FastQCFastQC Report
Thu 26 May 2016
SRR936229_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936229_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664338
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23200.061.0310861
GTACATG139300.057.0154151
TACATGG141500.055.710192
ACATGGG143450.053.2109833
CATGGGA92300.049.4389044
GTATCAA30900.046.0166132
ATGGGAG36600.043.401565
CATGGGG49300.042.7200284
ATGGGAT41800.039.2833025
ATGGGGG29150.038.1661385
TCAACGC39100.038.0398185
ATCAACG39500.037.8052254
CAACGCA39150.037.6873056
AACGCAG41900.035.639777
GAGTACA80800.031.2924611
AGTACAT77500.030.8602582
TACCTGG16300.030.2946342
TATCAAC49150.029.6563533
ACGCAGA50750.028.9558358
CGCAGAG52250.027.6691069
Produced by FastQC (version 0.11.5)