FastQCFastQC Report
Thu 26 May 2016
SRR936228_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936228_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668476
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31400.057.7891241
GTACATG161400.056.5823251
TACATGG165300.054.2755742
ACATGGG166000.052.469743
CATGGGA116450.047.7181174
CATGGGG48000.046.6039284
GTATCAA42550.042.6457942
ATGGGAT47250.042.5589455
ATGGGAG41100.041.9790045
TCAACGC49700.037.947125
ATCAACG50000.037.838434
CAACGCA50300.037.257916
ATGGGGG30450.035.950635
AACGCAG52900.035.426717
GTCGGGA4550.033.9967882
AGTACAT89050.033.939522
GAGTACA96000.032.6599351
ATGGGGA21600.032.501565
TATCCCG1102.041637E-532.451485
ATGGGAC22550.031.9238155
Produced by FastQC (version 0.11.5)