FastQCFastQC Report
Thu 26 May 2016
SRR936230_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936230_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275116
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56620.4440380326182088No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38280.3002079810778No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37420.293463496654422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43650.080.556191
GTATCAA65700.053.1010442
GTACATG114200.048.429841
TACATGG116250.047.3205532
ACATGGG116800.046.5370373
CATGGGA87250.042.987874
CATGGGG28850.039.208354
ATCAACG91300.039.124724
TCAACGC91800.038.8467755
CAACGCA93250.038.279536
ATGGGAG29550.038.0780875
AACGCAG95050.037.8675657
TATCAAC96250.037.4218643
ATGGGAT35500.036.8947945
ATGGGAC16050.035.238665
ACGCAGA112150.031.8284958
CGCAGAG113150.031.4420459
TGGGATA11850.029.620876
TACCTGG9800.029.1598952
CGTTTAA3550.028.5398521
Produced by FastQC (version 0.11.5)