##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936230_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1275116 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.640624852954556 31.0 30.0 31.0 22.0 34.0 2 28.769344906659473 31.0 30.0 33.0 20.0 34.0 3 28.963158645958487 31.0 30.0 34.0 22.0 34.0 4 32.351274707556016 35.0 33.0 37.0 20.0 37.0 5 32.669451249925494 35.0 33.0 37.0 25.0 37.0 6 32.52603527835899 35.0 33.0 37.0 23.0 37.0 7 32.45953466194448 35.0 33.0 37.0 23.0 37.0 8 32.21954630010132 35.0 33.0 37.0 19.0 37.0 9 33.50846040673947 38.0 33.0 39.0 17.0 39.0 10-11 33.41502929929512 38.0 33.0 39.0 17.0 39.0 12-13 33.402342610397795 38.0 33.0 39.0 17.0 39.0 14-15 34.408848293018046 39.0 33.0 41.0 17.0 41.0 16-17 34.420507232283185 39.0 33.0 41.0 17.0 41.0 18-19 34.46751628871412 39.0 33.5 41.0 18.5 41.0 20-21 34.59537210732201 39.0 34.0 41.0 20.0 41.0 22-23 34.51063315023888 39.0 33.5 41.0 21.5 41.0 24-25 34.49610153115481 39.0 33.0 41.0 22.5 41.0 26-27 34.599485850699075 39.0 33.0 41.0 24.0 41.0 28-29 34.49449148155932 39.0 33.0 40.0 24.0 41.0 30-31 34.40862203909292 38.0 33.0 40.0 24.0 41.0 32-33 34.22490777309672 38.0 33.0 40.0 22.5 41.0 34-35 34.17560480771945 38.0 33.0 40.0 23.0 41.0 36-37 34.055592197102065 38.0 33.0 40.0 22.5 41.0 38-39 33.80857231812635 38.0 32.0 40.0 20.5 41.0 40-41 33.89902016757691 38.0 32.5 40.0 21.5 41.0 42-43 33.80397979477945 38.0 32.0 40.0 19.5 41.0 44-45 33.67771402758651 38.0 32.0 40.0 19.0 41.0 46-47 33.64776930098908 38.0 32.0 40.0 19.0 41.0 48-49 33.55553455528752 38.0 32.0 40.0 19.0 41.0 50-51 33.416915794327736 38.0 32.0 40.0 18.0 41.0 52-53 33.24814565890476 38.0 31.5 40.0 16.5 41.0 54-55 33.06747072423215 38.0 31.0 40.0 14.5 41.0 56-57 32.858883427076435 37.0 31.0 40.0 13.0 41.0 58-59 32.62274961650547 37.0 31.0 40.0 10.0 41.0 60-61 32.366723890218616 37.0 31.0 40.0 8.0 41.0 62-63 32.105367668510155 36.0 30.5 40.0 7.5 41.0 64-65 31.82336548204242 36.0 30.0 39.0 4.5 41.0 66-67 31.522635195542996 35.5 30.0 39.0 2.0 41.0 68-69 31.235591899089968 35.0 30.0 39.0 2.0 40.5 70-71 30.89683918953256 35.0 30.0 38.5 2.0 40.0 72-73 30.56641552611684 35.0 29.5 37.5 2.0 40.0 74-75 30.221348096957456 35.0 29.0 37.0 2.0 39.5 76-77 29.889075190021927 34.0 29.0 36.5 2.0 39.0 78-79 29.54513510927633 34.0 29.0 36.0 2.0 39.0 80-81 29.229712041884817 34.0 29.0 36.0 2.0 37.5 82-83 28.908842411200236 34.0 29.0 35.0 2.0 37.0 84-85 28.9207993625678 34.0 29.0 35.0 2.0 37.0 86-87 28.77700068072238 34.0 29.0 35.0 2.0 36.0 88-89 28.5869438545199 34.0 29.0 35.0 2.0 36.0 90-91 28.39997223781993 34.0 29.0 35.0 2.0 36.0 92-93 28.25996654422029 34.0 29.0 35.0 2.0 35.5 94-95 28.12567954601777 34.0 28.0 35.0 2.0 35.0 96-97 28.010080651485826 34.0 28.0 35.0 2.0 35.0 98-99 27.875068621207795 34.0 28.0 35.0 2.0 35.0 100-101 27.761054288394156 34.0 27.0 35.0 2.0 35.0 102-103 26.808913463559392 32.5 25.5 34.0 2.0 35.0 104-105 27.4479737529762 34.0 27.0 35.0 2.0 35.0 106-107 27.55772886545224 34.0 27.0 35.0 2.0 35.0 108-109 27.502827585882383 34.0 27.0 35.0 2.0 35.0 110-111 27.379629774859698 34.0 27.0 35.0 2.0 35.0 112-113 27.32900889017156 34.0 27.0 35.0 2.0 35.0 114-115 27.209262137719236 34.0 26.5 35.0 2.0 35.0 116-117 27.065850871606976 34.0 25.5 35.0 2.0 35.0 118-119 26.847098616910145 34.0 25.0 35.0 2.0 35.0 120-121 26.617781833182235 34.0 25.0 35.0 2.0 35.0 122-123 26.28420041784434 34.0 25.0 35.0 2.0 35.0 124-125 24.307410070926878 31.5 13.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6335082802767289 1101 2 0.8115507370209301 1101 3 0.8662152209494778 1101 4 0.8148272766091011 1101 5 0.948125518865595 1101 6 0.9294420763911191 1101 7 0.7338429921539884 1101 8 0.8052997004718279 1101 9 0.9577471082658491 1101 10-11 0.9586983766604362 1101 12-13 0.9262112587026863 1101 14-15 0.9664632115511935 1101 16-17 1.0587091942368758 1101 18-19 1.057794616150666 1101 20-21 0.9290765747651193 1101 22-23 1.1574032252694337 1101 24-25 1.321288698789516 1101 26-27 1.2581202259119237 1101 28-29 1.2848217412473133 1101 30-31 1.2231531720573585 1101 32-33 1.449173845207163 1101 34-35 1.335865756273023 1101 36-37 1.1573133331568783 1101 38-39 1.3458814875888976 1101 40-41 1.3261497229730708 1101 42-43 1.4057390863438712 1101 44-45 1.29635552748735 1101 46-47 1.2697600219743919 1101 48-49 1.2525236538717976 1101 50-51 1.1554169168979627 1101 52-53 1.176546659048718 1101 54-55 1.2358131351749364 1101 56-57 1.296618160958488 1101 58-59 1.1784251699601285 1101 60-61 1.1178043170951355 1101 62-63 1.0528047518488393 1101 64-65 1.0644653666729198 1101 66-67 1.026035585151746 1101 68-69 1.0497429455536569 1101 70-71 0.9723144822724556 1101 72-73 0.9568454531340187 1101 74-75 0.8935812478698253 1101 76-77 0.9104282217047732 1101 78-79 0.926025595918599 1101 80-81 0.9998633488807229 1101 82-83 0.8908547258894473 1101 84-85 0.7616570422651243 1101 86-87 1.0521820585321713 1101 88-89 1.0191070274729483 1101 90-91 1.0310696534932475 1101 92-93 0.8614267626508436 1101 94-95 0.9777178621974052 1101 96-97 0.9298506701947211 1101 98-99 0.8958826336975036 1101 100-101 0.9575189124033727 1101 102-103 1.0155403642653589 1101 104-105 0.9406919885531586 1101 106-107 0.8883769324638742 1101 108-109 0.810746905092433 1101 110-111 0.8776539732723592 1101 112-113 0.8316338115752302 1101 114-115 0.91276425538463 1101 116-117 0.8544670603880924 1101 118-119 0.8057488493084435 1101 120-121 0.8948380621246663 1101 122-123 1.0093833281373783 1101 124-125 0.7835178322047263 1102 1 0.6270993178803579 1102 2 0.6693232188558582 1102 3 0.7531134146510219 1102 4 0.8946446973131899 1102 5 0.74316168751497 1102 6 0.6645431421291192 1102 7 0.8109378727126639 1102 8 0.7792661168628996 1102 9 0.8944900957236186 1102 10-11 1.0403811560922378 1102 12-13 0.938714156924064 1102 14-15 1.087612441402932 1102 16-17 1.0031001409430544 1102 18-19 0.9971920860178116 1102 20-21 0.8398074341036192 1102 22-23 1.0568617625356538 1102 24-25 1.1365998437628733 1102 26-27 1.0542067907380641 1102 28-29 1.0469344610074671 1102 30-31 0.986994673664789 1102 32-33 1.0101763010532707 1102 34-35 0.906360189957681 1102 36-37 0.9588650495383817 1102 38-39 1.0542605484152148 1102 40-41 0.8985502346749641 1102 42-43 1.0585794586542931 1102 44-45 1.2939144464536625 1102 46-47 1.030341904082242 1102 48-49 0.9974213423311724 1102 50-51 0.9418242874354235 1102 52-53 0.9837099087940757 1102 54-55 0.9134509780446152 1102 56-57 0.9865374260272262 1102 58-59 1.1199125890838886 1102 60-61 1.0871122391309598 1102 62-63 1.0927216521996215 1102 64-65 1.0433471665347298 1102 66-67 1.0013769985148215 1102 68-69 1.0100033413728333 1102 70-71 0.9427003696357481 1102 72-73 0.9015774959850162 1102 74-75 0.7081843616842782 1102 76-77 0.7644790988997769 1102 78-79 0.7093606787587028 1102 80-81 0.7719137669743894 1102 82-83 0.8746005775397308 1102 84-85 0.8730757461459149 1102 86-87 0.8427591765985021 1102 88-89 0.906566755172797 1102 90-91 0.9000797364198689 1102 92-93 0.9562148089439475 1102 94-95 0.922847177663467 1102 96-97 1.0915371639639808 1102 98-99 1.0091978413716483 1102 100-101 1.0393977903966167 1102 102-103 1.0785974716270097 1102 104-105 1.0544186571500482 1102 106-107 1.0028487755942486 1102 108-109 0.9491179720185663 1102 110-111 1.0677048550814519 1102 112-113 1.1725160936087953 1102 114-115 1.1372112862793848 1102 116-117 1.1463541168448685 1102 118-119 1.2242430199903254 1102 120-121 1.3632620410567604 1102 122-123 1.3118871607107288 1102 124-125 1.265917640258838 1103 1 0.695902256716991 1103 2 0.6310083702433822 1103 3 0.716394335611291 1103 4 0.6722875444281655 1103 5 0.7007193645237209 1103 6 0.6448066078972872 1103 7 0.5088448875472338 1103 8 0.729614752737298 1103 9 0.7856162597930663 1103 10-11 0.9429906605145248 1103 12-13 0.6898214852969389 1103 14-15 0.8918323393859922 1103 16-17 0.7520355146151942 1103 18-19 0.8996857684549937 1103 20-21 0.8109496260639375 1103 22-23 0.9393205813853882 1103 24-25 0.9283168169483247 1103 26-27 0.9629045538528658 1103 28-29 0.9027957554058972 1103 30-31 0.9121578773710937 1103 32-33 1.069635681464142 1103 34-35 1.0029492909918858 1103 36-37 0.9464861361591801 1103 38-39 1.087989899344123 1103 40-41 1.0570567160264588 1103 42-43 1.180233490195313 1103 44-45 1.176018194874267 1103 46-47 1.1191726869920657 1103 48-49 1.0825715599361132 1103 50-51 0.9599007065472165 1103 52-53 0.9401269147372631 1103 54-55 1.0719106626344441 1103 56-57 1.0449204809198278 1103 58-59 0.9994834627794305 1103 60-61 1.1254814889788705 1103 62-63 1.0877377970824043 1103 64-65 0.9687835463372494 1103 66-67 1.1244355178652867 1103 68-69 1.0267203145582862 1103 70-71 1.1309344938343564 1103 72-73 1.0831992377604855 1103 74-75 1.0218577904830006 1103 76-77 0.9550858189147533 1103 78-79 0.8158724481442619 1103 80-81 0.8274100466318366 1103 82-83 0.8616507436682355 1103 84-85 1.0082493621316928 1103 86-87 0.8877870206716914 1103 88-89 0.9058601240716655 1103 90-91 0.8441219237005058 1103 92-93 0.7852984113586601 1103 94-95 0.8488843108938475 1103 96-97 0.9687974475523582 1103 98-99 0.8589960303369715 1103 100-101 0.9048407217457672 1103 102-103 0.9188485362533392 1103 104-105 0.9089807564527739 1103 106-107 0.8643151521209944 1103 108-109 0.8925769546569668 1103 110-111 0.7933488955138515 1103 112-113 0.9273671629147984 1103 114-115 0.9853645690722139 1103 116-117 0.9792955272992643 1103 118-119 0.8884107395841312 1103 120-121 0.8781978125013801 1103 122-123 0.9459195206714206 1103 124-125 1.0516984442272062 1104 1 0.33718342938023227 1104 2 0.2538714960762398 1104 3 0.4008032448060348 1104 4 0.27177355242359624 1104 5 0.3479685554622307 1104 6 0.29553000405186935 1104 7 0.10595606211415287 1104 8 0.06946816983229098 1104 9 0.4252174400710018 1104 10-11 0.5369726707943627 1104 12-13 0.469597327672723 1104 14-15 0.4063573727002847 1104 16-17 0.4085413208211719 1104 18-19 0.36264265023682896 1104 20-21 0.3234972179162128 1104 22-23 0.54062221854511 1104 24-25 0.5291734702892796 1104 26-27 0.36500573413079707 1104 28-29 0.5507826200546688 1104 30-31 0.3799262954179241 1104 32-33 0.6315169873845718 1104 34-35 0.47451077966502453 1104 36-37 0.45633764958008527 1104 38-39 0.6528552144021873 1104 40-41 0.44978468099885305 1104 42-43 0.6508883540826176 1104 44-45 0.5775173382209289 1104 46-47 0.43100402146760075 1104 48-49 0.4634824679886549 1104 50-51 0.5292087210151379 1104 52-53 0.42418364422517385 1104 54-55 0.43138144123714994 1104 56-57 0.5836340731528367 1104 58-59 0.4697480734780797 1104 60-61 0.42634052191243654 1104 62-63 0.4703620118168388 1104 64-65 0.4242708869045373 1104 66-67 0.5198357653429184 1104 68-69 0.476363375666228 1104 70-71 0.41436777005746706 1104 72-73 0.4718580993633772 1104 74-75 0.417772029965203 1104 76-77 0.40630892951821806 1104 78-79 0.33239633925410317 1104 80-81 0.3947287216746709 1104 82-83 0.3358131270681959 1104 84-85 0.3924393488059721 1104 86-87 0.3539682504451527 1104 88-89 0.3720746681417211 1104 90-91 0.43840376264967773 1104 92-93 0.27469732626443033 1104 94-95 0.44479379119082196 1104 96-97 0.400507898723113 1104 98-99 0.4007079092632999 1104 100-101 0.4335957188902597 1104 102-103 0.4371713993791708 1104 104-105 0.5247693534449702 1104 106-107 0.5163853976949504 1104 108-109 0.39398091429907467 1104 110-111 0.49368441807239094 1104 112-113 0.2956305362252891 1104 114-115 0.2399310072979901 1104 116-117 0.38381675326537845 1104 118-119 0.39658701980495437 1104 120-121 0.4502780523643146 1104 122-123 0.4287222048518906 1104 124-125 0.36320139492204717 1105 1 0.4356656039163376 1105 2 0.5244722674301627 1105 3 0.6305167287545927 1105 4 0.5905613655845343 1105 5 0.49963567523405317 1105 6 0.5723064450414483 1105 7 0.7074752699327505 1105 8 0.4926325179829334 1105 9 0.6108833433706806 1105 10-11 0.7227224369460288 1105 12-13 0.6219207203397943 1105 14-15 0.6304712833356589 1105 16-17 0.7599242515052964 1105 18-19 0.7680723091177626 1105 20-21 0.4845926879398519 1105 22-23 0.4226891613755299 1105 24-25 0.6969033213175209 1105 26-27 0.6015259326369318 1105 28-29 0.6585538726062623 1105 30-31 0.6685626485819043 1105 32-33 0.7344756872338962 1105 34-35 0.7931703794272593 1105 36-37 0.7230928253464839 1105 38-39 0.8058876398356745 1105 40-41 0.7318268566408364 1105 42-43 0.7443835688314167 1105 44-45 0.8745685452469729 1105 46-47 0.8269672001461643 1105 48-49 0.9203580698889269 1105 50-51 0.772332888722417 1105 52-53 0.7236107733970059 1105 54-55 0.771641485196028 1105 56-57 0.8009895364490092 1105 58-59 0.8122248383994517 1105 60-61 0.8701792518049345 1105 62-63 0.8155331012505727 1105 64-65 0.8202753058317427 1105 66-67 0.8340734428027048 1105 68-69 0.7761919636830577 1105 70-71 0.7156867668365798 1105 72-73 0.7707098233900886 1105 74-75 0.7148830238321544 1105 76-77 0.7929076918499725 1105 78-79 0.7516490843801513 1105 80-81 0.7788771450991341 1105 82-83 0.6689571546047013 1105 84-85 0.6930951609163216 1105 86-87 0.6778044229814526 1105 88-89 0.701076815399361 1105 90-91 0.8356567934623662 1105 92-93 0.7747938607011875 1105 94-95 0.7172261825262218 1105 96-97 0.74854771975426 1105 98-99 0.8241259195019417 1105 100-101 0.795556542957975 1105 102-103 0.7569695986317804 1105 104-105 0.7294872148500886 1105 106-107 0.9284284718674982 1105 108-109 0.8611050635749535 1105 110-111 0.843700315151473 1105 112-113 0.8092658526232412 1105 114-115 0.8405914272573476 1105 116-117 0.8150221435271519 1105 118-119 0.8587830745646343 1105 120-121 0.9616343266419172 1105 122-123 0.9190312072065012 1105 124-125 0.7679807711114499 1106 1 -0.3068585086060871 1106 2 -0.36215262296746076 1106 3 -0.1467519398480448 1106 4 -0.2638255448753526 1106 5 -0.2768028307950843 1106 6 -0.2677295930237591 1106 7 -0.4454837930775568 1106 8 -0.4919966036696728 1106 9 -0.28304086407343476 1106 10-11 -0.2774070748508066 1106 12-13 -0.35961057566303367 1106 14-15 -0.4335783333576799 1106 16-17 -0.35734586505531496 1106 18-19 -0.4402711465143838 1106 20-21 -0.4982827957183389 1106 22-23 -0.5183178129553951 1106 24-25 -4.412261448256057 1106 26-27 -1.4654075336940195 1106 28-29 -1.060428856472754 1106 30-31 -0.9245836836600958 1106 32-33 -5.915851309942589 1106 34-35 -1.6081786347546796 1106 36-37 -0.9517014439576101 1106 38-39 -0.4027349499176154 1106 40-41 -0.8419023591290227 1106 42-43 -0.5575092340130183 1106 44-45 -0.5366402583375205 1106 46-47 -0.6626235908411076 1106 48-49 -0.38251532227439355 1106 50-51 -0.20016314950368042 1106 52-53 -0.19797425793519352 1106 54-55 -0.30048049721932557 1106 56-57 -0.1510540953513555 1106 58-59 -0.055634516615711505 1106 60-61 -0.153229402157244 1106 62-63 -0.15517631771606233 1106 64-65 -0.133877518088525 1106 66-67 -0.3070871759580065 1106 68-69 -0.20544390706256266 1106 70-71 -0.06817413266709238 1106 72-73 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28-29 0.835156031187033 2203 30-31 0.8422425920549657 2203 32-33 1.0225249044850244 2203 34-35 1.0644310033775923 2203 36-37 0.9815785521779574 2203 38-39 0.9212993952123014 2203 40-41 1.015206684243168 2203 42-43 1.067721877071257 2203 44-45 1.0084848224230427 2203 46-47 0.8111791903415337 2203 48-49 0.7923302514426425 2203 50-51 0.7577284411323291 2203 52-53 0.7108752888998993 2203 54-55 0.6282612078400547 2203 56-57 0.6175202315432671 2203 58-59 0.5630475916728841 2203 60-61 0.5859070182928434 2203 62-63 0.477645381063617 2203 64-65 0.6870344876115126 2203 66-67 0.7425562699067498 2203 68-69 0.6271127159078524 2203 70-71 0.6776791517972889 2203 72-73 0.6281542521852082 2203 74-75 0.6533935344564021 2203 76-77 0.571215837006779 2203 78-79 0.6710751277198348 2203 80-81 0.6286275910061967 2203 82-83 0.6455133909715443 2203 84-85 0.649955146689905 2203 86-87 0.6594412185104268 2203 88-89 0.599586592215033 2203 90-91 0.7042161733704511 2203 92-93 0.759409090543052 2203 94-95 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2216 100-101 -1.4638350720431461 2216 102-103 -1.5673843987352534 2216 104-105 -1.5370817217929584 2216 106-107 -1.62830096730508 2216 108-109 -1.7231702317915776 2216 110-111 -1.4986373171171579 2216 112-113 -1.6396480554013237 2216 114-115 -1.5779214849943841 2216 116-117 -1.580642787277057 2216 118-119 -1.8493469275853016 2216 120-121 -1.887790604561701 2216 122-123 -1.6373205110414624 2216 124-125 -1.5189381338754337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50298.0 3 8333.0 4 5313.0 5 4534.0 6 5061.0 7 5449.0 8 5851.0 9 6526.0 10 7644.0 11 8619.0 12 9166.0 13 9141.0 14 9133.0 15 8987.0 16 8291.0 17 7657.0 18 6729.0 19 6256.0 20 5973.0 21 6105.0 22 7022.0 23 9601.0 24 13956.0 25 20426.0 26 23041.0 27 21823.0 28 24352.0 29 29762.0 30 34427.0 31 41680.0 32 52060.0 33 66455.0 34 89757.0 35 126216.0 36 194929.0 37 252360.0 38 81967.0 39 216.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.16319011476621 17.85401148446335 9.363868642333156 21.618929758437286 2 18.96495610199864 21.165463507213076 37.69338451825914 22.176195872529146 3 17.072564378456548 30.06502937771936 29.716433642115696 23.145972601708394 4 10.587618969941463 16.465847705927008 36.70705004736839 36.23948327676314 5 10.569073811060562 43.995601937719734 34.04867199380131 11.386652257418397 6 29.728485531018183 37.608608532460416 19.452014615244053 13.210891321277352 7 28.419609617687584 35.44889103598202 20.94691512647154 15.184584219858852 8 27.7786753080533 34.2421480914498 19.758858885236027 18.220317715260872 9 28.47529007642568 14.413749563757994 18.15833330065603 38.9526270591603 10-11 23.85940050051094 28.37764499485137 28.42552370525536 19.33743079938232 12-13 24.55490379302786 27.261615753247128 27.887728665321138 20.295751788403873 14-15 22.512060018147867 28.204247416438644 25.13797162690057 24.145720938512927 16-17 22.19394517372216 30.465962189098427 25.87774747035877 21.462345166820644 18-19 21.66885736513872 29.110814312423926 27.610251214033855 21.610077108403498 20-21 23.439225993966655 28.209356193589347 27.14122755386817 21.21019025857583 22-23 23.01088697507405 28.536402623800633 26.758472738604667 21.69423766252065 24-25 22.239118619769098 28.36295605568544 27.295071489015292 22.10285383553017 26-27 22.277211416525304 28.338779969241607 27.123344932432698 22.260663681800395 28-29 22.193945572896244 28.345227825268605 27.223511881421064 22.237314720414087 30-31 22.390857836582615 28.199565054744596 27.47036906038179 21.939208048291 32-33 21.96473834126999 28.487776606624127 27.241595926894917 22.305889125210964 34-35 22.41858918506447 28.400087208367612 27.298728655712818 21.882594950855104 36-37 22.484033749205462 28.114011990323984 27.074808752018942 22.327145508451615 38-39 22.23492704334893 28.301871255459837 27.227725684089915 22.235476017101316 40-41 22.2337114991585 28.235741140708743 27.457058337293798 22.073489022838956 42-43 22.13869949227591 28.12700474158146 27.37145734223615 22.36283842390648 44-45 22.07256064038949 28.238199543721702 27.441869219566478 22.247370596322323 46-47 22.32421442281865 28.2775238756252 27.05710739766959 22.341154303886565 48-49 22.17558586918503 28.282922154411295 27.126711807728565 22.414780168675115 50-51 22.296320956661848 28.162368378021966 27.363686234928146 22.177624430388036 52-53 22.2847357641725 28.108350590129493 27.359312180246043 22.247601465451964 54-55 22.027644890596815 28.27668418163281 27.35793271115991 22.33773821661046 56-57 22.252324424623783 28.515634649974647 27.1040204281937 22.128020497207867 58-59 22.348547083285133 28.076181812121725 27.105714616218634 22.469556488374508 60-61 22.296996050127657 28.357283657927645 27.278705392381564 22.067014899563134 62-63 21.922019541189467 28.297315939162495 27.50275174172409 22.27791277792395 64-65 21.91454821367652 28.346567537575336 27.40299818446324 22.335886064284903 66-67 21.97554255975745 28.62981555360192 26.953101411404322 22.441540475236312 68-69 22.08865246558347 28.353935412833675 27.214037333132566 22.343374788450287 70-71 21.946751218915402 28.19472688217243 27.369032765042046 22.489489133870126 72-73 22.099335509872457 28.388660455975273 27.02282072255557 22.489183311596697 74-75 21.81445879698505 28.569977719621715 27.225587065616928 22.389976417776307 76-77 21.617025314619177 28.554684957333194 27.413139070811766 22.41515065723587 78-79 22.018218756041126 28.356394661028478 27.170814856234482 22.454571726695917 80-81 21.84144536266408 28.55548039878834 26.954288347106626 22.64878589144095 82-83 22.00856393344914 28.451708277292635 27.047129082474914 22.49259870678331 84-85 21.76617149525454 28.153286686523714 27.309362143306405 22.77117967491534 86-87 21.527957500270563 28.362052334144238 27.425939955172634 22.68405021041256 88-89 21.93231350294954 28.108898498487196 27.388727596120816 22.57006040244245 90-91 21.93683150558754 28.26701309334424 27.234477558272264 22.561677842795955 92-93 21.730244617248097 28.311614368748845 27.35997433955931 22.598166674443746 94-95 21.550543716091266 28.09380180909315 27.469151777801827 22.886502697013757 96-97 21.74176788322599 28.265741258124283 27.4109147232107 22.58157613543903 98-99 21.52032254446868 28.2572654459102 27.274723533872734 22.947688475748386 100-101 21.288365257326415 28.357352138478813 27.443393207812335 22.910889396382437 102-103 21.434153590971743 28.12488358824648 27.404003191887742 23.036959628894035 104-105 21.267308283507642 28.227363557083613 27.558677198551973 22.946650960856772 106-107 21.12542403187802 28.29386159081899 27.628193791832157 22.95252058547083 108-109 21.38122255382773 28.47147637124498 27.135826374127088 23.011474700800207 110-111 21.27998971064321 28.57613967174442 27.12225826285542 23.021612354756947 112-113 21.162273005501078 28.499580625567845 27.397953201813014 22.940193167118064 114-115 20.91168426315325 28.68613553706045 27.228969992843773 23.173210206942525 116-117 20.889722458259335 28.567126406403236 27.431338034382254 23.111813100955175 118-119 20.870951297937417 28.444474943141714 27.323464826288134 23.361108932632735 120-121 20.897917858561506 28.496034249715024 27.286761762065964 23.319286129657506 122-123 20.92258067551925 28.24172540678907 27.311333628210317 23.52436028948136 124-125 20.99715044974428 28.38629659242099 26.958502125497063 23.658050832337672 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 187.0 1 256.5 2 359.0 3 383.0 4 331.0 5 274.0 6 270.0 7 275.5 8 263.0 9 274.0 10 291.0 11 286.5 12 309.5 13 337.0 14 391.5 15 452.0 16 508.0 17 623.5 18 774.0 19 964.5 20 1298.0 21 1871.5 22 2605.0 23 3597.5 24 4928.0 25 6531.0 26 8642.0 27 10773.0 28 12265.5 29 14187.5 30 17405.5 31 20845.0 32 24001.0 33 27643.5 34 31169.0 35 33054.0 36 36324.5 37 40438.5 38 41160.0 39 40783.5 40 40788.0 41 41189.0 42 41034.5 43 41550.0 44 44240.5 45 46093.0 46 46976.5 47 46769.5 48 45737.5 49 45188.5 50 44873.5 51 43280.5 52 40813.0 53 39717.0 54 38033.5 55 34848.0 56 31696.0 57 28620.5 58 26212.5 59 23736.0 60 20224.0 61 16970.5 62 14217.5 63 11060.0 64 8345.0 65 6458.5 66 4709.5 67 3496.5 68 2624.5 69 1877.0 70 1418.5 71 1127.0 72 990.5 73 858.0 74 635.0 75 459.5 76 304.0 77 193.5 78 150.0 79 120.5 80 73.5 81 38.0 82 25.5 83 19.5 84 17.0 85 14.5 86 11.0 87 7.0 88 4.5 89 3.5 90 3.5 91 3.0 92 2.0 93 1.0 94 2.5 95 3.0 96 2.5 97 2.5 98 2.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.5684847496227794E-4 2 7.842423748113897E-5 3 0.0 4 3.1369694992455587E-4 5 7.058181373302508E-4 6 7.058181373302508E-4 7 5.489696623679728E-4 8 9.410908497736677E-4 9 0.0016469089871039184 10-11 0.0014900605121416404 12-13 0.002078242293250183 14-15 0.002744848311839864 16-17 0.002666424074358725 18-19 0.0021958786494718913 20-21 0.0027840604305804334 22-23 0.09116817607182406 24-25 0.001960605937028474 26-27 0.0013332120371793625 28-29 0.0012547877996982235 30-31 0.0010195150872548066 32-33 0.0015684847496227796 34-35 7.058181373302508E-4 36-37 3.529090686651254E-4 38-39 7.450302560708202E-4 40-41 0.0010979393247359456 42-43 0.0010979393247359456 44-45 0.0013332120371793625 46-47 0.001450848393401071 48-49 1.5684847496227794E-4 50-51 0.001215575680957654 52-53 0.001137151443476515 54-55 0.0012547877996982235 56-57 0.001215575680957654 58-59 6.666060185896813E-4 60-61 9.018787310330982E-4 62-63 0.001137151443476515 64-65 8.626666122925287E-4 66-67 2.3527271244341692E-4 68-69 1.5684847496227794E-4 70-71 5.489696623679728E-4 72-73 2.3527271244341692E-4 74-75 2.3527271244341692E-4 76-77 2.3527271244341692E-4 78-79 1.1763635622170846E-4 80-81 2.744848311839864E-4 82-83 7.842423748113897E-5 84-85 1.5684847496227794E-4 86-87 1.5684847496227794E-4 88-89 1.5684847496227794E-4 90-91 1.9606059370284745E-4 92-93 1.1763635622170846E-4 94-95 1.5684847496227794E-4 96-97 2.744848311839864E-4 98-99 3.921211874056949E-4 100-101 3.1369694992455587E-4 102-103 6.666060185896813E-4 104-105 3.1369694992455587E-4 106-107 4.3133330614626437E-4 108-109 5.097575436274033E-4 110-111 9.410908497736677E-4 112-113 1.9606059370284745E-4 114-115 2.744848311839864E-4 116-117 8.234544935519592E-4 118-119 0.0012547877996982235 120-121 8.234544935519592E-4 122-123 6.273938998491117E-4 124-125 1.9606059370284745E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1275116.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.22762171162402 #Duplication Level Percentage of deduplicated Percentage of total 1 59.60059050355614 23.37989418062911 2 13.658974527370832 10.716181714568227 3 9.025924866316364 10.621966987601967 4 5.869706871584524 9.210185628665512 5 3.73597422753537 7.327669186106711 6 2.3732335202970947 5.585778406053612 7 1.440907991659858 3.9566375532662166 8 0.9560741658135856 3.000361256383346 9 0.6563993106115964 2.3174085463598244 >10 2.4954794385673558 16.550532126165784 >50 0.1324245642869087 3.5682253151954564 >100 0.05350839036316414 3.5731829415778105 >500 8.016220372311486E-4 0.1919761574266108 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.1369694992455587E-4 0.0 3 0.0 0.0 0.0 3.1369694992455587E-4 0.0 4 0.0 0.0 0.0 3.1369694992455587E-4 0.0 5 0.0 0.0 0.0 3.1369694992455587E-4 0.0 6 0.0 0.0 0.0 3.1369694992455587E-4 0.0 7 0.0 0.0 0.0 3.1369694992455587E-4 0.0 8 0.0 0.0 0.0 3.1369694992455587E-4 0.0 9 0.0 0.0 0.0 3.1369694992455587E-4 0.0 10-11 0.0 0.0 0.0 3.1369694992455587E-4 0.0 12-13 0.0 0.0 0.0 3.1369694992455587E-4 0.0 14-15 0.0 0.0 0.0 3.1369694992455587E-4 0.0 16-17 0.0 0.0 0.0 3.1369694992455587E-4 3.9212118740569484E-5 18-19 0.0 0.0 0.0 3.1369694992455587E-4 7.842423748113897E-5 20-21 0.0 0.0 0.0 3.1369694992455587E-4 7.842423748113897E-5 22-23 0.0 0.0 0.0 4.7054542488683383E-4 7.842423748113897E-5 24-25 0.0 0.0 0.0 7.058181373302507E-4 7.842423748113897E-5 26-27 0.0 0.0 0.0 8.626666122925286E-4 7.842423748113897E-5 28-29 0.0 0.0 0.0 0.0012547877996982235 7.842423748113897E-5 30-31 0.0 0.0 0.0 0.003646727042872962 7.842423748113897E-5 32-33 0.0 0.0 0.0 0.009528544853958385 7.842423748113897E-5 34-35 0.0 0.0 0.0 0.018821816995473355 7.842423748113897E-5 36-37 0.0 0.0 0.0 0.034192967541776594 7.842423748113897E-5 38-39 0.0 0.0 0.0 0.05779866302359942 2.744848311839864E-4 40-41 0.0 0.0 0.0 0.08399235834229984 4.7054542488683383E-4 42-43 0.0 0.0 0.0 0.11928326520881237 4.7054542488683383E-4 44-45 0.0 0.0 0.0 0.1606520504801132 4.7054542488683383E-4 46-47 0.0 0.0 0.0 0.2110004109430044 4.7054542488683383E-4 48-49 0.0 0.0 0.0 0.2678971952355707 4.7054542488683383E-4 50-51 0.0 0.0 0.0 0.3220491312162972 4.7054542488683383E-4 52-53 0.0 0.0 0.0 0.38286712738292045 4.7054542488683383E-4 54-55 0.0 0.0 0.0 0.4593699710457715 4.7054542488683383E-4 56-57 0.0 0.0 0.0 0.5583021466282283 4.7054542488683383E-4 58-59 0.0 0.0 0.0 0.6722133515695827 4.7054542488683383E-4 60-61 0.0 0.0 0.0 0.7803603750560734 4.7054542488683383E-4 62-63 0.0 0.0 0.0 0.8925854588915831 4.7054542488683383E-4 64-65 0.0 0.0 0.0 1.0231618142976795 4.7054542488683383E-4 66-67 0.0 0.0 0.0 1.1793436832413677 4.7054542488683383E-4 68-69 0.0 0.0 0.0 1.3801097311930837 4.7054542488683383E-4 70-71 0.0 0.0 0.0 1.5656614770734585 4.7054542488683383E-4 72-73 0.0 0.0 0.0 1.7475664959109602 4.7054542488683383E-4 74-75 0.0 0.0 0.0 1.9506460588683696 4.7054542488683383E-4 76-77 0.0 0.0 0.0 2.2024270733015663 4.7054542488683383E-4 78-79 0.0 0.0 0.0 2.4969492971619838 4.7054542488683383E-4 80-81 0.0 0.0 0.0 2.801627459776209 4.7054542488683383E-4 82-83 0.0 3.9212118740569484E-5 0.0 3.112344288676481 4.7054542488683383E-4 84-85 0.0 7.842423748113897E-5 0.0 3.453921055025582 4.7054542488683383E-4 86-87 0.0 7.842423748113897E-5 0.0 3.824710849836407 4.7054542488683383E-4 88-89 0.0 7.842423748113897E-5 0.0 4.257142095307407 4.7054542488683383E-4 90-91 0.0 7.842423748113897E-5 0.0 4.700984067331913 4.7054542488683383E-4 92-93 0.0 7.842423748113897E-5 0.0 5.142394887994504 4.7054542488683383E-4 94-95 0.0 7.842423748113897E-5 0.0 5.612273706862748 4.7054542488683383E-4 96-97 0.0 7.842423748113897E-5 0.0 6.1170513114100995 4.7054542488683383E-4 98-99 0.0 7.842423748113897E-5 0.0 6.685038851367248 4.7054542488683383E-4 100-101 0.0 7.842423748113897E-5 0.0 7.305060872893133 4.7054542488683383E-4 102-103 0.0 7.842423748113897E-5 0.0 7.92178907644481 4.7054542488683383E-4 104-105 0.0 7.842423748113897E-5 0.0 8.541615037376992 4.7054542488683383E-4 106-107 0.0 7.842423748113897E-5 0.0 9.229513236442802 4.7054542488683383E-4 108-109 0.0 7.842423748113897E-5 0.0 9.941605312771543 4.7054542488683383E-4 110-111 0.0 7.842423748113897E-5 0.0 10.70357520413829 4.7054542488683383E-4 112-113 0.0 7.842423748113897E-5 0.0 11.468015458985692 4.7054542488683383E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2415 0.0 61.094967 1 GTACATG 13455 0.0 55.094116 1 TACATGG 13695 0.0 52.999123 2 ACATGGG 13805 0.0 50.42203 3 TATCCCG 75 1.4720226E-6 47.59495 5 CATGGGA 10085 0.0 47.488766 4 ATGGGAG 3390 0.0 45.102886 5 ATGGGAT 3775 0.0 43.49737 5 GTATCAA 3445 0.0 42.48316 2 CATGGGG 3500 0.0 39.775776 4 TACCTGG 1185 0.0 38.658344 2 TCAACGC 4070 0.0 35.813152 5 CAACGCA 4175 0.0 34.769962 6 ATCAACG 4220 0.0 34.681152 4 AGTACAT 7480 0.0 34.67814 2 GAGTACA 8035 0.0 33.83773 1 AACGCAG 4260 0.0 33.796883 7 CGACCGT 65 1.7142829E-6 32.092953 18-19 CATGGGC 1660 0.0 31.53882 4 ATGGGAC 1915 0.0 31.37787 5 >>END_MODULE