##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936230_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1275116 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.54426185539198 31.0 31.0 34.0 30.0 34.0 2 31.31491644681739 31.0 31.0 34.0 28.0 34.0 3 31.67777755121887 31.0 31.0 34.0 30.0 34.0 4 35.542469861565536 37.0 35.0 37.0 33.0 37.0 5 35.216160725769264 37.0 35.0 37.0 33.0 37.0 6 35.42139930798453 37.0 35.0 37.0 33.0 37.0 7 35.23380774768727 37.0 35.0 37.0 32.0 37.0 8 35.31719702364334 37.0 35.0 37.0 32.0 37.0 9 36.95515858949303 39.0 37.0 39.0 33.0 39.0 10-11 36.8584736604356 39.0 37.0 39.0 32.0 39.0 12-13 36.818802759905765 39.0 37.0 39.0 32.0 39.0 14-15 37.947650645117776 40.0 37.0 41.0 32.5 41.0 16-17 37.86805514164986 40.0 37.0 41.0 32.0 41.0 18-19 37.9998690315234 40.0 37.0 41.0 33.0 41.0 20-21 38.049862914432886 40.0 37.0 41.0 33.0 41.0 22-23 37.96130704971156 40.0 37.0 41.0 32.5 41.0 24-25 37.93113528494662 40.0 37.0 41.0 33.0 41.0 26-27 37.757395013473285 40.0 37.0 41.0 32.0 41.0 28-29 37.603746639521425 40.0 37.0 41.0 32.0 41.0 30-31 37.39361516912971 39.5 36.0 41.0 32.0 41.0 32-33 37.15729157190405 39.0 36.0 41.0 31.0 41.0 34-35 36.927239560949744 39.0 35.0 41.0 31.0 41.0 36-37 36.69926069471327 39.0 35.0 41.0 30.0 41.0 38-39 36.65772290521019 39.0 35.0 41.0 30.5 41.0 40-41 36.65843813425602 39.0 35.0 41.0 30.0 41.0 42-43 36.52063263263891 39.0 35.0 41.0 30.0 41.0 44-45 36.298610087239126 39.0 35.0 41.0 30.0 41.0 46-47 35.93517962287353 39.0 35.0 41.0 28.5 41.0 48-49 35.5916920499782 39.0 35.0 41.0 27.0 41.0 50-51 35.38150607474144 39.0 35.0 41.0 26.0 41.0 52-53 35.22732794506539 39.0 35.0 41.0 26.0 41.0 54-55 35.048570090878 39.0 35.0 41.0 26.0 41.0 56-57 34.858910483438365 38.5 34.0 41.0 25.0 41.0 58-59 34.64911231605595 38.0 34.0 40.0 25.0 41.0 60-61 34.42443707082336 38.0 34.0 40.0 24.0 41.0 62-63 34.212456749033024 37.5 34.0 40.0 24.0 41.0 64-65 33.96223990601639 37.0 34.0 40.0 23.0 41.0 66-67 33.6839507150722 37.0 33.0 40.0 23.0 41.0 68-69 33.38133432566135 36.0 33.0 39.0 22.5 41.0 70-71 33.05678110854228 36.0 33.0 39.0 22.0 41.0 72-73 32.78569949714379 35.0 33.0 39.0 22.0 40.0 74-75 32.600890036671174 35.0 33.0 37.5 22.0 40.0 76-77 32.297280796413816 35.0 33.0 37.0 22.0 39.0 78-79 31.970350540656693 35.0 32.0 37.0 21.0 39.0 80-81 31.697233820295565 35.0 32.0 36.0 20.5 39.0 82-83 31.43460045987973 35.0 32.0 36.0 20.0 37.0 84-85 31.184546347155866 35.0 32.0 35.5 20.0 37.0 86-87 30.988721026165464 35.0 32.0 35.0 20.0 36.5 88-89 30.834857377681715 35.0 32.0 35.0 19.0 36.0 90-91 30.691624918830914 35.0 32.0 35.0 19.0 36.0 92-93 30.56256568029889 35.0 32.0 35.0 18.0 36.0 94-95 30.453314443548667 35.0 32.0 35.0 18.0 35.5 96-97 30.393682221852757 34.5 32.0 35.0 18.0 35.0 98-99 30.30922245505507 34.0 31.0 35.0 17.0 35.0 100-101 30.2334301349838 34.0 31.0 35.0 15.5 35.0 102-103 29.221285357567467 33.5 29.5 34.5 10.0 35.0 104-105 29.96750178023019 34.0 31.0 35.0 10.5 35.0 106-107 30.118124939221218 34.0 31.0 35.0 10.0 35.0 108-109 30.090504314901544 34.0 31.0 35.0 9.0 35.0 110-111 30.056148224945808 34.0 31.0 35.0 7.0 35.0 112-113 29.975105010053987 34.0 31.0 35.0 4.5 35.0 114-115 29.960269104928493 34.0 31.0 35.0 2.0 35.0 116-117 29.864128832200365 34.0 31.0 35.0 2.0 35.0 118-119 29.73419555554161 34.0 31.0 35.0 2.0 35.0 120-121 29.54577936438724 34.0 31.0 35.0 2.0 35.0 122-123 29.319928147713618 34.0 31.0 35.0 2.0 35.0 124-125 27.259648141816115 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21520420068498325 1101 2 0.6345213590216972 1101 3 0.40138858807563693 1101 4 0.34924247350832616 1101 5 0.5783871280523556 1101 6 0.29200698658873847 1101 7 0.4483998797704132 1101 8 0.533855985620832 1101 9 0.31605831514397664 1101 10-11 0.28635359710004593 1101 12-13 0.4288206240122534 1101 14-15 0.46179519538793556 1101 16-17 0.5318786238676978 1101 18-19 0.41900549027484857 1101 20-21 0.4296425922679532 1101 22-23 0.2229702383993768 1101 24-25 0.29605820640332325 1101 26-27 0.39840447908598264 1101 28-29 0.414202590410099 1101 30-31 0.2913486054848633 1101 32-33 0.19027827332373448 1101 34-35 0.18821396666778867 1101 36-37 0.2918102206803681 1101 38-39 0.23093431666192998 1101 40-41 0.06001227045675961 1101 42-43 0.10671225865518608 1101 44-45 0.2290731446155192 1101 46-47 -0.0354113962151672 1101 48-49 -0.002187333549379389 1101 50-51 0.1904220128246763 1101 52-53 0.11254612082914406 1101 54-55 0.035548980967895716 1101 56-57 -0.23738111133035744 1101 58-59 -0.03983500242588889 1101 60-61 0.1544578756069015 1101 62-63 -0.036293848155949604 1101 64-65 -0.045035040436786744 1101 66-67 -0.12257012379284049 1101 68-69 -0.22031149828747232 1101 70-71 -0.3062622887115367 1101 72-73 -0.1656412677738004 1101 74-75 -0.144834450101456 1101 76-77 -0.10814440364185884 1101 78-79 -0.04861174044160066 1101 80-81 -0.10997124551297688 1101 82-83 -0.07809838599355601 1101 84-85 -0.06217969179360949 1101 86-87 -0.15142629378781436 1101 88-89 -0.012520635727593543 1101 90-91 -0.02196330893752929 1101 92-93 -0.012740110841910735 1101 94-95 0.042127950254521096 1101 96-97 0.04057433349997552 1101 98-99 -0.01992304503805542 1101 100-101 -0.025754935683899305 1101 102-103 -0.028299925101201495 1101 104-105 -0.06429048899726553 1101 106-107 -0.14864796197660013 1101 108-109 -0.19195459422241257 1101 110-111 -0.18337774298124643 1101 112-113 -0.10187170323673911 1101 114-115 -0.17951135884401737 1101 116-117 -0.21950067157357012 1101 118-119 -0.18849811596566468 1101 120-121 -0.04797932708184405 1101 122-123 -0.08840703552359486 1101 124-125 0.01347027524304778 1102 1 0.33446373251696926 1102 2 0.7146573822120814 1102 3 0.42209451982641255 1102 4 0.2962308033368757 1102 5 0.5421460375597889 1102 6 0.4282574206009926 1102 7 0.5267343339416044 1102 8 0.4450138784148976 1102 9 0.36786778578167656 1102 10-11 0.4936125890842291 1102 12-13 0.4935521823844482 1102 14-15 0.5220317133234573 1102 16-17 0.5757943199426165 1102 18-19 0.529119677313652 1102 20-21 0.5773268412854407 1102 22-23 0.45447139595765407 1102 24-25 0.4116413516166162 1102 26-27 0.38608106872437276 1102 28-29 0.43709755886707313 1102 30-31 0.49201245679139305 1102 32-33 0.5617970336116898 1102 34-35 0.5775247494355753 1102 36-37 0.649111719911545 1102 38-39 0.5364204622985582 1102 40-41 0.42834431376915916 1102 42-43 0.4239723926251102 1102 44-45 0.44389900332534893 1102 46-47 0.42537142924211935 1102 48-49 0.4393950203485417 1102 50-51 0.3209148777208455 1102 52-53 0.3116616009649391 1102 54-55 0.3310842781321952 1102 56-57 0.4284206837789455 1102 58-59 0.34375385502315225 1102 60-61 0.5006528137262691 1102 62-63 0.5431876672475866 1102 64-65 0.360430572526937 1102 66-67 0.2876466112583884 1102 68-69 0.23577626620046033 1102 70-71 0.36663354488614885 1102 72-73 0.3643177476268562 1102 74-75 0.4586490678144557 1102 76-77 0.4678775870815315 1102 78-79 0.43834782057447086 1102 80-81 0.40217625290381775 1102 82-83 0.3970487744415969 1102 84-85 0.3276401397692581 1102 86-87 0.37658846205217245 1102 88-89 0.3784605607274081 1102 90-91 0.30740487303270925 1102 92-93 0.3741895739815604 1102 94-95 0.23294776912866055 1102 96-97 0.2648181140033934 1102 98-99 0.3381263971768895 1102 100-101 0.3171138247770955 1102 102-103 0.38094254615593925 1102 104-105 0.4250269841920229 1102 106-107 0.32551293804658954 1102 108-109 0.33270007044328054 1102 110-111 0.38275592010527504 1102 112-113 0.2919829139038903 1102 114-115 0.3070397404037699 1102 116-117 0.36037973137436907 1102 118-119 0.34819353980984147 1102 120-121 0.38072027414596477 1102 122-123 0.20087087189711994 1102 124-125 0.26503681436875226 1103 1 0.16538118302939608 1103 2 0.5815299546300707 1103 3 0.3267847252641971 1103 4 0.2535170591279723 1103 5 0.4357933771638898 1103 6 0.19327216158092142 1103 7 0.41890874547232215 1103 8 0.43658227188207377 1103 9 0.27155069092464146 1103 10-11 0.48692825664003436 1103 12-13 0.432039594055567 1103 14-15 0.46350768318634294 1103 16-17 0.6285208162690736 1103 18-19 0.5788096487676313 1103 20-21 0.44900427120728637 1103 22-23 0.36808047920909104 1103 24-25 0.36050477526769953 1103 26-27 0.41835562532504866 1103 28-29 0.38306601784970695 1103 30-31 0.2357522199997817 1103 32-33 0.2579795877789408 1103 34-35 0.25913069730398064 1103 36-37 0.14224663497542167 1103 38-39 0.09961246941166735 1103 40-41 0.22614135621383014 1103 42-43 0.3590003536901989 1103 44-45 0.2528430343984027 1103 46-47 0.27504560678903545 1103 48-49 0.42090211005793066 1103 50-51 0.3187856250651251 1103 52-53 0.32375914881462364 1103 54-55 0.28876830639744355 1103 56-57 0.20377107453190746 1103 58-59 0.1756827005470356 1103 60-61 0.037752116454520035 1103 62-63 0.09949236096279179 1103 64-65 0.30901731032469115 1103 66-67 0.20795933061820904 1103 68-69 0.09854008294433925 1103 70-71 0.05133977821480329 1103 72-73 0.09446129669326098 1103 74-75 0.20472925406556897 1103 76-77 0.25991336320581837 1103 78-79 0.16294127370751 1103 80-81 0.27549215444343034 1103 82-83 0.15308438677722336 1103 84-85 0.264366238323241 1103 86-87 0.2955470001213385 1103 88-89 0.3207455296075601 1103 90-91 0.19053171565519733 1103 92-93 0.2522313634897273 1103 94-95 0.29715702084844153 1103 96-97 0.1547913228573421 1103 98-99 0.19585195756810947 1103 100-101 0.22561211669529513 1103 102-103 0.27962755715317655 1103 104-105 0.19628675301602172 1103 106-107 0.1256328547483534 1103 108-109 0.1323309609856551 1103 110-111 0.2759557656759597 1103 112-113 0.22457744336245256 1103 114-115 0.1795589270760516 1103 116-117 0.12073059009494358 1103 118-119 0.1143261384340164 1103 120-121 0.06396176883453109 1103 122-123 0.06779433579341543 1103 124-125 0.12512327138592738 1104 1 0.14754851598199537 1104 2 0.40410752934927885 1104 3 0.1909813747977971 1104 4 0.14874835553003152 1104 5 0.284479842041101 1104 6 0.15418021412232719 1104 7 0.325995172276059 1104 8 0.25973570801380674 1104 9 0.3579203844331147 1104 10-11 0.20001363642301584 1104 12-13 0.307479818689103 1104 14-15 0.23512866167482116 1104 16-17 0.3449661808226594 1104 18-19 0.2139419541169545 1104 20-21 0.21031685449302984 1104 22-23 0.2295524951999539 1104 24-25 0.2680742593720211 1104 26-27 0.14046679989196775 1104 28-29 0.1869399946248791 1104 30-31 0.12383522019015203 1104 32-33 0.15815567763235805 1104 34-35 0.2276938992837998 1104 36-37 0.3669610838617743 1104 38-39 0.3640851516884567 1104 40-41 0.20281658941389935 1104 42-43 0.22053899827424317 1104 44-45 0.30002083755849895 1104 46-47 0.24663565057353765 1104 48-49 0.2777410592298395 1104 50-51 0.1572826743702862 1104 52-53 0.022528899433318372 1104 54-55 0.023239085000142268 1104 56-57 0.05942260698954982 1104 58-59 0.10425446906026536 1104 60-61 0.14261124457178198 1104 62-63 0.12635320238673842 1104 64-65 0.13440756351334215 1104 66-67 0.28109610960164844 1104 68-69 0.29201048081222325 1104 70-71 0.2872692470897249 1104 72-73 0.16936849258132725 1104 74-75 0.057458646793527635 1104 76-77 0.1883661520883635 1104 78-79 0.19082771954798972 1104 80-81 0.16861275219709526 1104 82-83 0.21323096968223254 1104 84-85 0.20358668378297295 1104 86-87 0.28427724950264377 1104 88-89 0.24228798153978914 1104 90-91 0.2033029892131637 1104 92-93 0.29501643868485417 1104 94-95 0.22833206368110837 1104 96-97 0.246826826379138 1104 98-99 0.17807878033605107 1104 100-101 0.18701036531513182 1104 102-103 0.27684819298032437 1104 104-105 0.2585702100881697 1104 106-107 0.17984711326729297 1104 108-109 0.21269694232965008 1104 110-111 0.11651116259201189 1104 112-113 0.1722763773049607 1104 114-115 0.08941282008957074 1104 116-117 0.08912246140657487 1104 118-119 0.140332706109632 1104 120-121 0.17068887715359082 1104 122-123 0.24900269292267652 1104 124-125 0.3059033018447117 1105 1 0.15493351559032575 1105 2 0.5418564107939297 1105 3 0.2621530586440599 1105 4 0.1827962126498477 1105 5 0.4560996392035861 1105 6 0.3040346060936088 1105 7 0.4324129843771871 1105 8 0.30610380142412197 1105 9 0.2854743999431335 1105 10-11 0.18891079384096088 1105 12-13 0.2935075533322973 1105 14-15 0.27833035644168547 1105 16-17 0.4017834934329585 1105 18-19 0.34967962466496516 1105 20-21 0.31754633919727837 1105 22-23 0.3850089948013675 1105 24-25 0.16016638409727335 1105 26-27 0.24494044529955517 1105 28-29 0.3530633190957886 1105 30-31 0.2341555348140787 1105 32-33 0.1841377254526222 1105 34-35 0.191062190831623 1105 36-37 0.28507027605976276 1105 38-39 0.39163951278715814 1105 40-41 0.47240893301199094 1105 42-43 0.3389615242049189 1105 44-45 0.2888060242454955 1105 46-47 0.23400046780320594 1105 48-49 0.1763817437718842 1105 50-51 0.2962147362444867 1105 52-53 0.2542456457389761 1105 54-55 0.3114345822100901 1105 56-57 0.36828280945843517 1105 58-59 0.4074927501482719 1105 60-61 0.3457173955894248 1105 62-63 0.3063464979664232 1105 64-65 0.2620752776976829 1105 66-67 0.3509312868425596 1105 68-69 0.3984163125059368 1105 70-71 0.3569160785023584 1105 72-73 0.2630586193432549 1105 74-75 0.3962980909026257 1105 76-77 0.43355014349933185 1105 78-79 0.42893290291733166 1105 80-81 0.3797162859565013 1105 82-83 0.34228541602613305 1105 84-85 0.41212859908149824 1105 86-87 0.4722686190014507 1105 88-89 0.4828430430315329 1105 90-91 0.40279301552398294 1105 92-93 0.4153470748905619 1105 94-95 0.397211112252446 1105 96-97 0.38875525726831484 1105 98-99 0.3654282764677639 1105 100-101 0.4265064279081514 1105 102-103 0.2919292220051659 1105 104-105 0.3509109282675169 1105 106-107 0.3279857726597015 1105 108-109 0.31534265403496775 1105 110-111 0.3741393414318459 1105 112-113 0.391862845895556 1105 114-115 0.40260198150712867 1105 116-117 0.4001787647136261 1105 118-119 0.3885531548219703 1105 120-121 0.321353213784608 1105 122-123 0.2861943702257932 1105 124-125 0.42012231751600737 1106 1 0.13672785729232118 1106 2 0.5055673662048683 1106 3 0.303688537636603 1106 4 0.19066988636055981 1106 5 0.3594580972892274 1106 6 0.23423498738920756 1106 7 0.283072207467292 1106 8 0.20564769466135147 1106 9 0.2245298486894427 1106 10-11 0.11880227743478855 1106 12-13 0.17865391775114148 1106 14-15 0.15917306798432662 1106 16-17 0.06410038708044397 1106 18-19 0.1267954532589144 1106 20-21 0.15742068862171266 1106 22-23 0.06374170980302551 1106 24-25 -0.0328407832947093 1106 26-27 0.2281915936106742 1106 28-29 0.17809046830785036 1106 30-31 0.13862811612094106 1106 32-33 -0.02969429533361989 1106 34-35 0.02592258354999899 1106 36-37 0.2162247007780138 1106 38-39 0.09362804174229211 1106 40-41 0.08942887461101634 1106 42-43 0.05268655698507274 1106 44-45 0.10707201412588319 1106 46-47 0.1878637001280623 1106 48-49 0.017565690744447693 1106 50-51 -0.08084880497806779 1106 52-53 -0.116576392181436 1106 54-55 -0.08223060164034734 1106 56-57 -0.03137289831146717 1106 58-59 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-0.540364112414423 2216 72-73 -0.671754331569872 2216 74-75 -0.5352478314431934 2216 76-77 -0.4718425442853835 2216 78-79 -0.3976936607396411 2216 80-81 -0.250821319664162 2216 82-83 -0.3304993623042627 2216 84-85 -0.3816445131173758 2216 86-87 -0.2926699237290684 2216 88-89 -0.2459670627272068 2216 90-91 -0.2499113945277287 2216 92-93 -0.32757584538988027 2216 94-95 -0.34616925443484803 2216 96-97 -0.4053447138058459 2216 98-99 -0.5165407213969679 2216 100-101 -0.6500888540283007 2216 102-103 -0.5794280177395024 2216 104-105 -0.4645033618464893 2216 106-107 -0.43088037184406147 2216 108-109 -0.46801372323912105 2216 110-111 -0.37030975236557495 2216 112-113 -0.35395109823170046 2216 114-115 -0.4094677388171277 2216 116-117 -0.3477436141824306 2216 118-119 -0.6222012042584808 2216 120-121 -0.487919249845131 2216 122-123 -0.4890137819309892 2216 124-125 -0.8557827695314266 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59.0 3 46.0 4 106.0 5 285.0 6 637.0 7 1424.0 8 2398.0 9 4139.0 10 7254.0 11 9261.0 12 9819.0 13 12128.0 14 16886.0 15 12622.0 16 5824.0 17 4885.0 18 4898.0 19 4423.0 20 4136.0 21 4337.0 22 4835.0 23 5518.0 24 7255.0 25 9260.0 26 12140.0 27 15483.0 28 19613.0 29 25513.0 30 32253.0 31 40097.0 32 50522.0 33 64660.0 34 87387.0 35 122106.0 36 202714.0 37 328950.0 38 140612.0 39 631.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.12110118381969 18.357092127618664 10.790848057507661 21.73095863105398 2 19.067703231645293 21.574783018549788 36.766425458529426 22.59108829127549 3 17.63732497643348 28.518156023696704 31.988277126594173 21.856241873275646 4 11.43183163871494 16.504942691443517 37.161903044039164 34.90132262580238 5 10.183052624392525 42.48277334103409 35.05677087543052 12.277403159142862 6 29.760429639342618 37.44929873046844 18.677908519695464 14.112363110493476 7 26.533272266993745 34.91337258727833 22.448467433551144 16.104887712176776 8 27.949143450478232 32.810269810746625 20.369519322163633 18.87106741661151 9 27.821625640333895 11.22407686830061 19.851370385125747 41.10292710623975 10-11 24.25626374384762 25.91360315453653 29.779094607863126 20.051038493752728 12-13 25.435960336157653 25.465212576738118 29.7274522474818 19.371374839622433 14-15 22.673035237578386 26.399558942088408 27.943183208429666 22.984222611903544 16-17 21.054358975967677 28.688840858400333 28.503093051926253 21.753707113705733 18-19 20.31152459854633 26.45896530198037 32.07880694776005 21.15070315171326 20-21 21.983254856813026 25.562733116045912 32.25510463361765 20.198907393523413 22-23 21.375741501165386 26.1748342895862 31.899725201471867 20.54969900777655 24-25 20.860079286966275 26.082784690008353 32.263050783654805 20.794085239370567 26-27 20.904882379328626 26.198361560830545 32.1114314305522 20.785324629288628 28-29 20.72850626923354 26.120564717249255 32.1648775483956 20.986051465121605 30-31 20.882178562577835 25.914387396911344 32.45865474199994 20.744779298510878 32-33 20.768620266705145 26.202792530248225 31.985246832445046 21.043340370601577 34-35 21.162328814124244 26.213412542030607 31.740101363605174 20.88415728023998 36-37 21.340764291248796 26.086214901232513 31.294094027523773 21.27892677999492 38-39 21.112196851110017 26.21118392365871 31.374165173992015 21.30245405123926 40-41 21.201639693957254 26.330467188867523 31.302642269409215 21.165250847766007 42-43 21.233781027748268 26.322721913027998 31.017964635395895 21.425532423827836 44-45 21.29631880909337 26.45367236734262 30.735179353644526 21.514829469919487 46-47 21.65263114137566 26.72557744088334 29.778843416244506 21.842948001496495 48-49 21.725139009313303 27.064571144650863 29.218485111229022 21.99180473480682 50-51 21.962805201812998 27.117481570833956 28.992868330383047 21.926844896970003 52-53 21.781598152207586 27.151999749026494 29.00418419886513 22.062217899900787 54-55 21.582713218178053 27.42331172864681 29.054329616825303 21.939645436349835 56-57 21.913411408549845 27.316012477917578 28.809088253228786 21.96148786030379 58-59 21.914448752697886 26.969614264919944 28.966481892787233 22.14945508959494 60-61 21.95704309963516 27.17317652373319 29.136564263598235 21.733216113033414 62-63 21.702405152239518 27.132270825098658 29.331124872166487 21.834199150495333 64-65 21.626896286969437 27.08423441316518 29.400030821341698 21.88883847852368 66-67 21.720018241999814 27.328884198985236 28.984780948952594 21.966316610062353 68-69 21.880052309725333 27.141532314195583 29.008762075057003 21.96965330102208 70-71 21.7200796341878 27.036293002677088 29.113848106060754 22.129779257074357 72-73 21.84321045263649 27.355548845763224 28.636247779559792 22.16499292204049 74-75 21.58598703636171 27.56612200563875 28.67222699307116 22.175663964928376 76-77 21.3768475388565 27.455153816322298 28.826958647048468 22.34103999777274 78-79 21.665331493500002 27.348659788074098 28.54890400124538 22.437104717180524 80-81 21.532060896587556 27.575998196237588 28.33808781578096 22.553853091393897 82-83 21.79139850468448 27.379990283233163 28.314376227094723 22.514234984987635 84-85 21.51002362536639 27.159407503259516 28.474036604613318 22.856532266760777 86-87 21.319740823173458 27.26764038352649 28.438045539457647 22.9745732538424 88-89 21.673779856575504 27.134035398119043 28.411272563007024 22.78091218229843 90-91 21.642373676910754 27.401363115724454 28.302199015209474 22.65406419215532 92-93 21.466475676832967 27.33757008700808 28.277331041246196 22.918623194912758 94-95 21.40541078918877 27.16013365221016 28.340236710753388 23.09421884784768 96-97 21.65379834443708 27.24745999317698 28.17456738517522 22.924174277210717 98-99 21.50453769755375 27.216695840940602 28.00765589414444 23.2711105673612 100-101 21.333920997125336 27.135166462696613 28.206136668691332 23.32477587148671 102-103 21.511234186087638 26.957733234988375 27.973493916376203 23.557538662547785 104-105 21.251824356701047 26.903350071797895 28.136893609712754 23.707931961788304 106-107 21.286908580436197 26.994220106500617 28.223917936491755 23.494953376571434 108-109 21.50173338462317 26.99084350993427 27.663767693129948 23.84365541231262 110-111 21.313972798265237 27.100056662111477 27.758474318731242 23.827496220892048 112-113 21.24690874836957 26.95865546534857 27.886913328873092 23.90752245740877 114-115 20.894383238746673 27.247273197918176 27.725684298875546 24.1326592644596 116-117 20.931343480266193 27.196972549314907 27.811585199408878 24.06009877101002 118-119 20.82674166979746 27.086384061102663 27.67694743950637 24.409926829593502 120-121 20.811776862339883 27.29583523686465 27.560336224086647 24.332051676708815 122-123 20.852146483362468 27.108151201649516 27.523314973110622 24.516387341877387 124-125 20.753280854304425 27.170994997055015 27.224443923331577 24.85128022530899 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9.0 1 15.5 2 90.0 3 188.5 4 198.0 5 234.0 6 353.5 7 703.0 8 1126.5 9 1430.5 10 1521.5 11 1463.5 12 1604.5 13 1793.0 14 1972.5 15 2244.5 16 2452.5 17 2682.5 18 2965.5 19 3231.0 20 3562.5 21 3971.0 22 4545.5 23 5492.0 24 6740.5 25 7969.0 26 9844.0 27 11927.5 28 13086.0 29 14841.0 30 17951.5 31 21252.0 32 24351.0 33 27572.0 34 30656.5 35 32332.0 36 35384.0 37 39327.0 38 39858.5 39 39828.5 40 39847.0 41 40064.0 42 40278.5 43 40733.5 44 43057.0 45 44878.5 46 45692.0 47 45295.5 48 45063.5 49 44284.5 50 43750.5 51 42112.5 52 39402.0 53 38656.5 54 37261.5 55 34132.0 56 30392.0 57 27421.5 58 25004.5 59 22417.5 60 19185.0 61 16073.0 62 13277.5 63 10310.0 64 7778.5 65 5922.0 66 4244.5 67 3151.0 68 2366.0 69 1478.5 70 1044.0 71 872.5 72 767.0 73 709.0 74 538.5 75 368.5 76 232.0 77 131.0 78 74.0 79 33.0 80 24.5 81 10.5 82 7.0 83 4.0 84 1.0 85 0.0 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06673902609644927 2 5.489696623679728E-4 3 1.5684847496227794E-4 4 7.842423748113897E-5 5 0.061406177947731816 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.842423748113897E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 2.744848311839864E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.002156666530731322 44-45 0.009489332735217815 46-47 0.01074412053491604 48-49 0.008744302479146995 50-51 0.007763999510632759 52-53 0.006352363235972256 54-55 0.006587635948415674 56-57 0.004979939080052324 58-59 0.003450666449170115 60-61 0.0018821816995473353 62-63 0.0015684847496227796 64-65 0.0019998180557690435 66-67 0.0028624846680615724 68-69 0.001450848393401071 70-71 0.0027840604305804334 72-73 0.002431151361915308 74-75 8.626666122925287E-4 76-77 5.881817811085423E-4 78-79 3.9212118740569484E-5 80-81 2.744848311839864E-4 82-83 3.9212118740569484E-5 84-85 2.744848311839864E-4 86-87 1.5684847496227794E-4 88-89 0.0015684847496227796 90-91 0.0017645453433256268 92-93 3.529090686651254E-4 94-95 0.001215575680957654 96-97 0.0016469089871039184 98-99 0.0015684847496227796 100-101 8.234544935519592E-4 102-103 9.80302968514237E-4 104-105 7.058181373302508E-4 106-107 4.7054542488683383E-4 108-109 7.450302560708202E-4 110-111 0.0010587272059953761 112-113 0.01152836290972743 114-115 0.02125296835738866 116-117 0.019449210895322463 118-119 0.01152836290972743 120-121 0.005764181454863715 122-123 0.0060386662860477 124-125 0.0052544239112363115 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1275116.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.724129166566026 #Duplication Level Percentage of deduplicated Percentage of total 1 42.27742907113344 12.566597625407038 2 18.538441185118334 11.020780406664892 3 12.6116121993076 11.246075700325768 4 8.651741000071734 10.286618680072293 5 5.606585989632086 8.332544306964177 6 3.5484699078528212 6.328510673081393 7 2.246051463620373 4.673334667358465 8 1.4931428436419776 3.550589659883827 9 0.9769610084280406 2.613538368469231 >10 3.744025964371326 18.816411518551813 >50 0.21236792375318878 4.330982935124585 >100 0.08889997543540265 4.499109126881786 >500 0.0034705674522851563 0.6849003588123517 >1k 5.339334541977163E-4 0.6007062583952615 >5k 2.6696672709885816E-4 0.44929971400712954 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5662 0.4440380326182088 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3828 0.3002079810778 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3742 0.293463496654422 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.5684847496227794E-4 0.0 24-25 0.0 0.0 0.0 3.9212118740569485E-4 0.0 26-27 0.0 0.0 0.0 5.489696623679728E-4 0.0 28-29 0.0 0.0 0.0 9.018787310330983E-4 0.0 30-31 0.0 0.0 0.0 0.003254605855467267 0.0 32-33 0.0 0.0 0.0 0.008979575191590412 0.0 34-35 0.0 0.0 0.0 0.018390483689327088 0.0 36-37 0.0 0.0 0.0 0.034036119066814315 0.0 38-39 0.0 0.0 0.0 0.0579555114985617 0.0 40-41 0.0 0.0 0.0 0.08418841893600268 0.0 42-43 0.0 0.0 0.0 0.11951853792125579 0.0 44-45 0.0 0.0 0.0 0.1610049595487783 0.0 46-47 0.0 0.0 0.0 0.21233362298018377 0.0 48-49 0.0 0.0 0.0 0.2700146496475615 0.0 50-51 0.0 0.0 0.0 0.32483319164687763 0.0 52-53 0.0 0.0 0.0 0.38596488476342544 0.0 54-55 0.0 0.0 0.0 0.463055910207385 0.0 56-57 0.0 0.0 0.0 0.5630468129958373 0.0 58-59 0.0 0.0 0.0 0.6784088663305927 0.0 60-61 0.0 0.0 0.0 0.7878498897355222 0.0 62-63 0.0 0.0 0.0 0.9017610946768764 0.0 64-65 0.0 0.0 0.0 1.0346509650886664 0.0 66-67 0.0 0.0 0.0 1.1915778642884254 0.0 68-69 0.0 0.0 0.0 1.395480881739387 0.0 70-71 0.0 0.0 0.0 1.5844048698314506 0.0 72-73 0.0 0.0 0.0 1.7699174035930847 0.0 74-75 0.0 0.0 0.0 1.977192663255735 0.0 76-77 0.0 0.0 0.0 2.232581192613064 0.0 78-79 0.0 0.0 0.0 2.5316520222473877 0.0 80-81 0.0 0.0 0.0 2.840761154279297 0.0 82-83 0.0 0.0 0.0 3.1575166494656175 0.0 84-85 0.0 0.0 0.0 3.50336753675744 0.0 86-87 0.0 0.0 0.0 3.879568603954464 0.0 88-89 0.0 0.0 0.0 4.317489546049144 0.0 90-91 0.0 0.0 0.0 4.767919154022065 0.0 92-93 0.0 0.0 0.0 5.216858701482845 0.0 94-95 0.0 0.0 0.0 5.695756307661421 0.0 96-97 0.0 0.0 0.0 6.212964153849532 0.0 98-99 0.0 0.0 0.0 6.791264481035451 0.0 100-101 0.0 0.0 0.0 7.425167592595497 0.0 102-103 0.0 0.0 0.0 8.050718522863802 0.0 104-105 0.0 0.0 0.0 8.681014119499716 0.0 106-107 0.0 0.0 0.0 9.378911408844372 0.0 108-109 0.0 0.0 0.0 10.103551363170096 0.0 110-111 0.0 0.0 0.0 10.881990344407882 0.0 112-113 0.0 0.0 0.0 11.661762537682847 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4365 0.0 80.55619 1 GTATCAA 6570 0.0 53.101044 2 GTACATG 11420 0.0 48.42984 1 TACATGG 11625 0.0 47.320553 2 ACATGGG 11680 0.0 46.537037 3 CATGGGA 8725 0.0 42.98787 4 CATGGGG 2885 0.0 39.20835 4 ATCAACG 9130 0.0 39.12472 4 TCAACGC 9180 0.0 38.846775 5 CAACGCA 9325 0.0 38.27953 6 ATGGGAG 2955 0.0 38.078087 5 AACGCAG 9505 0.0 37.867565 7 TATCAAC 9625 0.0 37.421864 3 ATGGGAT 3550 0.0 36.894794 5 ATGGGAC 1605 0.0 35.23866 5 ACGCAGA 11215 0.0 31.828495 8 CGCAGAG 11315 0.0 31.442045 9 TGGGATA 1185 0.0 29.62087 6 TACCTGG 980 0.0 29.159895 2 CGTTTAA 355 0.0 28.539852 1 >>END_MODULE