##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936229_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1664338 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.093404104214407 31.0 30.0 31.0 25.0 34.0 2 29.06401584293575 31.0 30.0 33.0 23.0 34.0 3 29.173054391595937 31.0 30.0 34.0 23.0 34.0 4 32.8032677256663 35.0 33.0 37.0 26.0 37.0 5 32.86115320325559 35.0 33.0 37.0 26.0 37.0 6 33.03060856628882 35.0 33.0 37.0 26.0 37.0 7 32.79118844850024 35.0 33.0 37.0 26.0 37.0 8 32.77948589769626 35.0 33.0 37.0 26.0 37.0 9 34.226782059894084 38.0 34.0 39.0 25.0 39.0 10-11 33.978216864603226 37.5 33.5 39.0 24.5 39.0 12-13 33.992738253888334 38.0 34.0 39.0 25.0 39.0 14-15 34.97437569772486 39.0 34.0 41.0 25.0 41.0 16-17 34.92494403180123 38.5 34.0 40.5 25.0 41.0 18-19 34.906859664322994 38.5 34.0 40.0 25.0 41.0 20-21 35.01143938310608 39.0 34.0 40.5 25.0 41.0 22-23 34.83741493614879 39.0 34.0 40.0 24.5 41.0 24-25 34.76750395652806 39.0 34.0 40.0 24.5 41.0 26-27 34.85299320210198 39.0 34.0 40.0 25.0 41.0 28-29 34.710722821926794 38.5 34.0 40.0 24.0 41.0 30-31 34.631098971482956 38.0 33.5 40.0 24.0 41.0 32-33 34.475745311349016 38.0 33.0 40.0 23.5 41.0 34-35 34.43299888604358 38.0 33.0 40.0 24.0 41.0 36-37 34.3018848935733 38.0 33.0 40.0 23.5 41.0 38-39 34.04008320425298 38.0 33.0 40.0 22.0 41.0 40-41 34.12761530410289 38.0 33.0 40.0 22.5 41.0 42-43 34.005808916217745 38.0 33.0 40.0 21.5 41.0 44-45 33.81646907058543 38.0 33.0 40.0 20.5 41.0 46-47 33.77715313836492 38.0 33.0 40.0 21.0 41.0 48-49 33.67490918311064 38.0 33.0 40.0 20.0 41.0 50-51 33.51771094573338 38.0 32.0 40.0 19.0 41.0 52-53 33.345492622291864 38.0 32.0 40.0 18.5 41.0 54-55 33.16153449599781 38.0 32.0 40.0 17.5 41.0 56-57 32.9440390113066 37.0 31.5 40.0 15.0 41.0 58-59 32.709124288455826 37.0 31.0 40.0 13.5 41.0 60-61 32.44860929690964 36.5 31.0 40.0 12.5 41.0 62-63 32.19204512544928 36.0 31.0 39.5 9.0 41.0 64-65 31.900695952384673 36.0 30.5 39.0 8.0 41.0 66-67 31.599471982253604 35.0 30.0 39.0 7.5 40.5 68-69 31.322006106932605 35.0 30.0 39.0 7.0 40.0 70-71 30.98383621596094 35.0 30.0 38.0 4.0 40.0 72-73 30.66540029729538 35.0 30.0 37.5 2.0 40.0 74-75 30.321541958424312 34.0 29.0 37.0 2.0 39.0 76-77 30.007554655364473 34.0 29.0 36.5 2.0 39.0 78-79 29.67556890487389 34.0 29.0 36.0 2.0 38.5 80-81 29.36512805692113 34.0 29.0 35.5 2.0 37.0 82-83 29.06634409597089 34.0 29.0 35.0 2.0 37.0 84-85 29.113992470279474 34.0 29.0 35.0 2.0 37.0 86-87 29.00822819643606 34.0 29.0 35.0 2.0 36.0 88-89 28.842437053050524 34.0 29.0 35.0 2.0 36.0 90-91 28.675236039794804 34.0 29.0 35.0 2.0 36.0 92-93 28.551229077266758 34.0 29.0 35.0 2.0 35.0 94-95 28.428275686789583 34.0 29.0 35.0 2.0 35.0 96-97 28.31984158265929 34.0 29.0 35.0 2.0 35.0 98-99 28.202814572520726 34.0 29.0 35.0 2.0 35.0 100-101 28.089349939735797 34.0 28.5 35.0 2.0 35.0 102-103 27.097016050826213 32.5 26.0 34.0 2.0 35.0 104-105 27.770746386851712 33.5 27.0 35.0 2.0 35.0 106-107 27.899861686748725 34.0 28.0 35.0 2.0 35.0 108-109 27.844702217938902 34.0 27.5 35.0 2.0 35.0 110-111 27.718384426721016 34.0 27.0 35.0 2.0 35.0 112-113 27.666014355257165 34.0 27.0 35.0 2.0 35.0 114-115 27.534641100545684 34.0 27.0 35.0 2.0 35.0 116-117 27.381181586913236 34.0 27.0 35.0 2.0 35.0 118-119 27.140580218681542 34.0 27.0 35.0 2.0 35.0 120-121 26.884436634866233 34.0 25.5 35.0 2.0 35.0 122-123 26.524725746813445 34.0 25.0 35.0 2.0 35.0 124-125 24.431532236841313 31.5 13.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6619507947781784 1101 2 0.7261367807085897 1101 3 0.6597505967692889 1101 4 0.8259922408069613 1101 5 0.6911902083575043 1101 6 0.6858561587579857 1101 7 0.7289247084934019 1101 8 0.6767399730248016 1101 9 0.8916767836842254 1101 10-11 0.9245324888925381 1101 12-13 0.8307164129922242 1101 14-15 1.045407696168546 1101 16-17 0.9341858841547364 1101 18-19 0.95491430675321 1101 20-21 0.8974015172204872 1101 22-23 1.0893334916188167 1101 24-25 1.1877979235276612 1101 26-27 1.0508729015341274 1101 28-29 1.178561348995565 1101 30-31 1.1122498632124476 1101 32-33 1.1594795535945224 1101 34-35 1.1454989916801779 1101 36-37 1.0931791115097909 1101 38-39 1.2682950723445856 1101 40-41 1.224357116342503 1101 42-43 1.2544194115177945 1101 44-45 1.1928782073494872 1101 46-47 1.1801940583852115 1101 48-49 1.2862752125413337 1101 50-51 1.2280020368326348 1101 52-53 1.1316066553720106 1101 54-55 1.0232064347959096 1101 56-57 1.0501076199190535 1101 58-59 1.3219838928176202 1101 60-61 1.3097635652965494 1101 62-63 1.1740162963220442 1101 64-65 1.208205893726582 1101 66-67 1.1391850938505002 1101 68-69 1.035054342806859 1101 70-71 1.0519777738023315 1101 72-73 1.1686337774431834 1101 74-75 1.1617558994650068 1101 76-77 1.0480964019056636 1101 78-79 1.2140876004577805 1101 80-81 1.167028994887513 1101 82-83 1.0620004525905955 1101 84-85 0.8429538092482787 1101 86-87 0.9013411050719142 1101 88-89 0.9811402359092121 1101 90-91 0.9597405562608188 1101 92-93 1.0264174342888808 1101 94-95 1.0738849129945436 1101 96-97 0.9417837428999611 1101 98-99 0.9703818520961107 1101 100-101 1.0793414833544794 1101 102-103 1.186362846711404 1101 104-105 1.1303445284190907 1101 106-107 1.0702650030182213 1101 108-109 1.0683791971576362 1101 110-111 1.1582833706882099 1101 112-113 1.1157675054969154 1101 114-115 1.1441853945846425 1101 116-117 1.1052159818764515 1101 118-119 1.1311475456657796 1101 120-121 1.11077986002298 1101 122-123 1.3649871860678147 1101 124-125 1.123495860472854 1102 1 0.40176914331313185 1102 2 0.3478132775421905 1102 3 0.3415077359214642 1102 4 0.40259321852870045 1102 5 0.4564201421645606 1102 6 0.32529850547015826 1102 7 0.5569054120564587 1102 8 0.5236440666852147 1102 9 0.5434314763570782 1102 10-11 0.6528475846299813 1102 12-13 0.5816330927462801 1102 14-15 0.7387428624863901 1102 16-17 0.6405676402289018 1102 18-19 0.6277822210193307 1102 20-21 0.5404814831947746 1102 22-23 0.6994936857651695 1102 24-25 0.8733400556966444 1102 26-27 0.6933577475371777 1102 28-29 0.7431622031183451 1102 30-31 0.5922665820711686 1102 32-33 0.8259626218125931 1102 34-35 0.7968291879400198 1102 36-37 0.639180272799976 1102 38-39 0.6734015991944347 1102 40-41 0.5847411460974499 1102 42-43 0.8365455925515661 1102 44-45 0.7940000028703196 1102 46-47 0.832401072235939 1102 48-49 0.7222931031901609 1102 50-51 0.6309147572201539 1102 52-53 0.7427515161979272 1102 54-55 0.8720250372787106 1102 56-57 0.9272682579116562 1102 58-59 0.8768122545179651 1102 60-61 0.7618967197713928 1102 62-63 0.8410822596146588 1102 64-65 0.7578575741698792 1102 66-67 0.6062331472944535 1102 68-69 0.6554158757518991 1102 70-71 0.6517643556057138 1102 72-73 0.6283903924997887 1102 74-75 0.4991865981756014 1102 76-77 0.6409104426270176 1102 78-79 0.6147930894963949 1102 80-81 0.7203014197355238 1102 82-83 0.7437882664835662 1102 84-85 0.7167932503050025 1102 86-87 0.7656435517162379 1102 88-89 0.7409626404098937 1102 90-91 0.6537471919047455 1102 92-93 0.7263921445025758 1102 94-95 0.7154842414802189 1102 96-97 0.7348808162296905 1102 98-99 0.7469417901646338 1102 100-101 0.714096369675687 1102 102-103 0.6389177976220708 1102 104-105 0.6438159145858755 1102 106-107 0.5334272642971172 1102 108-109 0.6723303437704509 1102 110-111 0.7271402888472522 1102 112-113 0.6461848484703694 1102 114-115 0.6662018335457347 1102 116-117 0.670860902176873 1102 118-119 0.6328939809084027 1102 120-121 0.5933323074547978 1102 122-123 0.5022455512346298 1102 124-125 0.593690212330575 1103 1 0.3973223759441069 1103 2 0.4512709451486785 1103 3 0.5135707548246593 1103 4 0.48176100263903265 1103 5 0.44245312746171095 1103 6 0.5545727517118593 1103 7 0.38796835329142 1103 8 0.5278843647523459 1103 9 0.5371439270820986 1103 10-11 0.8070834327991605 1103 12-13 0.5810328410177092 1103 14-15 0.6333320847291972 1103 16-17 0.579868646979179 1103 18-19 0.6440729470891284 1103 20-21 0.3957412103959115 1103 22-23 0.5429357660574325 1103 24-25 0.6648760516441854 1103 26-27 0.5687539710720273 1103 28-29 0.5872268218977581 1103 30-31 0.694441009424466 1103 32-33 0.7339149546846357 1103 34-35 0.728159287034849 1103 36-37 0.634413440744197 1103 38-39 0.7214926227887375 1103 40-41 0.5919179295859962 1103 42-43 0.8570583549632431 1103 44-45 0.6478751931463123 1103 46-47 0.5600438383044377 1103 48-49 0.56853861919123 1103 50-51 0.5079782390021848 1103 52-53 0.5173630669404119 1103 54-55 0.5851893112618427 1103 56-57 0.4883096771443718 1103 58-59 0.5381839650035758 1103 60-61 0.5319084773409486 1103 62-63 0.4948789319258182 1103 64-65 0.4714964293967405 1103 66-67 0.5673850909606841 1103 68-69 0.5432402095827982 1103 70-71 0.5818379734651735 1103 72-73 0.5270198537460935 1103 74-75 0.5687320183659743 1103 76-77 0.5350435689643156 1103 78-79 0.526412749488415 1103 80-81 0.4409924314932425 1103 82-83 0.4415484397925553 1103 84-85 0.5185841367052966 1103 86-87 0.4384257844439645 1103 88-89 0.5154561235082582 1103 90-91 0.517320774507894 1103 92-93 0.534852287504556 1103 94-95 0.556669470681264 1103 96-97 0.6330617844112076 1103 98-99 0.4688775807460246 1103 100-101 0.537818246432451 1103 102-103 0.4634484079259451 1103 104-105 0.46849105340107755 1103 106-107 0.5097131096413179 1103 108-109 0.5645764748562421 1103 110-111 0.5666105557024892 1103 112-113 0.6613399188421099 1103 114-115 0.5544988106413591 1103 116-117 0.5758060879314151 1103 118-119 0.44470645490408245 1103 120-121 0.41445706872932675 1103 122-123 0.46690687673176967 1103 124-125 0.4368575468297813 1104 1 0.7203995755031336 1104 2 0.6811781069914673 1104 3 0.6431272988364647 1104 4 0.7094136455745002 1104 5 0.5635685935939918 1104 6 0.6662121677435877 1104 7 0.7217410023312496 1104 8 0.7102536586320198 1104 9 0.8703929031578284 1104 10-11 0.9360836755595088 1104 12-13 0.8797473159571254 1104 14-15 0.9745914894831813 1104 16-17 0.9469217072031881 1104 18-19 0.9008387870546741 1104 20-21 0.9741832221957054 1104 22-23 0.8517989641186432 1104 24-25 1.0152016274487465 1104 26-27 0.8204272203545031 1104 28-29 0.9227594098232572 1104 30-31 0.8648778513715669 1104 32-33 0.9501397425197524 1104 34-35 0.9009710705751104 1104 36-37 0.9333129320669826 1104 38-39 0.9316146687871054 1104 40-41 0.8392337926196589 1104 42-43 0.9907206128584036 1104 44-45 0.9469239054853134 1104 46-47 0.8586889321000797 1104 48-49 0.7830204083350125 1104 50-51 0.7339143246800859 1104 52-53 0.7883674348590404 1104 54-55 0.8303037592147859 1104 56-57 0.8367468631983783 1104 58-59 1.0085558597186477 1104 60-61 0.9741558931511349 1104 62-63 0.8849235921703311 1104 64-65 0.936693904924784 1104 66-67 1.000387285231426 1104 68-69 0.9552418658918853 1104 70-71 0.9039253501037336 1104 72-73 1.027300913666064 1104 74-75 0.9461451858759169 1104 76-77 0.9961174329254234 1104 78-79 0.9874595091385814 1104 80-81 0.9918973472135661 1104 82-83 1.0381420907844898 1104 84-85 1.016286825394019 1104 86-87 0.9505038483948169 1104 88-89 1.0015370286979675 1104 90-91 1.169789815197948 1104 92-93 0.9868104119333765 1104 94-95 1.0629606905932718 1104 96-97 1.088793152996253 1104 98-99 1.0856699829362881 1104 100-101 1.1366216352962297 1104 102-103 1.1412277929762666 1104 104-105 1.060222058434821 1104 106-107 1.2422152146446024 1104 108-109 1.2172805423294797 1104 110-111 1.30570460589378 1104 112-113 1.2597744533874398 1104 114-115 1.1481271174409464 1104 116-117 1.1844738992642014 1104 118-119 1.1798230947708674 1104 120-121 1.123592237927987 1104 122-123 1.2039704604991144 1104 124-125 1.0790706992555847 1105 1 0.3564393253029756 1105 2 0.3262152235274236 1105 3 0.3278543683081274 1105 4 0.36715749165168887 1105 5 0.3149150058149033 1105 6 0.22522350830283955 1105 7 0.2011281814630621 1105 8 0.26794951264450617 1105 9 0.30149794621896575 1105 10-11 0.3850607516171962 1105 12-13 0.4574831499939265 1105 14-15 0.37659246650540723 1105 16-17 0.34011530994114736 1105 18-19 0.33053885955820306 1105 20-21 0.22821028903295826 1105 22-23 0.26006367872621183 1105 24-25 0.47604285160147697 1105 26-27 0.3761237868028928 1105 28-29 0.48505588738896677 1105 30-31 0.46125757168893244 1105 32-33 0.6064607212371627 1105 34-35 0.6031327751982687 1105 36-37 0.4895256422899834 1105 38-39 0.7101845510748319 1105 40-41 0.7087891558760688 1105 42-43 0.8328490552009171 1105 44-45 0.688282613525196 1105 46-47 0.5904383459440226 1105 48-49 0.5967729697253361 1105 50-51 0.6072066834041578 1105 52-53 0.5189094588413283 1105 54-55 0.531737851448888 1105 56-57 0.5322324879141789 1105 58-59 0.5476585111943209 1105 60-61 0.5467743871624293 1105 62-63 0.5703420509034558 1105 64-65 0.7017100378181347 1105 66-67 0.6851754901793043 1105 68-69 0.6634325020559118 1105 70-71 0.6186430216066405 1105 72-73 0.5419516654462981 1105 74-75 0.6133853615337479 1105 76-77 0.5354137993877899 1105 78-79 0.5281117373334041 1105 80-81 0.603145730774397 1105 82-83 0.5181175735972587 1105 84-85 0.5164685137296559 1105 86-87 0.5149200232096334 1105 88-89 0.49689416869162173 1105 90-91 0.44514958387390635 1105 92-93 0.4781264539968788 1105 94-95 0.48430453140164786 1105 96-97 0.5188213812643809 1105 98-99 0.49610937376923303 1105 100-101 0.5020200725188317 1105 102-103 0.5703318303802973 1105 104-105 0.5740547115942185 1105 106-107 0.5815059400349227 1105 108-109 0.4901051215971357 1105 110-111 0.6329480631092572 1105 112-113 0.5701145957142657 1105 114-115 0.7680591725894423 1105 116-117 0.7748891165285166 1105 118-119 0.8100579809930224 1105 120-121 0.7951336594323237 1105 122-123 0.8751761379947318 1105 124-125 0.8143115644414074 1106 1 -0.07923127684295395 1106 2 -0.1781257524978841 1106 3 0.08189729862480277 1106 4 0.024074121833891127 1106 5 0.08052618880839901 1106 6 -0.0768755381464743 1106 7 -0.2740096236408931 1106 8 -0.09217855131706187 1106 9 -0.13126176845064208 1106 10-11 -0.006815781044622327 1106 12-13 0.02983868758121133 1106 14-15 -0.22146156812684836 1106 16-17 0.0077564168076023066 1106 18-19 -0.20163708505880606 1106 20-21 -0.1117884089815675 1106 22-23 -0.13450015474369081 1106 24-25 -3.9943311115948035 1106 26-27 -1.023616905747211 1106 28-29 -0.596946985360951 1106 30-31 -0.5375028878504722 1106 32-33 -5.699340601926529 1106 34-35 -1.1865563380518367 1106 36-37 -0.4282115768087422 1106 38-39 0.0625582577855539 1106 40-41 -0.6152415005732763 1106 42-43 -0.19925284946971544 1106 44-45 -0.06497821747122856 1106 46-47 -0.06888185838388239 1106 48-49 0.14039465186262845 1106 50-51 0.12970791204817544 1106 52-53 0.4070457169294954 1106 54-55 0.006531720047263434 1106 56-57 0.13631400282176998 1106 58-59 0.36249040279761147 1106 60-61 0.33874504834474806 1106 62-63 0.2844866701970332 1106 64-65 0.3146323414159973 1106 66-67 0.3451252435983392 1106 68-69 0.48548524420215955 1106 70-71 0.40075966441754574 1106 72-73 0.401470646075488 1106 74-75 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68-69 -1.5693070221377 2216 70-71 -1.6278020325254907 2216 72-73 -1.7714803232029652 2216 74-75 -1.6344021721560793 2216 76-77 -1.39478595666138 2216 78-79 -1.2192721661389747 2216 80-81 -1.2218739223354333 2216 82-83 -1.290187049945974 2216 84-85 -1.5353134250453664 2216 86-87 -1.3809207105791899 2216 88-89 -1.2955159809720094 2216 90-91 -1.2289767987470874 2216 92-93 -1.2806908549213745 2216 94-95 -1.4086811322257482 2216 96-97 -1.5869082587615573 2216 98-99 -1.5020049163086213 2216 100-101 -1.5078010279325937 2216 102-103 -1.3114166179956008 2216 104-105 -1.3312796844502053 2216 106-107 -1.6353916094796652 2216 108-109 -1.4196821611874206 2216 110-111 -1.4490626767272055 2216 112-113 -1.5712684786798263 2216 114-115 -1.638129358176112 2216 116-117 -1.5796995181445475 2216 118-119 -1.685525949888028 2216 120-121 -1.5935272973212875 2216 122-123 -1.5390388046413719 2216 124-125 -1.6645941544942353 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57984.0 3 10055.0 4 6678.0 5 5525.0 6 6169.0 7 6912.0 8 7812.0 9 8106.0 10 9261.0 11 10226.0 12 10454.0 13 10592.0 14 10458.0 15 10015.0 16 9312.0 17 8413.0 18 7772.0 19 7450.0 20 7472.0 21 7990.0 22 9462.0 23 12349.0 24 17123.0 25 22675.0 26 26666.0 27 29622.0 28 34868.0 29 41216.0 30 49375.0 31 60286.0 32 75049.0 33 96875.0 34 129524.0 35 179793.0 36 270963.0 37 310850.0 38 78901.0 39 85.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.39388151289435 17.42962496087021 9.165473495989083 23.01102003024635 2 19.169626806125688 20.597403408927043 36.97792993722421 23.255039847723054 3 17.57764057257669 28.235885061670242 29.946597321694657 24.23987704405841 4 10.967282969984355 15.546417421524069 36.92147960863578 36.5648199998558 5 11.085695402039866 42.688116804109775 34.48094572874889 11.745242065101467 6 31.522733786408818 36.473978402106795 19.00309373927871 13.000194072205677 7 29.058429658612557 33.67771698573477 21.2153748724709 16.04847848318178 8 28.500091028112966 32.649170442480745 19.857948076122334 18.992790453283952 9 28.811235366360716 12.617375546470742 18.290276606125282 40.281112481043266 10-11 24.82521327105342 26.155310836777197 29.14350897727232 19.87596691489707 12-13 25.316054116071417 25.23526995998045 28.392851939075108 21.055823984873022 14-15 23.239553193669053 26.46036578129971 25.222728707946672 25.077352317084568 16-17 23.24140398671276 28.938592029638826 25.663948913163974 22.15605507048444 18-19 22.45070918448891 27.95156059042027 27.319074737458138 22.278655487632683 20-21 23.73439844739297 27.30887957231204 26.781329660111847 22.17539232018314 22-23 23.50325780110153 27.43667525069492 26.622194905152703 22.437872043050845 24-25 23.070255947293393 27.451958246898485 26.75328928097722 22.724496524830897 26-27 22.75935931695725 27.657012559202194 26.620671836954184 22.962956286886367 28-29 22.931104512478985 27.669531904359623 26.696340483008658 22.703023100152738 30-31 22.978141863087874 27.276796631423107 26.924220839344287 22.820840666144733 32-33 22.677070543415596 27.571665348306855 26.83902180806464 22.91224230021291 34-35 23.053235964588666 27.674265738803577 26.691465494139972 22.581032802467785 36-37 23.060730389348517 27.262717793901036 26.589324302279927 23.087227514470523 38-39 22.882637564943824 27.357279806864415 26.8651878271225 22.894894801069263 40-41 22.924666666266102 27.377167773322846 26.88976238386419 22.80840317654686 42-43 22.884275386156787 27.24020506904482 26.955043133296382 22.920476411502012 44-45 22.690070812683015 27.249679373654295 27.05485511955179 23.0053946941109 46-47 22.877503884483335 27.50899169267338 26.491184956684855 23.122319466158427 48-49 22.69294423687065 27.3735237718139 26.7465464424369 23.186985548878546 50-51 22.880615313808057 27.192883105818193 26.978170984392136 22.948330595981606 52-53 23.058546433378197 27.005623918477216 26.94121322822534 22.994616419919247 54-55 22.635004410220176 27.252757288880236 27.11624475159765 22.995993549301936 56-57 22.86208609997711 27.541141402409753 26.84401026003998 22.752762237573158 58-59 23.089596248334537 27.023888026845675 26.648661396269663 23.23785432855012 60-61 22.926899171405577 27.180727075759563 27.230206609348116 22.66216714348674 62-63 22.656109411552997 27.24224255950548 27.13258826494761 22.969059763993915 64-65 22.61335533225502 27.29799005482106 26.973233577115995 23.11542103580793 66-67 22.64199010714506 27.648231348002877 26.70521064751184 23.004567897340227 68-69 22.852023692359825 27.195562909848626 26.90634812483552 23.046065272956028 70-71 22.485211011401287 27.1172264867023 27.231957373825317 23.165605128071093 72-73 22.709504222109263 27.294611538309017 26.845723274294702 23.150160965287014 74-75 22.396185858294544 27.661089355642577 27.069470793846783 22.8732539922161 76-77 22.344082997913276 27.29616284260763 27.142527538091883 23.217226621387212 78-79 22.712838889309477 27.335408637929646 26.88141322595104 23.070339246809834 80-81 22.57114797812093 27.361362421706502 26.83565553836813 23.23183406180443 82-83 22.777258551536033 27.37509955174302 26.77080432400799 23.076837572712954 84-85 22.322115516049536 27.2141998153613 27.028599771139344 23.43508489744982 86-87 22.222806373484154 27.198613498620766 27.280538209601087 23.298041918293997 88-89 22.46600059303125 27.19270601602266 27.071335891519144 23.269957499426948 90-91 22.391148651652852 27.341056542737213 27.16071413550405 23.10708067010588 92-93 22.499657520254374 27.06542324488143 27.27746026634109 23.157458968523105 94-95 22.216497539855308 26.98360244013668 27.3869772455392 23.41292277446881 96-97 22.430019971976744 27.044904502226718 27.387324163688493 23.13775136210804 98-99 22.174832462662913 27.11258370858432 27.04096331828324 23.671620510469523 100-101 22.065545895345274 27.12620093370906 27.168710532166095 23.639542638779567 102-103 22.130586835327584 26.883923847829045 27.283334900534783 23.702154416308588 104-105 22.016810963928137 26.7474519033329 27.49520002187066 23.740537110868303 106-107 21.923626924948778 26.9094170026377 27.446179543720294 23.72077652869323 108-109 22.036792026571685 27.083363375488485 27.06395614326022 23.815888454679605 110-111 22.040977573490757 27.12793474832139 27.08854941192375 23.742538266264102 112-113 22.058669784634315 26.95643056793957 27.40712189635241 23.577777751073704 114-115 21.800627940183755 27.114746740506167 27.250927625252313 23.833697694057758 116-117 21.642838709832475 26.98145466048879 27.487635388849483 23.888071240829248 118-119 21.675709891388173 27.129584037699445 27.126609852723764 24.068096218188614 120-121 21.733383243337105 27.0458545155435 27.270209195417493 23.950553045701902 122-123 21.789134903875702 26.859885617601563 27.241030096504325 24.109949382018407 124-125 21.830206302049803 26.624804012902477 27.11587204832691 24.42911763672081 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4.0 1 6.5 2 9.5 3 12.0 4 14.0 5 14.5 6 18.5 7 27.0 8 31.5 9 32.5 10 47.0 11 57.5 12 58.5 13 67.5 14 95.5 15 120.5 16 158.0 17 228.5 18 291.5 19 391.0 20 541.5 21 800.5 22 1219.5 23 1921.5 24 2800.5 25 3946.0 26 5872.5 27 7884.0 28 9682.5 29 12300.0 30 16536.5 31 20734.5 32 25081.0 33 29462.5 34 34430.5 35 38887.5 36 43682.5 37 49041.5 38 50688.5 39 51364.0 40 52583.0 41 54941.5 42 57339.5 43 59793.0 44 63809.5 45 66285.5 46 66636.0 47 66304.0 48 65878.0 49 65142.5 50 63513.5 51 60087.0 52 56671.5 53 55270.0 54 53893.5 55 50730.0 56 46077.5 57 41695.5 58 38444.5 59 34753.0 60 29270.0 61 23681.0 62 19239.0 63 14851.0 64 11231.5 65 9120.0 66 7029.0 67 5220.5 68 3869.5 69 2759.5 70 2131.5 71 1701.0 72 1418.0 73 1195.0 74 980.0 75 711.0 76 463.5 77 335.0 78 228.0 79 149.0 80 86.0 81 56.5 82 42.0 83 30.0 84 26.5 85 19.5 86 16.0 87 12.5 88 7.5 89 8.0 90 7.0 91 5.0 92 4.5 93 2.0 94 2.5 95 4.5 96 2.5 97 3.0 98 3.5 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.004197464697676E-4 2 1.2016789858790702E-4 3 1.2016789858790702E-4 4 3.60503695763721E-4 5 7.810913408213957E-4 6 5.407555436455815E-4 7 7.21007391527442E-4 8 0.0010214271379972097 9 0.0013218468844669773 10-11 0.001442014783054884 12-13 0.0015922246562897681 14-15 0.002253148098523257 16-17 0.0020728962506413964 18-19 0.0019226863774065123 20-21 0.002433399946405117 22-23 0.09373096089856749 24-25 0.001832560453465582 26-27 0.0012317209605260469 28-29 0.0012016789858790703 30-31 9.012592394093027E-4 32-33 0.001351888859113954 34-35 7.21007391527442E-4 36-37 3.9054567041069783E-4 38-39 9.012592394093027E-4 40-41 9.012592394093027E-4 42-43 0.0012317209605260469 44-45 0.0010514691126441865 46-47 0.001442014783054884 48-49 2.1029382252883732E-4 50-51 9.913851633502329E-4 52-53 0.001081511087291163 54-55 0.0013218468844669773 56-57 0.0010214271379972097 58-59 6.308814675865118E-4 60-61 8.111333154683724E-4 62-63 9.913851633502329E-4 64-65 8.411752901153493E-4 66-67 3.304617211167443E-4 68-69 2.4033579717581404E-4 70-71 5.107135689986048E-4 72-73 2.4033579717581404E-4 74-75 3.304617211167443E-4 76-77 4.2058764505767464E-4 78-79 2.4033579717581404E-4 80-81 4.5062961970465134E-4 82-83 1.502098732348838E-4 84-85 3.9054567041069783E-4 86-87 3.004197464697676E-4 88-89 4.5062961970465134E-4 90-91 2.4033579717581404E-4 92-93 3.60503695763721E-4 94-95 3.004197464697676E-4 96-97 3.60503695763721E-4 98-99 4.5062961970465134E-4 100-101 4.806715943516281E-4 102-103 7.510493661744189E-4 104-105 4.5062961970465134E-4 106-107 4.806715943516281E-4 108-109 6.008394929395351E-4 110-111 7.810913408213957E-4 112-113 3.60503695763721E-4 114-115 4.5062961970465134E-4 116-117 6.909654168804655E-4 118-119 9.913851633502329E-4 120-121 5.407555436455815E-4 122-123 5.107135689986048E-4 124-125 3.9054567041069783E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1664338.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.77797269161896 #Duplication Level Percentage of deduplicated Percentage of total 1 55.08514991687923 18.606646896060834 2 14.47583564729505 9.779287623653929 3 9.639167541046799 9.767746139142561 4 6.498814990165913 8.780671810628329 5 4.221937370983399 7.13042426114014 6 2.764646768277848 5.6030497824517065 7 1.7263012713438468 4.08176700406717 8 1.1921571071034027 3.2214920166286567 9 0.8030780111060195 2.441371241554092 >10 3.281205153221866 18.953744684389527 >50 0.2034183502414029 4.748729585072805 >100 0.10594559879389426 6.352062116323832 >500 0.001981923765824484 0.40696697095605244 >1k 3.6034977560445164E-4 0.12603986793031025 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 6.008394929395351E-5 0.0 24-25 0.0 0.0 0.0 1.8025184788186054E-4 0.0 26-27 0.0 0.0 0.0 2.703777718227908E-4 0.0 28-29 0.0 0.0 0.0 4.5062961970465134E-4 0.0 30-31 0.0 0.0 0.0 0.001832560453465582 0.0 32-33 0.0 0.0 0.0 0.005798101106866514 0.0 34-35 3.0041974646976755E-5 0.0 0.0 0.011566160239086052 0.0 36-37 6.008394929395351E-5 0.0 0.0 0.022201019264115822 0.0 38-39 6.008394929395351E-5 0.0 0.0 0.042809813871941875 0.0 40-41 6.008394929395351E-5 0.0 0.0 0.06777469480357956 0.0 42-43 6.008394929395351E-5 0.0 0.0 0.09919860028431725 0.0 44-45 6.008394929395351E-5 0.0 0.0 0.13783257968032936 0.0 46-47 1.8025184788186054E-4 0.0 0.0 0.18433755643384936 0.0 48-49 1.8025184788186054E-4 0.0 0.0 0.23525870346047498 0.0 50-51 1.8025184788186054E-4 0.0 0.0 0.2845876258308108 0.0 52-53 1.8025184788186054E-4 0.0 0.0 0.33986485918124804 0.0 54-55 1.8025184788186054E-4 0.0 0.0 0.41163513661287554 0.0 56-57 1.8025184788186054E-4 0.0 0.0 0.5006194655172207 0.0 58-59 1.8025184788186054E-4 0.0 0.0 0.6013201645338868 0.0 60-61 1.8025184788186054E-4 0.0 0.0 0.7035830462321957 0.0 62-63 1.8025184788186054E-4 0.0 0.0 0.8063265995248561 0.0 64-65 1.8025184788186054E-4 0.0 0.0 0.9222886216621864 0.0 66-67 1.8025184788186054E-4 0.0 0.0 1.0720779072520124 0.0 68-69 1.8025184788186054E-4 0.0 0.0 1.255003490877454 0.0 70-71 2.703777718227908E-4 0.0 0.0 1.438289578198659 0.0 72-73 3.605036957637211E-4 0.0 0.0 1.6254510802493245 0.0 74-75 3.605036957637211E-4 0.0 0.0 1.8346333497162237 0.0 76-77 3.605036957637211E-4 0.0 0.0 2.0789947714947328 0.0 78-79 3.605036957637211E-4 0.0 0.0 2.3797449796856167 0.0 80-81 3.605036957637211E-4 0.0 0.0 2.6802848940539725 0.0 82-83 3.605036957637211E-4 0.0 0.0 2.9831079984954982 0.0 84-85 3.605036957637211E-4 0.0 0.0 3.315071818344591 0.0 86-87 3.605036957637211E-4 0.0 0.0 3.6792105930405965 0.0 88-89 3.605036957637211E-4 0.0 0.0 4.096974292481455 0.0 90-91 3.605036957637211E-4 0.0 0.0 4.547543828236812 0.0 92-93 3.605036957637211E-4 0.0 0.0 4.978075366902637 0.0 94-95 3.605036957637211E-4 0.0 0.0 5.436365690142267 0.0 96-97 3.605036957637211E-4 0.0 0.0 5.941761829628357 0.0 98-99 3.605036957637211E-4 0.0 0.0 6.4963066396369005 0.0 100-101 4.205876450576746E-4 0.0 0.0 7.098377853536961 0.0 102-103 4.806715943516281E-4 0.0 0.0 7.70276229948484 0.0 104-105 5.707975182925583E-4 0.0 0.0 8.32889713507713 0.0 106-107 7.210073915274422E-4 0.0 0.0 9.008236307769215 0.0 108-109 7.210073915274422E-4 0.0 0.0 9.730175000510714 0.0 110-111 7.210073915274422E-4 0.0 0.0 10.487292845563822 0.0 112-113 7.210073915274422E-4 0.0 0.0 11.226956303347036 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2320 0.0 61.031086 1 GTACATG 13930 0.0 57.015415 1 TACATGG 14150 0.0 55.71019 2 ACATGGG 14345 0.0 53.210983 3 CATGGGA 9230 0.0 49.438904 4 GTATCAA 3090 0.0 46.016613 2 ATGGGAG 3660 0.0 43.40156 5 CATGGGG 4930 0.0 42.720028 4 ATGGGAT 4180 0.0 39.283302 5 ATGGGGG 2915 0.0 38.166138 5 TCAACGC 3910 0.0 38.039818 5 ATCAACG 3950 0.0 37.805225 4 CAACGCA 3915 0.0 37.687305 6 AACGCAG 4190 0.0 35.63977 7 GAGTACA 8080 0.0 31.292461 1 AGTACAT 7750 0.0 30.860258 2 TACCTGG 1630 0.0 30.294634 2 TATCAAC 4915 0.0 29.656353 3 ACGCAGA 5075 0.0 28.955835 8 CGCAGAG 5225 0.0 27.669106 9 >>END_MODULE