##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936228_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1668476 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.78657948930641 31.0 30.0 31.0 22.0 34.0 2 28.901306341835305 31.0 30.0 33.0 22.0 34.0 3 29.17132940479815 31.0 30.0 34.0 23.0 34.0 4 32.53583569676759 35.0 33.0 37.0 23.0 37.0 5 32.94165993397568 35.0 33.0 37.0 26.0 37.0 6 32.88285896830401 36.0 33.0 37.0 25.0 37.0 7 32.721934867507834 35.0 33.0 37.0 25.0 37.0 8 32.723077826711325 36.0 33.0 37.0 25.0 37.0 9 33.98837322203016 38.0 34.0 39.0 22.0 39.0 10-11 33.81352623591829 38.0 33.5 39.0 20.5 39.0 12-13 33.81745886665436 38.0 34.0 39.0 21.5 39.0 14-15 34.85839682440742 39.0 34.0 41.0 22.0 41.0 16-17 34.89050576693941 39.0 34.0 41.0 23.0 41.0 18-19 34.910899827147645 39.0 34.0 41.0 23.0 41.0 20-21 35.008113991450884 39.0 34.0 41.0 24.0 41.0 22-23 34.927668423159815 39.0 34.0 41.0 24.0 41.0 24-25 34.902498747359864 39.0 34.0 41.0 24.5 41.0 26-27 34.98651883515256 39.0 34.0 41.0 25.0 41.0 28-29 34.88610294664113 39.0 34.0 41.0 25.0 41.0 30-31 34.81322925831717 39.0 34.0 40.5 25.0 41.0 32-33 34.63589467274327 38.0 33.0 40.0 24.5 41.0 34-35 34.60578605865472 38.0 33.0 40.0 24.0 41.0 36-37 34.50468271644303 38.0 33.0 40.0 24.0 41.0 38-39 34.25725092839214 38.0 33.0 40.0 23.0 41.0 40-41 34.34109750454906 38.0 33.0 40.0 23.5 41.0 42-43 34.25521613736128 38.0 33.0 40.0 23.0 41.0 44-45 34.1288328390699 38.0 33.0 40.0 22.5 41.0 46-47 34.09245802756527 38.0 33.0 40.0 23.0 41.0 48-49 34.00066347972641 38.0 33.0 40.0 22.5 41.0 50-51 33.868441320102896 38.0 33.0 40.0 22.0 41.0 52-53 33.69052926143379 38.0 32.0 40.0 20.5 41.0 54-55 33.51175024393518 38.0 32.0 40.0 20.0 41.0 56-57 33.310559456653856 38.0 32.0 40.0 18.5 41.0 58-59 33.07069715117269 37.0 31.5 40.0 17.5 41.0 60-61 32.82331421009352 37.0 31.0 40.0 15.0 41.0 62-63 32.569221553081974 36.5 31.0 40.0 13.0 41.0 64-65 32.27821407080474 36.0 31.0 39.5 11.0 41.0 66-67 31.9807773680892 36.0 31.0 39.0 8.0 41.0 68-69 31.693349799457707 35.0 30.5 39.0 7.5 40.5 70-71 31.35385165863938 35.0 30.0 38.5 7.0 40.0 72-73 31.024183446450536 35.0 30.0 38.0 2.0 40.0 74-75 30.674419949702603 35.0 30.0 37.0 2.0 39.5 76-77 30.344966604254424 34.5 30.0 37.0 2.0 39.0 78-79 30.0104463594322 34.0 29.0 36.0 2.0 39.0 80-81 29.683886672628198 34.0 29.0 36.0 2.0 37.5 82-83 29.374384168546626 34.0 29.0 35.0 2.0 37.0 84-85 29.39146412654422 34.0 29.0 35.0 2.0 37.0 86-87 29.265542027574867 34.0 29.0 35.0 2.0 36.0 88-89 29.08361702535727 34.0 29.0 35.0 2.0 36.0 90-91 28.900980895140236 34.0 29.0 35.0 2.0 36.0 92-93 28.771891834224768 34.0 29.0 35.0 2.0 35.5 94-95 28.64385642946018 34.0 29.0 35.0 2.0 35.0 96-97 28.53018652950357 34.0 29.0 35.0 2.0 35.0 98-99 28.40596898007523 34.0 29.0 35.0 2.0 35.0 100-101 28.293418065348256 34.0 29.0 35.0 2.0 35.0 102-103 27.339300655208703 32.5 26.0 34.5 2.0 35.0 104-105 27.989647738415176 34.0 27.5 35.0 2.0 35.0 106-107 28.11312958652087 34.0 29.0 35.0 2.0 35.0 108-109 28.0610695029476 34.0 28.5 35.0 2.0 35.0 110-111 27.94408520110568 34.0 28.0 35.0 2.0 35.0 112-113 27.8915872928349 34.0 28.0 35.0 2.0 35.0 114-115 27.771791742883927 34.0 27.5 35.0 2.0 35.0 116-117 27.638734389946276 34.0 27.0 35.0 2.0 35.0 118-119 27.422537093731044 34.0 27.0 35.0 2.0 35.0 120-121 27.205133606956288 34.0 27.0 35.0 2.0 35.0 122-123 26.88342535343631 34.0 26.5 35.0 2.0 35.0 124-125 24.87260739740937 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7980379592035902 1101 2 0.7297384511403564 1101 3 0.7145674133711459 1101 4 0.8621544868516153 1101 5 0.882770580367044 1101 6 0.7578508761295311 1101 7 0.7617952278447859 1101 8 0.7407673536107922 1101 9 0.920134168219235 1101 10-11 1.0214382775047142 1101 12-13 0.972342343447913 1101 14-15 1.034908539573756 1101 16-17 1.087709801814981 1101 18-19 0.972666192830971 1101 20-21 0.8886308536504828 1101 22-23 1.0049920467491944 1101 24-25 1.1643467499737241 1101 26-27 1.0093407906953331 1101 28-29 1.0599383764104786 1101 30-31 1.1126447083117625 1101 32-33 1.1359496542387575 1101 34-35 1.0639957495603483 1101 36-37 1.0218799141470285 1101 38-39 1.2972685078103012 1101 40-41 1.2704459562136137 1101 42-43 1.2733475534576613 1101 44-45 1.1603482603974413 1101 46-47 1.0101033414379117 1101 48-49 1.1338578968176733 1101 50-51 1.134691971955462 1101 52-53 1.0577298392974654 1101 54-55 1.1309695583600856 1101 56-57 1.097982102833349 1101 58-59 1.053121877916773 1101 60-61 1.096589084274818 1101 62-63 1.1091042098971045 1101 64-65 1.0137615290977067 1101 66-67 1.0777040430977003 1101 68-69 1.1576863932585226 1101 70-71 1.1412452151911943 1101 72-73 1.0741983556614016 1101 74-75 1.0304948058606413 1101 76-77 1.0240972114414966 1101 78-79 1.044948571704385 1101 80-81 1.03102517783282 1101 82-83 1.0452454058122527 1101 84-85 0.8723997149823006 1101 86-87 0.9963117735403806 1101 88-89 0.9758454889181927 1101 90-91 0.8705823260293073 1101 92-93 0.9284877300356662 1101 94-95 1.0104133961243527 1101 96-97 1.1343720624599456 1101 98-99 1.011807560311805 1101 100-101 1.1999039525379267 1101 102-103 1.2483399673804847 1101 104-105 1.268411853478181 1101 106-107 1.2456015174310906 1101 108-109 1.1606938138924825 1101 110-111 1.2507961568521502 1101 112-113 1.2373750280783398 1101 114-115 1.345794895229247 1101 116-117 1.2809896906892924 1101 118-119 1.2714704903835141 1101 120-121 1.3896613508287494 1101 122-123 1.369448941536941 1101 124-125 1.203667400268678 1102 1 0.5885085408500679 1102 2 0.5986517041299138 1102 3 0.6069298249445687 1102 4 0.746307359380836 1102 5 0.6637734074505701 1102 6 0.6019410989475844 1102 7 0.8208267854709703 1102 8 0.672847488536064 1102 9 0.7467889104972585 1102 10-11 0.8582638990722273 1102 12-13 0.8417475358074284 1102 14-15 0.9669982274277942 1102 16-17 0.9156017577530804 1102 18-19 0.8408698309362777 1102 20-21 0.7801868084358148 1102 22-23 1.0841358738945672 1102 24-25 1.1113088165332812 1102 26-27 0.8665079520724532 1102 28-29 0.9015498694442812 1102 30-31 0.9312584368767389 1102 32-33 1.0689502247892477 1102 34-35 0.9881813733213889 1102 36-37 0.9746529324758129 1102 38-39 0.9723723065798282 1102 40-41 0.9351582103314371 1102 42-43 1.1773907904508008 1102 44-45 1.169830234968856 1102 46-47 1.05188048663193 1102 48-49 1.0653908166477493 1102 50-51 0.9503369273673457 1102 52-53 1.16763103430322 1102 54-55 1.1101230259419665 1102 56-57 1.0956167685012659 1102 58-59 1.1171081969560035 1102 60-61 1.1305230561813318 1102 62-63 1.1338126049233992 1102 64-65 0.9832576420037 1102 66-67 0.9800686627832071 1102 68-69 1.083932386862294 1102 70-71 1.0388899588343499 1102 72-73 1.0204195962411973 1102 74-75 1.003486780233704 1102 76-77 1.0142285255627996 1102 78-79 1.076720154322782 1102 80-81 1.0113023906994876 1102 82-83 1.0072073936148378 1102 84-85 0.9621124358080912 1102 86-87 0.9222222478673565 1102 88-89 0.9405437709661513 1102 90-91 0.918432144071538 1102 92-93 0.9389487704210744 1102 94-95 0.8961677332998619 1102 96-97 1.080913143845649 1102 98-99 0.9064544344768386 1102 100-101 0.8976357226329128 1102 102-103 0.8948509752713498 1102 104-105 0.8804709165385951 1102 106-107 0.8538156360472549 1102 108-109 0.8997444802931227 1102 110-111 0.8732223353189461 1102 112-113 0.9380353177639762 1102 114-115 0.9894666414287876 1102 116-117 0.9544770026497353 1102 118-119 1.1295377418352714 1102 120-121 0.9799738643195255 1102 122-123 0.9149559584912765 1102 124-125 0.8126932990607223 1103 1 0.33557206600992373 1103 2 0.3654608506408934 1103 3 0.5184324660343655 1103 4 0.5423109735037173 1103 5 0.7041369048094808 1103 6 0.5912099340879777 1103 7 0.4098176111590419 1103 8 0.4881831849497402 1103 9 0.6977063416899156 1103 10-11 0.7618867097477846 1103 12-13 0.6734093373086765 1103 14-15 0.6955080967633549 1103 16-17 0.771759667121998 1103 18-19 0.8525757664380791 1103 20-21 0.5886765220860752 1103 22-23 0.8143659267163628 1103 24-25 0.8490207987406748 1103 26-27 0.7398607460674498 1103 28-29 0.8157370393080541 1103 30-31 0.8148888928122417 1103 32-33 0.9239526573719559 1103 34-35 0.8954183068771329 1103 36-37 0.7662639972520182 1103 38-39 0.8450822037163306 1103 40-41 0.8787900563142017 1103 42-43 0.90141080713137 1103 44-45 0.8957806797839822 1103 46-47 0.8274903698679594 1103 48-49 0.7289403023302583 1103 50-51 0.7430391792439153 1103 52-53 0.6873488547091142 1103 54-55 0.593746531641159 1103 56-57 0.6932901768971433 1103 58-59 0.7116539990132722 1103 60-61 0.7311607403625899 1103 62-63 0.6831870836556746 1103 64-65 0.7180922263795679 1103 66-67 0.7705729024273502 1103 68-69 0.6704037518887063 1103 70-71 0.6961668562488548 1103 72-73 0.780752860072166 1103 74-75 0.6720786623576984 1103 76-77 0.7144769965108111 1103 78-79 0.6735716972752392 1103 80-81 0.7282071029597041 1103 82-83 0.6264682359834808 1103 84-85 0.6025072092303887 1103 86-87 0.5571106965746146 1103 88-89 0.6082043214248714 1103 90-91 0.5580954051224118 1103 92-93 0.5922032185723118 1103 94-95 0.7165093374144043 1103 96-97 0.5699873723694147 1103 98-99 0.6225251183800928 1103 100-101 0.6422857782348075 1103 102-103 0.5841215479708204 1103 104-105 0.619413090572511 1103 106-107 0.5607398089691351 1103 108-109 0.4174154561063048 1103 110-111 0.42071503757081885 1103 112-113 0.4905478281893281 1103 114-115 0.48772213211546855 1103 116-117 0.5186780729861269 1103 118-119 0.525570894462458 1103 120-121 0.5373967166965059 1103 122-123 0.7423746407264709 1103 124-125 0.6639686674232443 1104 1 0.46698161174873576 1104 2 0.5068197965730121 1104 3 0.47595705526627086 1104 4 0.6137092906337429 1104 5 0.46922090617397316 1104 6 0.4221539900320366 1104 7 0.3035011633791811 1104 8 0.276815995228894 1104 9 0.6368870796511459 1104 10-11 0.7638790771364938 1104 12-13 0.6665250630463575 1104 14-15 0.8479478318250386 1104 16-17 0.8518992429932837 1104 18-19 0.667359509118171 1104 20-21 0.7222401129667375 1104 22-23 0.7352156054928685 1104 24-25 0.7137935260133972 1104 26-27 0.6533656354869706 1104 28-29 0.7969102867922757 1104 30-31 0.7280132176743734 1104 32-33 0.8223213110759815 1104 34-35 0.7552181107318106 1104 36-37 0.8839447920669627 1104 38-39 0.9266758133587203 1104 40-41 0.7869908494235318 1104 42-43 0.9792392794720399 1104 44-45 1.0323998025311525 1104 46-47 1.0083985369041812 1104 48-49 1.02074981395684 1104 50-51 0.9873751278481535 1104 52-53 1.0367867135981896 1104 54-55 0.9615150943792372 1104 56-57 0.8852435994908134 1104 58-59 0.8749169048066676 1104 60-61 0.9714156582089757 1104 62-63 0.9373942585945585 1104 64-65 1.0446898157820357 1104 66-67 1.0046250799246543 1104 68-69 0.9846193127506027 1104 70-71 1.1135903880016578 1104 72-73 1.1392002561719963 1104 74-75 1.0447711121842929 1104 76-77 0.9206019183369527 1104 78-79 0.8051709922891703 1104 80-81 0.8959536348830675 1104 82-83 0.8396039315886874 1104 84-85 0.8423964866196663 1104 86-87 0.8922217745210581 1104 88-89 0.8042409067907244 1104 90-91 0.8066644681731816 1104 92-93 0.7957967287206991 1104 94-95 0.8180781446234526 1104 96-97 0.8354103640119241 1104 98-99 0.8762778189315732 1104 100-101 0.9154973735500604 1104 102-103 0.9296155356904166 1104 104-105 0.9323558388811151 1104 106-107 0.9489877906622404 1104 108-109 0.9477341211810675 1104 110-111 1.0516409713930983 1104 112-113 1.0177651230895535 1104 114-115 0.9744858765011664 1104 116-117 0.9497639078829394 1104 118-119 0.9374387522144616 1104 120-121 0.9619816845741589 1104 122-123 1.175187051892543 1104 124-125 1.2033434186882417 1105 1 0.48608179290853926 1105 2 0.6145115427471417 1105 3 0.5630639662214207 1105 4 0.6334912952395939 1105 5 0.5244932459991531 1105 6 0.5312679213681903 1105 7 0.5144191824272824 1105 8 0.47846096272751737 1105 9 0.5991092597595014 1105 10-11 0.6811791714013609 1105 12-13 0.7259205606532362 1105 14-15 0.8047533268419116 1105 16-17 0.6857046727336638 1105 18-19 0.6646244944590336 1105 20-21 0.430969825490493 1105 22-23 0.3930509248458094 1105 24-25 0.6646726880036766 1105 26-27 0.5241433872945294 1105 28-29 0.5981280793379824 1105 30-31 0.6191046616113454 1105 32-33 0.6908367763394097 1105 34-35 0.7944619843930312 1105 36-37 0.7465249400129608 1105 38-39 0.7861409392195071 1105 40-41 0.672418470081503 1105 42-43 0.8357261831134082 1105 44-45 0.7865845518378478 1105 46-47 0.6546952894339881 1105 48-49 0.8248203995992469 1105 50-51 0.8243055466213889 1105 52-53 0.7588462359287149 1105 54-55 0.8642875903766623 1105 56-57 0.9059328069008856 1105 58-59 0.9356368834128261 1105 60-61 0.917232102128402 1105 62-63 0.8640138700380149 1105 64-65 0.8710383543256981 1105 66-67 0.8304564602275839 1105 68-69 0.8330627492699207 1105 70-71 0.7649706347748584 1105 72-73 0.7850948914975291 1105 74-75 0.8259273342619267 1105 76-77 0.8310524735029539 1105 78-79 0.7715889064035615 1105 80-81 0.7175519402212096 1105 82-83 0.6719859127511576 1105 84-85 0.7546069099412023 1105 86-87 0.8979987437126624 1105 88-89 0.83739904645293 1105 90-91 0.804468861912536 1105 92-93 0.7139320812733985 1105 94-95 0.7840434750874259 1105 96-97 0.7829783936937709 1105 98-99 0.7197309732248343 1105 100-101 0.7827435971648455 1105 102-103 0.8454828461376742 1105 104-105 0.8498096487468487 1105 106-107 0.9253558770574237 1105 108-109 0.9502905028702386 1105 110-111 0.9245800768525285 1105 112-113 0.8114700130004024 1105 114-115 0.8697039877084372 1105 116-117 0.9369323752682099 1105 118-119 0.9888962779267274 1105 120-121 0.9498265707930251 1105 122-123 1.0316703758555406 1105 124-125 1.1381222401879292 1106 1 -0.0672954974824087 1106 2 -0.11330269457734943 1106 3 0.06894951922501846 1106 4 0.2230930797499866 1106 5 -0.05302180632713771 1106 6 -0.07550089871086385 1106 7 0.004668459036253125 1106 8 -0.03176570048837846 1106 9 0.2394008583380156 1106 10-11 0.16270976109302637 1106 12-13 0.18432104442815245 1106 14-15 0.07367891560907225 1106 16-17 0.17901275875124867 1106 18-19 -0.026694493720128776 1106 20-21 0.06387428508554649 1106 22-23 -0.0044322481252550006 1106 24-25 -3.9302094446512825 1106 26-27 -0.9317397276635546 1106 28-29 -0.6426392645774399 1106 30-31 -0.41899532159322916 1106 32-33 -5.351872363163885 1106 34-35 -1.0467142843526602 1106 36-37 -0.273456405813171 1106 38-39 0.09339545130547577 1106 40-41 -0.5166229251529018 1106 42-43 -0.08133880184364273 1106 44-45 0.04085707031386221 1106 46-47 -0.05494001924409986 1106 48-49 0.0706875651553176 1106 50-51 0.11981201341555447 1106 52-53 0.16711640582367693 1106 54-55 0.09803127941597012 1106 56-57 0.15420307959662694 1106 58-59 0.25787856832315725 1106 60-61 0.19183368312103966 1106 62-63 0.2601717464739792 1106 64-65 0.2167669611014631 1106 66-67 0.27070955018979603 1106 68-69 0.26446059398212185 1106 70-71 0.2783649511976023 1106 72-73 0.22661847406579483 1106 74-75 0.17059994086876173 1106 76-77 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-1.498082164122355 2216 100-101 -1.5312383454909337 2216 102-103 -1.532486642226079 2216 104-105 -1.4532862530945714 2216 106-107 -1.543453913974588 2216 108-109 -1.4696010220392068 2216 110-111 -1.4945159008456024 2216 112-113 -1.6347244801474936 2216 114-115 -1.8066728546182276 2216 116-117 -1.5541617887650254 2216 118-119 -1.499038971478054 2216 120-121 -1.5519378734445368 2216 122-123 -1.69304813542211 2216 124-125 -1.761263492889558 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58640.0 3 9280.0 4 6065.0 5 5444.0 6 5810.0 7 6639.0 8 7130.0 9 7893.0 10 9143.0 11 10300.0 12 10746.0 13 10963.0 14 11054.0 15 10415.0 16 9829.0 17 8775.0 18 7980.0 19 7475.0 20 7145.0 21 7490.0 22 8582.0 23 11511.0 24 17405.0 25 25007.0 26 28693.0 27 27234.0 28 31067.0 29 37466.0 30 44314.0 31 53745.0 32 67785.0 33 87599.0 34 118887.0 35 167798.0 36 265441.0 37 347301.0 38 110182.0 39 243.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.22495900420986 17.792112985356592 9.115090286634892 21.867837723798655 2 19.105396497751535 20.911780905991172 37.69391257025145 22.288910026005844 3 17.159322135483002 29.7586119060819 29.76598390746041 23.316082050974693 4 10.793380989854759 16.15393052424771 36.925932607597275 36.12675587830026 5 10.76180612494426 43.932653902769985 34.0719203862733 11.233619586012457 6 30.132744847955966 37.618612088723516 19.178597800132337 13.07004526318818 7 28.92670015858917 34.91317153162613 20.96391766788815 15.196210641896549 8 28.139214157323174 34.082691070939454 19.679031628738272 18.099063142999107 9 28.920753613251165 13.750492974883155 18.114384557569277 39.2143688542964 10-11 24.183556930225002 27.721888960446023 28.648382337648638 19.446171771680337 12-13 24.835205143972985 26.582647961746257 28.0842021099975 20.497944784283266 14-15 22.616936982294625 27.835556994042264 25.186074249889117 24.36143177377399 16-17 22.46803577978792 30.10360650864167 25.77363242525299 21.65472528631742 18-19 21.863494981358215 28.91854285721992 27.403675132812022 21.81428702860984 20-21 23.343492645736685 27.94635043933782 27.068274175567304 21.641882739358195 22-23 23.125559413626537 28.250328149333743 26.75916838440145 21.864944052638272 24-25 22.584618372220145 28.258568524219147 27.118366226492896 22.03844687706781 26-27 22.460574781225734 28.203172597693598 26.973044037751354 22.363208583329314 28-29 22.28265104174783 28.33334132480164 27.132253606050185 22.25175402740035 30-31 22.58505721326464 27.81188294168751 27.365091142949698 22.237968702098144 32-33 22.2788419957691 28.231320344549506 27.261072397291002 22.228765262390397 34-35 22.580840435456366 28.34757753648357 27.05179907297553 22.019782955084537 36-37 22.741146414738118 28.01756107793852 26.851607393034005 22.389685114289353 38-39 22.41366703167545 28.067377384918846 27.203283520722486 22.315672062683216 40-41 22.3983369015111 27.995740962281207 27.42209545135251 22.18382668485518 42-43 22.373227765973706 27.96762854034745 27.138293440898558 22.520850252780285 44-45 22.219411890042622 28.012225744127843 27.404115145436187 22.36424722039334 46-47 22.535701731226418 28.040809342131563 26.935796070764244 22.487692855877775 48-49 22.320622390386884 28.105771123912586 27.042911456930163 22.53069502877036 50-51 22.358160183499763 27.905318052874257 27.19531732764981 22.541204435976166 52-53 22.455516280853907 27.888531104514826 27.322795592908978 22.33315702172229 54-55 22.183999637985945 28.01407419550018 27.346865506683326 22.455060659830554 56-57 22.358573034425074 28.35036361603992 26.961019797123015 22.330043552411986 58-59 22.531423504298733 27.822930954448015 27.018744570206053 22.626900971047192 60-61 22.49531902549423 28.04170442612811 27.40075255206011 22.062223996317552 62-63 22.101118433611568 27.947504554378185 27.480843741449775 22.47053327056048 64-65 21.995182366430242 28.160171032584657 27.335875007641803 22.508771593343297 66-67 22.194638742489005 28.35953647367454 27.082828254979635 22.36299652885682 68-69 22.211523794711262 28.073243172859918 27.1766430252396 22.538590007189224 70-71 22.038833322145397 27.896196741434036 27.44254595843842 22.622423977982145 72-73 22.223663994260615 28.10245784839597 26.953232344693934 22.720645812649483 74-75 22.00337195761139 28.359446126294372 27.154267959749877 22.48291395634436 76-77 21.675130022011217 28.320929235960545 27.362804489748825 22.641136252279413 78-79 22.12635592080047 28.154125682249507 27.15800318795159 22.561515208998426 80-81 22.020509869850432 28.226004141531284 26.928614025466523 22.82487196315176 82-83 22.258419679802838 28.151686093623386 27.083463192669697 22.506431033904075 84-85 21.754610669554747 27.960007599790703 27.47150527999655 22.813876450658 86-87 21.71540650327321 28.117029549571686 27.448601785770478 22.718962161384624 88-89 22.05113021515878 27.92913608342516 27.42672833211115 22.593005369304908 90-91 22.06750619879662 28.05685931233964 27.25795324935615 22.617681239507597 92-93 21.925526313502473 27.97562555121658 27.372917139280723 22.725930996000223 94-95 21.73395646004492 27.846751584912504 27.46208721476445 22.95720474027813 96-97 21.85794838845789 27.97180520526664 27.42372625360811 22.74652015266736 98-99 21.719878187581774 28.091655393843567 27.254839322563768 22.933627096010888 100-101 21.509043026297178 28.04479918104513 27.384313034472342 23.061844758185355 102-103 21.580853167319315 27.862658443882317 27.389315914408165 23.167172474390206 104-105 21.39667089709569 27.89907504940911 27.663799264894283 23.04045478860092 106-107 21.372142281763345 28.03329889047651 27.614260781173584 22.98029804658656 108-109 21.54722639938674 28.132868394444465 27.172073938708856 23.14783126745993 110-111 21.581950770141788 28.439506155080824 27.137975820802307 22.840567253975074 112-113 21.391405091327982 28.12604044017718 27.481405807555394 23.001148660939442 114-115 21.10280841550704 28.49096551176454 27.173858645733283 23.232367426995133 116-117 21.11316956164993 28.310191248102296 27.37363953967159 23.20299965057619 118-119 21.06540737486447 28.161270543755855 27.32984067257594 23.443481408803734 120-121 21.040982029568532 28.182794583997367 27.454549486563383 23.32167389987072 122-123 21.14033762830679 28.06737582276372 27.376679840475838 23.415606708453655 124-125 21.218902975335148 27.928143640796122 26.961298317168296 23.89165506670043 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 156.0 1 203.5 2 277.0 3 293.0 4 272.5 5 257.0 6 247.5 7 256.0 8 266.5 9 266.5 10 285.5 11 318.0 12 335.0 13 357.5 14 397.5 15 464.5 16 574.5 17 703.0 18 839.0 19 1082.5 20 1438.0 21 1903.5 22 2648.5 23 3908.5 24 5303.5 25 6855.0 26 9573.0 27 12554.0 28 14709.5 29 17284.5 30 21328.0 31 25864.0 32 30207.5 33 34480.0 34 39255.0 35 42791.0 36 47153.5 37 52730.5 38 53809.0 39 53614.0 40 53417.0 41 53949.0 42 55403.5 43 57009.0 44 60129.0 45 63098.0 46 64093.0 47 63397.0 48 62352.0 49 59773.0 50 57752.5 51 55680.0 52 53346.0 53 52049.0 54 49772.0 55 46401.5 56 41818.5 57 37988.0 58 35298.0 59 31672.5 60 27036.0 61 22396.0 62 18801.5 63 14690.5 64 10908.5 65 8501.5 66 6370.0 67 5028.5 68 3850.0 69 2602.5 70 1940.0 71 1532.0 72 1300.5 73 1092.5 74 816.5 75 649.0 76 466.0 77 295.0 78 205.5 79 133.0 80 84.0 81 54.0 82 33.0 83 25.5 84 20.0 85 12.5 86 9.0 87 5.0 88 3.0 89 5.0 90 4.5 91 2.5 92 3.0 93 3.5 94 2.0 95 1.5 96 1.5 97 1.0 98 0.5 99 1.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.192192156195234E-4 2 2.9967467317480143E-4 3 5.9934934634960286E-5 4 5.394144117146426E-4 5 0.0011986986926992057 6 7.192192156195234E-4 7 8.39089084889444E-4 8 0.0014983733658740072 9 0.00203778777758865 10-11 0.0020677552449061298 12-13 0.0022775275161284908 14-15 0.003356356339557776 16-17 0.0028768768624780937 18-19 0.0027869744605256534 20-21 0.003356356339557776 22-23 0.09301901855345837 24-25 0.002637137123938253 26-27 0.0019478853756362095 28-29 0.0017980480390488087 30-31 0.0015283408331914874 32-33 0.0021876251141760505 34-35 0.0011687312253817255 36-37 8.091216175719638E-4 38-39 0.0013785034966040865 40-41 0.0014384384312390469 42-43 0.0018579829736837688 44-45 0.0017680805717313285 46-47 0.0020677552449061298 48-49 3.296421404922816E-4 50-51 0.0016182432351439277 52-53 0.0017081456370963682 54-55 0.0019478853756362095 56-57 0.0015882757678264477 58-59 0.0011687312253817255 60-61 0.0011986986926992057 62-63 0.0015882757678264477 64-65 0.0013185685619691264 66-67 3.296421404922816E-4 68-69 3.895770751272419E-4 70-71 7.192192156195234E-4 72-73 2.697072058573213E-4 74-75 5.394144117146426E-4 76-77 5.094469443971625E-4 78-79 3.895770751272419E-4 80-81 6.592842809845632E-4 82-83 2.3973973853984114E-4 84-85 5.394144117146426E-4 86-87 5.094469443971625E-4 88-89 6.29316813667083E-4 90-91 4.19544542444722E-4 92-93 4.4951200976220217E-4 94-95 5.094469443971625E-4 96-97 7.192192156195234E-4 98-99 5.394144117146426E-4 100-101 4.794794770796823E-4 102-103 0.0013485360292866066 104-105 5.094469443971625E-4 106-107 7.192192156195234E-4 108-109 7.791541502544838E-4 110-111 0.001018893888794325 112-113 6.892517483020433E-4 114-115 5.094469443971625E-4 116-117 8.990240195244043E-4 118-119 0.0014983733658740072 120-121 7.791541502544838E-4 122-123 8.39089084889444E-4 124-125 5.094469443971625E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1668476.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.85036025465685 #Duplication Level Percentage of deduplicated Percentage of total 1 54.006054188308426 17.741183360184515 2 14.037481551748188 9.22272652086055 3 9.699398901383356 9.558862444921983 4 6.755649333177787 8.877020573960905 5 4.47702062107289 7.3535870134886 6 3.0398983900848124 5.991705435110249 7 1.94418320323676 4.470698303916621 8 1.2824881441555762 3.3704158046269574 9 0.9365967097340994 2.7690785395282296 >10 3.5084698469218134 19.325851690811007 >50 0.2058739855288269 4.6508489329934015 >100 0.10375237204991003 6.0141666340731295 >500 0.003132752597612834 0.6538547455238775 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.9934934634960286E-5 0.0 5 0.0 0.0 0.0 5.9934934634960286E-5 0.0 6 0.0 0.0 0.0 5.9934934634960286E-5 0.0 7 0.0 0.0 0.0 5.9934934634960286E-5 0.0 8 0.0 0.0 0.0 5.9934934634960286E-5 0.0 9 0.0 0.0 0.0 5.9934934634960286E-5 0.0 10-11 0.0 0.0 0.0 5.9934934634960286E-5 0.0 12-13 0.0 0.0 0.0 1.1986986926992057E-4 0.0 14-15 0.0 0.0 0.0 1.1986986926992057E-4 0.0 16-17 0.0 0.0 0.0 1.1986986926992057E-4 0.0 18-19 0.0 0.0 0.0 1.1986986926992057E-4 0.0 20-21 0.0 0.0 0.0 1.1986986926992057E-4 0.0 22-23 0.0 0.0 0.0 2.697072058573213E-4 0.0 24-25 0.0 0.0 0.0 4.794794770796823E-4 0.0 26-27 0.0 0.0 0.0 7.192192156195234E-4 0.0 28-29 0.0 0.0 0.0 9.289914868418844E-4 0.0 30-31 0.0 0.0 0.0 0.002697072058573213 0.0 32-33 0.0 0.0 0.0 0.009110110064513965 0.0 34-35 0.0 0.0 0.0 0.017920545455853126 0.0 36-37 5.9934934634960286E-5 0.0 0.0 0.032274962300926115 0.0 38-39 5.9934934634960286E-5 0.0 0.0 0.05618900122027527 0.0 40-41 5.9934934634960286E-5 0.0 0.0 0.08306981940405495 0.0 42-43 5.9934934634960286E-5 0.0 0.0 0.11480536729326643 0.0 44-45 5.9934934634960286E-5 0.0 0.0 0.15622040712602397 0.0 46-47 5.9934934634960286E-5 0.0 0.0 0.2026999489354357 0.0 48-49 1.1986986926992057E-4 0.0 0.0 0.2521462700092779 0.0 50-51 1.1986986926992057E-4 0.0 0.0 0.3040199559358361 0.0 52-53 1.1986986926992057E-4 0.0 0.0 0.3632656388224943 0.0 54-55 1.1986986926992057E-4 0.0 0.0 0.43437843876687465 0.0 56-57 1.1986986926992057E-4 0.0 0.0 0.529645017369144 0.0 58-59 1.1986986926992057E-4 0.0 0.0 0.6380073791891523 0.0 60-61 1.1986986926992057E-4 0.0 0.0 0.7378889477583136 0.0 62-63 1.1986986926992057E-4 0.0 0.0 0.8424454412290018 0.0 64-65 1.1986986926992057E-4 0.0 0.0 0.9627947899760021 0.0 66-67 1.1986986926992057E-4 0.0 0.0 1.1168575394551674 0.0 68-69 1.1986986926992057E-4 0.0 0.0 1.306551607574817 0.0 70-71 1.1986986926992057E-4 0.0 0.0 1.4937883433744328 0.0 72-73 1.1986986926992057E-4 0.0 0.0 1.676559926543744 0.0 74-75 1.1986986926992057E-4 0.0 0.0 1.8733263169503187 0.0 76-77 1.1986986926992057E-4 0.0 0.0 2.1128562832189375 0.0 78-79 1.1986986926992057E-4 0.0 0.0 2.4119316070473893 0.0 80-81 1.1986986926992057E-4 0.0 0.0 2.721525511904277 0.0 82-83 1.1986986926992057E-4 0.0 0.0 3.021589762154205 0.0 84-85 1.1986986926992057E-4 0.0 0.0 3.3250403362110093 0.0 86-87 1.1986986926992057E-4 0.0 0.0 3.6768883699855435 0.0 88-89 1.1986986926992057E-4 0.0 0.0 4.0852250796535285 0.0 90-91 1.1986986926992057E-4 0.0 0.0 4.526466068436106 0.0 92-93 1.1986986926992057E-4 0.0 0.0 4.961833433624458 0.0 94-95 1.1986986926992057E-4 0.0 0.0 5.412873784219851 0.0 96-97 1.1986986926992057E-4 0.0 0.0 5.9041604434226205 0.0 98-99 1.1986986926992057E-4 0.0 0.0 6.450347502751013 0.0 100-101 1.1986986926992057E-4 0.0 0.0 7.05095548272795 0.0 102-103 1.1986986926992057E-4 0.0 0.0 7.651983007247333 0.0 104-105 1.1986986926992057E-4 0.0 0.0 8.258704350557036 0.0 106-107 1.1986986926992057E-4 0.0 0.0 8.884964482557734 0.0 108-109 1.1986986926992057E-4 0.0 0.0 9.57370678391538 0.0 110-111 1.1986986926992057E-4 0.0 0.0 10.293914925956383 0.0 112-113 1.1986986926992057E-4 0.0 0.0 11.008099607066569 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3140 0.0 57.789124 1 GTACATG 16140 0.0 56.582325 1 TACATGG 16530 0.0 54.275574 2 ACATGGG 16600 0.0 52.46974 3 CATGGGA 11645 0.0 47.718117 4 CATGGGG 4800 0.0 46.603928 4 GTATCAA 4255 0.0 42.645794 2 ATGGGAT 4725 0.0 42.558945 5 ATGGGAG 4110 0.0 41.979004 5 TCAACGC 4970 0.0 37.94712 5 ATCAACG 5000 0.0 37.83843 4 CAACGCA 5030 0.0 37.25791 6 ATGGGGG 3045 0.0 35.95063 5 AACGCAG 5290 0.0 35.42671 7 GTCGGGA 455 0.0 33.996788 2 AGTACAT 8905 0.0 33.93952 2 GAGTACA 9600 0.0 32.659935 1 ATGGGGA 2160 0.0 32.50156 5 TATCCCG 110 2.041637E-5 32.45148 5 ATGGGAC 2255 0.0 31.923815 5 >>END_MODULE