SRR936231_2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR936231_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	44168
Sequences flagged as poor quality	0
Sequence length	125
%GC	42

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG	450	1.0188371671798586	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	298	0.6746966129324398	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	257	0.581869226589386	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	242	0.5479079876833907	No Hit
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT	229	0.5184749139648615	No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT	193	0.4369679405904728	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	172	0.38942220612207934	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	170	0.38489404093461327	No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT	160	0.3622532149972831	No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC	154	0.348668719434885	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT	147	0.3328201412787538	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	142	0.3214997283100888	No Hit
GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG	129	0.2920666545915595	No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	123	0.2784821590291614	No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT	118	0.2671617460604963	No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT	111	0.25131316790436514	No Hit
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT	97	0.21961601159210287	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	91	0.2060315160297048	No Hit
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA	81	0.18339069009237458	No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG	79	0.17886252490490853	No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT	78	0.17659844231117552	No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT	78	0.17659844231117552	No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	78	0.17659844231117552	No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG	72	0.1630139467487774	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	71	0.1607498641550444	No Hit
GAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC	70	0.15848578156131135	No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA	68	0.1539576163738453	No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA	67	0.1516935337801123	No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC	66	0.1494294511863793	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	65	0.14716536859264626	No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA	65	0.14716536859264626	No Hit
GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA	62	0.1403731208114472	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC	61	0.1381090382177142	No Hit
GTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCC	60	0.13584495562398116	No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT	59	0.13358087303024815	No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG	59	0.13358087303024815	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	59	0.13358087303024815	No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58	0.13131679043651512	No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT	57	0.12905270784278208	No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG	54	0.12226046006158305	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA	53	0.11999637746785002	No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT	52	0.117732294874117	No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA	50	0.11320412968665097	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT	50	0.11320412968665097	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	48	0.10867596449918493	No Hit
CCTTTTTCCTTTCCTTTTTCCTTTCCTTTTTCCTTTCCTTTTTCCTTTCC	48	0.10867596449918493	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA	47	0.10641188190545191	No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA	47	0.10641188190545191	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	45	0.10188371671798588	No Hit
GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	45	0.10188371671798588	No Hit
GAACAAAAAACACCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA	45	0.10188371671798588	No Hit
GTACATGGGGGCCGGGGGTCGGGCGCCGGCGGCTTTGGTGACTCTAGATA	45	0.10188371671798588	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCAACA	15	2.6850376E-4	118.99208	1
CCTTTAG	15	2.6850376E-4	118.99208	1
GTACATA	45	0.0	118.99207	1
TACATAA	45	3.947207E-10	92.549385	2
TACATGA	45	4.2093234E-8	79.32805	2
TACAAGA	30	4.2202955E-5	79.32805	2
GAACAAA	205	0.0	75.45839	1
TTTAGTG	25	0.0020442717	71.39524	5
ACTGACT	25	0.0020442717	71.39524	8
GCAACAA	25	0.0020442717	71.39524	2
CGCAGAA	25	0.0020442717	71.39524	2
TAGTGGA	25	0.0020442717	71.39524	7
GTACAAA	95	0.0	68.89015	1
GAGAACA	175	0.0	64.595695	1
GTCGGGA	175	0.0	64.595695	2
GACAATG	30	0.0042107673	59.49604	7
TCGGGAG	190	0.0	59.49604	3
AGAACAA	130	0.0	59.49604	2
GGGAACC	30	0.0042107673	59.49604	7
GTACTAG	30	0.0042107673	59.49604	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content