##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936231_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 44168 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.982159029161384 30.0 12.0 31.0 2.0 34.0 2 23.349144176779568 30.0 15.0 31.0 2.0 34.0 3 23.743751132041297 30.0 16.0 31.0 2.0 34.0 4 25.379460242709655 33.0 12.0 37.0 2.0 37.0 5 27.56357543923202 33.0 26.0 37.0 2.0 37.0 6 25.86795870313349 33.0 13.0 37.0 2.0 37.0 7 26.2715314254664 33.0 13.0 37.0 2.0 37.0 8 25.502422568375295 33.0 10.0 37.0 2.0 37.0 9 25.67920213729397 34.0 8.0 39.0 2.0 39.0 10-11 26.495981253396124 33.5 10.5 39.0 2.0 39.0 12-13 26.205363611664552 33.0 10.0 39.0 2.0 39.0 14-15 26.837382267705124 33.0 10.0 40.0 2.0 41.0 16-17 26.983143905089655 33.0 11.5 40.0 2.0 41.0 18-19 27.041794964680314 33.0 12.5 40.0 2.0 41.0 20-21 27.423643814526354 33.5 16.0 40.0 2.0 41.0 22-23 27.675941858358993 33.0 18.5 40.0 2.0 41.0 24-25 27.841061401919944 33.0 22.0 40.0 2.0 41.0 26-27 28.031165096902733 33.0 24.0 40.0 2.0 41.0 28-29 27.848940409346135 33.0 24.0 39.5 2.0 41.0 30-31 27.65230483608042 32.5 23.0 39.0 2.0 41.0 32-33 27.39670123166093 32.0 23.0 39.0 2.0 41.0 34-35 27.048383445028072 32.0 20.0 39.0 2.0 41.0 36-37 26.589850117732297 31.0 16.0 39.0 2.0 41.0 38-39 26.081903187828292 31.0 9.0 39.0 2.0 41.0 40-41 26.0520965404818 31.0 7.0 39.0 2.0 41.0 42-43 25.678771961601157 31.0 2.0 39.0 2.0 41.0 44-45 25.55519833363521 31.0 2.0 39.0 2.0 41.0 46-47 25.38542610034414 30.5 2.0 38.0 2.0 41.0 48-49 25.02772369136026 30.0 2.0 38.0 2.0 41.0 50-51 24.74840382177142 30.0 2.0 38.0 2.0 40.0 52-53 24.415515758014852 30.0 2.0 38.0 2.0 40.0 54-55 24.027078427821046 30.0 2.0 38.0 2.0 40.0 56-57 23.733189186741534 30.0 2.0 37.0 2.0 40.0 58-59 23.339906719797135 30.0 2.0 37.0 2.0 40.0 60-61 22.859264625973555 30.0 2.0 36.5 2.0 40.0 62-63 22.4225796957073 30.0 2.0 36.0 2.0 40.0 64-65 21.931783191450826 29.5 2.0 35.0 2.0 39.5 66-67 21.55860577793878 29.0 2.0 35.0 2.0 39.0 68-69 21.329084404999094 29.0 2.0 35.0 2.0 39.0 70-71 21.032297138199603 29.0 2.0 35.0 2.0 38.5 72-73 20.638527893497553 28.5 2.0 35.0 2.0 37.5 74-75 20.257663919579784 28.0 2.0 34.5 2.0 37.0 76-77 19.927017297591014 27.5 2.0 34.0 2.0 36.5 78-79 19.674979623256657 27.0 2.0 34.0 2.0 36.0 80-81 19.39863702227857 26.5 2.0 34.0 2.0 36.0 82-83 19.169625067922478 26.0 2.0 34.0 2.0 35.0 84-85 19.14612389059953 26.5 2.0 34.0 2.0 35.0 86-87 18.94327341061402 26.0 2.0 34.0 2.0 35.0 88-89 18.682802028618003 25.5 2.0 34.0 2.0 35.0 90-91 18.433786904546277 25.0 2.0 34.0 2.0 35.0 92-93 18.35887973193262 25.0 2.0 34.0 2.0 35.0 94-95 18.27071635573266 25.0 2.0 34.0 2.0 35.0 96-97 18.11378147074805 24.0 2.0 34.0 2.0 35.0 98-99 17.904704763629777 23.5 2.0 34.0 2.0 35.0 100-101 17.754596087665277 23.0 2.0 34.0 2.0 35.0 102-103 17.06894131497917 20.0 2.0 32.5 2.0 34.5 104-105 17.40707072994023 20.0 2.0 34.0 2.0 35.0 106-107 17.437692447020467 20.0 2.0 34.0 2.0 35.0 108-109 17.36219661293244 19.0 2.0 34.0 2.0 35.0 110-111 17.158451820322405 18.5 2.0 34.0 2.0 35.0 112-113 16.99073990219163 17.0 2.0 34.0 2.0 35.0 114-115 16.781323582684298 9.0 2.0 34.0 2.0 35.0 116-117 16.565205578699512 4.5 2.0 34.0 2.0 35.0 118-119 16.410557417134576 2.0 2.0 34.0 2.0 35.0 120-121 16.137067560224597 2.0 2.0 33.5 2.0 35.0 122-123 15.680096449918494 2.0 2.0 33.0 2.0 35.0 124-125 14.176756928092736 2.0 2.0 31.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.4756974352958565 1101 2 1.2460930823802379 1101 3 0.5173604429347982 1101 4 1.4281250256962288 1101 5 1.1005982854437306 1101 6 0.6368216387982599 1101 7 1.5900922341919674 1101 8 1.6340105690750875 1101 9 -0.5257177283657342 1101 10-11 1.705335022237552 1101 12-13 0.617646630923602 1101 14-15 0.9220040426986564 1101 16-17 0.6678926509662588 1101 18-19 1.6135670138162794 1101 20-21 0.7323613949383727 1101 22-23 1.6261251196825057 1101 24-25 1.0822286217793184 1101 26-27 1.6987945257408015 1101 28-29 2.129146426908953 1101 30-31 2.151262031087949 1101 32-33 2.278045164562343 1101 34-35 1.7531006947407803 1101 36-37 1.415423069144218 1101 38-39 1.9039006645036025 1101 40-41 1.6804549082507307 1101 42-43 1.5759487805737606 1101 44-45 1.1266315506856905 1101 46-47 1.4368260576605323 1101 48-49 1.3805741254096304 1101 50-51 0.9947995150605173 1101 52-53 1.1770430383164978 1101 54-55 0.7140640432345329 1101 56-57 1.2423081469280035 1101 58-59 1.2172550673416467 1101 60-61 1.5445222000683003 1101 62-63 1.266701922048835 1101 64-65 1.1367459275744274 1101 66-67 1.016579405497211 1101 68-69 1.2712895099875432 1101 70-71 0.31541633330604313 1101 72-73 0.5778419024203281 1101 74-75 -0.35384370928667863 1101 76-77 0.3216772182585643 1101 78-79 1.070291898956313 1101 80-81 0.4933753352224386 1101 82-83 0.527656075602696 1101 84-85 0.418241154739043 1101 86-87 0.23362912551938209 1101 88-89 -1.0726139113772177 1101 90-91 -0.16884416480390385 1101 92-93 0.22686335684166892 1101 94-95 0.04624006148095816 1101 96-97 -0.25747021011080307 1101 98-99 -0.016329264667241716 1101 100-101 -0.19895884737335834 1101 102-103 -0.3922091505724623 1101 104-105 -0.022679748833908775 1101 106-107 0.2824570473932546 1101 108-109 0.4419831524340516 1101 110-111 0.42791449357775946 1101 112-113 0.16224687066280907 1101 114-115 0.25238818754120373 1101 116-117 0.2753563714455822 1101 118-119 0.18286470617035278 1101 120-121 0.06985408344745281 1101 122-123 0.7606486022801082 1101 124-125 0.02104630740730684 1102 1 0.0711301276035492 1102 2 0.7158046208417765 1102 3 0.25630275062710695 1102 4 0.8036057949269981 1102 5 -0.1359401760947314 1102 6 -1.0751975919709693 1102 7 0.10403454188427474 1102 8 -0.4116625078479892 1102 9 -0.19927542067342685 1102 10-11 -0.5243524777624486 1102 12-13 0.01692547707745007 1102 14-15 0.044600196544809734 1102 16-17 -0.3239342721106624 1102 18-19 -0.5462887554144871 1102 20-21 -0.5039366819847046 1102 22-23 -0.360653726471341 1102 24-25 -0.2742617628360655 1102 26-27 -0.07776797425919568 1102 28-29 -0.3037862653987382 1102 30-31 -0.8576321996812801 1102 32-33 -1.4527240662068905 1102 34-35 -1.4184137283361409 1102 36-37 -1.6961153923942405 1102 38-39 -1.6398493354963968 1102 40-41 -1.3842085532877313 1102 42-43 -1.265878142503162 1102 44-45 -1.727935757006616 1102 46-47 -2.173390288493316 1102 48-49 -1.9243537592057542 1102 50-51 -1.9543591387856374 1102 52-53 -1.221755038606581 1102 54-55 -1.3638205721500825 1102 56-57 -1.0699514684566118 1102 58-59 -0.41159108650450804 1102 60-61 -0.797545107624007 1102 62-63 -1.1712788471819344 1102 64-65 -1.062653110887112 1102 66-67 -0.9585407868104809 1102 68-69 -0.4952970284739955 1102 70-71 -0.41607405130934083 1102 72-73 -0.8828552129642873 1102 74-75 -1.1421850554405246 1102 76-77 -1.0733948971260503 1102 78-79 -0.3941311779667629 1102 80-81 -0.25037466477756 1102 82-83 -0.18171892439730541 1102 84-85 0.1430007701236562 1102 86-87 -1.1194958744806165 1102 88-89 -1.2943687190695243 1102 90-91 -0.5467287801885199 1102 92-93 -0.5713337585429485 1102 94-95 -0.5013560923651958 1102 96-97 -0.9901625178031104 1102 98-99 -1.180752341590317 1102 100-101 -0.919391539681051 1102 102-103 -0.24569472749553967 1102 104-105 -0.20885763344929487 1102 106-107 -0.779562183375976 1102 108-109 -0.7140264629505637 1102 110-111 -0.6481672371914726 1102 112-113 -0.6149165908756551 1102 114-115 -1.3684050816895663 1102 116-117 -0.3659897824005718 1102 118-119 0.03803297540111927 1102 120-121 0.5307915834474528 1102 122-123 0.0642543715108772 1102 124-125 0.7047001535611521 1103 1 0.6232668797402994 1103 2 0.9162319712691271 1103 3 1.034721554045909 1103 4 1.8204861368073395 1103 5 0.2533760632215092 1103 6 2.4527938610204814 1103 7 1.6074533453030782 1103 8 1.5263716801861982 1103 9 2.5124767160787087 1103 10-11 2.699258633348663 1103 12-13 1.7721605198124912 1103 14-15 2.1476984871430993 1103 16-17 2.365809317632923 1103 18-19 1.000719791594058 1103 20-21 1.3729863949383727 1103 22-23 1.3743890085713915 1103 24-25 2.4728536217793184 1103 26-27 1.0416764701852443 1103 28-29 2.217688093575621 1103 30-31 2.4203592533101705 1103 32-33 2.7294340534512322 1103 34-35 2.4032743058518946 1103 36-37 2.3737564024775537 1103 38-39 3.108761775614717 1103 40-41 2.8106632415840664 1103 42-43 1.954421002795982 1103 44-45 2.6630898840190262 1103 46-47 2.606096890993868 1103 48-49 2.608872736520741 1103 50-51 2.6536536817271816 1103 52-53 2.269056927205387 1103 54-55 2.20711959879009 1103 56-57 1.917655369150225 1103 58-59 2.7866995117860895 1103 60-61 2.817091644512743 1103 62-63 3.652118588715499 1103 64-65 2.8771973164633167 1103 66-67 2.5573780166083218 1103 68-69 2.575108954431986 1103 70-71 2.0115968888616003 1103 72-73 2.0908627357536638 1103 74-75 2.9178576796022107 1103 76-77 3.0100452738141215 1103 78-79 2.0494585656229773 1103 80-81 2.302403113000217 1103 82-83 1.855781075602696 1103 84-85 1.3887272658501573 1103 86-87 2.1277263477416035 1103 88-89 1.9786013664005608 1103 90-91 1.9700447240849854 1103 92-93 2.3648841901750046 1103 94-95 1.9386011725920689 1103 96-97 2.24947423433364 1103 98-99 1.879504068666094 1103 100-101 1.5909717081821988 1103 102-103 2.21456168276087 1103 104-105 2.049368862277202 1103 106-107 1.8414848251710332 1103 108-109 2.32913593021183 1103 110-111 2.0242686602444238 1103 112-113 2.209121870662809 1103 114-115 2.4216590208745394 1103 116-117 1.9906341492233608 1103 118-119 1.6715799839481313 1103 120-121 1.8085693612252314 1103 122-123 1.5505791578356654 1103 124-125 1.8335463074073068 1104 1 1.8078546933603725 1104 2 1.4870205017350777 1104 3 1.1453846364831861 1104 4 2.253729864405905 1104 5 1.5804369951211505 1104 6 0.9684748646047119 1104 7 1.8673099761274514 1104 8 1.5684863755266996 1104 9 1.838193561956846 1104 10-11 1.396615264173036 1104 12-13 1.31270711479457 1104 14-15 1.2455927523760728 1104 16-17 2.586239425159807 1104 18-19 2.5293936267195036 1104 20-21 1.8487928465512766 1104 22-23 0.550016248714762 1104 24-25 1.2006762024244786 1104 26-27 0.32707073541821785 1104 28-29 0.5207794914250812 1104 30-31 -0.5478307108475349 1104 32-33 -0.06872902898604494 1104 34-35 -0.2582400310656716 1104 36-37 -0.14909305988803823 1104 38-39 0.5278925999874744 1104 40-41 0.9128137792184745 1104 42-43 1.4055858773479528 1104 44-45 1.5636275184276265 1104 46-47 1.3776022673379487 1104 48-49 1.1328422705709187 1104 50-51 0.8478741118347095 1104 52-53 0.5858131996068181 1104 54-55 1.2483382367829208 1104 56-57 1.8970460501538113 1104 58-59 2.261609906051323 1104 60-61 2.4311149420037843 1104 62-63 2.338274502693995 1104 64-65 2.3290342340260395 1104 66-67 2.327315292593987 1104 68-69 2.5721967680520628 1104 70-71 3.268541333306043 1104 72-73 2.342206821775168 1104 74-75 2.573323629423001 1104 76-77 2.9403768150327565 1104 78-79 3.431178995730505 1104 80-81 3.964141464254695 1104 82-83 3.458603656247856 1104 84-85 3.785680670868075 1104 86-87 3.135342835196802 1104 88-89 3.7713881047518143 1104 90-91 2.986397770679968 1104 92-93 3.0053411794223166 1104 94-95 4.258941674384182 1104 96-97 3.585271725373069 1104 98-99 3.8526223482359825 1104 100-101 3.4809806687556737 1104 102-103 3.171550930072698 1104 104-105 4.149951299553187 1104 106-107 3.7108844667480945 1104 108-109 3.6481323459824395 1104 110-111 3.869698767771311 1104 112-113 4.243396064211197 1104 114-115 3.4250192359283 1104 116-117 3.24914669402623 1104 118-119 2.897834464234869 1104 120-121 1.9501464221571325 1104 122-123 2.2853461829252684 1104 124-125 2.523062436439563 1105 1 1.2242769807504033 1105 2 1.2318885369256911 1105 3 0.6849740792984349 1105 4 0.788920480241682 1105 5 1.4377194975649417 1105 6 0.9161776994043223 1105 7 1.7539179917677252 1105 8 1.1046545084690251 1105 9 2.1627292413312347 1105 10-11 2.449889946479974 1105 12-13 2.2147110248629964 1105 14-15 2.1710570730016876 1105 16-17 2.312779014602622 1105 18-19 2.609305650179916 1105 20-21 2.5093500313020094 1105 22-23 2.6673183015006856 1105 24-25 3.213383924809623 1105 26-27 3.358595662104438 1105 28-29 3.0339759723635 1105 30-31 3.709500667451586 1105 32-33 3.444711831229011 1105 34-35 3.5137541038316904 1105 36-37 3.172998826719976 1105 38-39 3.666211270564208 1105 40-41 3.295511726432551 1105 42-43 3.2814412048161827 1105 44-45 3.102483823412964 1105 46-47 2.96215749705447 1105 48-49 3.0981404132884194 1105 50-51 3.4358506514241505 1105 52-53 2.5581983413468024 1105 54-55 2.6288367705072595 1105 56-57 2.8166452681401246 1105 58-59 1.6452853703719512 1105 60-61 1.3859047758258782 1105 62-63 1.366133740230655 1105 64-65 1.3424750942410917 1105 66-67 0.9292214509517578 1105 68-69 0.6249826918057231 1105 70-71 1.0791473939121055 1105 72-73 1.2840445539354768 1105 74-75 1.1539687907133214 1105 76-77 0.8919897182585643 1105 78-79 0.45097371713813317 1105 80-81 0.17235260794971197 1105 82-83 0.3273719846936025 1105 84-85 0.37610100322389073 1105 86-87 1.4850495800648353 1105 88-89 1.11180843710763 1105 90-91 1.1550194715597328 1105 92-93 0.9198084325992468 1105 94-95 1.2555203645112627 1105 96-97 1.8372267595861658 1105 98-99 1.182534371696395 1105 100-101 1.2229161526266417 1105 102-103 0.7069859251851156 1105 104-105 -0.06363618822784645 1105 106-107 0.4178737140599189 1105 108-109 1.3392369403128406 1105 110-111 0.9826019935777595 1105 112-113 0.9420764161173558 1105 114-115 0.38567417238968815 1105 116-117 0.29997758356679327 1105 118-119 0.3445597819279307 1105 120-121 0.34139764405351514 1105 122-123 0.6844175416740459 1105 124-125 -0.21569611683511702 1106 1 -1.0820471299215342 1106 2 -0.3965699610980238 1106 3 -0.7702301367753464 1106 4 -2.5134510612602945 1106 5 -1.3178799754258357 1106 6 -1.6879066220713064 1106 7 -1.380469360010931 1106 8 -1.4651742135336079 1106 9 -1.5968228008295036 1106 10-11 -1.833048129936362 1106 12-13 -3.0524167748734996 1106 14-15 -2.848376392083953 1106 16-17 -1.9095530012076551 1106 18-19 -1.8567681311112594 1106 20-21 -2.329912155786264 1106 22-23 -2.572470894810248 1106 24-25 -5.785298552133725 1106 26-27 -3.6763186988968783 1106 28-29 -3.315826399178004 1106 30-31 -3.3761383312308944 1106 32-33 -6.301664980365196 1106 34-35 -2.99678608062154 1106 36-37 -2.327330554044188 1106 38-39 -2.092023248539874 1106 40-41 -2.145858497546371 1106 42-43 -2.4688881759479777 1106 44-45 -1.9999535942418483 1106 46-47 -1.9881060075568584 1106 48-49 -1.7295934470541354 1106 50-51 -1.758483999432233 1106 52-53 -2.5926580486400255 1106 54-55 -2.210754797345178 1106 56-57 -1.9386248240864887 1106 58-59 -2.1504985558467595 1106 60-61 -2.066664394134598 1106 62-63 -2.7165408315743527 1106 64-65 -3.099553891266151 1106 66-67 -3.467682369865109 1106 68-69 -2.694516649432746 1106 70-71 -3.2169659130707693 1106 72-73 -2.6863111772898165 1106 74-75 -3.088784832475085 1106 76-77 -3.184097238263174 1106 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2215 124-125 -0.5771679783069779 2216 1 2.7361994844761846 2216 2 2.0724250495933525 2216 3 1.1387743773610275 2216 4 1.752407812581474 2216 5 1.4960900887224184 2216 6 1.8801618027326867 2216 7 2.979948791569015 2216 8 2.3373917166160716 2216 9 2.2668027634375463 2216 10-11 1.0953196533850935 2216 12-13 3.013394581743274 2216 14-15 2.15304912466587 2216 16-17 2.8302901919498638 2216 18-19 3.231138735127754 2216 20-21 2.594297870348207 2216 22-23 2.0264837262398814 2216 24-25 2.361173293910465 2216 26-27 3.1402648126260466 2216 28-29 2.5213083121548543 2216 30-31 2.925596047481392 2216 32-33 2.220668115382015 2216 34-35 1.6948271291670096 2216 36-37 2.0178820855376607 2216 38-39 2.412609680897045 2216 40-41 2.0592971213654856 2216 42-43 2.570313534672124 2216 44-45 2.5961499933086394 2216 46-47 1.7235832297916787 2216 48-49 1.6996058877047133 2216 50-51 1.4470279986670747 2216 52-53 1.8207418088083003 2216 54-55 1.4548427317591255 2216 56-57 1.63395773709194 2216 58-59 1.547173100128532 2216 60-61 1.416704577117482 2216 62-63 1.1258207745078543 2216 64-65 1.52826744396787 2216 66-67 0.7075373973004915 2216 68-69 0.8611972968727919 2216 70-71 1.2603446119945652 2216 72-73 0.8784310417645891 2216 74-75 1.1826573153034872 2216 76-77 0.994448734652007 2216 78-79 0.7352509153497557 2216 80-81 0.42831385981260084 2216 82-83 0.8117236985535143 2216 84-85 0.519419433427565 2216 86-87 -0.09654505480848741 2216 88-89 -0.07684034580344701 2216 90-91 1.1784919007698669 2216 92-93 0.8194606929072421 2216 94-95 0.6983917008252192 2216 96-97 1.0191691341514932 2216 98-99 0.8781379484475131 2216 100-101 1.0548833657413965 2216 102-103 1.352539824837372 2216 104-105 1.654446275756257 2216 106-107 1.8905513096883375 2216 108-109 1.6620138901389687 2216 110-111 1.9826019935777595 2216 112-113 0.9662940018103505 2216 114-115 0.3598420809838281 2216 116-117 0.35834817472427005 2216 118-119 0.5720808946949454 2216 120-121 0.8914473211523699 2216 122-123 0.962236717034207 2216 124-125 0.12248073363681478 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 5950.0 3 762.0 4 657.0 5 690.0 6 1109.0 7 1044.0 8 936.0 9 922.0 10 944.0 11 1013.0 12 902.0 13 726.0 14 634.0 15 580.0 16 584.0 17 522.0 18 436.0 19 380.0 20 339.0 21 352.0 22 345.0 23 490.0 24 768.0 25 968.0 26 975.0 27 735.0 28 780.0 29 957.0 30 1009.0 31 1240.0 32 1499.0 33 1824.0 34 2364.0 35 2963.0 36 3338.0 37 3390.0 38 1038.0 39 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 57.39126497158512 17.78024316797609 8.757669753435824 16.070822107002964 2 17.562488679587034 30.06928092736823 32.519018293787354 19.849212099257382 3 17.920213729396846 44.67714182213367 23.458159753667815 13.944484694801668 4 9.013516879117892 23.368125523580954 26.72810016528177 40.89025743201938 5 9.957660696900401 57.497679262798016 20.62399529060158 11.920664749700002 6 20.653429030724297 40.59818416464782 20.940974030384677 17.807412774243215 7 25.08037857175203 49.531313680206495 12.597926006430285 12.790381741611196 8 23.75130190644387 45.10709595616538 13.727754381198206 17.413847756192546 9 26.651723044876146 31.218584431463114 12.344337273015443 29.78535525064529 10-11 20.57238599827922 42.38328125707558 18.466693836888105 18.5776389077571 12-13 25.77548340352307 39.40474573201105 17.893855001584928 16.92591586288095 14-15 23.37005128437355 38.08515696641043 18.86200767567445 19.682784073541566 16-17 21.300321514287006 38.148122990535704 20.49879998188652 20.052755513290766 18-19 21.549381877462302 35.981297830910655 23.775075850201514 18.694244441425532 20-21 27.008332201240776 30.857446904858943 20.797672417696873 21.336548476203415 22-23 25.849584697843603 32.47515552584165 19.582091581774296 22.093168194540446 24-25 24.526785309966943 31.996332020105967 21.832405017434226 21.64447765249287 26-27 25.49669998754712 31.664270431209175 22.07102668311956 20.768002898124145 28-29 22.94725640474115 30.61483250880192 25.735568813467218 20.70234227298971 30-31 23.737448065841757 33.20842720161208 23.424994056581347 19.629130675964817 32-33 21.36824706788027 33.96051261151112 21.577684191459493 23.09355612914912 34-35 22.557820972909333 31.851063588919203 24.53443220540455 21.056683232766915 36-37 23.27416396857382 31.954853170919467 22.524735662372358 22.246247198134352 38-39 25.288397314706845 31.641628836335233 22.980086717308367 20.089887131649554 40-41 23.009520790644494 33.13257785878437 24.400846795648285 19.45705455492285 42-43 20.605216682515962 35.87488113028121 23.713942851967577 19.805959335235247 44-45 20.764841733460127 35.54997056559344 22.63619073495449 21.04899696599194 46-47 21.009373726395868 35.425440384005796 21.355794049721506 22.209391839876826 48-49 21.077954128648084 35.33973328503181 21.24550003396201 22.336812552358097 50-51 21.930648794901114 35.09333997486783 22.977822557820975 19.998188672410084 52-53 21.306872856123984 36.05334359752301 21.93970543285069 20.700078113502315 54-55 19.870488611148847 36.259792600643024 23.60979033645791 20.259928451750213 56-57 21.389756826518138 36.926595118416884 20.7580491781008 20.92559887696418 58-59 19.901055110265816 35.020151247566 21.690893447448264 23.387900194719922 60-61 20.32083139936377 35.774851980573516 21.133664655338322 22.770651964724394 62-63 22.472037313770773 34.500520762577544 20.771634288819456 22.255807634832223 64-65 20.33577865326269 34.76882669927093 22.414300593216502 22.481094054249876 66-67 19.343408614931793 34.93518990207732 21.72864662930888 23.992754853682005 68-69 20.017434028052936 34.837489952792275 21.790270906682665 23.35480511247212 70-71 18.97500339627768 35.137888873794324 21.92297242222524 23.964135307702758 72-73 19.938869077941927 33.54502745231222 21.089036055923476 25.42706741382238 74-75 19.762716085721078 35.14088732410311 21.894422243102802 23.201974347073005 76-77 20.850880768011592 34.54806865009283 22.97129013268125 21.629760449214327 78-79 20.755315054226006 32.65447053229787 24.757171643987594 21.833042769488532 80-81 19.705889012158398 34.93332125795277 23.714538003486766 21.646251726402067 82-83 19.040233658613896 34.58238051033577 21.512667828922048 24.864718002128285 84-85 20.519845133244278 31.98089071025879 23.276428102429417 24.222836054067518 86-87 22.187379717888923 32.56843344578532 22.453415445921163 22.7907713904046 88-89 23.119332525783115 29.637847689991283 24.497073573862544 22.74574621036306 90-91 21.21606629383929 32.05447506056558 23.72359453890914 23.005864106685987 92-93 19.605357001347176 33.69522149140185 22.838576749346224 23.860844757904747 94-95 19.364004799963773 33.8363484049177 23.77000928294881 23.02963751216972 96-97 19.80664296873231 34.49634342382322 21.342857789752532 24.354155817691943 98-99 19.159100686032556 35.21747005682976 21.011162179908077 24.612267077229603 100-101 19.43532501641497 36.71745873616048 22.033418615708563 21.813797631715985 102-103 19.098401485305438 38.29982339356066 21.862971516551195 20.738803604582714 104-105 17.14175742069871 39.93026467724772 22.8620916068558 20.06588629519777 106-107 16.330065433468427 41.89666493083071 21.337197455113547 20.436072180587317 108-109 16.377983063895304 42.322148258841644 19.082552189467012 22.217316487796044 110-111 16.52741928180048 41.83421636553005 20.888239822487886 20.750124530181587 112-113 17.74628116014219 41.99741888740463 20.00701881495234 20.249281137500848 114-115 17.295914324204997 42.8378975015 19.132147668481768 20.73404050581323 116-117 17.165530826182444 41.92043833631444 20.661353499218873 20.25267733828424 118-119 16.510437893402163 43.22669021419191 20.50898881492551 19.753883077480413 120-121 16.880249960377657 41.35553693934386 19.8813593859669 21.88285371431159 122-123 17.767991577326704 40.57034177487463 18.939694112053253 22.72197253574542 124-125 17.651187538207257 40.77025833767292 19.500984898227184 22.077569225892635 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 76.0 1 103.0 2 129.5 3 134.5 4 128.5 5 121.0 6 114.5 7 98.0 8 103.5 9 114.5 10 103.5 11 83.5 12 68.0 13 73.5 14 90.0 15 85.0 16 79.5 17 83.5 18 96.5 19 110.5 20 117.5 21 153.5 22 178.5 23 213.0 24 275.5 25 350.0 26 431.5 27 497.5 28 588.0 29 658.5 30 690.5 31 733.5 32 901.5 33 1135.5 34 1294.0 35 1352.0 36 1404.5 37 1570.0 38 1498.5 39 1289.5 40 1143.5 41 1011.0 42 941.5 43 916.0 44 1001.0 45 1040.5 46 1017.5 47 1122.5 48 1486.0 49 1484.5 50 1296.5 51 1168.5 52 858.0 53 733.0 54 692.5 55 715.5 56 819.5 57 903.0 58 1034.0 59 1143.5 60 1327.5 61 1460.5 62 1161.5 63 615.5 64 299.5 65 229.5 66 160.0 67 111.0 68 105.5 69 102.0 70 99.5 71 124.0 72 125.5 73 115.5 74 94.0 75 63.5 76 40.5 77 27.0 78 22.0 79 18.5 80 14.0 81 9.5 82 5.5 83 3.0 84 2.0 85 2.0 86 1.5 87 2.0 88 2.0 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0022640825937330195 2 0.0 3 0.0 4 0.0022640825937330195 5 0.0022640825937330195 6 0.0022640825937330195 7 0.004528165187466039 8 0.004528165187466039 9 0.004528165187466039 10-11 0.004528165187466039 12-13 0.004528165187466039 14-15 0.005660206484332548 16-17 0.004528165187466039 18-19 0.004528165187466039 20-21 0.004528165187466039 22-23 0.09848759282738634 24-25 0.004528165187466039 26-27 0.003396123890599529 28-29 0.003396123890599529 30-31 0.003396123890599529 32-33 0.004528165187466039 34-35 0.003396123890599529 36-37 0.0022640825937330195 38-39 0.003396123890599529 40-41 0.003396123890599529 42-43 0.004528165187466039 44-45 0.004528165187466039 46-47 0.004528165187466039 48-49 0.0022640825937330195 50-51 0.003396123890599529 52-53 0.003396123890599529 54-55 0.004528165187466039 56-57 0.004528165187466039 58-59 0.004528165187466039 60-61 0.003396123890599529 62-63 0.004528165187466039 64-65 0.004528165187466039 66-67 0.0011320412968665098 68-69 0.003396123890599529 70-71 0.004528165187466039 72-73 0.0011320412968665098 74-75 0.003396123890599529 76-77 0.004528165187466039 78-79 0.0022640825937330195 80-81 0.0022640825937330195 82-83 0.0022640825937330195 84-85 0.0022640825937330195 86-87 0.0022640825937330195 88-89 0.003396123890599529 90-91 0.0022640825937330195 92-93 0.003396123890599529 94-95 0.0022640825937330195 96-97 0.0022640825937330195 98-99 0.0022640825937330195 100-101 0.0022640825937330195 102-103 0.004528165187466039 104-105 0.0022640825937330195 106-107 0.0022640825937330195 108-109 0.004528165187466039 110-111 0.004528165187466039 112-113 0.0022640825937330195 114-115 0.003396123890599529 116-117 0.0022640825937330195 118-119 0.004528165187466039 120-121 0.0022640825937330195 122-123 0.003396123890599529 124-125 0.0022640825937330195 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 44168.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.9930266256113 #Duplication Level Percentage of deduplicated Percentage of total 1 82.50778375318426 46.19860532512226 2 7.403663418381788 8.291070458250317 3 3.4814605151429383 5.8481253396123885 4 1.9691884679147627 4.410432892591921 5 1.26966155836804 3.5546096721608405 6 0.8208321539767903 2.7576525991668177 7 0.5580041243783107 2.187103785546097 8 0.32752415996118234 1.4671255207389966 9 0.2547410133031418 1.283734830646622 >10 1.237313493186689 13.00941858358993 >50 0.10513121183939186 3.984785364970114 >100 0.06469613036270268 7.007335627603695 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 450 1.0188371671798586 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 298 0.6746966129324398 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 257 0.581869226589386 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 242 0.5479079876833907 No Hit GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT 229 0.5184749139648615 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 193 0.4369679405904728 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 172 0.38942220612207934 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 170 0.38489404093461327 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 160 0.3622532149972831 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 154 0.348668719434885 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 147 0.3328201412787538 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 142 0.3214997283100888 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 129 0.2920666545915595 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 123 0.2784821590291614 No Hit GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT 118 0.2671617460604963 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 111 0.25131316790436514 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 97 0.21961601159210287 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 91 0.2060315160297048 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 81 0.18339069009237458 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 79 0.17886252490490853 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 78 0.17659844231117552 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 78 0.17659844231117552 No Hit CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT 78 0.17659844231117552 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 72 0.1630139467487774 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 71 0.1607498641550444 No Hit GAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC 70 0.15848578156131135 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 68 0.1539576163738453 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 67 0.1516935337801123 No Hit CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC 66 0.1494294511863793 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 65 0.14716536859264626 No Hit GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA 65 0.14716536859264626 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 62 0.1403731208114472 No Hit CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC 61 0.1381090382177142 No Hit GTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCC 60 0.13584495562398116 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 59 0.13358087303024815 No Hit GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 59 0.13358087303024815 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 59 0.13358087303024815 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 58 0.13131679043651512 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 57 0.12905270784278208 No Hit GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG 54 0.12226046006158305 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 53 0.11999637746785002 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 52 0.117732294874117 No Hit GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA 50 0.11320412968665097 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 50 0.11320412968665097 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 48 0.10867596449918493 No Hit CCTTTTTCCTTTCCTTTTTCCTTTCCTTTTTCCTTTCCTTTTTCCTTTCC 48 0.10867596449918493 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 47 0.10641188190545191 No Hit CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA 47 0.10641188190545191 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 45 0.10188371671798588 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 45 0.10188371671798588 No Hit GAACAAAAAACACCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 45 0.10188371671798588 No Hit GTACATGGGGGCCGGGGGTCGGGCGCCGGCGGCTTTGGTGACTCTAGATA 45 0.10188371671798588 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0022640825937330195 0.0 18-19 0.0 0.0 0.0 0.0022640825937330195 0.0 20-21 0.0 0.0 0.0 0.0022640825937330195 0.0 22-23 0.0 0.0 0.0 0.003396123890599529 0.0 24-25 0.0 0.0 0.0 0.009056330374932078 0.0 26-27 0.0 0.0 0.0 0.009056330374932078 0.0 28-29 0.0 0.0 0.0 0.014716536859264627 0.0 30-31 0.0 0.0 0.0 0.04075348668719435 0.0 32-33 0.0 0.0 0.0 0.08490309726498824 0.0 34-35 0.0 0.0 0.0 0.1392410795145807 0.0 36-37 0.0 0.0 0.0 0.21508784640463685 0.0 38-39 0.0 0.0 0.0 0.32036768701322227 0.0 40-41 0.0 0.0 0.0 0.4448922296685383 0.0 42-43 0.0 0.0 0.0 0.5773410614019199 0.0 44-45 0.0 0.0 0.0 0.7290345951820323 0.0 46-47 0.0 0.0 0.0 0.8943126245245426 0.0 48-49 0.0 0.0 0.0 1.0765712733200508 0.0 50-51 0.0 0.0 0.0 1.239585220068828 0.0 52-53 0.0 0.0 0.0 1.4252399927549355 0.0 54-55 0.0 0.0 0.0 1.645988045643905 0.0 56-57 0.0 0.0 0.0 1.8939050896576708 0.0 58-59 0.0 0.0 0.0 2.1893678681398296 0.0 60-61 0.0 0.0 0.0 2.518791885527984 0.0 62-63 0.0 0.0 0.0 2.7904817967759463 0.0 64-65 0.0 0.0 0.0 3.066699873211375 0.0 66-67 0.0 0.0 0.0 3.3995200144901285 0.0 68-69 0.0 0.0 0.0 3.7742256837529435 0.0 70-71 0.0 0.0 0.0 4.212325665640282 0.0 72-73 0.0 0.0 0.0 4.6243886976996915 0.0 74-75 0.0 0.0 0.0 5.030791523274769 0.0 76-77 0.0 0.0 0.0 5.459835174787177 0.0 78-79 0.0 0.0 0.0 5.925104147799312 0.0 80-81 0.0 0.0 0.0 6.4628237638109045 0.0 82-83 0.0 0.0 0.0 7.001675421119362 0.0 84-85 0.0 0.0 0.0 7.6842963231298675 0.0 86-87 0.0 0.0 0.0 8.33522006882811 0.0 88-89 0.0 0.0 0.0 9.073310994385075 0.0 90-91 0.0 0.0 0.0 9.793289259192175 0.0 92-93 0.0 0.0 0.0 10.376290527078428 0.0 94-95 0.0 0.0 0.0 11.100796957072994 0.0 96-97 0.0 0.0 0.0 11.820775221880094 0.0 98-99 0.0 0.0 0.0 12.650561492483245 0.0 100-101 0.0 0.0 0.0 13.497328382539393 0.0 102-103 0.0 0.0 0.0 14.36220793334541 0.0 104-105 0.0 0.0 0.0 15.090110487230575 0.0 106-107 0.0 0.0 0.0 15.905180220974461 0.0 108-109 0.0 0.0 0.0 16.845906538670533 0.0 110-111 0.0 0.0 0.0 17.91908168809998 0.0 112-113 0.0 0.0 0.0 18.85980800579605 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCAACA 15 2.6850376E-4 118.99208 1 CCTTTAG 15 2.6850376E-4 118.99208 1 GTACATA 45 0.0 118.99207 1 TACATAA 45 3.947207E-10 92.549385 2 TACATGA 45 4.2093234E-8 79.32805 2 TACAAGA 30 4.2202955E-5 79.32805 2 GAACAAA 205 0.0 75.45839 1 TTTAGTG 25 0.0020442717 71.39524 5 ACTGACT 25 0.0020442717 71.39524 8 GCAACAA 25 0.0020442717 71.39524 2 CGCAGAA 25 0.0020442717 71.39524 2 TAGTGGA 25 0.0020442717 71.39524 7 GTACAAA 95 0.0 68.89015 1 GAGAACA 175 0.0 64.595695 1 GTCGGGA 175 0.0 64.595695 2 GACAATG 30 0.0042107673 59.49604 7 TCGGGAG 190 0.0 59.49604 3 AGAACAA 130 0.0 59.49604 2 GGGAACC 30 0.0042107673 59.49604 7 GTACTAG 30 0.0042107673 59.49604 1 >>END_MODULE