FastQCFastQC Report
Thu 26 May 2016
SRR936229_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936229_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664338
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18200.1093527877149954No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31750.070.363761
GTACATG120000.049.1490861
GTATCAA45750.048.7993852
TACATGG122500.047.72542
ACATGGG123100.046.81573
CATGGGA81300.041.667354
CATGGGG42100.040.7272034
TCAACGC61150.038.5543065
CAACGCA61500.038.501886
ATCAACG62400.037.7819864
AACGCAG63450.037.412377
ATGGGAG32350.035.7027445
TATCAAC69800.033.861743
ATGGGAT35100.032.735915
ATGGGGG24900.031.5608545
ACGCAGA77950.030.3004028
CGCAGAG79700.029.485799
TACCTGG14600.029.359852
AGTACAT73450.028.6936382
GAGTACA78700.028.159811
Produced by FastQC (version 0.11.5)