FastQCFastQC Report
Thu 26 May 2016
SRR936230_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936230_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275116
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24150.061.0949671
GTACATG134550.055.0941161
TACATGG136950.052.9991232
ACATGGG138050.050.422033
TATCCCG751.4720226E-647.594955
CATGGGA100850.047.4887664
ATGGGAG33900.045.1028865
ATGGGAT37750.043.497375
GTATCAA34450.042.483162
CATGGGG35000.039.7757764
TACCTGG11850.038.6583442
TCAACGC40700.035.8131525
CAACGCA41750.034.7699626
ATCAACG42200.034.6811524
AGTACAT74800.034.678142
GAGTACA80350.033.837731
AACGCAG42600.033.7968837
CGACCGT651.7142829E-632.09295318-19
CATGGGC16600.031.538824
ATGGGAC19150.031.377875
Produced by FastQC (version 0.11.5)