20-21 0.21199554410441834 1106 22-23 0.008977866991052963 1106 24-25 0.1270536815752692 1106 26-27 0.1279288231233906 1106 28-29 0.18955221016056356 1106 30-31 0.21569334983454524 1106 32-33 0.06288259127089901 1106 34-35 0.05768535843505873 1106 36-37 0.004106061409629547 1106 38-39 0.12366540862061726 1106 40-41 0.0024942554393589944 1106 42-43 0.07528745804856385 1106 44-45 0.0552197472811784 1106 46-47 0.04570948862506441 1106 48-49 -0.010889780187106624 1106 50-51 0.03400937217882927 1106 52-53 0.1246363059290374 1106 54-55 0.08770837932015496 1106 56-57 0.02196788318464371 1106 58-59 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-0.3149424028937844 2216 82-83 -0.4409465449234098 2216 84-85 -0.32929608606981375 2216 86-87 -0.2386359753634828 2216 88-89 -0.18347523714724545 2216 90-91 -0.06210142185006973 2216 92-93 -0.16015475611313335 2216 94-95 -0.3362361928662736 2216 96-97 -0.44401434745090995 2216 98-99 -0.4404878178005873 2216 100-101 -0.5653362523743759 2216 102-103 -0.4784667020886566 2216 104-105 -0.30766528616093325 2216 106-107 -0.2321996443776193 2216 108-109 -0.263640115402886 2216 110-111 -0.41372379017404626 2216 112-113 -0.18721040992387117 2216 114-115 -0.286013246087343 2216 116-117 -0.22520007490635052 2216 118-119 -0.36518321119337216 2216 120-121 -0.4514727245555257 2216 122-123 -0.41234207698522596 2216 124-125 -0.5497872622023507 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61.0 3 77.0 4 212.0 5 522.0 6 1353.0 7 2594.0 8 3897.0 9 6421.0 10 10581.0 11 13071.0 12 14462.0 13 18790.0 14 26090.0 15 19096.0 16 8605.0 17 7006.0 18 6938.0 19 6651.0 20 6575.0 21 6418.0 22 6942.0 23 7686.0 24 9279.0 25 10994.0 26 13456.0 27 16472.0 28 19575.0 29 23690.0 30 29181.0 31 36282.0 32 44424.0 33 55884.0 34 74843.0 35 107318.0 36 165816.0 37 242200.0 38 90302.0 39 292.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.73862600383886 17.32113232933729 12.648751382088847 19.291490284735 2 18.97638622219948 22.662566323146205 37.276951814680274 21.08409563997404 3 17.54509427272442 30.08280560654546 32.99469056734183 19.377409553388297 4 10.7235280125308 17.54581237012535 36.27442092176778 35.45623869557607 5 9.613496673525901 42.60096412509734 34.05704092456848 13.728498276808274 6 27.124399491587496 38.427870771307724 18.160487444078218 16.287242293026562 7 24.15462059357878 36.40445363608293 22.613674716531307 16.82725105380699 8 28.596497842119245 31.81455869363838 20.342155151985178 19.246788312257195 9 27.83325075220635 10.98750870692317 20.34412991806516 40.83511062280532 10-11 24.292495170799313 26.640492039897456 28.160388705774213 20.906624083529017 12-13 26.97552905778525 26.34046223888207 29.261365676411238 17.42264302692145 14-15 23.11943923824296 26.024320141895917 30.286224390874427 20.57001622898669 16-17 19.132521165901895 28.177353741643145 30.802850126025984 21.887274966428976 18-19 18.501718046489586 23.657966924463402 37.88795177262184 19.95236325642517 20-21 20.142658896859764 22.574089632837126 38.87897017744171 18.4042812928614 22-23 19.677421960835005 23.001402083916787 38.90616809208873 18.41500786315948 24-25 18.922702269903844 23.261801920190727 39.03924039725112 18.7762554126543 26-27 18.834421249919213 23.167461958824333 39.0000592429824 18.998057548274055 28-29 18.998237072463144 22.910383320048545 39.11118471602863 18.980194891459675 30-31 18.93576265466009 23.043769792541845 39.03811837106932 18.982349181728747 32-33 18.667508635113496 23.313460005601154 38.70855684094875 19.3104745183366 34-35 19.14334609002873 23.365801793537216 38.455220588202295 19.03563152823176 36-37 19.617640405868286 23.11692589959571 37.37832748084477 19.887106213691233 38-39 19.466077109229698 23.355109617469857 37.52746720093065 19.65134607236979 40-41 19.23997502817409 23.794326225219482 37.15954905298703 19.806149693619393 42-43 19.469469020193607 23.956971892683768 36.442429911240055 20.131129175882574 44-45 19.74068283740699 24.10066920992472 35.85158971582929 20.307058236838998 46-47 20.449658904526483 24.360922910358187 34.40880824410568 20.78060994100965 48-49 20.592513992108135 25.023890607277018 33.061548219078105 21.322047181536746 50-51 20.725876060022415 25.33555126551853 32.68071606413391 21.257856610325145 52-53 20.70679641282979 25.214413314541684 32.64410172625834 21.43468854637019 54-55 20.535116756623314 25.653242366776258 32.48789793193218 21.323742944668243 56-57 20.842214780433572 25.69001494192667 32.03712456108932 21.43064571655044 58-59 20.81108435573529 24.99150585389171 32.48578885594961 21.711620934423397 60-61 20.81686145670634 25.17872210552773 33.01350065528447 20.990915782481466 62-63 20.89475805474344 25.091312375845238 33.16831727605914 20.845612293352172 64-65 20.718043960354624 25.142624524641022 33.17950695068744 20.959824564316918 66-67 20.911980948183352 25.445943477396387 32.769456770984576 20.87261880343568 68-69 21.114953491242442 25.176800601416023 32.78282822615935 20.925417681182186 70-71 20.987228213547716 25.198695173686602 32.758133171995425 21.055943440770257 72-73 21.02922886828181 25.591226716611974 32.16863095181992 21.21091346328629 74-75 20.671716448624224 25.692175740311292 32.4171496304222 21.218958180642282 76-77 20.336161608908057 25.878968356376188 32.559338807366835 21.225531227348924 78-79 20.57707178861376 25.59378774217263 32.33411620875262 21.49502426046099 80-81 20.283559347517286 25.989753624716965 31.935389041360256 21.79129798640549 82-83 20.544299388899663 25.397646078832665 31.764875374307934 22.293179157959745 84-85 20.212153376972807 25.35603124628328 32.07564275471758 22.356172622026342 86-87 20.222978319283303 25.66485750921521 31.69476897225405 22.417395199247434 88-89 20.42809895322571 25.539084495505776 31.725344163570206 22.307472387698304 90-91 20.438986842961558 25.62352872534037 31.47335043321956 22.464133998478513 92-93 20.33851293794079 25.5990535425282 31.400369283577295 22.662064235953714 94-95 20.169266204925478 25.478706406569216 31.544759452947996 22.807267935557302 96-97 20.46980423028468 25.692963034939996 31.200582385617732 22.63665034915759 98-99 20.367498784833103 25.681694043640086 30.873856475212612 23.076950696314196 100-101 20.386456214554872 25.419596677336482 31.139154038522648 23.054793069585998 102-103 20.60140407506712 25.24260733203296 30.874391742509044 23.281596850390873 104-105 20.23880532707147 25.529433043802968 31.01686600771692 23.21489562140864 106-107 20.32038928199876 25.58388260501539 30.86012220266397 23.23560591032188 108-109 20.606350296330707 25.715760395137526 30.153158429772652 23.524730878759115 110-111 20.475610221333568 25.716523382186946 30.31289201264763 23.494974383831856 112-113 20.222726946279803 25.535137173226087 30.453309034760128 23.788826845733986 114-115 19.856714075491993 25.73934693078659 30.250801993558913 24.15313700016251 116-117 19.88436114696145 25.801470175635487 30.355154031760286 23.95901464564278 118-119 19.814911322710284 25.903257792339247 29.896350520267333 24.385480364683136 120-121 19.690873607916853 25.9522180868922 29.876353033901925 24.48055527128902 122-123 19.70501014165097 25.91370238260349 29.563664387881005 24.817623087864536 124-125 19.6824007558467 26.02778768966299 29.294086756300032 24.995724798190277 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 664.0 1 605.0 2 1748.5 3 3005.5 4 2142.0 5 1699.5 6 2864.5 7 7554.0 8 11825.5 9 12034.0 10 8938.5 11 5011.0 12 4020.0 13 3895.5 14 3921.0 15 4074.5 16 4211.5 17 4386.5 18 4628.0 19 4769.0 20 4928.5 21 5237.0 22 5731.5 23 6465.5 24 7184.0 25 7823.0 26 8998.0 27 10135.5 28 10954.0 29 12487.0 30 15096.0 31 18050.0 32 20744.5 33 22450.5 34 23671.0 35 25214.0 36 27621.5 37 30624.0 38 31887.0 39 31489.5 40 30415.0 41 29916.5 42 29626.5 43 30631.0 44 33750.5 45 35358.5 46 35132.5 47 34985.5 48 35059.0 49 34189.0 50 33369.5 51 31902.0 52 30344.0 53 30216.0 54 29878.0 55 28216.0 56 25990.0 57 23919.0 58 22392.0 59 20909.0 60 18460.5 61 16146.0 62 13790.5 63 10239.0 64 7227.0 65 5615.0 66 4236.5 67 3297.5 68 2483.0 69 1637.0 70 1152.0 71 956.0 72 955.5 73 1029.0 74 736.5 75 500.0 76 329.0 77 228.0 78 174.5 79 71.5 80 47.0 81 35.0 82 19.5 83 13.5 84 5.0 85 3.5 86 3.5 87 1.0 88 0.5 89 3.0 90 3.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06328227665395635 2 2.6928628363385684E-4 3 8.976209454461893E-5 4 8.976209454461893E-5 5 0.06417989759940254 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 4.4881047272309466E-5 36-37 0.0 38-39 0.0 40-41 4.4881047272309466E-5 42-43 0.003590483781784758 44-45 0.011713953338072772 46-47 0.013015503708969745 48-49 0.011175380770805059 50-51 0.009469900974457298 52-53 0.007719540130837229 54-55 0.00839275583992187 56-57 0.006866800232663349 58-59 0.004802272058137113 60-61 0.003231435403606282 62-63 0.002064528174526236 64-65 0.0026479817890662587 66-67 0.003725126923601686 68-69 0.0016605987490754506 70-71 0.0034109595926955197 72-73 0.0033660785454232103 74-75 0.0013015503708969747 76-77 7.62977803629261E-4 78-79 4.4881047272309466E-5 80-81 3.141673309061663E-4 82-83 0.0 84-85 3.141673309061663E-4 86-87 1.3464314181692842E-4 88-89 0.0013015503708969747 90-91 0.0012117882763523558 92-93 6.283346618123326E-4 94-95 0.0010771451345354273 96-97 0.0015708366545308316 98-99 0.001032264087263118 100-101 8.976209454461895E-4 102-103 0.0011669072290800464 104-105 9.425019927184989E-4 106-107 4.488104727230947E-4 108-109 0.0012117882763523558 110-111 0.0012117882763523558 112-113 0.012207644858068176 114-115 0.024146003432502495 116-117 0.021857070021614713 118-119 0.014137529890777484 120-121 0.007001443374480278 122-123 0.007674659083564919 124-125 0.0065975139490294914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1114056.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.167499950440366 #Duplication Level Percentage of deduplicated Percentage of total 1 49.657329221527796 12.497508307218016 2 13.910747386195531 7.00197468305329 3 9.566611674588316 7.2230309653825095 4 6.957925482038902 7.004543568975271 5 4.872241058715083 6.131106330187268 6 3.3223194080499576 5.016868412246662 7 2.4159154642060967 4.256178662797254 8 1.678488684334171 3.3794691103835968 9 1.2629442819981018 2.8606635139136554 >10 5.904117824603489 25.448667470634135 >50 0.29021264482016046 5.019824765239621 >100 0.15033164282481054 6.765518650806837 >500 0.006849643195097937 1.1281459476892055 >1k 0.0028840602926728156 1.8342125203864383 >5k 0.0 0.0 >10k+ 0.0010815226097523058 4.432287091086164 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17329 1.5554873363637016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15809 1.419048952655881 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15713 1.4104317915795974 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4339 0.3894777282291016 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3686 0.33086308049146546 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3516 0.31560352441888023 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2600 0.23338144581600923 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2147 0.19271921698729685 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1754 0.15744271383126163 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1152 0.10340593291540102 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 3.590483781784758E-4 0.0 0.0 9 0.0 0.0 3.590483781784758E-4 0.0 0.0 10-11 0.0 0.0 3.590483781784758E-4 4.488104727230947E-5 0.0 12-13 0.0 0.0 3.590483781784758E-4 8.976209454461895E-5 0.0 14-15 0.0 0.0 3.590483781784758E-4 4.936915199954043E-4 0.0 16-17 0.0 0.0 3.590483781784758E-4 5.385725672677137E-4 0.0 18-19 0.0 0.0 3.590483781784758E-4 7.62977803629261E-4 0.0 20-21 0.0 0.0 3.590483781784758E-4 8.976209454461895E-4 0.0 22-23 0.0 0.0 3.590483781784758E-4 0.0013913124654415937 0.0 24-25 0.0 0.0 3.590483781784758E-4 0.002199171316343164 0.0 26-27 0.0 0.0 3.590483781784758E-4 0.0026928628363385686 0.0 28-29 3.141673309061663E-4 0.0 3.590483781784758E-4 0.003814889018146305 0.0 30-31 6.283346618123326E-4 0.0 3.590483781784758E-4 0.007091205469024897 0.0 32-33 6.283346618123326E-4 0.0 3.590483781784758E-4 0.014676102458045199 0.0 34-35 6.283346618123326E-4 0.0 3.590483781784758E-4 0.025312910661582543 0.0 36-37 6.283346618123326E-4 0.0 3.590483781784758E-4 0.0416944929159755 0.0 38-39 6.283346618123326E-4 0.0 3.590483781784758E-4 0.07010419583934739 0.0 40-41 6.283346618123326E-4 0.0 3.590483781784758E-4 0.10354057605721795 0.0 42-43 6.283346618123326E-4 0.0 3.590483781784758E-4 0.13877219816598088 0.0 44-45 6.283346618123326E-4 0.0 3.590483781784758E-4 0.18131943098013026 0.0 46-47 9.873830399908083E-4 0.0 3.590483781784758E-4 0.24172932060865882 0.0 48-49 0.001346431418169284 0.0 3.590483781784758E-4 0.30312659327717817 0.0 50-51 0.001346431418169284 0.0 3.590483781784758E-4 0.3696403053347408 0.0 52-53 0.001346431418169284 0.0 3.590483781784758E-4 0.4412704567813467 0.0 54-55 0.001346431418169284 0.0 3.590483781784758E-4 0.5199918136969774 0.0 56-57 0.001346431418169284 0.0 3.590483781784758E-4 0.6293220448523233 0.0 58-59 0.001346431418169284 0.0 3.590483781784758E-4 0.7689469829164781 0.0 60-61 0.001346431418169284 0.0 3.590483781784758E-4 0.8936265322389538 0.0 62-63 0.001346431418169284 0.0 3.590483781784758E-4 1.0127857127469355 0.0 64-65 0.0014361935127139031 0.0 3.590483781784758E-4 1.1418187236548252 0.0 66-67 0.0017054797963477598 0.0 3.590483781784758E-4 1.3133989673768642 0.0 68-69 0.0017054797963477598 0.0 3.590483781784758E-4 1.547408747854686 0.0 70-71 0.0017054797963477598 0.0 3.590483781784758E-4 1.7731604156344027 0.0 72-73 0.0017054797963477598 0.0 3.590483781784758E-4 1.9784463258579459 0.0 74-75 0.0017054797963477598 0.0 3.590483781784758E-4 2.1941895200959376 0.0 76-77 0.0017054797963477598 0.0 3.590483781784758E-4 2.451447683060816 0.0 78-79 0.0017054797963477598 0.0 3.590483781784758E-4 2.799545085704848 0.0 80-81 0.0017054797963477598 0.0 3.590483781784758E-4 3.1495274923343173 0.0 82-83 0.0017054797963477598 0.0 3.590483781784758E-4 3.489636068563878 0.0 84-85 0.0017054797963477598 0.0 3.590483781784758E-4 3.8244486812153067 0.0 86-87 0.0017054797963477598 0.0 3.590483781784758E-4 4.181701817502891 0.0 88-89 0.0017054797963477598 0.0 3.590483781784758E-4 4.626472995971477 0.0 90-91 0.002064528174526236 0.0 3.590483781784758E-4 5.122722735661403 0.0 92-93 0.0028723870254278062 0.0 3.590483781784758E-4 5.608245905053247 0.0 94-95 0.0028723870254278062 0.0 3.590483781784758E-4 6.118319007303044 0.0 96-97 0.0028723870254278062 0.0 3.590483781784758E-4 6.621256023036544 0.0 98-99 0.0028723870254278062 0.0 3.590483781784758E-4 7.207088333082 0.0 100-101 0.0032763164508785914 0.0 3.590483781784758E-4 7.855798990355961 0.0 102-103 0.0036802458763293766 0.0 3.590483781784758E-4 8.50468917181901 0.0 104-105 0.0036802458763293766 0.0 3.590483781784758E-4 9.155823405645677 0.0 106-107 0.0036802458763293766 0.0 3.590483781784758E-4 9.801392389610577 0.0 108-109 0.0036802458763293766 0.0 3.590483781784758E-4 10.493413257502315 0.0 110-111 0.0036802458763293766 0.0 3.590483781784758E-4 11.255717845422492 0.0 112-113 0.0036802458763293766 0.0 3.590483781784758E-4 12.012277659291813 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7100 0.0 82.80687 1 GTATCAA 11690 0.0 50.263943 2 CCGTTGC 180 0.0 49.576992 9 GTACATG 10815 0.0 43.071278 1 ATGGGAG 2475 0.0 42.093662 5 TACATGG 11350 0.0 40.964634 2 TATAACG 175 0.0 40.822266 2 ACATGGG 11100 0.0 40.54644 3 CATGGGA 8370 0.0 37.76791 4 GTTATCA 645 0.0 36.017406 1 ATCAACG 17080 0.0 35.935585 4 TCAACGC 17160 0.0 35.76805 5 CATGGGG 2530 0.0 35.766544 4 CAACGCA 17425 0.0 35.26866 6 ATGGGAT 3135 0.0 35.130806 5 TATCAAC 17730 0.0 34.685303 3 TACCTGG 825 0.0 34.637074 2 AACGCAG 17795 0.0 34.53534 7 GTATAAC 305 0.0 33.20142 1 TATTCCG 270 0.0 33.073593 5 >>END_MODULE