FastQCFastQC Report
Thu 26 May 2016
SRR936228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936228_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668476
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60180.360688436633191No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43490.2606570307274423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39800.23854103984714195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48450.078.933871
GTATCAA71800.053.321242
GTACATG142450.048.1739461
TACATGG147700.046.2780572
ACATGGG147500.045.7339323
CATGGGG43950.041.0472874
CATGGGA103600.040.3439144
ATGGGAT38500.038.8162575
ATCAACG102400.038.665394
TCAACGC102750.038.533685
CAACGCA104150.038.101476
AACGCAG106750.037.2292067
TATCAAC110900.035.862923
TAACGCA1606.9394446E-933.4906234
ATGGGAG40450.032.676475
ACGCAGA124900.031.9620976
AGTACAT89250.031.8217352
GAGTACA94350.031.5682321
CGCAGAG126450.031.4762127
GTCGGGA4400.031.123532
Produced by FastQC (version 0.11.5)